Use Instructions

PN 10232 Eagle V1.2 Instructions for Use Rev 1.0 Page 12 of 101
1.4.2.4. Optical Filters – Dual Bandpass Filter
In order to effectively visualize and image PpIX fluorescence, the 405 nm excitation light must be blocked
from the imaging sensor. To accomplish this, a custom dual bandpass emission filter is placed in front of
the camera image sensor thus preventing reflected or backscattered excitation light as well ambient room
lighting from entering the image sensor. The two wavelength bands of fluorescence emission transmitted
to the device’s imaging sensor are green (500 – 545 nm) and red (600 – 660 nm) (Figure 5).
Figure 5 – Dual Bandpass Filter
First, the red bandpass region is for visualizing the red fluorescence emitted from fluorophores such as
PpIX. Second, the green bandpass region is for visualizing green autofluorescence emitted from
components of healthy tissue such as collagen and elastin in connective tissue and redox cofactors NADH
and FAD. The addition of the green fluorescence channel is to (visually) contrast and accentuate the red
fluorescence produced by the accumulation of PpIX in cancerous cells and differentiate the PpIX
fluorescent cells from healthy tissue, seen as green, in real-time.
1.4.2.5. Detector – C-MOS Camera Sensor
Once fluorescence is induced, an image sensor is required to detect this light information from the
fluorescence source and generate an image for real-time visualization and documentation. The Eagle V1.2
Imaging System utilises a C-MOS (Complementary Metal Oxide Semiconductor) sensor as a detector.
1.4.2.6. Display
Finally, colour display screens present all the captured fluorescence information in a real-time visual form
to the user e.g. images or video. The Eagle V1.2 Imaging System uses two forms of display, a colour active
matrix organic light emitting diode (AMOLED) display on the Handheld Fluorescence Camera (HFC) and an
external colour liquid crystal display (LCD) monitor, which is larger for viewing by multiple members of
the surgical team.
1.1. Regulatory Information