Instructions for Use Leica ASP300 S Advanced Smart Processor Vacuum Tissue Processor Leica ASP300 S, V 1.5 RevD, English – 03/2013 Order No.: 14 0476 80101 RevD Always keep this manual with the instrument. Read carefully before working with the instrument.
WARNING The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Contents 1. Important Information................................................................................................................ 7 2. Safety.......................................................................................................................................... 10 3. Instrument Components and Specifications........................................................................ 15 4. Initial Operation...................................................................
Contents 5.1.4 5.1.5 5.1.6 5.1.7 5.1.8 5.1.9 5.2 5.2.1 5.2.2 5.3 5.3.1 5.3.2 5.3.3 5.3.4 5.4 5.5 5.6 Editing the reagent list................................................................................................................ 45 Viewing the program list............................................................................................................ 48 Adding and/or modifying programs..........................................................................................
1. Important Information 1.1 Symbols and their meanings Warnings appear in a gray box and are marked by a warning triangle . Notes, i.e. important user information appear in a gray box and are marked by an – information symbol . Inflammable solvents and reagents are marked with this symbol. Instrument surfaces which become hot during operation are marked with this symbol. Avoid direct contact with these surfaces – they may cause burns. (5) Numbers in parentheses refer to item numbers in illustrations.
1. Important Information 1.1 Symbols and their meanings (continued) SN Serial number REF Order No. Observe the Instructions for Use The package contents are fragile and must be handled with care. Indicates the correct upright position of the package. The package must be kept in a dry environment. Indicates the temperature range to which the medical device can be safely exposed. Minimum –29 °C Maximum +50 °C Indicates the air humidity range to which the medical can be safely exposed.
1. Important Information 1.2 Qualification of personnel 1.4 Instrument type • The Leica ASP300 S may be operated by trained laboratory personnel only. • All laboratory personnel designated to operate this instrument must read these Instructions for Use carefully and must be familiar with all technical features of the instrument before attempting to operate it. All information provided in these Instructions for Use applies only to the instrument type indicated on the cover page.
2. Safety 2.1 Safety notes • The safety and caution notes in this chapter must be observed at all times. • Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. • The protective devices located on the instrument and the accessories must not be removed or modified. Only qualified service personnel authorized by Leica may repair the instrument and access its internal components.
2. Safety 2.2 Warnings (continued) Warnings – Markings on the instrument itself Markings on the instrument showing the warning triangle indicate that the correct operating instructions (as defined in these Instructions for Use) must be followed when operating or replacing the item marked. Failure to adhere to these instructions may result in an accident, personal injury, damage to the instrument or accessory equipment. Some instrument surfaces become hot during operation.
2. Safety 2.2 Warnings (continued) Warnings – Operating the instrument The instrument may only be operated by trained laboratory personnel. It must only be operated for the purpose of its designated use and according to the instructions contained in these Instructions for Use. In an emergency, the instrument can be switched off while working with specimens via the ON/STOP switch located on the side of the instrument.
2. Safety 2.2 Warnings (continued) Warnings – Handling reagents Take care when handling solvents! Always wear rubber gloves and safety goggles when handling the chemicals used in this instrument. Reagents used for tissue infiltration can be both toxic and/or flammable. To prevent damage to the instrument, use only the reagents listed in Chap. 3.5! Do not use acetone, benzene or trichlorethane! Use caution when handling paraffin wax or removing baskets – molten paraffin is hot and may cause burns.
2. Safety 2.3 Safety features on the instrument Overpressure protection Overcurrent protection Overheating protection Over vacuum protection Overcurrent protection Leica ASP300 S In the case of power failures or other processing problems the Leica ASP300 S incorporates specimen protection features such as a fluid level sensor and sophisticated software controls which ensure that processing can be completed successfully without damage to the tissue specimens.
3. Instrument Components and Specifications 3.1 Overview – instrument components 4 3 1 23 6 22 7 5 10 9 21 20 12 11 13 8 19 17 18 2 15 14 16 15 Fig. 2 Instructions for Use, V 1.
3. Instrument Components and Specifications Overview – instrument components Instrument Components and Accessories 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Three wax baths and the retort comprise the infiltration module. The touchscreen and the electronic components are there. The cassettes to be processed are stored in three baskets (21), each of which can hold up to 100 cassettes.
3. Instrument Components and Specifications 3.2 Specific instrument options • Optionally usable Reagent Management System (RMS), displays service life and usage frequency of the individual reagents and enables automatic definition of the reagent sequence – there is no more need to rearrange reagent containers. If one or more reagents within a sequence are replaced, the RMS automatically uses the reagents in the correct sequence, organized in ascending order of cleanliness.
3. Instrument Components and Specifications 3.3 Standard delivery – packing list (continued) 1 1 1 1 1 1 1 Accessory kit, consisting of: 1 Basket handle 3 Specimen basket assemblies (lid, handle, separating walls and spiral inserts) 1 Stirrer 1 Reagent bottle, plastic 2 Set of adhesive bottle labels, 24 pcs.
3. Instrument Components and Specifications 3.4 Technical Data Nominal supply voltages: Two factory-preset voltages (not user-adjustable): 100 to 120 V or 230 to 240 V Nominal frequency: 50 to 60 Hz Main fuses: 2 melting fuses, 20 x 5 mm, UL-approved • for 100 to 120 V F 10 A 250 VAC • for 230 to 240 V F 5 A 250 VAC Nominal power: 1000 VA Dimensions, (L x W x H), in mm: 595 x 680 x 1325 mm Empty weight, unpacked: approx.
3. Instrument Components and Specifications Retort Capacity: max. 300 cassettes Reagent volume: 4.3 l Temperature (paraffin): 40 to 65 °C Temperature (processing reagents): Ambient temperature or 35 to 55 °C Temperature (cleaning reagents): 50 to 65 °C Temperature accuracy: +1K Filling time: approx. 90 sec Drain time: approx.
3. Instrument Components and Specifications 3.4 Technical data (continued) Hardware and Software: • Large color LCD touchscreen. • User-friendly, intelligent software. • 3.5" disk drive and printer port. • Alarm system with two remote alarm sockets. • Password-protected instrument supervisor mode. • Built-in multiple specimen protection system. Capacities: • 15 programs that consist of up to 10 reagent and 3 paraffin processing steps each. • Time per program step: 0 to 99 hours, 59 minutes.
3. Instrument Components and Specifications 3.5 Compatible reagents (continued) The following reagents may be used in the Leica ASP300 S: Fixatives Dehydration Formalin 1st (buffered or 2nd Isopropanol unbuffered) 3. Methanol 2nd Formalin replacement 4. Butyl alcohol 5. Industrial methylated spirits 1st * Ethanol Clearing 1st Xylene Paraffinizing 1st Wax 2nd Toluene* 3.
3. Instrument Components and Specifications 3.6.
4. Initial Operation 4.1 Installation site requirements • The instrument requires an installation area of approx. 650 x 700 mm with vibration-free floor. • Room temperature consistently between +15 °C and +35 °C. • Relative air humidity maximum 80 %, noncondensing. • Avoid vibrations, direct sunlight and heavy variations in temperature. The chemicals to be used in the Leica ASP300 S are both flammable and noxious.
4. Initial Operation 4.2 Electrical connection Notice! Observe the following instructions carefully to prevent damage to the instrument (refer also to Chap. 2.2 "Warnings – Transport and installation"). Severe damage may occur if the instrument is connected to a power supply voltage other than that to which it was originally set. The power supply voltage for the instrument is factory preset and CANNOT be altered by the user. • Check the voltage label (Fig.
4. Initial Operation 4.2.2 Connecting an uninterruptible power supply (UPS) An uninterruptible power supply (UPS) protects machines and instruments against malfunctions in the power supply. Leica recommends using an active tracking UPS (with an output power of 1000-1500 VA) to protect the instrument and the specimens from temporary power failure, voltage spikes, undervoltages and excess voltages. • Connect the ASP to a grounded socket using the power cable (37).
4. Initial Operation 4.3 Installing accessories 13 • Move the instrument to its final setup location. Active carbon filter • Unpack the active carbon filter (13) and insert it (see Fig. 5). When doing so, make sure that the filter is inserted with the correct side up. Note the direction of the label (29) on the front of the filter – the arrow must be pointing upwards. 18 29 Fig.
4. Initial Operation 4.3 Installing accessories (continued) Magnetic stirrer • Unpack the magnetic stirrer (30, Fig. 7) and insert it into the retort. • Important! The magnetic stirrer must be inserted such that the outer curvature (32) of the two stirrer blades (31) faces downwards! (Fig. 7) Do not insert them the other way around, as then there will be no effective stirring. 31 30 32 Fig.
4. Initial Operation 4.4 Making the data connections Printer (optional) • Swing the door of the instrument console (6) upwards. • Connect the printer to the printer port (8) using a Centronics standard cable (Fig. 8). • Your Leica dealer can recommend a suitable printer. • If compliance with the electromagnetic interference standards is of fundamental importance, a specially shielded printer cable must be used.
4. Initial Operation 4.5 Anti-reflection clip – Function The anti-reflection clip (Order no. 14 0476 44135) prevents any reflections that might otherwise be emitted from the specimen basket (3, order no. 14 0476 34193) and that could affect the level sensor in the retort, thus causing malfunctions. Installing the clip The anti-reflection clips are intended for installation on the specimen basket to prevent reflections of the level sensor. 1 4 3 2a Fig.
4. Initial Operation 4.6 Alarm functions The Leica ASP300 S is equipped with 3 different alarm functions: Instrument alarm This instrument alarm is generated from within the instrument itself for all alarm messages. Local alarm This alarm is external to the Leica ASP300 S, e.g. in the office of the instrument operator. The local alarm is used when the instrument cannot continue with the current program or operation because of a problem.
4. Initial Operation 4.7 Switching the instrument on The instrument MUST be connected to a grounded power socket. For additional electrical fuse protection, we recommend connecting the Leica ASP300 S to a socket with a residual current circuit breaker. • C onnect the power cable to the power socket. If applicable, switch on the switch for the power socket. • T o switch on the instrument, press in the ON/OFF switch on the rear panel of the instrument (Item 42 in Fig. 4) switching on (ON).
4. Initial Operation 1 2 6 3 7 4 8 SYSTEM DIAGNOSTICS menu If TOUCH HERE ... is touched during initialization (Fig. 13) and the password for supervisor mode is entered, the SYSTEM DIAGNOSTICS menu (Fig. 15) opens. This menu provides access to basic instrument settings. Notice! Only experienced operators may adjust the settings, since incorrect use of the functions can result in serious malfunctions. 5 Fig. 15 The following functions can be selected: 1 - Access for service technicians only.
4. Initial Operation 4.8 Touchscreen functions The Leica ASP300 S is programmed and operated via a color LCD touchscreen. The instrument's control software contains an online help feature including detailed information about all screen functions and error messages. The software functions are also explained here in detail. Help can be accessed from any screen by pressing the HELP button. Fig. 17 Button symbols Pressing a button on the Leica ASP300 S activates the corresponding instrument function.
4. Initial Operation 4.9 Checklist for initial operation When the instrument is prepared to the point where it can be switched on, the menus listed below have to be opened and the corresponding parameters configured. For detailed instructions about the individual parameters, refer to the online help. Screen display → → → → → → → → → → → → or → → → Parameter selection Press button Set warning values for the age of the carbon filter and the operating hours of the pressure air pump.
4. Initial Operation 4.10 Switching off the instrument If the instrument must be completely switched off or disconnected from the power supply, please proceed as follows: • Press MENU to switch to the MENU FUNCTIONS window. • Press the EXIT APPLICATION button there. A message appears stating that all data has been stored and the system is being shut down. Fig. 19 Once all data has been stored, the following message appears: Fig.
5. Operation 5.1 Setting up the instrument parameters → From the start screen, press MENU to switch to the MENU FUNCTIONS window, and press the MORE button there. Fig. 22 Fig. 21 In the MORE MENU FUNCTIONS press SYSTEM SETUP 5.1.1 System setup The SYSTEM SETUP is divided into five areas: • Program options • Display/Buzzer • Date/Time • Security • Instrument Fig. 23 37 Instructions for Use, V 1.
5. Operation Fig. 24 PROGRAM OPTIONS Select the options with which programs are executed here. Press a button to change the associated value. • STATION SEQUENCE: By Age or Sequential By Age - RMS is activated, reagents will automatically be used in order of increasing cleanliness. Sequential - reagents will be used in sequential order of stations. • WAX BATH ORDER: Auto or 1. ; 2. ; 3.
5. Operation DISPLAY/BUZZER: • The number next to DISPLAY OFF indicates the remaining time (in min) until the screen saver (after the last user action) is activated. • The BUZZER VOLUME can be set between 1 and 10. INSTRUMENT Press PARAFFIN BATH SET TEMP. to open the input screen (Fig. 28) for the wax bath temperature. Set the temperature according to the value required by the paraffin used.
5. Operation SECURITY Access rights to the instrument are managed here as user profiles. Supervisor mode is disabled in factory condition. Fig. 29 SUPERVISOR MODE: Enabled or Disabled Enabled - There are two different user profiles. A password is required for supervisor level access to the in- strument. Enabling SUPERVISOR MODE already requires entering the password. 5.1.2 Access levels The Leica ASP300 S may be configured to allow two levels of user access.
5. Operation LOCK MODE: Enabled or Disabled In the Leica ASP300 S, there is a lock mode to protect against operation by unauthorized persons. Enabled: The LOCK key is active in the menu functions. A password is required for activation. If LOCK is pressed, no keys can be enabled until the agreed password is entered. Disabled: All functions are accessible as long as the screen saver is active. Fig. 30 Entry keyboard The keyboard is displayed any time text needs to be entered.
5. Operation Use this menu to set the name of the instrument, the language of the user interface and configure a connected printer. The serial number of the instrument and the current software version are entered at the factory and cannot be edited. 5.1.3 INSTALLATION menu → → Select a language Press the LANGUAGE key, the SELECT THE LANGUAGE selection menu appears. Select the desired language there and press OK. ↓ Fig. 32 Assigning an instrument name ↓ ↓ Fig.
5. Operation Setting the altitude of the installation location (in meters above sea level) This is important as it affects the actual pressure in the retort. Press SITE ELEVATION (meters), enter the value in the number field and press OK. → This parameter (in meters) must be entered to ensure that the ASP300 S makes the appropriate corrections when calculating the proper pressure or vacuum. Fig. 35 Upgrading software New versions of the ASP300 S operating software are distributed on diskettes.
5. Operation Configuring a printer On the INSTALLATION screen press PRINTER TYPE to display the list of the printers that can be connected. → → • The Leica ASP300 S works with any printers that are compatible with commercial PCs. • Connect the printer provided to the printer port using a Centronics standard cable. (For additional information, refer to Chap. 4.4, Fig. 8) • In the SELECT THE PRINTER TYPE window, highlight the printer that is connected to the instrument and press OK.
5. Operation 5.1.4 Editing the reagent list Adding new reagents 1. Enter the reagent name and allocate the new reagent to the reagent group it belongs to. 2. Determine how many stations (bottles) work with the new reagent. 3. Fill the stations (bottles) according to the reagent list. → Entering reagent names Takes place via the REAGENTS menu option. You must be logged on at supervisor access level to proceed. • From the start screen, press MENU in the MENU FUNCTIONS window; there, press the MORE button.
5. Operation Allocation of reagents to the correct reagent group is the basis for compatibility monitoring. Allocation to the wrong reagent group can lead to reagent cross contamination. Changeable parameters Entering / modifying reagent thresholds If warning thresholds are required for a certain reagent, enter them as follows: • Highlight the reagent to be modified, either by pressing the reagent name or using the UP/DOWN buttons.
5. Operation Deleting reagents • Highlight the reagent to be deleted in the SET UP REAGENTS AND WARNING THRESHOLDS screen. • Press CLEAR. • Press YES in the screen to confirm the reagent is deleted. ↓ Please remember that a reagent which is already used in a program cannot be deleted. Fig. 42 ↓ ↓ Adding new reagents to stations Go to SMART SCREEN to remote-fill the reagent container from an external bulk container or fill the reagent container manually.
5. Operation 5.1.5 Viewing the program list → This list (Fig. 43) shows all programs defined in the ASP300 S. You can create: • Up to 15 infiltration programs • 3 retort cleaning programs • 1 paraffin cleaning program At supervisor access level: • The program names for the infiltration programs can be edited. • New infiltration programs can be added and existing ones can be deleted. Fig. 43 Important! New infiltration programs are created by copying an existing program.
5. Operation 5.1.6 Adding and/or modifying programs Creating a new program • Make sure you are logged on at supervisor level. • In the VIEW/EDIT PROGRAMS screen (Fig. 43) highlight a program as similar as possible to the program you wish to create. (This minimizes the number of operating steps). • Press COPY to copy the selected program. The new program will have the same name as the program copied, however, the figure "(2)" will be added to indicate the change.
5. Operation Editing program steps • To edit a step, highlight the corresponding line and press the respective headline. • In the entry windows that pop up, enter / select the program step settings. ↓ ↓ ↓ ↓ ↓ Fig.
5. Operation Editing program steps (continued) Setting a delay step A delay step is a step that is extended to ensure that a program will finish at a specified time. • Highlight the program step you wish to define as delay step. • Touch the DELAY button. The delay symbol is moved to the selected step, thus defining the step as delay step. Copying program steps • Highlight the step you wish to copy. • Touch the COPY button. • If required, modify properties of the step.
5. Operation 5.1.7 Favorites Up to five favorites can be configured in the ASP300 S. Favorites can be programmed so that they: • end "as soon as possible" (NOW) or • end at a predefined time. ↓ Fig. 46 Adding/modifying a Favorite • Select the program line to be modified. • Touch the PROGRAMS button. • Select the program you want and press OK to confirm. ↓ Fig. 47 Setting the end time • Press the END TIME button. • In the input window, specify the end time using 24-hour notation.
5. Operation Assigning a day for the end time If an end time has been assigned to a Favorite program, a specific weekday can also be set on which the program is to end. • Press the DAY button. • Select the desired day from the list and confirm with OK. ↓ Fig. 48 Assigning a symbol to a "Favorite" A corresponding symbol is displayed, which helps the operator recognize a certain program. • Press the ICON key in the table header. • Select the symbol you want and press OK to confirm.
5. Operation 5.1.8 Configuring the stations → → This window (Fig. 50) shows a list of all stations with the allocated reagents defined in the instrument. ↓ Fig. 50 Adding/changing a reagent Select the desired station. either by pressing the reagent name or using the UP/DOWN buttons. • Touch the REAGENT NAME button. • The SELECT REAGENT field appears. Only those reagents that are compatible with the selected station will be offered for selection.
5. Operation 5.1.9 Reagent groups → → In this window (Fig. 51), the colors used in the graphical program display for each reagent group are displayed. ↓ Fig. 51 Changing the color of a reagent group • Highlight the line containing the reagent to be modified. • Press the COLOR button in the table header; the SELECT COLOR window appears. • Select the color you want and confirm with OK. • To exit the display without changing any colors, select CANCEL.
5. Operation 5.2 Reagent handling 5.2.1 Draining/filling reagents (other than paraffin) ↓ ↓ 1. Manually changing the reagents • Remove the appropriate reagent container from the reagent cabinet, remove the bottle screw cap. • Drain/refill the reagent by pouring to/from a bulk container. Use the included funnel for clean filling. • On the REAGENT STATUS screen, verify correct allocation and set the reagent container as full. 2.
5. Operation Filling/draining reagents From the FAVORITES screen, press MENU. In the MENU FUNCTIONS window, tap the SMART SCREEN button. → The SMART SCREEN (Fig. 53) pops up. The smart screen is the initial screen for manual operation of the instrument. The following steps should only be carried out by trained laboratory personnel, experienced in handling reagents. Status bar The following values are displayed.
5. Operation 5.2.2 Replacing the paraffin Draining the paraffin • Connect the paraffin drain hose (36) to the spout (17) of the paraffin drain at the front of the instrument (Fig. 55) and into a catch tank. 17 When connecting the hose, be sure to press it onto the O-rings of the drain opening as far as it will go. The paraffin drain hose must be securely hooked into the external receptacle and remain there through the entire drainage process.
5. Operation • Fill the paraffin reservoir to the brim (1 in Fig. 58) with paraffin pellets. This corresponds to approx. 3.5 kg/bath (Leica Histowax pellets). • Press BACK and REAGENT STATUS to switch to the status table (Fig. 57). Select the filled wax bath and press the SET AS FULL button. • After approx. 90 min, add another 0.5 kg of paraffin pellets. • Do not insert the baffle plate during the heating phase. • The pellets need a total melting time of approx. 10 hours.
5. Operation Filling with liquid paraffin The temperature of filled liquid paraffin must not be greater than 70 °C to prevent damage to the thermal fuse. → • If filling with already melted paraffin, the liquid level must not exceed the MAX level mark (see Fig. 58) in the paraffin reservoir. • After a paraffin reservoir is filled with paraffin, it must be marked as full in the REAGENT STATUS table. → Fig.
5. Operation 5.3 Running programs There are two different windows from which a program can be started: ALL PROGRAMS FAVORITES Fig. 61 Fig. 62 Up to five favorites can be configured in the ASP300 S. A "Favorite" is an infiltration program that is used frequently and thus has been assigned to the FAVORITES list. All settings have already been programmed and only the number of blocks needs to be entered (if this function has been activated). In the Start screen, press the ALL PROGRAMS button.
5. Operation 5.3.1 Editing a program that has been called up. Resetting the end time: • Touch the FINISH TIME button and reset the end time in the input window. ↓ Fig. 63 To modify a program before the start, press the EDIT button. A message appears that the changes will affect running programs only. After confirming with YES, the program edit window appears (Fig. 64). → ↓ Fig. 65 • Enter the day on which you want the program to end.
5. Operation The RUN OPTIONS function displays the options with which the current program is started. • LEVEL TEST enabled: The lower level sensor in the retort is activated. The program is stopped with an error message if the level is not reached. ↓ • WAVE MOTION activated: During each infiltration step, the reagent is periodically pumped from the bottle to the retort and back to achieve a more evenly mixed blend. The first pump step begins after 16 min, afterwards regularly after 12 min.
5. Operation 5.3.2 Starting a program ↓ Fig. 67 → Fig. 68 • Pressing START starts the program. Confirm the prompt that follows with OK. The number of blocks to be edited (NUMBER OF BLOCKS) must be entered if the RMS has been enabled. • The FINISH TIME button displays the time when the currently running program will end. If events occur that cause the end time to be delayed, the end time display here will be updated accordingly.
5. Operation 5.3.3 Opening the retort The signal tone and the 10-second waiting time occur each time the retort is unlocked! For this reason, we recommend only locking the retort before starting a program or before cleaning in order to prevent unnecessary waiting times. • To unlock, turn the lever for the retort's locking/unlocking mechanism (Fig. 69) clockwise until you feel it engage (Fig. 70) and then release it. A signal tone sounds for about 10 seconds.
5. Operation 5.3.4 Retort emergency release (continued) Aside from this, the system is also equipped with a mechanical emergency unlock (a predetermined breaking point in the retort lock). Mechanical emergency unlock If the retort cannot be opened while in a powered-down state, the stop can be overcome and the retort can be unlocked using the single-head wrench (14 0330 50891) included in the delivery package. Keep the single-head wrench near the instrument! Approx.
5. Operation 5.4 Reagent status → This window (Fig. 64) is used to display and update the current reagent status of the stations. In addition, reagent stations can be marked as full or empty here. The following is displayed: • The current age of each reagent • The current status of each station • Criteria that trigger warning messages (Fields colored in red) To change the status of a reagent, highlight the corresponding row and press the button symbol for the respective action.
5. Operation 5.5 System monitor → → The SYSTEM MONITOR function provides important information about the status of the Leica ASP300 S. PUMP AGE: Indication of the operating hours of the pressure/ vacuum pump. The configured warning value determines the triggering of a warning message. The pump must be maintained by Leica Service after approx. 1000 operating hours. AGE OF THE CARBON FILTER: Displays the operating hours of the active carbon filter.
5. Operation 5.6 The online help The Leica ASP300 S has an extensive help system that can be started from every main window. To obtain information about basic problems, you can use the Help index, which is called up in the Help window using the INDEX key. The help topics are called up in the index according to the hyperlink principle. To scroll in the help window, use the MOVE UP/ MOVE DOWN buttons. The PREVIOUS TOPIC (1) button takes you from a detail back to the index. 2 1 3 Fig.
6. Troubleshooting 6.1 General The Leica ASP300 S is equipped with a cutting-edge fault detection and troubleshooting system. When detecting a malfunction, the instrument provides the operator with detailed error messages and corresponding steps to take via the screen. For all error states, the instrument takes corrective measures to protect the specimens, except in such cases where continued functioning of the instrument would be possible only under hazardous operating conditions.
6. Troubleshooting 6.3 Troubleshooting If a problem occurs in the Leica ASP300 S, take the following steps to diagnose the problem: → • Check for error messages indicating the nature of the problem. • If an error message is displayed, press the Help button on the message for any information relating to the cause. • Check the Run Log for any messages that indicate the nature of the failure.
6. Troubleshooting 6.4 Typical fill or drain problems Fill and drain problems may be due to several causes: 1. There is insufficient reagent • Verify that the fill level of the reagent containers and the paraffin reservoirs is sufficient. 2. Insufficient pressure or vacuum The instrument is unable to create sufficient vacuum (for a fill) or pressure (for a drain) in the retort. • Verify that the reagent container in question is correctly inserted in the reagent cabinet.
7. Cleaning and maintenance 7.1 Clean programs → → The cleaning programs are at the end of the list of all programs (Fig. 43). To display a clean program, highlight the respective line and press EDIT. Three retort cleaning programs and a paraffin cleaning program are defined in the ASP300 S. Clean programs cannot be copied or deleted. Replacement schedule for cleaning reagents: The cleaning reagents (cleaning xylene and cleaning alcohol) must be replaced weekly (refer to Chapter 3.6.1).
7. Cleaning and Maintenance Editing steps • To edit a program step highlight the corresponding line and press the respective table header. • To disable a step, set the number of cycles to "0". Only cleaning water steps can be disabled. The following characteristics of each step can be edited: • Reagent name – only those reagents that are compatible with the reagent station being edited will be displayed. • Number of cycles – the duration displayed is calculated based on the number of cycles.
7. Cleaning and maintenance It is possible to clean used paraffin of solvent residues (xylene) in the Leica ASP300 S. 7.1.2 Paraffin cleaning To do so, select the paraffin bath for which the paraffin should be cleaned in the CLEANING PROGRAMS menu. If the last reagent in the retort is not compatible with paraffin, carry out a retort cleaning first. ↓ ↓ The following steps are taken during the paraffin cleaning: • The paraffin is pumped into the retort.
7. Cleaning and Maintenance 7.1.3 Smart clean → The smart cleaning prepares the ASP300 S for transport. During a smart clean, a series of air cleaning steps will be applied which will remove any excess liquid (condensate) from all internal tubing. Before starting a smart cleaning, a complete retort cleaning program must be carried out in the ASP300 S. To start a smart clean, press the SMART CLEAN button on the MORE MENU FUNCTIONS screen and press OK to confirm. The smart clean itself takes approx.
7. Cleaning and maintenance 7.2 General cleaning steps Paraffin reservoirs Work carefully! Be careful as the walls of the paraffin reservoirs are very hot and may cause burns! • Wipe the paraffin reservoirs and lids clean. The lid can be removed for cleaning purposes. • If they are dirty, remove the wax strainers from the paraffin reservoirs. Clean, dry, and reinsert them. • Ensure that the air vent hole at the top rear right hand corner is unobstructed.
7. Cleaning and Maintenance Draining the condensate container • Remove, empty, and reinsert the blue condensate container (Fig. 83). Dispose of waste solvents with care according to local regulations and the waste management policy of the company or institution. 14 Fig. 83 Replacing the active carbon filter • The life of the active carbon filter will depend on the reagent types used and the frequency of vacuum cycles. • The filter (13) should be replaced at least every 3 months (Fig. 84).
7. Cleaning and maintenance 7.2.1 Daily cleaning and maintenance Cleaning the retort lid • Remove wax from the inside of the retort lid with the plastic scraper provided. Thoroughly remove all wax deposits from around the lid seal. • For convenience, the lid may be removed during cleaning. Lift the lid to a vertical position, release the hinge lock and pull the lid toward you.
7. Cleaning and Maintenance Cleaning the surface of the infiltration module Important in order to ensure that the seals always seal properly. • Remove both covers for cleaning. • First, clean the stainless steel surface using the plastic spatulas, then wipe to remove all dirt particles around the retort and the paraffin reservoirs.
7. Cleaning and maintenance 7.2.2 Periodic cleaning and maintenance Cleaning the reagent containers • Empty and clean the reagent containers. Using a bottle brush and a laboratory detergent in warm water. Never clean reagent containers in an automatic dishwasher. The reagent containers are NOT dishwasher-proof! • Refill and reconnect the bottles once cleaned. Make sure the bottle lids are tight and the bottles are properly seated in their home position at the rear of the reagent module.
7. Cleaning and Maintenance 7.2.2 Periodic cleaning and maintenance (continued) Cleaning the level sensors The level sensors must be cleaned of all residues (reagents and paraffin) after every cleaning program! You will be prompted to clean the sensors by the instrument software at the end of each cleaning program (Fig. 86, 44). 45 44 46 47 Fig. 85 Fig. 86 Fig. 87 Use the cleaning tool together with the microfiber cloth (Fig. 85) as shown in Fig. 87.
7. Cleaning and maintenance 7.3 Checklist for preventive maintenance Step Daily √ Lubricate the O-rings of the reagent container and check them for damage. Monthly every 3 months √* * During remote filling and draining After exiting the retort cleaning program, wipe the retort and cover dry. √ Clean the outer surfaces of the instrument with a soft cloth and a very small amount of xylene. √ Check the retort filter screen for tissue or paraffin residue.
7. Cleaning and Maintenance Step Daily Weekly Check the filter screens of the paraffin reservoirs and clean if necessary. √ Check the air circulation opening of the paraffin reservoirs and clean if necessary. √ Check and empty the condensate container. Clean the inlet openings. √ Monthly √ Check the status of the active carbon filter. √ Replace the active carbon filter. Check the level sensors and clean if necessary. √ Check the electrical ports on the rear side of the instrument.
8. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
Appendix 1 Notes on Specimen Preparation • Cut the tissue samples that are to be embedded to a size that fits the type of tissue and embedding cassette being used. • Use a sharp and clean knife in order to prevent the transmission of contaminants and to avoid damaging the tissue samples. Leica recommends: • Using sponges to affix small tissue samples for processing or packing them in filter paper. • Packing small biopsies into Leica CellSafe biopsy capsules, biopsy sponges or biopsy bags.
Notes 87 Instructions for Use, V 1.
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