User Manual
Table Of Contents
- 1. Important Information
- 1.1 Symbols and their meanings
- 1.2 Qualification of personnel
- 1.3 Intended use of instrument
- 1.4 Instrument type
- 2. Safety
- 2.1 Safety notes
- 2.2 Warnings
- 2.3 Safety features on the instrument
- 3. Instrument Components and Specifications
- 3.1 Overview – instrument components
- 3.2 Specific instrument options
- 3.3 Standard delivery - packing list
- 3.4 Technical Data
- 3.5 Compatible reagents
- 3.6 Recommended reagent handling
- 3.6.1 Cycle for changing reagents
- 4. Instrument Setup
- 4.1 Installation site requirements
- 4.1.1 Moving the instrument
- 4.2 Electrical connection
- 4.2.1 Connecting the power supply
- 4.2.2 Connecting an uninterruptible power supply (UPS)
- 4.3 Installing accessories
- 4.4 Making the data connections
- 4.5 Anti-reflection clip – Function
- 4.6 Alarm functions
- 4.7 Switching the instrument on
- 4.8 Touchscreen functions
- 4.9 Checklist for initial operation
- 4.10 Switching off the instrument
- 5. Operation
- 5.1 Setting up the instrument parameters
- 5.1.1 System setup
- 5.1.2 Access levels
- 5.1.3 INSTALLATION menu
- 5.1.4 Editing the reagent list
- 5.1.5 Viewing the program list
- 5.1.6 Adding and/or modifying programs
- 5.1.7 Favorites
- 5.1.8 Configuring the stations
- 5.1.9 Reagent groups
- 5.2 Reagent handling
- 5.2.1 Draining/filling reagents (other than paraffin)
- 5.2.2 Replacing the paraffin
- 5.3 Running programs
- 5.3.1 Editing a program that has been called up.
- 5.3.2 Starting a program
- 5.3.3 Opening the retort
- 5.3.4 Retort emergency release
- 5.4 Reagent status
- 5.5 System monitor
- 5.6 The online help
- 6. Troubleshooting
- 6.1 General
- 6.2 Power failure
- 6.3 Troubleshooting
- 6.4 Typical fill or drain problems
- 7. Cleaning and maintenance
- 7.1 Clean programs
- 7.1.1 Retort cleaning programs
- 7.1.2 Paraffin cleaning
- 7.1.3 Smart clean
- 7.2 General cleaning steps
- 7.2.1 Daily cleaning and maintenance
- 7.2.2 Periodic cleaning and maintenance
- 7.3 Checklist for preventive maintenance
- 8. Warranty and Service
- Appendix 1
- Notes on Specimen Preparation
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Leica ASP300 S
3. Instrument Components and Specifications
The following reagents may be used in the Leica ASP300 S:
Fixatives Dehydration Clearing Paraffinizing
1st Formalin 1st Ethanol 1st Xylene 1st Wax
(buffered or 2nd Isopropanol 2nd Toluene*
unbuffered) 3. Methanol 3. Chloroform*
2nd Formalin re-
placement
4. Butyl alcohol
5. Industrial methyl-
ated spirits
* Before using these reagents, please obtain information from Leica or the supplier about required
preventive measures.
Reagents other than those listed here may damage some components of the instrument. Do not
use acetone, benzene or trichlorethane in the instrument.
Fixatives containing mercuric salts, acetic or picric acid will corrode metallic components in
the instrument and shorten instrument life.
If you choose to work with such fixatives, it is essential to perform a clean cycle which
contains multiple water rinses each time after use, to minimize damage.
In addition, we recommend frequent and regular preventive maintenance by the Leica Technical
Service.
3.5 Compatible reagents (continued)
3.6 Recommended reagent handling
• The reagents used should be replaced after 1200 to 1800 specimens have been processed or
after 6 cycles of 200 to 300 specimens each.
• For formalin, process alcohol and process xylene reagents, ambient temperature is recom-
mended.
• The recommended temperature for cleaning reagents in the cleaning cycle is 65 °C.
• Only zinc formalin based on zinc sulfates may be used in the ASP200 S/ASP300 S. If zinc formalin
is used, the cleaning program must include an additional cleaning water step.
• The use of formalin reagents containing zinc chloride can cause corrosion in and on the instru-
ment.