Leica CM1100 Cryostat Instructions for use Leica CM1100 V1.3 RevB English – 09/2012 Order-No.
1. Important information Serial No. ..................................................................... Year of manufacture: ................................................. Manufactured: ........ Federal Republic of Germany Until the ratification of the guideline for in-vitro diagnostic instruments this product is categorized according to the MedGV (National Regulations for Medical Appliances) as a Class 3 equipment.
2. Table of contents 1. 2. 3. Important information .................................................................................................................................................... 3 Table of contents ............................................................................................................................................................ 4 Safety instructions for handling the instrument .................................................................................
3. 3.1 Safety features Safety instructions for handling the instrument 3.1.2 Knife guard The instrument design incorporates the following safety features: handwheel lock, glass anti-roll guide of the knife holder CE that has the protective function of a knife guard. 3.1.1 Locking the handwheel Prior to manipulating the knife holder and specimen, or changing the specimen or knife, and during breaks, always lock the handwheel! The handwheel can be locked in the upper turning point.
3. Safety instructions for handling the instrument Transport and installation • The instrument must be transported in an upright position only, or at an angle of max.
3. Sectioning Safety instructions for handling the instrument Removal of the microtome • Take care when handling disposable blades.
4. Technical data Operating temperature range: 18 °C to 35 °C. All specifications related to temperature are valid only for an ambient temperature up to 22 °C and for an air humidity lower than 60%! Type CM 1100-1 CM 1100-3 CM 1100-11 Nominal voltage Nominal frequency Power draw during refrigeration during defrosting for 10 min. Maximum start-up current for 5 sec.
5. 5.1 General description Leica CM 1100 Overview Control unit Cryochamber lid Handwheel with retractable countersunk handle Carrying handles Lever for locking the handwheel Mains switch Fig. 9.1 Compressor ventilation grids Cryochamber Coarse feed wheel with retractable countersunk handle Fig. 9.
5. General description 5.2 Product description The Leica CM1100 is a portable compact cryostat for rapid freezing and manual sectioning of tissue specimens. Two carrying handles on the front and rear of the housing make it easy for two persons to transport the instrument. The counterbalanced handwheel is lockable in the top position of the handle. The corrosion-proof cryochamber is easy to clean and easily accessible after removing the lid.
6. 6.1 Unpacking and installation Unpacking Check the transport crate for external damage on arrival of the shipment. If damages are obvious, please make a complaint to your carrier. 3 • Remove 2 screws (1) at the top and bottom on all of the four sides of the crate (2). 2 • Remove the lid (3). • Lift the body of the crate (2) straight up. 1 1 1 1 • Take the accessory box (4) from the pallet (5). • Remove the foam plate (6). • Remove the inner cardboard box (7) by lifting it upwards.
6. Unpacking and installation 6.2 Site requirements 6.3 Transport to the installation site Do not operate in rooms with explosion hazard! The instrument must be transported in an upright position only, or at an angle of max. 30 °! To ensure an adequate cooling capacity, the instrument has to be set up with at least 10 cm distance from walls and furniture.
7. 7.1 Connection to mains power 7.2 First use of the instrument Installing the accessories • Remove the lid. Please refer to chapter 4 'Technical data'! During the start-up of the compressor the nominal voltage must not drop below the values specified in the 'Technical data'. Please note that the compressor requires a start-up current between 10 and 25 A.
7. First use of the instrument 7.3 Switching on After transporting, wait at least 4 hours before turning the instrument on. This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return to its original position. Failure to comply with this can cause severe damage to the instrument.
7. 7.4.3 Setting user definable parameters • To access parameter programming, press the Pbutton. Any programming field can be accessed for 30 seconds. After that time the display automatically resets to actual temperature indication. Parameters can be modified via the arrow keys. • To adjust the set temperature, press 'P' and modify the value via arrow key. • To set the defrost time, press 'P' again and modify the value via arrow key. First use of the instrument 7.4.
7. First use of the instrument 7.
8. 8.1 Specimen discs Daily operation 8.1.2 Fixing the specimen disc in the specimen head Always clamp the specimen before the blade! 1 Prior to manipulating the knife holder and specimen, or changing the specimen or blade, and during breaks, always lock the handwheel and cover the cutting edge with the anti-roll guide! 3 • Lock the handwheel. • Loosen the screw (3) on the specimen head.
8. Daily operation 8.2 Knife holder CE 8.2.2 Adjusting the rear pressure plate The knife holder CE is equipped with a pressure plate for low profile blades on delivery. A pressure plate for high profile blades is supplied separately. If you wish to use high profile disposable blades, it is necessary to change the rear pressure plate (see 8.2.1). After changing, the rear and front pressure plates need to be readjusted (see 8.2.2 and 8.2.3). 8.2.
8. Daily operation 8.2.4 Inserting the blade 3 Take care when handling disposable blades. The cutting edge is extremely sharp and can cause severe injury! 7 Never leave the knife holder with a blade mounted lying around! 5 Avoid extended skin contact with cold parts of the instrument as this can cause frostbite! All components of the cryostat as well as the knife or blade and the tools for specimen preparation should be precooled in the cryostat before starting sectioning! Fig. 19.1 8.2.
8. Daily operation 8.2.5 Lateral displacement 8.2.6 Adjusting the anti-roll plate If the sectioning results start lacking quality, the knife holder can be displaced laterally to use another blade position. Parallel adjustment of the anti-roll plate and blade It may be necessary to align the front edge of the antiroll plate parallel to cutting edge of the blade. 1 7 10 8 Fig. 20.1 9 Proceed as follows: Fig. 20.
8. • To raise the anti-roll plate (to move it towards the blade), turn the knurled knob (9, Fig. 20.2) counterclockwise. • To lower the anti-roll plate (to retract it from the blade), turn the knurled knob (9, Fig. 20.2) clockwise. Daily operation 8.2.7 Replacing the glass anti-roll plate 3 1 2 If the anti-roll plate is positioned incorrectly to the blade edge, problems I and II will occur. Position III shows the correct position of the anti-roll plate. 6 4 7 5 Fig. 21.
8. Daily operation 8.3 Coarse feed wheel The coarse feed wheel is located on the left side of the housing. It has a retractable countersunk handle. The coarse feed wheel always rotates during sectioning and may therefore not be obstructed. The coarse feed is used for rapid horizontal movement of the specimen, both forward and backward. Clockwise turning moves the sample forward towards the knife. Counterclockwise turning moves the sample backward away from the knife.
8. 8.5 8.6 Sectioning Daily operation Section transfer Take care when removing the section - the cutting edge is exposed! 1 Section transfer to a slide at room temperature • Carefully approach a slide at room temperature to the cut section. The frozen section ‘flies‘ to the slide, rapidly thaws and thus adheres to the slide surface making subsequent orientation impossible. Fig.
8. Daily operation 8.7 Defrosting The Leica CM 1100 provides both programmable automatic and manual defrosting. During a defrost cycle, whether automatic or manual, the evaporator is flushed with hot gas to remove frost that inevitably builds up during routine operation of a cryostat. Such frost formation on the evaporator can result in reduced cooling output. 8.7.1 Programming an automatic defrost cycle An automatic defrost cycle is programmed by entering the defrost time via the control unit (see 7.
9. Trouble shooting Problem Possible causes Remedies Frost on chamber walls and microtome while cryochamber lid not in place - Cryostat is exposed to air currents (open windows and doors, air conditioning). Cryochamber was open. This causes frost formation on the microtome which is much colder than the evaporator. Frost built up by breathing into the cryochamber. - Change place of installation for the cryostat. - - Replace the lid after terminating work. Select higher set temperature (e.g.
9. Trouble shooting Problem Possible causes Remedies Chatter during sectioning - Specimen insufficiently frozen onto the specimen disc. Specimen disc not clamped tightly. Blade not clamped tightly enough. Specimen has been sectioned too thickly and has detached from the disc. Very hard, inhomogeneous specimen. - Refreeze specimen onto the disc. - Check disc clamping. Check clamping. Refreeze specimen onto the disc. - - Blunt cutting edge.
9. Trouble shooting Problem Possible causes Remedies Flattened sections curl up when antiroll plate is picked up - Static electricity or air currents. Anti-roll plate too warm. - Remove static electricity. Cool down the anti-roll plate. Sections tear - Temperature too low for the tissue to be cut. Blunt part, dirt, dust, frost or rust on the blade. Leading edge of anti-roll plate damaged. Hard particles in the tissue. Blade back dirty. - Increase temperature and wait. - Remove cause.
10. Cleaning and disinfection 10.1 Cleaning • Remove frozen section waste from the cold cryostat. • Remove the waste trays and empty. Do not use organic solvents or any other aggressive substances for cleaning and disinfection! We strongly recommend the use of Leica Cryofect disinfectant spray! Only use alcohol or common disinfectants based on alcohol! 10.
10. If increased frost buildup occurs on the evaporator, start a manual defrost cycle. 10.3 Removal of the microtome For extensive cleaning and disinfection, or for thorough drying of the microtome after a long mains power failure, it may be necessary to remove the microtome from the cryochamber. If increased frost buildup occurs on the microtome, the microtome should be removed and thoroughly dried. • Place an appropriate vessel under the drain tube at the rear of the housing.
10. Cleaning and disinfection 10.4 Reassembly of the microtome 1 The microtome must be completely dry before reinstallation. Humidity inside will condense and freeze in the cold cryostat and thus may cause malfunctions or damage. The handle of the handwheel should be placed in the lowest position so that the specimen clamping head is at its lowest position. 2 3 3 Fig. 30.
11. 11.1 General maintenance 11.2 Fuse replacement Only qualified and authorized service personnel may access the internal components of the instrument for service and repair. The microtome is maintenance-free in operation to a great extent! Maintenance Only use a fuse of the same specification! For the required value, please refer to Chapter 4. 'Technical data' or to the fuse specification label at the rear of the instrument.
12. Optional accessories 12.1 Mobile heat extractor 12.2 Thermal block Specimen freezing on the quick-freeze shelf can be accelerated by the additional use of a heat extractor. The thermal block (8) facilitates the removal of the frozen specimen from the specimen disc. Keep the thermal block outside the cryochamber at room temperature. Only place in the cryochamber for specimen removal. Fig. 32.1 5 6 • Store the heat extractor in the cryochamber.
13. Warranty and service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
14. Decontamination Certificate (master) Dear Customer, any product that is to be returned to Leica Microsystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Microsystems for repair.
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Notice 36 Instructions for use V1.
