Leica CM1850 UV Cryostat Instructions for use Leica CM1850 UV, V1.5, English – 10/2012 Order No.
INFORMATION The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Table of contents 1. Important Information ............................................................................................................................ 6 1.1 1.2 1.3 1.4 2. Safety ........................................................................................................................................................ 7 2.1 2.1.1 2.1.2 2.2 2.3 2.4 2.5 2.6 2.7 3. 4. Connection to mains power ....................................................................................
Table of contents 7. Daily use of the instrument ................................................................................................................. 26 7.1 7.1.1 7.1.2 7.2 7.2.1 7.2.2 7.3 7.3.1 7.3.2 7.4 7.5 7.5.1 7.5.2 7.5.3 7.6 7.6.1 7.6.2 8. Troubleshooting .................................................................................................................................. 33 8.1 8.2 8.3 9. Cleaning ..........................................................................
1. Important Information 1.1 Symbols used in this manual and their meaning Warnings appear in a grey box and are marked by a warning triangle . Notes i.e. important user information appears in a grey box and is marked by an information symbol. Caution – UVC radiation! (5) (Fig.5) 1.2 Figures in brackets refer to item numbers in drawings or to the drawings themselves. Qualification of personnel The Leica CM1850 UV may only be operated by trained laboratory personnel.
2. Safety This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument. The instruction manual is an important part of the product which must be read carefully before setting up and using the instrument and must always be kept with the instrument.
2. Safety 2.1.2 Knife guard For every manipulation in the cryochamber, or upon changing a specimen when the knife or disposable blade is clamped, or during breaks, cover the cutting edge with the knife guard. The knife holders CN and CS are equipped with knife guards; on the knife holder CE the glass plate of the anti-roll guide constitutes the knife guard (please refer to the separate instruction manual for your knife holder).
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2. Safety 2.4 Operating the instrument • Be very careful when handling microtome knives and disposable blades.
2. 2.6 Safety Removal of the microtome • Prior to removing the microtome, turn the instrument off with the mains switch and pull the mains plug. • Prior to removing the microtome, bring the specimen head to the lower position with the handwheel. Otherwise the specimen head would rapidly fall down and might injure the operator‘s hands, when taking out the microtome. • Wear appropriate protective gloves to take the cold microtome out of the cryochamber.
3. Technical data Operating temperature range (ambient temperature): 18 °C to 35 °C. All specifications related to temperature are valid only up to an ambient temperature of 22 °C and an air humidity lower than 60 %! Type Mark of conformity Nominal voltage Nominal frequency Power input Max. start-up current for 5 sec Protective class Automatic mains fuse Pollution degree Overvoltage inst. category Heat emission (max.) ☛ ☛ CM1850 UV 100 V AC ±10% 50/60 Hz 1440 VA 45 A eff.
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4. Unpacking and installation 4.1 Site requirements Do not operate in rooms with explosion hazard! To ensure an adequate cooling capacity, the instrument must be set up with at least 10 cm distance from walls and furniture. • The place of installation must meet the following requirements: – no direct sunlight, – mains power socket at a distance no greater than approximately 1.5 m, – no drafts (air condition outlets, etc.
4. Unpacking and installation 4.3 Standard delivery It gives following variations: 1 1 1 1 1 1 1 1 1 1 1 1 1 - CM1850UV with Retraction, in 4 different voltages - CM1850UV without Retraction (only US) ........... 14 0471 31148 knob with marking, antibacterial .................................................................................................. 14 0471 42558 Heat extractor, stationary ...........................................................................................................
4. Unpacking and installation 4.4 Handwheel assembly 1 2 3 4 • Insert the pin (1) of the handwheel shaft in to the hole (2) of the handwheel. 5 • Mount the spring washer (3) on the screw (4) as shown in Fig. 16. • Tighten the screw (4) with an Allen key (5 mm). • Attach the cover disc (5) (self-adhesive). To dismount, proceed in reverse order. Fig. 5 The handwheel including the fixing components are packed in the cardboard box for the accessories.
5. 5.1 Setup Connection to mains power During the start-up of the compressor the nominal voltage must not drop below the values specified in the ‘Technical data‘. Please note that the compressor requires a start-up current between 45 and 50 A. Therefore, the electric circuit at the installation site must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument. A constant adequate power supply to the instrument must be ensured at all times.
5. Setup 5.3 Leica CM1850 UV - Overview 17 15 4 13 16 21 20 8 13 13 19 12 10 18 14 9 Fig. 6 18 11 Instructions for use V 1.
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5. Setup 5.4 Mains switch and automatic mains fuse The automatic mains fuse is used as mains switch. To turn the automatic mains fuse on, the switch must be set in the upper position (pos. 1). To turn the automatic mains fuse off, the switch must be set in the lower position (pos. 0). Fig. 7 Automatic mains fuse 5.5 Turning the instrument on After transporting, wait at least 4 hours before turning the instrument on.
6. Control panel operation 6.1 Control panel 1 Fig. 8 Function keys Lamp button ON/OFF switch for cryochamber illumination. Manual defrost button To activate and deactivate manual defrosting. Key button To lock and unlock the control panel to protect the entered parameters from unintended modifications. To lock or unlock, hold down for approximately 5 seconds.
6. Control panel operation 6.2 Programming the desired values 6.2.1 Setting the time The actual time is set on the panel marked with the clock symbol using the and keys. When pushing the or button for more than 1 second, the time value increases or decreases continuously (autorepeat function). Fig. 9 6.2.2 Setting the automatic defrost time (cryochamber) Fig. 10 The automatic defrost cycle takes place once within 24 hours.
6. Control panel operation 6.2.4 Activation of the Peltier element (option) Fig. 12 The Peltier element is used for cooling the quick-freeze stations. Upon activation of the Peltier element, the compressor of the cooling system is started after 40 seconds to reinforce the thermal conductivity effect. Display reading of instruments without Peltier element: ‘LL‘ with Peltier element: ‘PE‘ The Peltier element is activated by pressing . Once activated, the display indication changes to ‘10‘ (i.e.
6. Control panel operation 6.2.6 Manual defrosting of the cryochamber The manual defrosting of the cryochamber is activated by subsequently pressing the button (audible signal turns on) and the or button on the panel for the cryochamber temperature (audible signal turns off). During the defrost cycle, the indication is flashing. If you want to turn off the manual defrosting of the quick freeze shelf prior to the automatic deactivation, press again and or on the panel for the cryochamber temperature. Fig.
6. Control panel operation 6.4 Control panel 2 - Electric coarse feed Move the specimen away from the knife • Press to start a rapid return travel of the specimen to the rear limit. LED (1) flashes, while the specimen head is in motion. rapid On reaching the rear limit, the LED (1) starts illuminating. 1 • The return movement can be stopped by pressing one of the coarse feed buttons. • Press to start a slow return movement of the specimen to the rear limit.
7. Daily use of the instrument 7.1 Specimen freezing • Select the appropriate sectioning temperature (cryochamber temperature) for the sample material (please refer to 7.4 Temperature selection chart‘). 7.1.1 Quick freeze shelf 1 The cryochamber has a quick freeze shelf (5, Fig. 17) for up to 10 specimen discs. The temperature of the quick freeze shelf is always lower than the cryochamber temperature. 2 • Cut the specimen to size.
7. Daily use of the instrument 7.2 Specimen discs 7.2.1 Inserting the specimen discs in the specimen head • Lock the handle of the handwheel in the upper position. • If the knife holder and a knife are in place, cover the knife edge with the knife guard. • Loosen the screw (1) on the specimen head. • Insert the shaft of the specimen disc (3) in the location hole (2) of the specimen head. Make sure that the shaft of the specimen disc is fully inserted.
7. Daily use of the instrument 7.3 Sectioning All necessary adjustments on the knife holder and anti-roll guide are described in the separate instruction manual for your knife holder in detail. 7.3.1 Trimming the specimen Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause severe injury! • Insert the precooled knife/blade in the knife holder and clamp. • Adjust the appropriate clearance angle on the knife holder.
7. Daily use of the instrument 7.
7. Daily use of the instrument 7.5 Defrosting Defrosting the cryochamber actually means defrosting the evaporator to prevent excessive frost buildup. The evaporator is flushed with hot gas during defrosting. The cryochamber virtually is frostfree and does not need to be defrosted. The condensation water that is produced during defrosting is collected in a container, which is located at the front of the cryostat cabinet.
7. Daily use of the instrument 7.5.3 Manual defrosting of the quick freeze shelf The quick freeze shelf may become hot during defrosting! Therefore, do not touch it! If increased frost formation occurs on the quick freeze shelf, especially after spray disinfection, a manual defrost cycle should be started (see 6.2.5), which can be terminated when required. 7.6 Terminating work 7.6.1 Terminating daily work • Lock the handwheel.
7. Daily use of the instrument 7.6.2 Shutdown for a longer period If you do not intend to use the instrument for several weeks you may turn it off. Please note, however, that it may take up to several hours to cool the cryochamber down to very low temperatures after turning the instrument on again. After turning off, the instrument should be cleaned and disinfected thoroughly (see chapter 9 ‘Cleaning, disinfection and maintenance'). • • • • • Turn the instrument off with the automatic mains fuse.
8. Troubleshooting 8.1 Error messages in the display Error messages are displayed on the clock panel as follows: EO: XX. The following error messages might occur during operation: Fig. 20 Error Description Remedy 20 Calibrating error; possibly defective controller board. 21 22 23 Clock battery on the controller board empty. Microtome wet. Cryochamber temperature out of range of indication. (from -35 °C to +55 °C) Short circuit at the temperature sensor of the chamber cooling system.
8. Troubleshooting 8.3 Possible causes and remedies Problem Cause Remedy Frost on chamber walls and microtome - Cryostat is exposed to air currents (open windows and doors, air conditioning). - Sliding window was open and exposed to air currents too long. - Frost built up by breathing into the cryochamber. - Change place of installation for the cryostat. Ice formation on the bottom of the cryochamber - Condensation water drain obstructed.
8. Troubleshooting Problem Cause Remedy Sections not properly flattened despite correct temperature and correctly aligned anti-roll plate - Knife/blade and/or anti-roll plate dirty. - Edge of anti-roll pate damaged. - Blunt knife/blade. - Clean with dry cloth or brush. Sections curl on the anti-roll plate - Anti-roll plate does not protrude far enough beyond the cutting edge. - Readjust correctly.
8. Troubleshooting Problem Cause Remedy - Knife profile inappropriate for the specimen to be cut. - Incorrect clearance angle. - Use knife with different profile. Condensation on anti-roll plate and knife during cleaning - Brush, forceps and/or cloth are too warm. - Store all tools on shelf in the chamber. Anti-roll plate damage after adjustment - Plate too high above the cutting edge. The adjustment was carried out in the direction of the cutting edge. - Raise plate when aligning.
8. Troubleshooting Problem Cause Remedy Fat on the corner or edge of the anti-roll plate. - Rusty knife/blade. - Remove fat with alcohol. - Static electricity or air currents. - Anti-roll plate too warm. - Remove static electricity. - Temperature too low for the tissue cut. - Blunt part, dirt, dust, frost or rust on the knife/blade. - Leading edge of anti-roll plate damaged. - Hard particles in the tissue. - Knife back dirty. - Increase temperature and wait. - Remove cause.
8. Troubleshooting Problem Cause Remedy No or insufficient refrigeration - Compressor defective. - Leak in the cooling system. - Inappropriate site conditions. - Call technical service. - Call technical service. - Check site conditions as described in section 5.2. - Clean the ventilation slits as described in section 10.3.1. - Ventilation grilles of the condenser dirty. Scraping noise at the slot cover of the microtome - Friction between the slot cover and the microtome housing.
9. Cleaning, disinfection, maintenance 9.1 Cleaning • Remove frozen section waste from the cryostat with a cold brush every day. • Remove the section waste tray for emptying. • Remove the storage shelves and the brush shelf for cleaning. • Remove the sliding window by slightly lifting and pulling it to the front when closed (see 9.3.6 'Replacement of the lamp').
9. Cleaning, disinfection, maintenance 9.2 Spray disinfection with Leica Cryofect Basically, we recommend UV disinfection (see page 21 for detailed instructions). For easy-to-use spray disinfection we recommend Leica Cryofect. (Cryofect is not available in all countries!) The cryostat has to be disinfected after each daily use. Comply with the instructions for use! The glass anti-roll plate can remain in place during disinfection. 1. Select a cryochamber temperature value down to -20 °C. 2.
9. Cleaning, disinfection, maintenance 9.3 Maintenance 9.3.1 General maintenance 1 The microtome is virtually maintenance-free. To ensure a smooth operation of the instrument over several years we recommend the following: • Have the instrument inspected by a qualified service engineer authorized by us once a year. 4 2 • Enter into a service contract at the end of the warranty period. For further information, please contact your local Leica service center. 3 • Clean the instrument every day. Fig.
9. Cleaning, disinfection, maintenance the lowest position and apply some drops of cryostat oil on to the slot cover; distribute the applied oil by turning the handwheel or with a soft tissue. • Clean the ventilation slits (5) of the liquefier on the right side of the instrument with a brush, broom or vacuum cleaner from dust and dirt in the direction of the fins. • Do not carry out any repairs on your own as this will invalidate the warranty.
9. Cleaning, disinfection, maintenance 2 4 • Loosen the screws (3) with an Allen key (4 mm). Do not loosen the screws (7) for removing the microtome. • Disconnect the coarse feed motor plug (1) by pulling the metal head. 3 • Disconnect the temperature sensor (2) from the microtome. 1 8 8 Fig. 25 7 • Slightly lift the microtome and pull it to the left to disengage the plastic coupling (5) connecting the axes. 5 • Take the microtome out of the cryochamber. Fig.
9. Cleaning, disinfection, maintenance 9.3.3 Removal of the microtome cover The microtome cover may be removed to expedite thorough drying of the microtome in an oven. Note: Place the microtome in an oven at 40 °C to 50 °C for several hours. After repeatedly drying the microtome in this manner, it may become necessary to relubricate the cross roller bearings! For further information, please contact your sales company! 8 8 8 8 Fig. 27 • Loosen the two screws (8) on both sides of the cover.
9. Cleaning, disinfection, maintenance Ensure that the microtome is completely dry before reinstallation. Humidity inside will condense and freeze and thus cause malfunctions or damage to the feed system of the microtome. • Use your right hand to bring the handwheel handle in the lowest position and keep the handle in place. The specimen head remains in the lower position.
9. Cleaning, disinfection, maintenance 9.3.5 Replacement of the fuses Turn the instrument off with the automatic mains fuse and pull the mains plug, before replacing the fuses! Only use fuses of the same specification! For the required values, please refer Chapter 3 ‘Technical Data‘. The use of fuses other than specified by the manufacturer may cause severe damage to the instrument! On the back of the instrument there is a fuse box with 5 fuses: Fig.
9. Cleaning, disinfection, maintenance 4 3 5 Fig. 31 6 7 8 Fig. 32 Fig. 33 Removal of the lamp The UVC lamp (3) is installed in front of the protection screen for chamber illumination (4). • Hold the lamp with both hands and carefully pull it out of the clips (5) with a slight forward movement. • Detach the metal ring (7) on the holder (6) in direction of the arrow (8) and carefully pull the lamp out of the holder with your right hand (see Fig. 32).
9. Cleaning, disinfection, maintenance • Turn the instrument off with the automatic mains fuse. • Pull the mains plug. • Slightly lift the sliding window (1) holding it by the grip (2) and pull it out to the front. • For the technical specification of the lamp, please refer to Chapter 4. ‘Technical data‘. 9.3.7 Replacement of the lamp 1 2 Fig. 34 Removal of the lamp 4 Fig. 35 5 3 6 The lamp (3) is mounted behind a glare shield (4) and therefore not visible. • Touch the lamp for better orientation.
10. Ordering information, optional accessories 10.1 Ordering information Knife holder base .............................................................................................................. 14 0419 26140 Knife holder CN ................................................................................................................. 14 0419 33993 Knife support ......................................................................................................................
10. Ordering information, optional accessories Antistatic kit for knife holder CE, low profile ................................................................. 14 0800 37739 Antistatic kit for knife holder CE, high profile ............................................................... 14 0800 37740 Cryoembedding system, complete set ........................................................................... 14 0201 39115 Embedding well bar set, 18 mm ......................................................
10. Ordering information, optional accessories 10.2 Optional accessories 10.2.1 Mobile heat extractor Specimen freezing with the freezing shelf can be accelerated by the additional use of a heat extractor. • Store the heat extractor in the cryochamber. • Place it on the specimen surface to accelerate freezing. • Remove it once the specimen is entirely frozen. It is recommended to precool the heat extractor in liquid nitrogen or other refrigerant. Fig.
11. Warranty and service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
12. Decontamination Certificate (master) Dear Customer, any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems for repair.
12. Decontamination Certificate (master) 3 Yes No The equipment has been prepared to ensure safe handling/transportation. Whenever possible, please use the original transportation case/box. Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging or hand it over to the service engineer. Customer assumes all responsibility for the immediate return shipment of articles sent to Leica without proper decontamination documentation.
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Notice 56 Instructions for use V 1.
