Instruction Manual

5
Leica CM1850 UV – Cryostat
Table of contents
7. Daily use of the instrument ................................................................................................................. 26
7.1 Specimen freezing ............................................................................................................................................... 26
7.1.1 Quick freeze shelf ................................................................................................................................................ 26
7.1.2 Stationary heat extractor ................................................................................................................................... 26
7.2 Specimen discs ................................................................................................................................................... 27
7.2.1 Inserting the specimen discs in the specimen head ..................................................................................... 27
7.2.2 Specimen orientation.......................................................................................................................................... 27
7.3 Sectioning ............................................................................................................................................................. 28
7.3.1 Trimming the specimen ...................................................................................................................................... 28
7.3.2 Section thickness setting ................................................................................................................................... 28
7.4 Temperature selection chart (in minus °C) .................................................................................................... 29
7.5 Defrosting ............................................................................................................................................................. 30
7.5.1 Automatic defrosting of the cryochamber ...................................................................................................... 30
7.5.2 Manual defrosting of the cryochamber ........................................................................................................... 30
7.5.3 Manual defrosting of the quick freeze shelf ................................................................................................... 31
7.6 Terminating work ................................................................................................................................................. 31
7.6.1 Terminating daily work ....................................................................................................................................... 31
7.6.2 Shutdown for a longer period ............................................................................................................................ 32
8. Troubleshooting.................................................................................................................................. 33
8.1 Error messages in the display ....................................................................................................................... 33
8.2 Temperature control button .......................................................................................................................... 33
8.3 Possible causes and remedies..................................................................................................................... 34
9. Cleaning, disinfection, maintenance ............................................................................................ 39
9.1 Cleaning ............................................................................................................................................................... 39
9.2 Spray disinfection with Leica Cryofect ...................................................................................................... 40
9.3 Maintenance...................................................................................................................................................... 41
9.3.1 General maintenance ...................................................................................................................................... 41
9.3.2 Removal of the microtome .............................................................................................................................. 42
9.3.3 Removal of the microtome cover.................................................................................................................. 44
9.3.4 Reinstallation of the microtome ................................................................................................................... 44
9.3.6 Replacement of UVC lamp .............................................................................................................................. 46
9.3.5 Replacement of the fuses .............................................................................................................................. 46
9.3.7 Replacement of the lamp ............................................................................................................................... 48
10. Ordering information, optional accessories ............................................................................... 49
10.1 Ordering information ........................................................................................................................................ 49
10.2 Optional accessories....................................................................................................................................... 51
10.2.1 Mobile heat extractor...................................................................................................................................... 51
10.2.2 Thermal block..................................................................................................................................................... 51
11. Warranty and service ........................................................................................................................ 52
12. Decontamination Certificate (master) ........................................................................................... 53
13. Information for the People´s Republik of China .......................................................................... 55