Owner manual
Table Of Contents
- 1. Important Information
- 1.1 Symbols in the text and their meanings
- 1.2 Qualification of personnel
- 1.3 Intended use of instrument
- 1.4 Instrument type
- 2. Safety
- 2.1 Safety notes
- 2.2 Warnings
- 2.3 Safety devices
- 2.3.1 Locking/unlocking the handwheel
- 2.3.2 Knife guard
- 2.4 Operating conditions
- 2.5 Operating the instrument
- 2.6 Cleaning and disinfection
- 2.7 Removing the microtome
- 2.8 Maintenance
- 3. Technical Data
- 4. Setting up the Instrument
- 4.1 Installation site requirements
- 4.2 Unpacking instructions for the cryostat cm1860/1860UV
- 4.3 Standard delivery—packing list
- 4.4 Assembling the handwheel
- 5. Instrument Setup
- 5.1 Electrical connection
- 5.2 Prior to operation
- 5.3 Overview of the Leica cm1860/cm1860UV
- 5.4 Power switch and circuit breaker
- 5.5 Switching on the instrument
- 6. Operating the Instrument
- 6.1 Control panel 1
- 6.2 Programming the desired values
- 6.2.1 Setting the time
- 6.2.2 Setting the automatic defrost time (cryochamber)
- 6.2.3 Selecting the cryochamber temperature
- 6.2.4 Activation of the Peltier element (option)
- 6.2.5 Manual defrosting of the quick freeze shelf
- 6.2.6 Manual defrosting of the cryochamber
- 6.2.7 Setting the section thickness
- 6.3 Retraction (factory default setting: disabled)
- 6.4 Display lock
- 6.5 Control panel 2 - Electric coarse feed
- 7. Daily Use of the Instrument
- 7.1 Specimen freezing
- 7.1.1 Quick freeze shelf
- 7.1.2 Stationary heat extractor (optional)
- 7.2 Specimen discs
- 7.2.2 Specimen orientation
- 7.2.1 Inserting the specimen discs into the specimen head
- 7.3 Sectioning
- 7.3.1 Blade holder CE
- 7.3.2 Blade holder CE-TC
- 7.3.3 Knife holder CN
- 7.3.4 Knife holder CN-Z with anti-roll guide
- 7.3.5 Cleaning the blade and knife holders
- 7.3.6 Installing the shelf (movable) (optional)
- 7.4 Temperature selection chart (in minus °C)
- 7.5 Defrosting
- 7.5.1 Automatic defrosting of the cryochamber
- 7.5.2 Manual defrosting of the cryochamber
- 7.5.3 Manual defrosting of the quick freeze shelf
- 7.6 Terminating work
- 7.6.1 Terminating daily work
- 7.6.2 Shutdown for a longer period
- 8. Troubleshooting
- 8.1 Error messages in the display
- 8.2 Temperature control button
- 8.3 Possible sources of error, causes and remedies
- 8.3.1 Changing the battery
- 9. Cleaning, Disinfection, Maintenance
- 9.1 Cleaning
- 9.2 Spray disinfection with Leica Cryofect
- 9.3 Maintenance
- 9.3.1 General maintenance instructions
- 9.3.2 Replacing the fuses
- 9.3.3 Replacing the UVC lamp
- 9.3.4 Replacing the fluorescent lamp
- 10. Ordering Information, Components and Consumables
- 10.1 Ordering information
- 10.2 Mobile heat extractor – application
- 10.3 Cold extractor – application
- 11. Warranty and Service
- 12. Decontamination Certificate (Master)
78
Instructions for Use V 1.4 – 10/2012
Fig. 84
Cryoembedding system, complete
consisting of:
- 3 embedding well bars with recesses,
in three sizes: 18 mm, 24 mm, 30 mm
- 6 specimen stages, small
- 4 specimen stages, large
- 4 heat extractors
- 1 container for specimen stages
- 16 dispensing slides
- 1 sectioning board/freezing griddle
- 1 heat extractor, elevated
- 1 embedding forceps, angled
Order No. ........................................... 14 0201 39115
Fig. 85
Embedding well bar set with small recesses
consisting of:
- 1 embedding well bar, recesses 18 mm
- 4 specimen stages, small
- 2 heat extractors
- 8 dispensing slides
Order No. ........................................... 14 0201 39116
Embedding well bar set with medium-sized re-
cesses consisting of:
- 1 embedding well bar, recesses 24 mm
- 4 specimen stages, small
- 2 heat extractors
- 8 dispensing slides
Order No. ........................................... 14 0201 39117
Embedding well bar set with large recesses
consisting of:
- 1 embedding well bar, recesses 30 mm
- 4 specimen stages, large
- 2 heat extractors
- 8 dispensing slides
Order No. ........................................... 14 0201 39118
10. Ordering Information, Components and Consumables