Leica CM3600 XP Cryomacrotome Instructions for use Leica CM3600 XP, 6v3, English - 01/2013 Order-No.: 14 0417 80101 RevB Always keep this manual with the instrument. Read carefully before working with the instrument.
NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Table of contents 1. Important Information ........................................................................................................ 8 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Symbols in the text and their meanings .......................................................................................................................... 8 Qualification of personnel ..............................................................................................................................................
Table of contents 6. Software ............................................................................................................................. 34 6.1 Start and log-in procedure ............................................................................................................................................... 34 6.2 Description of the window elements ............................................................................................................................. 35 6.2.
Table of contents 6.15 Saving ................................................................................................................................................................................... 86 6.16 Printing labels ..................................................................................................................................................................... 86 6.17 Foot switch ............................................................................................
Leica CM3600 XP – Cryomacrotome 7
1. Important Information 1.1 Symbols in the text and their meanings Warnings appear in a gray box and are marked by a warning triangle. Useful tips, i.e. important information for the user, appear in gray boxes and are marked by an information symbol. 1.3 Instrument type All information contained in this Instructions for use applies exclusively to the instrument type indicated on the title page. Caution! This Instructions for use is valid ONLY for the instrument with Prod. No. 0163/08.
1. 1.4 Important Information Designated use The Leica CM3600 XP is a PLC-controlled and motorized precision cryomacrotome for large-surface sections, equipped with a convectioncooled cryochamber for deep-freezing, sectioning, storing and freezedrying large biological and industrial specimens. The instrument is designed for the above described applications only and must be operated according to the instructions in the present Instructions for use.
1. Important Information 1.5 Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
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2. Characteristics of the Leica CM3600 XP 2.
2. Characteristics of the Leica CM3600 XP Drain valve Condensation water is drained through the drain valve. The tray at the drain valve must remain at the instrument at all times and must be emptied regularly. Refrigeration and control unit The refrigeration and control unit cools the cryochamber and controls the microtome and the entire electronics. Knee lever The knee lever is used for manual operation of the specimen sledge. PC Commercial PC, monitor, keyboard, mouse and internal modem.
2. Characteristics of the Leica CM3600 XP 2.2 Technical Data 2.2.1 Microtome type 450 C Sliding microtome Section thickness selection: Sectioning range: Max. sectioning speed: Knife retraction after sectioning stroke: Total vertical stroke of knife: Total vertical stroke of knife: Total horizontal stroke: Specimen orientation via ball-joint: Specimen orientation w/large spec. stage 1 - 200 μm 0 - 450 mm 80 mm/s 50 - 250 μm 70 mm (standard) 100 mm (version with extended vertical stroke) 450 mm ca.
2. Characteristics of the Leica CM3600 XP 2.2.3 Dimensions and weight Inner (cryochamber L x H x W): Outer (L x H x W): Total dimensions with connecting lines: Outer length (total): Outer length (chamber only): Outer length (control unit only): Outer height (total): Outer height (work surface height): Outer width: Weight (total, incl. microtome): Weight (microtome only): 1650 x 720 x 600 mm 2800 x 1090 x 920 mm 2800 mm 1940 mm 860 mm 1090 mm 1050 mm 920 mm 660 kg 190 kg 2.2.
2. Characteristics of the Leica CM3600 XP 200 V / 60 Hz, two-phase Nominal voltage: Nominal frequency: Line voltage tolerance: Power draw: Automatic cutout/ Power safety switch 200 V AC 60 Hz ± 10 % 2400 VA 2 x 8A / 1 x 16A UL-listed 220 V / 60 Hz, two-phase Nominal voltage: Nominal frequency: Line voltage tolerance: Power draw: Automatic cutout/ Power safety switch 220 V AC 60 Hz ± 10 % 2400 VA 2 x 8A / 1 x 16A UL-listed 2.2.
2. Characteristics of the Leica CM3600 XP 2.2.6 General parameters Protective class: Pollution degree: Overvoltage installation category: I 2 II A-weighted noise level: Distance between sound meter and instrument: Measuring uncertainty: Instrument settings at measurement: 78.5 dB (A) 1 meter ±1.5 dB (A) Normal instrument operation with extraction Noise information The user must wear hearing protection when using the extraction. 2.
2. Characteristics of the Leica CM3600 XP 2.4 Function The motor-driven specimen sledge moves horizontally underneath the knife, either producing a section (cryosectioning) with each stroke or a high-quality specimen surface (cryo-planing technique). Prior to each sectioning stroke, a stepper motor feeds the microtome knife downwards towards the specimen by a selected amount of microns (section thickness).
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3. Safety The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. 3.1 Safety notes This Instructions for use contains important instructions and information regarding the operational safety and maintenance of the instrument.
3. Safety The protective devices on the instrument must neither be removed nor modified. Only connect the instrument to a grounded power socket. Do not interfere with the grounding function by using an extension cord without a ground wire. Use extreme caution when handling microtome knives and disposable blades. Handle knives and blades as per manufacturer's directions. Never operate the instrument in rooms with explosion hazard.
3. Safety Below you find a summary of all warnings contained in this Instructions for use. Any use of the instrument not described in this Instructions for use is considered improper. Always swing the knee lever upwards before leaving the instrument. The chamber lid must be lifted by 2 persons when opened. When opening the chamber lid, the lid retainer must engage. Only when the lid is lifted can the user work in the instrument.
3. Safety Prior to any work involving the knife, microtome or inside the cryochamber, swing the knee lever upwards. For any work involving the knife, microtome or inside the cabinet always wear special protective gloves. For reasons of safety and accuracy, the maximum trimming thickness should not exceed 100 μm per section. When trimming biological material (particularly bone), section thickness should not exceed 50 μm. Never try to catch or grab the knife.
3. Safety 3.3 Safety devices 3.3.1 Emergency stop switch The Leica CM3600 XP is equipped with an emergency stop switch (1) located on the chamber surface to the left of the window. Activating the emergency stop switch Press the Emergency stop switch (1) to stop the microtome immediately. Always press the emergency stop switch before making modifications to the microtome, knife or specimen (see warning message below the disc) to prevent accidental activation of the microtome using the knee lever.
3. Safety 3.3.2 Knee lever Never leave the instrument unattended when the knee lever is folded out (1) to prevent the microtome from being set in motion accidentally. 1 Always swing the knee lever upwards before leaving the instrument. 3.3.3 Window The window is heatable and equipped with a lock button (2). To open the window, lift lock button (2) slightly upwards and turn it by 180°. 2 3.3.
4. Site requirements 4.1 Site requirements at place of installation • • • • Relative humidity not over 60 % and non-condensing. Room temperature consistently between +5 °C and +22 °C. Room volume must be at least 30 m3. The substrate must have sufficient load capacity and rigidity for the weight of the instrument, 660 kg. • Do not operate the instrument in rooms with explosion hazard. If possible at all, the room should be air-conditioned.
4. 4.2 Site requirements Electrical connections Country: Installation cord cross section: Fuse/connection: Europe 2.5 mm2 16 A/380 V USA 2.5 mm2 25 A/208 V Japan 2.5 mm2 25 A/200 V Power cable length is 3 m Leakage current > 3.5 mA A second protective conductor terminal is located on the instrument. Both terminals must be used. There must not be any differences in potential between the two protective conductor terminals. 4.
5. Installation 5.1 Unpacking and installation Only duly trained personnel may unpack and install the Leica CM3600 XP. Please contact your local Leica sales organization for appropriate advice. 5.
5. 5.3 Installation Port and switch panel Port and switch panel Interface RJ 45 Connection for foot switch Connection for compressed air GFCI switch Switch for local alarm signal Connection for external alarm Control lamps Circuit breaker Main switch Caution! Only the POWER SWITCH disconnects the instrument from the power supply! To do so, move the switch into the "0" position.
5. Installation 5.3.1 Port and switch functions Main switch In the "0" position, this switch disconnects the instrument from the power supply. Connection for footswitch Optionally, a foot switch can be connected for the purpose of section thickness documentation (GLP). RS 232 interface The RS 232 interface links the PC to the PLC. Switch for local alarm signal To activate the local alarm function, press the alarm signal switch. The green LED in the switch button lights up.
5. Installation Connection for external alarm Forwarding an external alarm. The external alarm is triggered, when the chamber temperature exceeds the selected value. When the temperature dips back down below the defined threshold, the external alarm is immediately switched off. Loss of mains power supply (instrument switched off or power failure) will also trigger the external alarm. On return of mains supply the alarm is automatically switched off.
5. Installation GFCI switch Protects the entire instrument. Automatic cutout Protects the refrigerating sets and all electronic components. 5.3.2 Fuses The Leica CM3600 XP is equipped with the following fuses: • GFCI switch • Automatic cutout • Fuse protecting the main power switch GFCI switch After the GFCI switch has been activated, it will be in the lower position. To switch it back on, flip the GFCI switch upwards. To check the function, trigger the GFCI switch once a month.
5. Installation Fuse protecting the mains switch Fuse for power switch 5.4 If the mains switch fuse has blown, please call Technical Service. PC The Leica CM3600 XP is equipped with a commercial PC, monitor, keyboard, mouse and an internal modem. For further information, please refer to the manual of the PC.
6. Software 6.1 Start and log-in procedure Requirements: PC must be on and the Microsoft Windows XP® operating system must have booted and be ready for service. Doubleclicking on Icon "CM3600 XP" starts the application. Registration mask 1. Type the user name in the identification field. 2. Type the correct password in the password field. 3. Click the OK button to complete registration. After successful registration, the "Main window" will open. For further information, refer to chapter 6.4.
6. 6.2 Software Description of the window elements Title bar Tool bar Work space Status bar The window surface is divided into several areas: • • • • Title bar Tool bar Work space Status bar 6.2.1 Title bar The "Title bar" displays information on instrument name and software version number. 6.2.2 Tool bar The "Tool bar" provides quick access (via mouse-click) to individual software functions. When clicking on an icon, the corresponding window will open up. Active icons will light up in green.
6. Software Icons Clicking on the icon opens the "Main window", which also opens up after completion of the start and log-in procedure. Clicking on the icon opens the "Parameter settings" window. Clicking on the icon opens the "Temperature curve" window. Clicking on the icon opens the "Chronological event list" window. Clicking on the icon opens the "Alarm list" window. Clicking on the icon switches the chamber illumination on or off. Active icons will light up in yellow.
6. Software Clicking on the icon opens the "Label printing" window. When activated, the icon will light up in green. Clicking on the icon activates the "Foot switch". When activated, the icon will light up in green and the current sectioning data will be saved. The "Stop" icon appears immediately when the emergency stop switch is pressed - it will be blinking in red color. The instrument will be inoperational until the operator acknowledges the corresponding error message in the "Alarm list" window.
6. Software 6.3 Initialization To be able to work with the instrument, after completion of the start and log-in procedure the microtome must be initialized. For initialization, proceed as follows: 1. In the "Main window", click on the INIT button blinking in yellow color (in "Mode" field). The "Safety check - initialization" window will open up. 2. Check whether you can confirm each safety query - if not, carry out the required actions.
6. Software Once initialization has been started, it can only be interrupted by pressing the emergency stop switch. For further information, refer to chapter 3.4.1. After the initialization procedure has been completed, the color of the INIT button will first change from yellow to green and after a few seconds, the caption on the button will change from INIT to AUTOMATIC. The microtome is now ready for service. For further information, refer to chapter 6.4.9.
6. Software 6.4 Main window The main window either opens up automatically after completion of the start and log-in procedure or by clicking on the "Main window" icon.
6. Software 6.4.1 Chamber temperature Indication of set or actual chamber temperature. See chapter 6.5.1 "Parameter settings" on how to configure these settings. 6.4.2 Time Indication of real time and date. See chapter 6.5.1 "Parameter settings" on how to set the chamber temperature. 6.4.3 Automatic defrost cycle Indication of start time of 1st or 2nd automatic defrost cycle. See chapter 6.5.1 "Parameter settings" on how to configure these settings.
6. Software 6.4.4 Automatic dehydration Indication of start time, start date and duration of the automatic dehydration. See chapter 6.5.1 "Parameter settings" on how to configure these settings. MANUAL DEHYDRATION button Clicking on this button immediately starts a dehydration cycle. Clicking on this button once more, stops dehydration. When activated, the button blinks in yellow color.
6. Software The red "Retraction" caption is displayed only during the return stroke of the sledge. The "Knife movement" icons can be activated only in MANUAL Mode. For further information, refer to chapter 6.4.9. The entry limit for actual and set position may vary between 70 - 200 μm depending on the instrument version. The standard version is shipped with a limit of 70 μm. To modify any of the settings, mouse-click on the corresponding icon. Entries are completed by pressing the ENTER key.
6. Software KNIFE DOWN button on the right Clicking on this button moves the knife holder fast downwards for a short while. If you click on and hold this button, the knife holder will continue to move downward until the left mouse button is released. While active, the button lights up in yellow. PRESELECTION button Clicking on this button activates the "Set position" display. Click on the displayed value to change the setting. While active, the icon lights up in green color.
6. Software 6.4.6 Extraction system The extraction is active only if an extraction device is connected to the instrument. For further information, refer to chapter 9.1. Button EXTRACTION ON/OFF in continuous mode Clicking on this button activates or deactivates continuous extraction. When activated, the button will light up in green. Button EXTRACTION ON/OFF in intermittent mode Clicking on this button activates or deactivates intermittent extraction. When activated, the button will light up in green.
6. Software Click on the activated set value field to modify the setting. PAUSE button Clicking on this button stops the sectioning program. Clicking on the button once more restarts the sectioning program, resuming the current section where if was left off. 6.4.8 Sledge speed Indication of current sectioning or return stroke speed in mm/s (millimeters per second). To modify any of the settings, click on the corresponding value. The graphic display is active only while sectioning is in progress.
6. Software 6.4.9 Mode of operation To be able to work with the instrument, after completion of the start and log-in procedure the microtome must be initialized. For further information, refer to chapter 6.3. Buttons prior to initialization Buttons after initialization INIT button Clicking on INIT opens the "Initialization" window. For further information, refer to chapter 6.3. Prior to initialization, the button blinks in yellow color.
6. Software 6.4.10 Cutting window Indication of the left and right block edge, graphically displayed by blue lines. They indicate the maximum horizontal stroke of the microtome sledge on a scale from 0 - 500 mm. Indication of the actual position of the microtome sledge. The sledge position is graphically displayed by the green line. To modify any of the settings, mouse-click on the "Actual position" indication.
6. 6.5 Software Parameter settings Click on "Parameter settings" to open the corresponding window.
6. Software 6.5.1 Parameter setting Set chamber temperature Click on this indication to change the currently selected value. Temperature values from -30 to +1 °C can be selected. Alarm temperature Click on this indication to change the currently selected value. Temperature values from -25 to -5 °C can be selected. The alarm temperature should not be too close to the set chamber temperature.
6. Software Time and date Indication of time and date. Clicking on this indication opens the dialog box "System time". Clicking on individual parameters (day, month, year, hour or minutes) the corresponding setting can be changed. Click OK to confirm. Start time 1 defrost Indication of start time of the 1st automatic defrost cycle. Clicking on this indication opens the dialog box "defrost time 1". Click on individual parameters (hours or minutes) to change the corresponding settings.
6. Software Dehydration date Dehydration start time Dehydration duration Indication of start date and time, and duration of the automatic dehydration cycle. Clicking on one of the parameters opens the dialog box "Dehydration time". Click on individual parameters (day, month, year, hour or minutes) to change the corresponding settings. The desired duration of the dehydration procedure can be selected in the duration field. Values from 1 - 48 h (hours) can be selected. Click OK to confirm.
6. Software Cutting speed Return speed Indication of cutting and return speed. Click on one of the indications to change the currently selected value. Values from 0 - 80 mm/s can be selected. Left block edge Right block edge Indication of left and right block edge. Click on the individual indication to change the currently selected value. Values from 0 - 500 mm/s can be selected. For further information, refer to chapter 6.4.10.
6. Software 6.5.2 Password list To access the "User configuration", the registered user must be an administrator. The "user configuration" indication consists of the following columns: • No. Indication of the program-controlled running number. • Active Indication of status of the user selected. Active users are displayed with an "X". • User name Indication of the user name, which is introduced in the identification field during the start and log-in procedure. • Full name Indication of full user name.
6. Software CLOSE button Clicking on this button closes the "User configuration" window. Doubleclicking on a line in the configuration indication opens the dialog box "User configuration". In the "user configuration" dialog box the following settings can be selected: • No. Indication of the running number. No changes can be made in this field. • Active Clicking on the check box sets the user status to "active". Only after this step can a user successfully register via the start and log-in procedure.
6. Software • User rights Click on the USER RIGHTS line to open a drop down menu. User property "Administrator" or "User" can be selected. Administrators have full access to all application functions. Users do not have access to the password list and to the configuration parameters in the "Parameter settings" window. • Identification Type in the identification which is needed for the start and log-in procedure. • Confirm identification Repeat the identification.
6. Software 6.5.3 Configuration To access "Configuration", the registered user must be an administrator. The "Configuration" window contains the following tabs: • • • • • • Text main study Text substudy Measured values General Labels Comments OK button Clicking on this button saves the entries and closes the "Configuration" dialog box. CANCEL button Clicking on this button closes the "Configuration" window without saving any of the changes made.
6. Software Tabs "Text main study" and "Text substudy" The structure of the two tabs is identical. A header and 7 freely selectable comments for each main and/or substudy can be defined. The text entered into the 8 entry fields is copied into the dialog box for creating main studies and substudies. Each individual text can consist of up to 30 characters. For the configuration of a main study or substudy see chapter 6.10.
6. Software For the configuration of a main study or substudy see chapter 6.10.
6. Software Company/lab Enter the name of the company or the laboratory. The name will be displayed in the "information bar", on the right side, see chapter 6.2.4. Printer for label printing Clicking on this line opens a drop down menu. If a printer for label printing is connected, it can be selected in this menu. Font size Clicking on this line opens a drop down menu. Here you can select the desired font size for the labels. Printer for protocoling Clicking on this line opens a drop down menu.
6. Software The export file is filed as a text file and according to the export path selected. Millitron Provided an external Millitron measuring device is connected to the PC, the corresponding serial connecting port can be selected via the drop down menu. Activating the millitron Clicking on the check box activates the external millitron measuring device. "Labels" tab In the label tab you can enter the text for the labels. For further information, refer to chapter 6.16.
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6. Software "Comments" tab Frequently used comments can be entered into this tab. Button NEW Clicking on this indication opens the dialog box "Comment on section" Here you can enter a comment on a section. Clicking on OK saves the entry and closes the window. Clicking on Cancel closes the "Comment on section" dialog box. Any entry made is not saved. CHANGE button Clicking on this indication opens the dialog box "Comment on section". The existing entry can be changed.
6. Software 6.5.4 Reference voltages Indication of the current reference voltage in Volt. Buttons 1 to 8 Clicking on these buttons changes the reference voltage. The individual reference voltage values are predefined and cannot be changed. The display will read the new selected value. Activated buttons will light up in green. 6.5.5 Language selection The flag buttons indicate the languages that can be selected.
6. 6.6 Software Temperature curves Click on the temperature curve symbol to open the corresponding window. Work space Diagram The window contains the following window panes: • Work space • Diagram The "Temperature curves" window is only for failure analysis by Leica Service engineers.
6. Software 6.6.1 Work space REFRESH button Clicking on this button updates the diagram display. RULER button If you click on this button, a ruler will be displayed in the diagram. Use the mouse cursor to move ruler to a certain measuring value position in order to read the measured value more exactly. PRINT button Clicking on this button creates a screen printout of the current diagram display. REZOOM button Clicking on this button resets the diagram display back to how it was.
6. Software Clicking on one of the check boxes in the work space, the 8 temperature sensors of the instrument can be displayed individually in the diagram. The font color of the check boxes helps to identify the corresponding measured values in the diagram, which are displayed in the same color. The following check boxes can be activated / deactivated via a mouse click: • • • • • • • • T1 to, T2 to 2, T3 tv1, T4 tv Head II, T5 tv2,I, T6 tv2,II, T7 tHg2, T8 Box.
6. Software 6.6.2 Diagram The diagram indicates the following: • Temperature in degree centigrade on the y-axis • Time and date on the x-axis • Graphical representation of the 8 temperature measuring points in the instrument by colored lines The diagram can be enlarged as follows: 1. Within the diagram, click and hold the left mouse button. 2. With the mouse, create a zoom box on the area to be enlarged by dragging the mouse cursor to the right and downwards at the same time. 3.
6. 6.7 Software Chronological event list Click on "Event list" to open the corresponding window. Work space Event table The events list is a table displaying the events chronologically, in order of date and time. In this list, all events concerning the program are saved.
6. Software 6.7.1 Work space FILTER button Clicking on this indication opens the dialog box "Filter" Here you can define a time filter limiting the event table indication to one day, one week or one month. To set a filter, proceed as follows: 1. Click on one of the check boxes DAY, WEEK or MONTH to define the desired time interval. 2. In the calendar field, select the desired start day for the event information.
6. Software Comment Clicking on an event in the events list activates the "Comment" field. You can enter a comment regarding the selected event. After confirmation with the ENTER key, the note will be displayed in the event table. Number Number of events displayed in the events list. PRINT button Clicking on this button creates a screen printout of the current display. EXPORT FILE button Click on this button to export the events table into a text file.
6. Software 6.7.2 Event table The table is divided into the following columns: • Date/time Date and time of the events. • User Name of the user who was logged in when the event occurred. • Message Event messages (short description of event). • Comment Comments on the events. For further information, refer to chapter 6.7.1. Individual events messages will be displayed on the events list for a maximum of 90 days from the date when the event occurred. After that, the data will be saved into a text file.
6. 6.8 Software Alarm list Click on the "Alarm list" symbol to open the corresponding window. Work space Event table Chronological display of the alarm list as a table, in order of date and time. In this list, all events concerning the program are saved.
6. Software 6.8.1 Work space In the work space the following parameters can be selected: FILTER button Clicking on this indication opens the dialog box "Filter" For further information, refer to chapter 6.7.1. Comment Clicking on an event in the alarm list activates the "Comment" field. You can enter a comment regarding the selected event. After confirmation with the ENTER button, the note will be displayed in the event table. Number Number of alarm messages displayed in the alarm list.
6. Software 6.8.2 Event table The table is divided into the following columns: • Occurred Indication of date and time when the alarm started • Cleared Indication of date and time when the alarm stopped • Confirmed Indication of date and time when the registered user confirmed the alarm message. For further information, refer to chapter 6.8.1. • User Name of the user who confirmed the alarm. • Message Indication of alarm messages.
6. Software 6.8.3 Error codes: meaning and troubleshooting Error code Error message Trouble shooting Error 01 Knife holder exceeded. Error 02 Limit position of sledge exceeded • Re-initialize instrument. If the problem recurs, call Technical Service. Error 03 Chamber – Alarm temperature exceeded • Check whether there has been a power failure! • Check the selected alarm temperature value. It must differ at least 5 K from the chamber temperature selected.
6. 6.9 Software Chamber illumination Click on the symbol to switch on the illumination of the cryochamber. Another mouse click will switch the chamber illumination back off. Active icons will light up in yellow. 6.10 Section documentation Click on the "Section documentation" symbol to open the corresponding window.
6. Software 6.10.1 Tree diagram Indication of all main studies or substudies in a tree diagram. For navigation purposes, click on the preceding plus or minus sign. The different study types are identified by the preceding colored folder symbols: • Yellow folder symbol Main studies (several). • Green folder symbol Main study. • Blue folder symbol Substudy. • Yellow folder symbol "saved" Main studies (several).
6. Software 6.10.2 Studies protocol By clicking on a main study in the tree diagram, the following indication will be displayed in the "Studies protocol" area: The table is divided into the following columns: • No. Running number automatically allocated by the application. • Date Date when the main study was created. • Name of main study Name of main study displayed. To create a new main study or substudy proceed as follows: 1. Right-mouse-click on a spot anywhere in the upper table.
6. Software When clicking on an existing substudy in the tree diagram the following indication will be displayed in the "studies protocol" area: The table is divided up as follows: • No. Indication of the running number automatically allocated by the application, in the order of date of creation of the main study.
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6. Software 6.10.3 Event table If you click on a main study or substudy in the tree diagram, the following indication will be displayed in the "Event table": The table is divided into the following columns: • No. Indication of the running event number automatically allocated by the application. • Date/time Indication of date or time when the event occurred. • User Indication of the user who was logged in when the event occurred. • Event Indication of the event message.
6. Software 6.11 Photo mode Clicking on the photo symbol activates or deactivates the "Photo mode" and moves the microtome sledge to the mechanical limit stop. When active, the symbol will light up in green. The photo mode enables you to position the specimen or rather the microtome sledge exactly for photos to be taken after each section. In "Parameter settings" the "Photo position dwell time" and the "Photo position triggering time" can be selected.
6. Software 6.13 Remote inquiry Clicking on the "Remote inquiry" symbol opens the dialog box "Safety check - initialization". Carry out the required safety query and subsequently confirm each safety query by mouse click on the corresponding check box. Having confirmed all safety queries, click on OK. The pcAnywhere application will be started. The pcAnywhere window opens up. Note: The latest pcAnywhere version will always be displayed.
6. Software To establish a connection proceed as follows: 1. Doubleclicking on Icon "CM3600 XP" establishes the connection. The "pcAnywhere window waiting" window will open up. Click on CANCEL to stop the connection from being established. After the connection has been successfully established, the window "Remote Connection Attempted" opens up. 3. Click on YES to enable access to the PC of the Leica CM3600 XP. Click on NO to deny access.
6. Software 6.14 Help Clicking on the "Help" symbol starts Adobe Acrobat Reader® and opens the manual in pdf format. 6.15 Saving Click on the "Saving" symbol to save the section data. "Saving" must be clicked on as a first step to enable section documentation via the foot switch. For subsequent steps, refer to chapter 6.17. 6.16 Printing labels Clicking on the label printing symbol opens the "Label printing" window. When activated, the icon will light up in green.
6. Software 6.18 User log-in / log-out Clicking on the symbol logs off the user currently logged in. Click again to open the "Start-and-login" window. For further information, refer to chapter 6.2. Logging off does not automatically stop or delete the actions and settings initiated in the application. 6.19 Quitting the application Clicking on the "Quit" symbol closes the application. When quitting the application, all settings made are reset. Any application steps still in progress are terminated.
7. Operating the Leica CM3600 XP To ensure proper operation of the Leica CM3600 XP as well as use of the specimen stages and the specimen blocks themselves, we strongly recommend to attend a Leica application training. 7.1 Check list The checklist below provides an overview on how to successfully operate the Leica CM3600 XP. We recommend that you make a copy of this checklist and attach it to an instrument surface where it is clearly visible to ensure that all points of the list are observed. 1.
7. 7.2 Operating the Leica CM3600 XP Switching the instrument on Proceed as follows: 1. Switch on the mains switch. For further information, refer to chapter 5.3. The refrigeration and control unit is activated. 2. Switch on the PC and peripherals. For further information, please refer to the manual of the PC. 7.3 Starting the software Proceed as follows: 1. Carry out the software "Start and log-in" procedure. For further information, refer to chapter 6.1.
7. Operating the Leica CM3600 XP 7.5 Specimen stages This chapter provides instructions on how to insert the knives. There are three different types of specimen stages: • Special holder, orientable, with ball-and-socket joint, • Specimen stage, orientable, with ball-and-socket joint, • Specimen stage, non-orientable.
7. Operating the Leica CM3600 XP When inserting the specimen stages, bear in mind the following: • Prior to sectioning, the specimen stage should have been stored inside the cryochamber for at least 24 hours, to make sure it has the correct sectioning temperature (approx. -20 °C). • To mount the entire block (specimen block on specimen stage) onto the sledge, first bring the knife holder to its upper limit position. For further information, refer to chapter 6.4.5.
7. Operating the Leica CM3600 XP 7.5.1 Inserting the specimen stage, orientable, with ball-joint To insert the specimen stage, orientable, with ball-and-socket joint proceed as described below. The special holder, orientable, with ball joint is inserted the same way. Tools required: • Fork wrench (size 17). Proceed as follows: 1. Use the knee lever to move the sledge to the right-side reversal point. For further information, refer to chapters 3.2.2 and 6.4.9. 2.
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7. Operating the Leica CM3600 XP 7.5.2 Inserting the specimen stage, non-orientable Tools required: • Allen key (size 6). Proceed as follows: 1. Use the knee lever to move the sledge to the right-side reversal point. For further information, refer to chapters 3.4.2 and 6.4.9. 2. Comply with the safety instructions provided in chapter 7.5. 3. Open the window. For further information, refer to chapter 3.2.3. 4. Remove screw (1). 5 Lift off T-piece (2) and support ring (3). 6.
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7. Operating the Leica CM3600 XP 7.6 Knife This chapter provides instructions on how to insert the knives. Two types of knives can be used in the CM3600 XP: • Standard knives • Disposable blade holder with disposable blade Since there is a considerable risk of injury, please be sure to comply with the following safety instructions: Prior to any work involving the knife, microtome or inside the cryochamber, activate the emergency stop switch. For further information, refer to chapter 3.4.1.
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7. Operating the Leica CM3600 XP 7.6.1 Inserting standard knives To insert a standard knife, proceed as follows. Tools required: • Allen key (size 4). Proceed as follows: 1. Use the knee lever to move the sledge to the right-side reversal point. For further information, refer to chapters 3.2.2 and 6.4.9. 2. Comply with the safety instructions provided in chapter 7.6. 3. Open the window. For further information, refer to chapter 3.4.3. 4. Remove screws (1) and remove pressure plate (2). 5.
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7. Operating the Leica CM3600 XP 7.6.2 Inserting the disposable blade holder with disposable blade To insert the disposable blade holder with disposable blade proceed as described below. Tools required: • Allen key (size 4). • Allen key (size 2.5). Proceed as follows: 1. Use the knee lever to move the sledge to the left-side reversal point. For further information, refer to chapters 3.4.2 and 6.4.9. 2. Comply with the safety instructions provided in chapter 7.6. 3. Open the window.
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7. Operating the Leica CM3600 XP 7.6.3 Adjusting the knife angle Tools required: • Allen key (size 5). Proceed as follows: 1. Comply with the safety instructions provided in chapter 7.6. 2. Loosen screws (1). 3. Rotate knife holder (2) selecting the desired knife angle on scale (3) (recommended angle = 20°). 4. Tighten screws (1).
7. 7.7 Operating the Leica CM3600 XP Setting the trimming parameters Proceed as follows: 1. On the computer screen, open the "Main window". Select the desired section thickness, sledge speed, cutting window and sectioning program. For further information, refer to chapter 6.4. For reasons of safety and accuracy, the maximum trimming thickness should not exceed 100 μm per section. When trimming biological material (particularly bone), section thickness should not exceed 50 μm. 2.
7. Operating the Leica CM3600 XP 7.8 Setting the sectioning parameters Proceed as follows: 1. On the computer screen, open the "Main window", select "MANUAL" mode of operation. Subsequently, select the desired section thickness and the cutting window. For further information, refer to chapter 6.4. 7.8.1 Sectioning the specimen For the sectioning procedure, you need brush, sectioning tool and special section collecting tape and - depending on the application - a dehydration frame.
7. Operating the Leica CM3600 XP Proceed as follows: 1. Select manual mode of operation and the desired section thickness. 2. Activate the section documentation function. 3. Use the knee lever to move the sledge to the right-side reversal point (left edge of the specimen block just about clears the knife). 4. Cut the section and clean the specimen surface with the brush. 5. Cut off a desired length from the tape roll.
7. Operating the Leica CM3600 XP 15. Add the section data to the documentation. 16. Attach the section to a dehydration frame which has already been kept in the cryochamber for a while. 17. To speed up the dehydration process once you are done with the sectioning work, the automatic or manual dehydration function of the instrument can be activated. 7.9 Dehydrating the sections To be able to store the sections or use them otherwise outside the cryochamber, they must be properly dehydrated.
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8. Cleaning and Maintenance 8.1 Cleaning Switch the instrument off before each cleaning. Wear safety gloves when cleaning the instrument. When using cleaning agents, observe the manufacturer's safety instructions and the laboratory regulations valid in the country of use.
8. Cleaning and maintenance Tools required: • Allen key (size 6). Proceed as follows: 1. Comply with the safety instructions provided in chapter 3. 2. Switch the instrument off and unplug from mains, see chapter 5.3.1. 3. Extract the knife from the knife holder, see chapter 7.6. 4. Lock the window, see chapter 3.4.3. 5. Open the cabinet lid, see chapter 3.4.4. 6. Remove screws (1) and screws (2).
8. Cleaning and Maintenance 8.2 Maintenance The instrument requires maintenance from Leica Service at an interval of once per year. The pushbutton switch of the refrigeration must likewise be pressed at least once a year. 8.2.1 Oiling Be sure to check the oil level every and before each sectioning procedure. Checking the oil level Unscrew both lids (1). The oil level must always be above the small capillary tubes (2).
8. Cleaning and maintenance 8.2.2 Defrosting Three different defrost procedures can be selected: • Automatic defrost I • Automatic defrost II • Manual defrost Automatic defrost I This defrost cycle takes place automatically once every 24 hours. The defrost cycle itself is mandatory, i.e. cannot be deactivated by the user, while the start time of the cycle is freely selectable. For further information, refer to chapter 6.5.1.
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9. Optional accessories Section collecting tape Section collecting tape, 38 mm x 66 m, type 810 Section collecting tape, 76 mm x 66 m, type 810 Section collecting tape, 148 mm x 66 m, type 810 Section collecting tape, 110 mm x 66 m, Tesa 4248 Section collecting tape, type 810, for autoradiography.
