Leica CE/ Leica CN Knife holder Instruction Manual Leica knife holder CE/ knife holder CN V2.2 English – 08/2005 Always keep this manual near the instrument. Read carefully prior to operating the knife holders.
NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual. For erroneous statements, drawings, technical illustrations etc.
1. Operation Danger! Microtome knives and blades have extremely sharp cutting edges! For disinfection we recommend Leica Cryofect disinfection spray. Mounting the knife holder base 1. Mount the knife holder base (1) as shown in Fig. 1. 2. Rotate lever (2) clockwise to clamp. 1 2 Fig. 1 When inserting the knife holder base, apply sufficient pressure towards the left to overcome the resistance of the spring in the bottom part of the knife holder. 3 4 5 Mounting the knife holder 1.
1. Operation Mounting the anti-roll system 1. Insert the glass plate into the changing frame and clamp evenly with the knurled screws (7). 2. From above, insert the axle (8) of the metal frame into the opening of the swivel arm of the anti-roll system (4, Fig. 2), so that the pin (6) rests in the groove (5, Fig. 2). 3. From below, slide the white plastic washer (9) onto axle (2). 4. Screw knurled nut (10) onto axle (2) from below. 7 7 6 8 9 10 Fig. 3 13 12 11 Fig.
1. Operation Inserting the knife into knife holder CN and knife height adjustment 15 15 Fig. 6 15 16 16 Fig. 7 Knife holder CN can be operated with all wedgeshaped c- and d-profile knives. To fit disposable blades into knife holder CN, use the blade holder for disposable blades. Prior to inserting the knives (from above) completely loosen the two clamping screws (15). Adjust the knife height before retightening screw (15). The knife height is adjusted via the knurled nuts (16).
1. Operation Finger guards 19 The finger guards are integrated into the clamping jaws. Use handles (19) to slide them back and forth. The width of the finger guards is sufficient for knives up to 22 cm long. Once you finish sectioning, always cover the knife edge with the finger guards. Fig.
1. Operation Knife support bar Ensure that the recess (24) is pointing away from the microtome. Danger! Microtome knives and blades have extremely sharp cutting edges! Never try to catch a falling knife! 24 Fig. 12 Clearance angle adjustment The harder the specimen the wider the clearance angle. However: the wider the clearance angle the greater the compression of the sections. Clearance angle adjustment - knife holder CE 25 Fig.
1. Clearance angle adjustment - knife holder CN 27 26 Fig. 14 Operation Prior to setting the clearance angle, the knife edge must be located exactly in the spin axis of the knife holder. First, set a clearance angle of ‘0’. To do so, loosen lever (23, see Fig. 11) and bring the index marker (26) in line with the figure ‘0’ on the clearance angle scale (27). Retighten the lever (23).
1. Operation Use the knurled nut to height-adjust the anti-roll system: • rotate counterclockwise to move the anti-roll system towards the knife; • rotate clockwise to move the anti-roll system away from the knife. If the anti-roll system is not correctly adjusted relative to the knife edge, the following problems occur: I I II III 10 Fig. I: The sections roll up on the glass plate of the anti-roll system. Problem: glass plate situated too low.
1. Operation Cleaning the knife holder CE Daily cleaning procedure 1. Tilt the anti-roll system (33) to the left, hold at lever (34) while doing so. 2. Loosen clamping lever (35) of pressure plate. 3. Now you can remove the pressure plate (36) for cleaning (use alcohol or acetone). 33 34 36 35 For disinfection purposes, standard commercial detergents and disinfectants can be used – our recommendation is Leica Cryofect. Fig.
2. Ordering information for knife holders CE / CN Ordering information - knife holder CE • • • • • • • • • • Knife holder CE for low-profile blades, assy. Knife holder CE for high-profile blades, assy.
3. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
Notes 14 Instruction manual V 2.
