Instructions for Use Leica EG1150 H Paraffin Embedding Station Leica EG1150 H V 2.5, English 09/2014 Order No.: 14 0388 81101 RevF Always keep this manual with the instrument. Read carefully before working with the instrument.
IMPORTANT NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Table of contents 1. Important Information................................................................................................................................. 5 1.1 1.2 1.3 1.4 Symbols in the text and their meanings.................................................................................................... 5 Intended use of instrument.......................................................................................................................... 6 Qualification of personnel.
1. Important Information 1.1 Symbols in the text and their meanings Warnings appear in a gray box and are marked by a warning triangle . Notes, i.e. important user information appear in a gray box and are marked by an . information symbol Solvents and reagents that are inflammable are marked with this symbol. This warning symbol indicates the surfaces on the instrument that are hot during operation. Avoid direct contact to prevent risk of burning.
1. Important Information 1.2 Intended use of instrument The Leica EG1150 H is a modern paraffin embedding station with microprocessor control system. It is designed for embedding histological tissue specimens in molten paraffin for use in pathology laboratories and only for the following tasks: • Melt solid paraffin for sample embedding and maintain the molten paraffin at the required temperature. • Pour paraffin into embedding molds where the specimens are placed.
2. Safety The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. 2.1 Safety notes These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument.
2. Safety 2.2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Operating the instrument safely is, above all, the responsibility of the owner, as well as the designated personnel who operate, service or repair the instrument. To ensure trouble-free operation of the instrument, make sure to comply with the following instructions and warnings.
2. Safety Safety instructions - working with the instrument Paraffin is flammable and should therefore be handled with due care. Do not use sharp tools to remove solidified paraffin from the work areas, as this may destroy the coating on the surface. Use the plastic spatula supplied with the instrument. During operation, the paraffin reservoir, embedding mold tray, cassette tray, work area as well as the forceps holder are hot.
3. Instrument Components and Specifications 3.1 Technical data General data Approvals: The instrument-specific approval marks are located on the rear panel of the instrument next to the type plate. Voltage of power supply: 100-120 V, 50/60 Hz 230-240 V, 50/60 Hz Power consumption: 1100 VA Protection class1: I 1 Pollution degree : 2 Overvoltage category: II Operating temperature range: +18 °C to + 40 °C Operating temperatures: 55 °C to 70 °C, adjustable in 5 K increments. Relative humidity: Max.
3. Instrument Components and Specifications 3.2 Overview — instrument components Standby switch Paraffin reservoir with lid Control panel Work area illumination Dispenser Lid Cassette tray Cassette tray Forceps holder Work area Lid Embedding mold tray Embedding mold tray Cooling spot Paraffin collection tray left Paraffin collection tray right Fig.
3. Instrument Components and Specifications Instrument rear view Secondary fuses F1-F3 Secondary fuses F4-F5 Heat sink Port for power cord Port for foot switch Sliding elements Fig. 3 3.3 Instrument specifications • Paraffin reservoir with a capacity of 3 liters. • The paraffin flow is activated by means of a height-adjustable, pivotable clip - activated either manually by the mold or a foot switch (optional). • Controllable flow rate.
4. Instrument Setup 4.1 Unpacking and installation When the instrument is delivered, check the tilt indicators on the packaging. If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage. These unpacking instructions only apply if the box is placed with the symbols facing upwards for unpacking. 1 2 1. Remove 8x screws (1) for the cover (2). 2.
4. Instrument Setup 8 7 6 5 5 6 Fig. 5 4. In order to lift the instrument (5) take hold of it only at the lower housing base in the front and rear (arrow in the detail for Fig. 5) and lift it off of the bottom pad and out (6). Important! Lifting the instrument by the dispenser (7) or paraffin reservoir (8) can cause serious damage. The packaging must be retained for the duration of the warranty period. To return the instrument, follow the instructions above in reverse order. 4.
4. Instrument Setup 4.
4. Instrument Setup 4.4 Necessary assembly work Install the following accessories and make the appropriate adjustments to make the instrument ready for use: • • • • • Install accessories. Adjust instrument feet if necessary. Install magnifier (optional). Connect foot switch (optional). Connect to the power supply. Install accessories Install embedding mold tray (2) and cassette tray (3).
4. Instrument Setup Installing the accessories 9 8 7 4 5 2 3 11 11 10 1 6 6 Fig.
4. Instrument Setup 4.5 Optional accessories Magnifying glass The magnifying glass provides a magnified view of the work area. When properly adjusted, an enlarged view of the dispenser and cooling spot is available. 5 1 1 2 3 4 5 4 3 Fig. 7 Installing the magnifying glass • On the dispenser (5) a tap hole (1) is provided, which is closed with a nylon screw (2). • Remove the screw (2) with a screwdriver and store it in a safe place.
4. Instrument Setup 4.6 Electrical connection The instrument MUST be connected to a grounded power socket. The instrument is supplied with a set of different power cords. Only the power cord intended for the local power supply (socket) may be used. Do not use an extension cord. 1 4 6 2 3 5 Fig. 9 Before connecting the power cord to the foot switch, ensure that the standby switch (1) (control panel, front) is set to "O" ("O" = OFF).
5. Operation 5.1 Instrument parts/functions 1 2 11 Recycled paraffin may NOT be used in the Leica EG1150 H due to the danger of contamination. 1 14 13 13 2 12 Fig. 10 The flow cannot be stopped completely with the metering screw (11). It must not be turned while cold! 20 Paraffin reservoir (1) The paraffin reservoir has a capacity of approx. 3 liters. The paraffin temperature can be set between 55 °C and 70 °C in 5 K increments.
5. Operation Work top (3) The temperature of the work top can be adjusted between 55 °C and 70 °C in 5 K increments. This includes the embedding area, forceps holder (5) and the cooling spot (4). The working area has a groove around it and several drain holes (15), through which excess paraffin rapidly drains. 5 3 4 3 Cooling spot (4) The cooling spot is an integral part of the work area.
5. Operation Paraffin collection tray (8) Two indirectly-heated paraffin collection trays for excess paraffin are located under the work surface. 7 6 The paraffin collection tray must be emptied daily. To prevent contamination, do not reuse paraffin collected in this tray. If the instrument is operated without the paraffin collection trays there is risk of burning. 8 8 17 16 Fig. 12 When working with half-open lids (17), raise the temperature one level to ensure that the paraffin remains molten.
5. Operation Standby switch (8) After the unit has been commissioned, the standby switch should only be used if the unit is to be switched off for a longer period of time. 21 8 20 In daily routine operation, the ON/OFF button on the control panel should be used. Pressing the ON/OFF button (20) switches the instrument into active standby mode. All displays are switched off, only the LED (21) of the ON/OFF button remains lit.
5. Operation The foot switch (22) actuates the magnet valve of the dispenser. It must be connected as described in Chapter 4.6. Foot switch (22) optional 22 11 14 12 The plug of the foot switch MUST be screwed to the socket. Otherwise hot paraffin can escape, even if the switch is not activated. Pressing the foot switch opens the valve, releasing it closes it. This keeps the operator's hands free to work with the instrument. The flow volume can be adjusted with the metering screw (11).
5. Operation 5.2 Switching the instrument on • Activate the standby switch (left-hand side, next to control panel) (see Fig. 15, 17). • All LEDs of the operating panel light up briefly and the installed software version is shown on the display for around 2 seconds. All displays go out, the unit changes to standby mode. The green LED in the ON/OFF button indicates that the instrument is ready to operate. Fig. 15 • Press the ON/OFF button down for approx. 2 seconds to switch over to the operating mode.
5. Operation 5.3 Control panel functions The control panel beside the standby switch (8) consists of a foil keyboard with push buttons, LEDs and a single-line display. It is divided into two control areas: temperature setting and display and instrument control and programming. 8 Temperature control and display LED display for the individual heating areas Set time 6 5 1 2 3 26 Display: standard indication is real time Fig.
5. Operation 5.4 Operating modes 21 Standby mode The display and all LEDs are switched off in standby mode. The unit switches on or off automatically at the programmed start and ending times. Only the green LED (21) in the ON/OFF button indicates that the instrument is ready to operate. Enabling operating mode • Hold down the ON/OFF button for approx. 2 seconds. • The instrument switches from standby mode to operating mode.
5. Operation 5.5 Time-program control The value shown in the display must be set to the current local time to ensure the correct operation of the time-program control. Setting the time • Press the CLOCK button as many times as required until the green "TIME" LED lights up. • Set the current time with the / buttons. The required value is reached faster when holding down the button in question.
5. Operation Weekdays/working days The automatic switch-on feature is linked with the individual weekdays. It is therefore necessary to define the days for which the automatic switch-on feature shall operate. Only on those weekdays that have been defined as working days, the instrument will be at the required temperature and ready to operate. Setting the current weekday: • Press the DAY button as many times as required until the green "TODAY" LED lights up.
5. Operation Setting temperature values 5.6 Instrument heater The temperatures for the instrument's four heating areas can be adjusted separately from 55 °C to 70 °C in 5 K increments. 1 2 3 When setting the temperature, please observe the paraffin manufacturer's specifications for the maximum permissible temperature. 4 Fig.
5. Operation Advance times Display To ensure that the instrument is ready (all working temperatures reached) at the programmed start time, the various sections are activated in advance as follows: Paraffin tank: Heating starts 4 hours before working time begins. Work top: Heating starts 4 hours before working time begins. Dispenser tube: Heating starts 1 hour before working time begins. Cooling spot: Cooling starts 1 hour before working time begins.
6. Maintenance and Cleaning 6.1 Cleaning the instrument Do not use xylene for cleaning. Xylene vapors are heavier than air and can ignite at a considerable distance from the source of heat. A fire hazard exists! To avoid scratching the surface of the instrument only the plastic spatula that is supplied with it should be used for cleaning – on no account use metal tools! Work surfaces • All common laboratory cleaning products suitable for the removal of paraffin (e.g.
6. Maintenance and Cleaning Paraffin collection tray • Before the paraffin collection tray drawers can be emptied, any excess paraffin on the work area must be removed with cellulose wadding in order to prevent any paraffin from penetrating into the instrument. Use care with paraffins with a low melting point - risk of burning when removing the paraffin collection trays due to liquid paraffin. • Only remove and empty the paraffin collection trays while they are warm.
7. Troubleshooting 7.1 Possible faults This chapter will help you remedy problems that may occur when working with Leica EG1150 H. If a problem cannot be remedied by following the instructions in this chapter, please contact your Leica technical service center. For further instructions, please refer to Chapter 8. The table below lists the most common problems that may occur as well as possible causes and remedies. Problem 1. Display: The in the display.
7. Troubleshooting Problem Possible cause 3. Paraffin reservoir Paraffin does not melt or melts only - Selected temperature too low. very slowly. - No enhanced mode set. Corrective action - Increase temperature for paraffin reservoir. - Activate on-demand heater. (see Chapter 5.6) - Temperature indication incor- - Check temperature settings and repeat if necessary. rect, or paraffin reservoir is not heated. - Incorrect start time selected. - Check start time.
7. Troubleshooting Problem 5. Illumination does not work. Possible cause Corrective action - Button on the head of the lamp - Press the On/Off button on the has not been pressed. head of the lamp. - Bulb defective. - Ask technical service to replace the lamp. - Fuse defective. - Change fuse. (See Chapter 7.3) 6. Individual heating areas (Despite correct temperature indi- - Fuse for this heated area has - Change fuse. cation) heating does not work. responded. (see Chapter 7.3) - Heater defective.
7. Troubleshooting 7.2 Changing a fuse Before changing a fuse switch the instrument off and pull out the power plug. Use ONLY the provided replacement fuses. Important! The instrument has a number of circuits that are protected by fuses with different values. Observe the following instructions exactly to ensure that the correct spare fuse is used for the matching fuse holder. The instrument has five electrical areas with separate secondary fuse protection.
7. Troubleshooting Select the correct spare fuse This marking (2A) on the fuse and the labeling next to the fuse holder on the rear of the instrument MUST agree! On the back there are five different fuse holders (Fig. 24). One spare fuse must be selected from the five spare fuses available that has the marking 2A written on it. (see Fig. 25, left) Only the spare fuse with the marking 2A may be inserted in this fuse holder (T2A). This is also applicable in the same way for the other fuse holders. Fig.
8. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
Decontamination Certificate (Master) Decontamination Certificate 9. Dear Customer, Any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems for repair.
** Such equipment must not be returned without the written agreement of Leica Biosystems. Yes No equipment has been prepared to ensure safe handling/transportation. 9. Decontamination Certificate (Master) 3 The Whenever possible, please use the original transportation case/box. Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging, or hand it over to the service engineer.
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