/HLFD /3 & Laser Printer for Cassettes Operating Manual Leica LP C V1.0 English - 06/2010 Always keep this manual with the instrument. Read carefully before working with the instrument.
NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Table of Contents 1. 2. Important Information ................................................................................................................ 5 Safety ............................................................................................................................................ 6 2.1 2.2 3. Instrument Components and Specifications ....................................................................... 10 3.1 3.2 4. Cleaning the laser lens ..................................
1. Important Information Symbols in the text and their meanings Dangers, warnings and cautions appear in a gray box and are marked by a warning triangle . Notes, i.e. important user information appear in a gray box and are marked by an information symbol . (5) Numbers in parentheses refer to item numbers in illustrations. LOAD Function keys to be pressed on the touch screen are written in bold-print capital letters.
2. Safety Be sure to comply with the safety instructions and warnings provided in this chapter. Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. 2.1 Safety notes This Operating Manual contains important instructions and information regarding the operational safety and maintenance of the instrument.
2. Safety Warnings – Transport and installation • Only connect the instrument to a grounded power socket. The protective effect may not be eliminated by an extension cable without a protective grounding conductor. • Do not operate in rooms with explosion hazard. • Exposure to extreme temperature changes between storage and installation locations and high air humidity may cause condensation inside the instrument. In this case, a two-hour waiting period must be maintained before switching on the instrument.
2. Safety Warnings – Cleaning and maintenance • Before any maintenance, switch off the instrument and unplug it from power supply. • Do not use alcohol, detergents containing alcohol (window cleaner!), abrasive cleaning powders and/or solvents containing acetone or xylene for cleaning. Xylene or acetone will damage the finished surfaces! • Prevent liquids from entering the interior of the instrument while the instrument is being cleaned or during operation.
2. Safety • Never use the Leica LP C Laser Cassette Printer without ensuring that the filter unit is connected properly and is working. Doing so will cause the instrument to malfunction and expel harmful dust particles. • Always set up the Leica LP C on a stable, flat surface. Set up the LP C Laser Cassette Printer so that the front and rear operating elements are easily accessible to the operator.
3. Instrument Components and Specifications 3.1 Overview – instrument components 2 3 1 7 4 8 5 9 1 2 3 4 10 - Basic instrument Monitor (optional) Cassette magazine Housing for the mirror device of the laser 5 6 7 8 9 - 6 Scanner (optional) Output chute for cassettes Online button Power LED Emergency stop button Fig. 2 Operating Manual V 1.
3. Instrument Components and Specifications Rear panel and electrical connections 10 11 12 13 14 15 17 16 19 21 18 20 Fig. 2 10 11 12 13 14 15 16 - Identification label Warning sticker: CLASS 1, laser product Serial connection No.
3. Instrument Components and Specifications 3.2 Technical data General Approvals: Nominal voltage: Nominal frequency: Classification: Laser classification: Secondary fuses: Operating temperature range: Relative humidity: Barcode compatibility: Connectivity to computer: The instrument-specific approval marks are located on the rear side of the instrument next to the identification label.
3. Instrument Components and Specifications Dimensions of filter unit: Width x depth x height: Electrical requirements: 210 mm x 490 mm x 460 mm The filter unit is powered directly by the cassette printer. Filter compatibility: Use filters developed specially for this filter unit only. Contact a Leica employee for ordering instructions.
4. Instrument Setup 4.1 Unpacking instructions When the instrument arrives, check the tilt indicator (1) on the package. If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage. If it is evident that the shipment was damaged during transport, please make a claim to the carrier immediately.
4. Instrument Setup 4.2 Standard delivery The Leica LPC standard delivery includes: Box 1 Filter Unit-120 V or Filter Unit-240 V End pieces for filter outlet Box 2 LP C - Laser Cassette Printer 120 V ............................................................................................ 14 0605 46656 or LP C - Laser Cassette Printer 240 V ............................................................................................
4. Instrument Setup 4.3 Assembling the filter unit Never operate the Leica LPC printer without the filter unit. 1a 2 Fig. 7 3, not visible • You need the filter unit (1a) and accessory (2) cartons from the transport crate. • After unpacking, remove the four knurled screws (3) and lift the lid (4) off of the lower part. • Reach into the opening of the prefilter housing as shown in Fig. 9 and pull the bottom upwards briefly, then forwards and out in order to remove the inserted accessories (6).
4. Instrument Setup 37 30 • Hook the prefilter (37) - depending on your requirements, either at the left or right - into the filter unit (30). • Insert the clips (31, Fig. 11) back through the cover and into the housing of the filter unit and fasten the cover in place by inserting and tightening the four knurled screws (see cutout, Fig. 11) - to do so, push each clip (31) back and downwards slightly so that the knurled screw can engage the thread. Fig. 10 31 31 Fig.
4. Instrument Setup 32 34 36 37 38 32 32 35 Fig. 12 • Place connecting pieces (32) for the hoses on the spouts at the correct angle, and seal off the inlet you do not need using the plug (34) provided. The connecting pieces (32, 34) have to be installed BEFORE the cover is attached to the main filter and before the prefilter is attached to the side of the filter housing. Failure to observe this instruction can result in breakage of the cover and the prefilter housing.
4. Instrument Setup 4.4 Cassettes Only Leica LPC cassettes may be used for the Leica LPC Laser Cassette Printer: A - LP C tissue cassette B - LP C fine-mesh tissue cassette C - LP C biopsy cassette A B C Fig. 13 The cassettes are offered in various colors - for more information, refer to Chap. 6, Optional Accessories. • Remember that minor color changes can occur in LP C cassettes if these are exposed to reagents that contain Picric acid, e. g. Bouin's fixative.
4. Instrument Setup 4.5 Installation site requirements The installation site must be well-ventilated. Never operate the instrument in rooms with explosion hazard. • The printer needs an installation area of approx. 260 x 460 mm and the filter unit needs an additional installation area of approx. 470 x 490 mm. Additional space is required for the PC containing the control software. • Stable laboratory bench and practically vibration-free floor. • Room temperature consistently between +5 °C and +40 °C.
4. Instrument Setup 4.6 Operating the printer The Cassette Printer contains a housing for the mirror device of the laser (4) and 1 cassette magazine (3) that can hold 70 tissue cassettes (3a). Located directly below the cassette magazine, the gripper mechanism (22) pushes the cassettes out of the magazine, transports them to the marking position and then conveys them downwards through the output opening of the cassette chute (23).
4. Instrument Setup • Safety Interlock (24/25) in the main cover. If the Safety Interlock operates, the user must: • Immediately investigate the reason for the interlock. • Check the housing cover for damage before starting up the Cassette Printer. • If damage is found or the instrument does not work, stop using the Cassette Printer and contact the Leica service technician or Sales Consultant to have the problem remedied. 2.
4. Instrument Setup 4.7 Loading the cassette magazines The correct cassette color and type must be selected by the user. The magazines must be manually loaded with cassettes of the type and color the user intends to print. The Cassette Printer is not capable of identifying what type and color cassettes are placed in the magazines or what magazine is loaded into the machine.
4. Instrument Setup 4.8 Electrical connection The instrument MUST be connected to a grounded power socket. The instrument is delivered with two different power cables. be sure to use only the one that is appropriate for the local power supply (plug must fit on-site wall outlet).
5. Cleaning and Maintenance To keep your Cassette Printer operating properly it is critical that the following parts are cleaned biweekly. • Laser lens • Area around the laser lens • Filter system inlet openings • Optical sensors • After this routine maintenance is completed, the user must check the Cassette Printer for damage. • If the Cassette Printer is damaged, stop using the Cassette Printer immediately and contact the Leica service technician for information on replacement. 5.
5. Cleaning and Maintenance NEVER attempt to turn the lens unit (27) manually. Turning it manually causes long-term damage to your Laser Cassette Printer. 27 Cleaning the Laser Lens Fig. 22 IMPORTANT! Use only 5%-8% acetic acid with distilled water to clean the laser lens. All other solvents will permanently damage the lens. 28 To clean the laser lens (28) follow the following procedure: • Gauze pads in a size of 5 x 5 cm are best suited for lens cleaning.
5. 5.2 Cleaning and Maintenance Cleaning the filter inlet tubes The filter system of the Cassette Printer MUST be cleaned every 2 weeks to maintain proper function. • The silver intake tube (29) is located inside the main cover. 29a Cleaning the Intake Tube The silver intake tube (29) and the exhaust shaft (29a) collect dust if not cleaned every 2 weeks. • Use a gauze pad in a size of 5 x 5 cm to clean the intake tube and the area around the tube. 29 Fig.
5. Cleaning and Maintenance 5.3 Cleaning the optical sensors The Optical Sensors allow the machine to know if it is loaded with cassettes. If the sensors are covered with dust, the printer ceases to operate. Fig. 26 Fig. 25 The sensors will need biweekly cleaning. Locating and Cleaning the Sensors • The location of the sensors is pointed out by the yellow arrows (Fig. 25 and Fig. 26). • Sensors are located on both sides of the housing.
5. CAUTION: Always use dry gauze to clean the main laser system. If liquids drip into the motor or other electrical parts, long-term damage to your Laser Cassette Printer will result. • Use DRY gauze to clean the area around the main laser system carefully. • Blow a little compressed air into this area to clean the optical sensors. Fig. 27 5.4 Cleaning and Maintenance Be sure not to ice over the sensors with the compressed air.
5. Cleaning and Maintenance 5.4.1 Replacing the prefilter The prefilter bag must be changed after every 10,000 - 15,000 cassettes - depending on the type of laser printing (positive or inverted) - or 1x a year. If the laser printer detects a reduction of the air flow rate, an audible signal is sounded by the filter unit and you see the following message on the monitor. When either condition (warning or beeping) is present the user must change the prefilter bag. Fig.
5. Cleaning and Maintenance The prefilter is replaced by opening the prefilter compartment of the filter unit and replacing the prefilter bag (17). • Open the prefilter compartment by pulling the tube end (18) of the plastic cover (19). 19 18 17 To replace the filter, turn it counterclockwise as shown above and pull it off. Remove the filter bag carefully and dispose of it according to the regulations applicable in the country of use.
5. Cleaning and Maintenance 2. Then tilt the tube end on the cover towards the metal plate to ensure secure closing. 3. Make sure that the prefilter bag does not get crushed between the plastic cover and the metal plate and become damaged. · This causes the filter to lose vacuum and releases harmful dust particles. Do not operate the LP C Laser Cassette Printer without ensuring that the filter unit is properly connected and running.
5. To replace the main filter: • Pull the hose ends off of the intake spouts (32). 32 21 Cleaning and Maintenance 8 • Unscrew the four knurled screws (8, see also Fig. 8, page 16) at the top of the filter housing and remove the top cover (21) of the filter unit. 32 32 21 Fig.
5. Cleaning and Maintenance 22 • Remove the filter cartridge (22) by reaching into the opening with both hands and pulling it upwards and out. Fig. 32 When the main filter is installed the top of the filter should be marked by the user with the recommended replacement date. (Is visible when the cover of the main filter unit is removed.) Fig. 33 Bottom of the main filter Fig. 34 34 • Replace the old main filter with a new one and mark the top of the filter with the date for the next replacement.
5. 5.5 Cleaning and Maintenance Replacing fuses Before replacing the secondary fuses, always switch the instrument off first and unplug it from the power supply. The fuses are located on the rear of the instrument. 40 17 17 17a Fig. 35 Fig. 34 • Using a screwdriver (40), pry out the fuse insert (17) and remove it. • Remove the defective fuse from the fuse holder (17) and replace it with a replacement fuse (17a).
6. Optional Accessories Touch PC, 10" ................................................................................................................................. Touch PC holder ........................................................................................................................... Scanner .......................................................................................................................................... Scanner holder ........................................
6. Optional Accessories Color: peach ............................................................................................................................. Color: pink ................................................................................................................................ Color: white .............................................................................................................................. Color: yellow ......................................................
7. Appendix A – Laser Safety 7.1 Laser hazard classes The intent of laser hazard classification is to provide clear distinction of the lasers’ properties and hazards to users so appropriate protective measures can be taken. Classification is based on the maximum output available for the intended use. Specific labeling requirements indicate that the class of the laser as well as the emission wavelength(s) and any other applicable precautionary instructions must be included on any signage.
7. Appendix A – Laser Safety • Class 2: This classification applies only to continuous wave (CW) and repetitive-pulse lasers and laser systems of the visible part of the electromagnetic spectrum (400 – 700 nm) that exceed Class 1 levels, but do not exceed an average radiant power of 1 mW.
7. Appendix A – Laser Safety 7.2 Inspection measures for laser classes Inspection measure Class 1 Class 2 Activation of warning systems No requirement. Interior laser XE optics laser Controlled area Class 3b Class 4 No require- No requirement. ment. Should have. Shall have. No requirement. No require- No requirement. ment. Shall have. Nominal Hazard Zone analysis required. Shall have. Nominal Hazard Zone analysis required. Labels Shall have. Shall have. Shall have. Shall have.
7. Appendix A – Laser Safety 7.3 Biological effects of laser beams 7.3.1 Eye injuries Because of the high degree of beam collimation, a laser serves as an almost ideal point source of intense light. A laser beam of sufficient power can theoretically produce retinal intensities at magnitudes that are greater than conventional light sources, and even larger than those produced when directly viewing the sun. Eye exposure to a direct beam can cause permanent eye damage including blindness.
7. Appendix A – Laser Safety 7.3.3 Skin injuries Radiation in the UVA (315-400 nm) range can cause hyperpigmentation and erythema (sunburn). Exposure in the UVB (280-315 nm) range is most injurious to skin. In addition to thermal injury caused by ultraviolet energy, there is also possibility of radiation carcinogenesis from UVB.
