Instructions for Use Leica TP1020 Tissue Processor Instructions for Use Leica TP1020 V 2.9 RevC, English – 12/2013 Order No. 14 0422 80101 RevC Always keep this manual with the instrument! Read carefully before working with the instrument.
NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Table of contents 1. 2. 3. 4. 5. 6. 4 Important Information.............................................................................................................................. 7 Safety.......................................................................................................................................................... 9 2.1 2.2 Safety notes.......................................................................................................................................
Table of contents 6.2.6 6.2.7 6.2.8 7. 8. 9. 10. 11. 12. Selecting infiltration times per station..................................................................................................... 47 Displaying total program duration............................................................................................................ 48 Leaving the programming mode...............................................................................................................
Table of contents 13. 14. 15. 16. 17. 6 Optional accessories............................................................................................................................. 82 13.1 13.2 13.3 13.4 Three-level tissue basket........................................................................................................................... 82 Basket removal device............................................................................................................................
1. Important Information Symbols used in the text and their meaning Dangers, warnings and cautions appear in a gray box and are marked by a warning triangle . Notes, i.e. important information for the user, appear in a gray box and are marked with the symbol . (5) Numbers in parentheses refer to item numbers in illustrations. Symbol for labeling electrical and electronic equipment in accordance with Section 7 of the German Electrical and Electronic Equipment Act ( ElektroG).
1. Important Information Qualification of personnel Designated use • The instrument has been designed so that it is safe to • The Leica TP1020 may be operated only by trained laboratory personnel. All laboratory personnel designated to operate the Leica instrument must read these Instructions for Use carefully and must be familiar with all technical features of the instrument before attempting to operate it.
2. Safety Make sure to comply with the safety instructions and warnings in this chapter. Make sure to read these instructions, even if you are already familiar with the operation and use of other Leica products. 2.1 Safety notes These Instructions for Use includes important information related to the operating safety and maintenance of the instrument.
2. Safety Transport and installation • • • • • Keep in mind Chapter 3, "Technical data"! During transport, keep the instrument upright! When transporting the instrument, do not lift it up by the carousel cover! Do not operate the instrument in rooms with explosion hazard. Caution! The voltage selector is factory preset. Before connecting the instrument to the power supply, make sure this setting is correct for your laboratory's electrical connection values.
2. Safety Working with the instrument (continued) • Always observe worker's protection rules and use adequate protective gear (gloves, laboratory coats). • The heated wax baths may only be used with paraffin. Under no circumstances may they be filled with solvents.
2. Safety Maintenance • Only authorized and qualified service personnel may access the internal components of the instrument for service and repair! • Before replacing the fuses, turn off the power switch and unplug the instrument! • Burnt-out fuses may only be replaced by fuses of the same type and specification. For appropriate brands and specifications, see Chapter 3 "Technical Data". Wax baths • If the excess temperature repeatedly - please call Leica Technical Service.
3. Instrument Components and Specifications 3.1 Technical data Type TP1020 Approvals ������������������������������������������������������������������������������������������������������������������������������������������ UL / cUL / VDE Electrical data Nominal voltage....................................................................................................100 / 120 / 230 /240 V AC ±10 % Rate frequency............................................................................................
3. Instrument Components and Specifications Technical data (continued) Reagent stations Number.................................................................................................... 10 (9 when 3rd wax bath is installed) Capacity............................................................................................................................................................. 1.8 l Tissue basket Quantity:..................................................................................
3. Instrument Components and Specifications 3.2 Description of the instrument The Leica TP1020 is an automatic tissue processor designed for laboratory applications. It is used for the fixation, dehydration and infiltration of histological tissue samples with fixatives, alcohol, solvents and paraffin wax. The reagent stations numbered 1 - 10 are used to contain reagents. Station 10 may be replaced with an optional third wax bath.
3. Instrument Components and Specifications 3.3 Standard delivery—packing list The Leica TP1020 is available in eleven different configurations, which are described below. All variants also include accessories as described in the table on page 17. If the vacuum function or extraction is required for the intended processes to be carried out using this instrument, this information must be included in the original order.
3. Instrument Components and Specifications Standard delivery continued Variant 5 Order No. One basket operation/standard tissue basket. With vacuum/with fume control system. 1 Basic instrument 14 0422 30535 10 Aluminum reagent containers for vacuum 14 0422 42545 10 Reagent container carriers 14 0422 42545 2 Wax baths, (1.8 liters) 14 0422 30665 1 Standard tissue basket 14 0422 30585 1 Tubing band clamp 14 0422 31973 Variant 6 Two basket operation (double-loading)/standard tissue baskets.
3. Instrument Components and Specifications Standard delivery continued Variant 10 One basket operation/standard tissue basket. With fume control system and accessories. 1 Basic instrument 12 Glass beakers with handle 2 Wax baths, (1.
3. Instrument Components and Specifications 3.4 Overview of the Leica TP1020 - with additional wax bath (optional accessory) Carousel cover Reagent station lid Tissue basket holder Glass container with holder Standard tissue basket Wax bath Beaker carrier with fill level marks Notch for wax bath connection cable Container station platform Control panel Fig.
3. Instrument Components and Specifications Overview of the Leica TP1020 - with additional wax bath (optional accessory, continued) Continued 12 11 10 Voltage selector Mains switch Connections for wax baths (connection socket for station 10 with cap (not pictured) closed) Wax bath plug Socket for power cable Fig. 3 - TP1020 - rear side 20 Instructions for Use V 2.
3. Instrument Components and Specifications 3.5 General overview Leica TP1020 - with fume control system Plexiglas fume containment shield Fig.
3. Instrument Components and Specifications General overview Leica TP1020 - with fume control system (continued) Activated carbon filter receptacle Hose clamp Fume outlet tube (optional accessory) Fig. 5 22 Instructions for Use V 2.
3. Instrument Components and Specifications 3.6 Accessories supplied together with the instrument 3.6.1 Standard tissue basket Holding capacity: 100 cassettes Tissue cassettes are loosely arranged in the basket. The maximum capacity is 100 cassettes. The cassettes are stacked in the basket. So that the infiltration process is not disturbed, no cassette may lie on top of the stack. The maximum filling level must be reduced.
3. Instrument Components and Specifications 3.6.3 Aluminum container with holder (instruments with vacuum function only or as an optional accessory) Holding capacity: 1.8 l Instruments equipped with vacuum function are supplied with aluminum containers instead of glass containers. In aluminum containers the level indications marks are located on the inside of the container.
3. Instrument Components and Specifications 3.6.4 Wax bath 5 4 2 1 If the actual temperature rises above the range of normal working temperature, an excess temperature switch-off mechanism responds. The wax bath heating is disconnected. The yellow signal lamp is extinguished. Use of the wax bath can be resumed only after a cooling down period. For cooling down, disconnect the wax bath connector plug from the socket at the rear of the instrument, or switch off the main switch.
4. Initial Operation 4.1 Unpacking instructions When the instrument arrives, check the tilt indicator (Fig. 10) on the package. If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage. If obviously there is damage, please contact your freight forwarder immediately. Fig.
4. Instrument Setup 8 9 12 13 • Remove the wooden ledge (8) sideways from the interior box (9). • Remove the interior box (9) in an upward movement. • To lift up the instrument (using 2 persons), take it by the reagent container platform or by the lower end of the housing (see Fig. 10b,"" "" below) and lift it from the base plate (10). • Install the instrument on a stable laboratory bench. Four rollers (11) under the instrument housing permit rotating the instrument on the bench.
4. Initial Operation Unpacking (continued) When transporting the instrument, do not lift it up by the carousel cover! • To lift up the instrument take it by the reagent container platform or by the lower end of the housing (Fig. 11) and lift it from the base plate (8). 11 • Place the instrument on a stable laboratory table. Four rollers (12) under the instrument housing permit rotating the instrument on the bench.
4. Instrument Setup 4.2 Installation site requirements Because of the quantity of solvents used for processing specimens and the solvent concentrations that build up, we recommend installing instrument versions without a fume control system or active carbon filter in the laboratory's fume hood. The instrument may not be operated in hazardous locations. The installation site has to fulfill the following conditions: • Stable and plane installation surface.
4. Initial Operation 4.3 Electrical connection 4.3.1 Checking the voltage selector setting 230 3 1 2 The voltage selector is located in a shell (3) above the power switch (2) at the rear of the instrument. The actual selected voltage can be seen in the window (1). • Check if the setting showing in the window (1) corresponds to the nominal voltage in your laboratory. • Remove the adhesive tape. If the setting is correct proceed at 4.3.3. 230 V Fig.
4. Instrument Setup 4.3.2 Adjusting the voltage selector When adjusting the voltage selector setting, the instrument must be disconnected from the mains. 5 • Insert the small screwdriver (4) into the smaller one of the two notches at the lower end of the lock (5) using the screwdriver as a lever. 4 • Remove the shell (3). 2 • Remove the voltage selector (6) from the shell (3) and reinsert it so that the correct voltage selection can be seen in the window (1) of the shell.
4. Initial Operation 4.3.3 Connecting the power cable The instrument is supplied together with various country-specific power cables. The instrument may only be connected to the power with the cable supplied together with the instrument and it may only be connected to grounded sockets. 230 • Before connecting the power cable, check if the power switch (2) at the rear of the instrument is disconnected ("O").
4. Instrument Setup 4.4 • Turn on the instrument with the power switch on the right side at the rear of the instrument. Switching on the instrument The instrument does a startup run. Next, the instrument name and software version (e.g. V 1.00) are displayed. This reading disappears after about 10 seconds. Next, the number of the processing station where the basket holder is located and the real time indication are displayed. 4.5 • Press "CLOCK".
4. Initial Operation 4.6 Installing the accessories • Press ARROW UP to lift the carousel. 1 To install and remove the reagent containers and wax baths, the instrument can be rotated on the bench. This ensures good access to all stations at any time. 2 3 Fig. 17 4.6.1 Installing the wax baths The Leica TP1020 basic instrument is delivered with two wax baths (no. 11 and 12). Optionally a third wax bath can be connected to station no. 10.
4. Instrument Setup 4.6.2 Connecting the wax baths The connection plugs of each wax bath has to be inserted into the corresponding socket number at the rear of the instrument. • Rotate the instrument so that the sockets are easily accessible. • Insert plug number 12 into socket number 12. 12 • Insert plug number 11 into socket number 11. 11 • Lock clip (1) at each plug. 10 1 Fig. 18 Before connecting a third wax bath to station No. 10, the cover cap has to be removed.
4. Instrument Setup 4.6.3 Fastening the wax bath connection cables (instruments with fume control system only) Instruments with fume control system are equipped with a Plexiglas fume containment shield which consists of two parts. To ensure the fume containment shields can be freely rotated and displaced as required without being obstructed by the wax bath connection cables, two magnetic clamps are provided on each of the cables to attach the cables to the housing.
5. The User Interface Control panel Fig. 20 The control panel is an easy-care key pad. The individual keys are organized in four functional groups. Pressing any key will be acknowledged by an audible signal. This function can be disconnected if required (see Chapter 15). The standard display shows the respective number of the station in which/above which the holder for the specimen basket is currently located. At the same time the display also reads real time (24 hour clock).
5. The User Interface Key functions Programming, control panel locking, time setting, up-and-down movement of the tissue basket After 10 seconds the display returns to standard reading. All key functions with the exception of the locking key itself are now locked. None of the key functions can be activated. When pressing any key, the display will always read "LOCKED" for 10 seconds. • To unlock the key functions, press "KEY" again for 5 seconds.
5. The User Interface "THREE ARROW" button for carousel up and down movement The "THREE ARROW" button is used to start and stop the carousel (tissue basket) up and down movement. When this function is activated, the tissue basket is lifted and lowered in three-second intervals within the processing stations. This ensures a uniform mixing of all liquids and an optimized tissue infiltration. This function is automatically activated when an automatic processing cycle is started.
5. The User Interface Start, pause, resume, stop and abort automatic tissue processing cycles START button To immediately start processing or to insert delayed starting parameters for automatic processing after having selected a program. PAUSE/CONT button To pause and resume an automatic processing cycle. When the function is activated, the display reads "PAUSE". In addition, 5 minutes after last pressing a button, a double sound signal will remind the user that processing is still paused.
5. The User Interface Manual processing keys These buttons are only functional in the manual processing mode a while and, while an automatic processing mode is paused, via the "PAUSE/ CONT" button. While the carousel is moving, the display reads "WAIT!" and two blinking arrows indicate the direction of the movement. Only when the carousel has completely come to a halt, this indication will disappear from the display.
5. The User Interface Programming mode buttons These buttons are used to enter or alter parameters when programming. ARROW LEFT / ARROW RIGHT buttons (cursor) To move the cursor in the direction of the arrow to the respectively next data entry position. These buttons are equipped with a repeat function. If one of the ARROW is pressed for a while, the cursor jumps to the respectively next data entry position.
6. Programming the Instrument 6.1 General description Example of a one-basket-program Station 1 2 3 4 5 6 7 8 9 10 11 12 Reagent Formalin Formalin Alcohol 70 % Alcohol 80 % Alcohol 96 % Alcohol 100 % Alcohol 100 % Alcohol 100 % Xylene Xylene Paraffin Paraffin Leica TP1020 VAC V V V V V V V V V V V V Duration 1h00 1h00 1h30 1h30 1h30 1h00 1h00 1h00 1h30 1h30 2h00 2h00 Programs are created step by step in the programming mode. Programming parameters have to be entered for each station individually.
6. Programming the Instrument 6.1.1 Special characteristics of two-basket programs Example of a two-basket-program Station Reagent VAC Duration 1 2 3 4 5 6 7 8 9 10 11 12 44 Formalin Formalin Alcohol 70 % Alcohol 80 % Alcohol 96 % Alcohol 100 % Alcohol 100 % Xylene Xylene Paraffin Paraffin Paraffin - 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 2h00 In one-basket programs, for every processing station a different infiltration time can be selected.
6. Programming the Instrument 6.2 Setting-up / editing programs 6.2.1 Selecting programming mode • To select the programming mode, push the PROG key. The following parameters are displayed: - Number of baskets or , Number of processing station 1 - 12, Vacuum "ON" or "OFF", Tissue infiltration time in the displayed station "0 h 50 min", - Program number "P1 - P9", - Programming mode "PROG". 6.2.2 Selecting the program The cursor blinks at the program number.
6. Programming the Instrument 6.2.4 Selecting the starting station • Press ARROW RIGHT to move the cursor to the next entry position. The cursor blinks at the station number data entry position. • Select the desired station number by pressing PLUS or MINUS. 6.2.5 Activating the vacuum function (instruments with vacuum function only) • Press ARROW RIGHT to move the cursor to the next entry position. The cursor blinks at the two positions next to the "V" for vacuum function.
6. Programming the Instrument 6.2.6 Selecting infiltration times per station • Press ARROW RIGHT to move the cursor to the next entry position. The cursor blinks at the hour entry digits. • Press PLUS or MINUS respectively to enter the hours. • Press ARROW RIGHT to move the cursor to the next entry position. For stations you wish to leave out of your program, select an infiltration time of 0 h 00 min. The cursor flashes on both digits destined for entering the minutes.
6. Programming the Instrument 6.2.7 Displaying total program duration Once a program is set up, total run time can be displayed. • Press CLOCK to display the total run time of the program. The total duration displayed here is 1 day, 0 hours and 12 minutes. • To quit the display indication, press CLOCK again. While you are in the programming mode, you cannot start a program. Prior to starting a program, you must quit the programming mode. 6.2.
7. Preparations for Tissue Processing 7.1 Filling the reagent stations • Lift the carousel cover. • Fill all stations with the corresponding reagents. Make sure to observe the minimum and maximum level indication marks. Spilled reagents have to be wiped away immediately. In case of long-term exposure, the instrument surfaces are only conditionally resistant to solvents. • Mount every container onto the station holder at the corresponding station.
7. Preparations for Tissue Processing 7.2 Filling the wax baths The heated wax baths may only be used with paraffin. Under no circumstances may they be filled with solvents.
7. Preparations for Tissue Processing Altering the standard working temperature (continued) Do not overfill the wax baths! The level of the liquid paraffin must not exceed the upper fill level mark on the inside of the wax bath. • To fill the wax baths, use wax pellets or paraffin which has already been liquefied.
7. Preparations for Tissue Processing 7.3 Inserting the tissue basket Use caution when lowering the carousel! Keep your fingers out of the space between the container lid and the upper rim of the container! • Fill the tissue cassettes or capsules into the tissue basket. • To hook in the tissue basket(s), the basket holder(s) should not be located over a wax bath. • Lift the carousel in the manual processing mode.
7. Preparations for Tissue Processing Inserting the tissue basket (continued) While the carousel is moving, the display reads "WAIT!" and shows two blinking arrows which indicate the direction of movement in each particular case. When the carousel has completely come to a halt, this indication will disappear from the display. To avoid reagent carryover as far as possible in the manual mode, allow for a sufficient dripping time.
8. Operating the Instrument in the Manual Processing Mode 8.1 Lifting and lowering the tissue basket Use caution when lowering the carousel! Keep your fingers out of the space between the container lid and the upper rim of the container! Pressing the corresponding button, the basket can be lifted out of a station or lowered into one. • To lift the basket, press ARROW UP. • To lower the basket, press ARROW DOWN.
8. Operating the Instrument in the Manual Processing Mode Lifting and lowering the tissue basket (continued) + • To lower the basket press KEY and ARROW DOWN simultaneously. Or + 8.2 • To lift the basket, press KEY and ARROW UP simultaneously. Moving the tissue basket to the next station To transport the tissue basket from one station to the next, the CIRCLE ARROW button has to be pressed. The movement can only be carried out in single steps and clockwise. • Press CIRCLE ARROW.
8. Operating the Instrument in the Manual Processing Mode 8.3 Activating the vacuum function (instruments with vacuum function only) The Leica TP1020 is available with and without a vacuum function. Instruments without vacuum function also have the "V" button for vacuum on the control panel, however this key is not functional. "V" is not displayed. Of course a vacuum function cannot be activated with the "V" buttons in those cases.
9. Operating the Instrument in the Automatic Processing Mode 9.1 Starting a program A program can either be started immediately or at a later point in time using the delay function. 9.1.1 Immediate start All corresponding parameters are displayed, such as number of baskets, starting position (station number), vacuum on/off, and infiltration time of the basket in the starting container. Therefore, check the parameters on display and decide if you actually wish to start the displayed program.
9. Operating the Instrument in the Automatic Processing Mode Immediate start (continued) • To start the selected program immediately, press START again. The warning codes W:04 - W:06 (see Chapter 13) are displayed for programs of less than 8 hours duration from start until the basket reaches the first wax bath. Check if the available amount of time is sufficient for the paraffin to liquefy completely. If this is not ensured, fill the wax baths with liquid paraffin.
9. Operating the Instrument in the Automatic Processing Mode Immediate start (continued) From that point on, the remaining infiltration time of the basket in the current station is displayed. While processing time is running, the display reading is updated in one-minute intervals; i.e. you can always see exactly the remaining infiltration time for each station. 9.1.2 Delayed start Select starting time • Press START. The program which was used last is displayed.
9. Operating the Instrument in the Automatic Processing Mode Delayed start (continued) • Press PLUS / MINUS to enter the number of days. • Press ARROW to move the cursor to the data entry position for the hours. • Press PLUS / MINUS to enter the number of hours. • To move the cursor to the digits destined for entering the minutes, push the ARROW key. • Press PLUS / MINUS to enter the number of minutes. The program shown here would be started the next day at 7.30 PM.
9. Operating the Instrument in the Automatic Processing Mode Delayed start (continued) Edit and change starting time (when using the delayed starting function) • Press CLOCK again. • Check the selected starting time and if necessary change it as appropriate to achieve the desired end or run time. • To activate the delay function press START. The warning codes W:04 - W:06 (see Chapter 7) are displayed for programs of less than 8 hours duration from start until the basket reaches the first wax bath.
9. Operating the Instrument in the Automatic Processing Mode Locking the key functions To protect program settings against unintended deletion or alterations, the key functions of the control panel can be locked. • To lock the panel, press KEY for 5 seconds. "LOCKED" will be displayed. After 10 seconds the display will return to standard reading. Every time a button is pressed, "LOCKED" will be displayed again. • To unlock the control panel functions, press KEY again for 5 seconds.
9. Operating the Instrument in the Automatic Processing Mode 9.2 Editing and modifying programs while a processing cycle is in progress Programs can be edited and changed while a processing cycle is in progress. All programs can be edited and changed with the exception of the program currently in progress. A program in progress can be displayed but not changed. • Activate the programming mode. • Select a program.
9. Operating the Instrument in the Automatic Processing Mode Editing and modifying programs while a processing cycle is in progress (continued) In the example shown here end of processing would be the next day at 10.38 AM. • To quit the display indication, press CLOCK again. 9.4 Pausing a process Automatic specimen processing can be interrupted and resumed later, for example to reload specimens. • To interrupt the program, press the PAUSE/ CONT button. "PAUSE" is displayed.
9. Operating the Instrument in the Automatic Processing Mode 9.5 Resuming a paused process • To reassume processing press PAUSE/CONT again. Pressing this key will lower the basket into the station. Processing continues as programmed. While a run is paused, the infiltration time countdown in the actual station will also be interrupted. When processing is resumed, the remaining infiltration time in the station will be carried out without interruption.
9. Operating the Instrument in the Automatic Processing Mode Stopping or terminating a process (continued) If there is an emergency, an automatic processing cycle can be stopped immediately and - in a subsequent step - entirely aborted. Processing will continue as programmed. • To abort the process, press STOP again. This will terminate the specimen processing definitely without any option to resume processing. 9.
9. Operating the Instrument in the Automatic Processing Mode 9.8 Remove the specimens • Lift the carousel. • Allow for the tissue basket to drain in that position. • Lift the tissue basket slightly with your hand and pull it out of the basket holder in a horizontal movement. • Lower the carousel. 9.9 Finishing your daily work • Check the filling level and quality of the liquid in each station (reagent and wax baths) and refill or exchange completely if necessary.
10. Warning and Error Codes – Troubleshooting The warning codes W:01 - W:06 may be displayed. 10.1 Warning codes Solid paraffin takes several hours to melt. Please keep this in mind and make sure to switch on the instrument early enough so the paraffin will be molten and ready for use when necessary. + Or W:01, W:02, W 03 - paraffin in station No. 10, 11, 12 is still solid. W:04, W:05, W:06 - paraffin in station No. 10, 11, 12 may still be solid when basket No. 1 arrives.
10. Warning and Error Codes – Troubleshooting Warning code (continued) W:01 to W:03 block transportation of the tissue basket into or out of the wax bath of station 10, 11 or 12. However, if you are certain that the paraffin is actually liquefied, you can manually immerse the tissue basket into or remove it from the wax bath. + Code Possible root cause • To override the warning codes W:04, W:05 and W:06 press KEY and START simultaneously to start processing.
10. Warning and Error Codes – Troubleshooting To indicate a series of malfunctions, the error codes E:01 to E:13 are displayed. 10.2 Error codes Three consecutive and very short sound signals that are repeated every 10 seconds reinforce the visual error code. On the next page you will find a complete list of all error messages, their meaning and how to troubleshoot them. • To quit the error codes, press any key.
10. Warning and Error Codes – Troubleshooting List of error codes (continued) Error code "E:11" Loss of process data. - Acknowledge the error message by pressing any key; "ABORT" message, the process is canceled - Restart processing cycle. - If the same problem recurs, call Technical Service. Error code "E:12" Loss of program settings. - Acknowledge the error message by pressing any key; "ABORT" message, the process is canceled - Restart processing cycle.
10. Warning and Error Codes – Troubleshooting 10.3 "POWER FAILURE" and "WRONG STATION" messages In case of a power failure the carousel is lowered immediately into the station where the tissue basket happens to be located when the power failure occurs. Once the power is restored, processing will be resumed as programmed. Possible impairment of the processing cycle through the interruption is displayed.
10. Warning and Error Codes – Troubleshooting 10.4 Measures to remove specimen material in case of prolonged power failure In case of a prolonged power failure the tissue can be removed from the instrument manually and also be relocated to the next station by entirely manual operation. • Switch the instrument off at the main power switch. • Remove the crank handle (1) from the clip and insert it into the access port (2) on the left side of the instrument.
10. Warning and Error Codes – Troubleshooting Warning and Error Codes - Troubleshooting (continued) Use caution when lowering the carousel! Keep your fingers out of the space between the container lid and the upper rim of the container! When you release the crank handle, the carousel will start to slowly descend. Manual relocation to the next station • Rotating the crank handle in the direction of the arrow, lift the carousel upwards until there is a notable resistance to the rotating movement.
10. Warning and Error Codes – Troubleshooting 10.5 "ABORT" message "ABORT" is displayed after quitting an error code emitted due to instrument malfunction. When confirming such an error message processing is aborted. At this point processing can be restarted from the beginning. To skip the stations that had already been covered during the previous aborted run proceed as follows: • Press START. • To start the currently displayed program, press START again.
11. Cleaning • Lift the carousel in the manual processing mode. • Remove all station containers from the platform. Spilled reagents have to be wiped away immediately. In case of long-term exposure, the instrument surfaces are only conditionally resistant to solvents.
11. Cleaning When cleaning the instrument, no liquid may enter in contact with any of the electrical connections or the interior of the instrument. • Use a moist cleaning cloth to clean platform, lacquered instrument surfaces and control panel. • Clean the rims of the glass or aluminum reagent containers and of the wax baths. The glass and aluminum containers are dishwasher-proof. • Take the glass or aluminum containers out of the beaker carriers and wash them in the dishwasher.
12. Maintenance 12.1 General maintenance instructions Only Leica service technicians are authorized to open the instrument for maintenance and repair work. Overall, the instrument can be considered maintenance-free. To ensure its trouble-free functioning over a prolonged period of time, we still recommend: • Have a preventive maintenance done once a year by a service engineer authorized by Leica. • Enter into a service contract at the end of the warranty period.
12. Maintenance 12.2 Wax bath 12.2.1 Altering the standard working temperature Factory-set standard working temperature is 65 °C. Wax bath heating will automatically switch on when the actual temperature of the paraffin is 5 °C below set working temperature. When working with paraffin that has a melting point below 58 °C, the instrument working temperature can be readjusted with the corresponding setting screw. • Use a screw driver to turn the setting screw (1) to the desired value.
12. Maintenance 12.2.2 Reset after excess temperature shutdown If the actual temperature rises above the range of normal working temperature, an excess temperature switch-off mechanism responds. The wax bath heating is disconnected. The yellow signal lamp is extinguished. Use of the wax bath can be resumed only after a cooling down period. For cooling down, disconnect the wax bath connector plug from the socket at the rear of the instrument, or switch off the main switch.
12. Maintenance 12.3 Replacing the fuses Turn the instrument off and disconnect the power plug before replacing the fuses! Burnt-out fuses may only be replaced by fuses of the same type and specification. See Chapter 3 "Technical Data". 7 6 3 1 Fig. 25 The fuses are located in the voltage selector shell (3). The currently selected voltage can be seen in the small window (1) in the voltage selector shell. • Remove the shell (3) as described in Chapter 4.3.2 "Adjusting the voltage selector".
13. Optional accessories 13.1 Three-level tissue basket Fig. 26 Holding capacity: 20 cassettes per level The three-level tissue basket consists of a holder (1) in which the three levels (2) for the cassettes are stacked. The upper level is closed with a lid (3). 82 Instructions for Use V 2.
13. Optional Accessories 13.2 Basket removal device 2 3 The basket removal device with drip tray makes it easy to remove the hot dripping tissue basket at the end of the processing cycle. The rubber insert (4) of the drip tray can be removed for cleaning. • Grasp the basket removal device at the black handle and insert the dripping tray under the tissue basket. • Insert the metal hook (1) under the notch at the bottom of the tissue basket.
13. Optional accessories 13.3 Basket holder for second tissue basket To increase the specimen throughput the instrument can be retrofitted with a basket holder for a second tissue basket. A second tissue basket (standard or three-level type) has to be ordered separately. Standard delivery 1 basket holder for second tissue basket 1 wax bath 1 station holder for wax bath 1 2 3 4 Fig. 28 84 Instructions for Use V 2.
13. Optional Accessories Basket holder for second tissue basket (continued) 8a/8b 7 4 5 6 Fig. 29 Installing the second basket holder • Rotate the carousel cover counterclockwise until it can be removed from the axle (3) in an upward movement. The second basket holder is installed at position 2. For this purpose, the existing cover and bar must be removed. • Clutch the rod (5) with a pair of tongs and release the slotted screw (8a) on the upper side of the disc (4).
13. Optional accessories Basket holder for second tissue basket (continued) The instrument standard configuration will then have to be changed accordingly to acknowledge that a third wax bath has been connected - see Chapter 15. 12 11 10 2 1 Fig. 30 Installing the station holder Connecting the third wax bath • Use a screw driver to lift the reagent station holder out of the platform. • Pull off the cover cap from the socket no. 10. • Insert the wax bath holder and drive in with a plastic hammer.
13. Optional Accessories 13.4 Connecting the fume outlet tube (optional accessory for instruments with fume control system) Via a solvent resistant fume outlet tube (which is available in a length of 2 or 4 meters), the instrument can be connected to a central fume extraction system. The fume outlet tube can also be used to conduct solvent fumes outside a building. 2 1 Fig.
13. Optional accessories 13.5 Inserting the activated carbon filters (optional on instruments with fume control system) Instruments with fume control system can be equipped with two activated carbon filters. One filter is specific to formaldehyde, whilst the other takes up other solvents. The working life of the activated carbon filters is dependent on the reagent concentrations actually used, instrument running time and ambient temperature. • Unpack the filters.
14. Ordering Information 14.1 Accessories Standard tissue basket....................................................................................................... 14 0422 30585 Three-level tissue basket................................................................................................... 14 0422 30547 Single-level compartment for three-level tissue basket.............................................. 14 0422 30622 Lid for three-level tissue basket.........................................
15. Appendix 15.1 Modifying the instrument configuration The instrument has a factory-set standard con- figuration which can be modified by the user if desired. Modifications can be done in the configuration menu, which consists of 4 different menu items: - Signal level 1 - 4 - 3. Wax bath? - ProgPreset? - SystemReset? + For this menu item four different settings can be selected. Setting 4 is factory preset. Setting 1 is the lowest level.
15. Appendix Modifying the instrument configuration (continued) • For the next menu item, press the CURSOR button. • To change the setting, press the PLUS / MINUS keys. The next standard setting is displayed. 3. Wax bath = do you wish to use a third wax bath? In station no. 10 you can install a third wax bath instead of the reagent station. If you do so, you have to modify the configuration menu accordingly; otherwise the instrument will not function properly.
15. Appendix Modifying the instrument configuration (continued) Programs no. 6 - 9 come with a number of factory preset standard values; however these may be altered by the user. Standard setting is "NO". • To change the setting, press the PLUS key. When answering "YES", programs no. 6 - 9 are set to standard values and programs no. 1 - 5 are deleted. • For the next menu item, press the CURSOR button. A system reset will set real time to 0.00 and maximum heating time for the wax baths to 8 hours.
15. Appendix 15.2 Reagents appropriate for use with the instrument The following reagents are safe for use with the TP1020: Important! Using other than the reagents listed below can cause damage to the instrument or to parts of the instrument.
15. Appendix 15.3 Factory-set programs Program No. 6 - 1 basket Station 1 2 3 4 5 6 7 8 9 10 11 12 Reagent VAC Duration Paraffin Paraffin V V V V V V V V V V V V 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 VAC Duration V V V V V V V V V V V 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 0h15 Program No. 8 - 2 baskets Station Reagent 2 3 4 5 6 7 8 9 10 11 Paraffin 12 Paraffin 94 Instructions for Use V 2.
15. Appendix 15.4 Factory-set programs (continued) Program No. 7 - 1 basket Station Reagent 1 2 3 4 5 6 7 8 9 10 11 Paraffin 12 Paraffin Program No.
15. Appendix Leica TP1020 - Programming Worksheet Program No. Station Reagent Name: VAC Date: Written by: Duration Notes 1 2 3 4 5 6 7 8 9 10 96 11 Paraffin 12 Paraffin Instructions for Use V 2.
15. Appendix Leica TP1020 - Programming Worksheet Program No.
16. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
Decontamination Certificate17. Decontamination Certificate Dear Customer, Any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems for repair.
** Such equipment must not be returned without the written agreement of Leica Biosystems. 17. Yes No The equipment has been prepared to ensure safe handling/transportation. Decontamination Certificate Whenever possible, please use the original transportation case/box. 3 Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging, or hand it over to the service engineer.
www.LeicaBiosystems.com Leica Biosystems Nussloch GmbH Heidelberger Strasse 17-19 D- 69226 Nussloch Phone: +49 - (0) 62 24 - 143 0 Fax: +49 - (0) 62 24 - 143 268 Web: www.LeicaBiosystems.
