Lorem Ipsum Leica ES2 Leica EZ4 Leica EZ4 HD Manual
Congratulations! You have just purchased a Leica E Series stereomicroscope - an excellent choice! The Leica E series is perfectly suited for practical instruction in schools and universities as well as for routine tasks in the laboratory and industrial production and testing. Viewing entire objects under magnification imparts perspectives and discoveries that would not be possible with the naked eye.
Chapter Overview Safety and Use 6 Leica ES2 10 Leica EZ4 and Leica EZ4 HD 18 Eyepieces (only for Leica EZ4) 29 Photography Using the Leica EZ4 HD 37 The Camera Menu 51 Care and Transport 62 Specifications 64 Dimensions 67 Leica E Series Manual 3
Table of Contents Leica ES2 Overview: Leica ES2 Transport, Placement and Storage The Correct Interpupillary Distance Using the LED illuminator Focusing Magnification Display Adjusting the Resistance of the Focus Drive 11 12 13 14 15 16 17 Eyepieces (only for Leica EZ4) Changing Eyepieces (Only for Leica EZ4, Open Version) Eyepieces and Eyecups (Only for Leica EZ4, Open Version) Dioptric Correction (Only for Leica EZ4, Open Version) Graticules (Only for Leica EZ4, Open Version) Graticules: Pre
Contents (Continued) The Camera Menu Optional Remote Control Viewing Images and Movies with the Optional Remote Control Calling up the Camera Menu COLOR (Automatic White Balance) COLOR (Manual White Balance) EXPOSURE RESOLUTION SETUP CAMERA (Camera Settings) SETUP USER (User-defined Settings) Predefined Illumination Scenarios 52 53 54 55 56 57 58 59 60 61 Care and Transport Care and Transport 63 Specifications Camera Module 65 Dimensions Leica ES2 Leica EZ4 HD Leica EZ4, 10× Eyepieces
Safety and Use Leica E Series Manual 6
Safety Notes Before installing, operating or using the instrument, it is mandatory to read this user manual. In particular, please observe all safety instructions. The "Safety Concept" brochure contains additional safety information regarding service work, requirements and the handling of the microscope, accessories as well as general safety instructions. You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier.
Instructions for Use Intended use The E Series microscopes are optical instruments for improving the visibility of objects through magnification and illumination. It is used for observing and documenting. Interference Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
Instructions for Use (Continued) Intervention from the user The microscopes of the E Series contain no components that can be maintained or repaired by the user. Exceptions are mentioned specifically in this manual. Unless otherwise specified in this manual, repair and service work may be carried out by authorized Leica technicians only. Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims.
Leica ES2 Leica E Series Manual 10
Overview: Leica ES2 1 2 3 1 2 3 4 5 6 7 10× fixed eyepieces for eyeglass wearers Carrying handle Two-level magnification changer 10× and 30× Focusing drive Integrated LED incident light illumination Integrated LED transmitted illumination Switch for incident and transmitted light 4 5 6 7 Leica E Series Manual 11
Transport, Placement and Storage Built-in carrying device All Leica E Series microscopes are equipped with a carrying handle for convenient and safe transport. Leica E Series Setting up the microscope 1. Place the microscope on a flat tabletop. 2. Connect the power supply to a grounded socket. Manual Packing the device away 1. After use, wind up the cable as shown in the illustration. 2. Store the instrument so that it is protected against dust.
The Correct Interpupillary Distance The Ideal Image The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. ✗ Note for eyeglass wearers If you wear glasses, fold the eyecups back – otherwise, fold the eyecups forwards. Setting the eye distance 1. Look into the eyepieces. 2. Hold the eyepieces with both hands. Push the eyepieces together or separate them until you see a circular image.
Using the LED illuminator Iluminating the specimen 1. Position a specimen in the middle of the baseplate. Tips on use ϘϘ Use incident light for plastic, opaque objects. 2. Switch the two LED illuminators on or off as desired. ϘϘ Use transmitted light for transmitted light specimens or transparent objects. ϘϘ Use the combination of both LED illuminators for partially transparent objects. After switching off, the LEDs remain lit for about another 4 seconds.
Focusing Focusing raises and lowers the stereomicroscope using the focusing drive. The specimen is brought into sharp focus as soon as it is in the focal point of the objective. Focusing 1. Set the magnification changer to the lowest level (10). 3. Set the magnification changer to the second level (30). 4. Use the focusing drive once again for fine focus. The focusing drive can be operated either leftor right-handed.
Magnification Display You can read the configured magnification on the right-hand rotary knob of the Leica ES2. The additional magnification provided by the eyepieces is already incorporated in the scale.
Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too easy or too difficult to adjust? No problem - the resistance can be adjusted individually depending on your personal preferences: 1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Leica EZ4 and Leica EZ4 HD Leica E Series Manual 18
Overview: Leica EZ4 1 2 3 1 2 3 4 5 6 7 10x fixed eyepieces for eyeglass wearers Carrying handle Magnification changer Focusing drive Integrated LED incident light illumination Illumination control Integrated LED transmitted illumination 4 5 6 7 Leica E Series Manual 19
Instrument variant: Leica EZ4 HD 1 2 3 4 5 6 1 2 3 4 5 6 7 8 9 10x fixed eyepieces for eyeglass wearers Integrated digital camera with SD card slot Carrying handle Magnification changer IR receiver for optional remote control Focusing drive Integrated LED incident light illumination Illumination control Integrated LED transmitted illumination 7 8 9 Leica E Series Manual 20
Transport, Placement and Storage Built-in carrying device All Leica E Series microscopes are equipped with a holder for convenient and safe transport. Leica E Series Setting up the microscope 1. Place the microscope on a flat tabletop. 2. Connect the power supply to a grounded socket. Manual Packing the device away 1. After use, wind up the cable as shown in the illustration. 2. Store the instrument so that it is protected against dust.
The Correct Interpupillary Distance The Ideal Image The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. ✗ Note for eyeglass wearers If you wear glasses, fold the eyecups back – otherwise, fold the eyecups forwards. Setting the eye distance 1. Look into the eyepieces. 2. Hold the eyepieces with both hands. Push the eyepieces together or separate them until you see a circular image.
Switching on the LED Illuminator Automatic shutoff If the illumination is not operated for 60 minutes, it switches off automatically. The illumination can be reactivated by pressing a button on the membrane keypad. LED illumination 1. Position a specimen in the middle of the baseplate. Leica E Series 2. Switch the two LED illuminators on or off as desired: ϘϘ Use incident light for plastic, opaque objects ϘϘ Use transmitted light for transmitted light specimens or transparent objects.
Controlling the Automatic Shutoff Automatic shutoff The factory settings of the EZ4 models switch the instrument off automatically after one hour without user interaction. This automatic shutoff helps to conserve the lightemitting diodes and save energy. Leica E Series Disabling the automatic shutoff To disable the automatic shutoff, follow these steps: 1. Switch the microscope off. Enabling the automatic shutoff To re-enable the automatic shutoff, follow these steps: 1. Switch the microscope off. 2.
Illumination types Three-level incident light illumination The incident light illumination is made up of 5 LEDs. Two, three or five diodes are switched on depending on the level. Accordingly, the angle of incidence of the light beam on the object is switched from high to low. Press 1× Five diodes: greatest brightness for incident light objects Leica E Series Regulating brightness The brightness is adjusted using the keys and affects all switched-on LEDs.
Focusing Focusing raises and lowers the stereomicroscope using the focusing drive. The specimen is brought into sharp focus as soon as it is in the focal point of the objective. Focusing 1. Set the magnification changer to the lowest level (8). 3. Set the magnification changer to the second level (35). 4. Use the focusing drive once again for fine focus. The focusing drive can be operated either leftor right-handed.
Magnification Display You can read the configured magnification on the right-hand rotary knob of the Leica EZ4 models. The additional magnification provided by the eyepieces is already incorporated in the scale Leica EZ4 models with open tubes On the EZ4 model without eyepieces, only the magnification factors of the magnification changer are indicated: 0.8, 1, 1.25, 1.6, 2, 2.5, 3, 3.5. Different total magnifications are possible depending on which eyepieces are used with the EZ4.
Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too easy or too difficult to adjust? No problem - the resistance can be adjusted individually depending on your personal preferences: 1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Eyepieces (only for Leica EZ4) Leica E Series Manual 29
Changing Eyepieces (Only for Leica EZ4, Open Version) Changing eyepieces Various eyepieces are available for the Leica EZ4, allowing the stereomicroscope to be adapted even better to the specific requirements. The following eyepieces are available for selection: ϘϘ Eyepieces replaceable, fixed or adjustable: 10×/20, 16×/16 or 20×/12 ϘϘ Eyepieces for eyeglass wearers 10×/20B and 16×/15B, fixed and adjustable Adjustable eyepieces for eyeglasses with straight eyecups.
Eyepieces and Eyecups (Only for Leica EZ4, Open Version) Eyecups The eyecups can be replaced. Risk of infection Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be minimized by the use of individual eyecups. Eyeglass eyepiece with adjustable diopter and straight eyecups Leica E Series Manual Eyepiece for users who do not wear eyeglasses with adjustable diopter and inclined eyecup Fixed eyepiece with inclined eyecup.
Dioptric Correction (Only for Leica EZ4, Open Version) Dioptric Correction The adjustable eyepieces for the Leica EZ4 are also available with built-in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises a maximum of ±5 diopter settings. Users with normal vision do not need adjustable eyepieces. An adjustable eyepiece is sufficient for mild shortsightedness. Adjusting 1. Set the dioptric correction for both eyepieces to "0".
Dioptric Correction (Continued) 7. Look into the eyepiece. 8. Slowly rotate the eyepiece individually in the "–" direction until the eye sees the object sharply imaged. 9. Select the highest magnification and refocus if necessary. Now, if you adjust the magnification from the highest to the lowest level, the specimen is always brought into sharp focus. If not, repeat the process.
Graticules (Only for Leica EZ4, Open Version) Graticules The transparent graticules can be inserted in the adjustable eyepieces of the Leica EZ4. They enable easy measurements at the specimen. Available eyepiece inserts Graticules 10 mm/ 0.1 mm 5 mm/ 0.1 mm 5 mm/ 0.05 mm 100 Div / 0.002" For equipment with a graticule in one eyepiece, two adjustable eyepieces are needed.
Graticules: Preparation Care instructions The graticule must always be clean and dust-free! Never touch the graticule with bare hands! Always hold the graticule by the edges! Leica E Series Variant 1: 10× and 20× eyepieces The 10× and 20× adjustable eyepieces include an insert at the bottom with a snap mount. Variant 2: 16× eyepieces On the 16× adjustable eyepieces, the insert is fixed to the eyepiece by a screwed connection. 1.
Inserting the Graticules Inserting the graticules is the same for all eyepiece types. 4. Put the insert back into the eyepiece. Depending on the variant, you have to either press or twist the insert into place. 6. Align the graticules by turning the eyepiece in the tube. 1. Place the insert with the knurled side on the work surface. 2. Grasp the graticule so that you see the scale in laterally correct form. 3.
Photography Using the Leica EZ4 HD Leica E Series Manual 37
Leica EZ4 HD: Overview of the instrument 1 1 2 3 2 4 1 2 3 4 3 SD card slot Status LED Black multifunction button Red multifunction button Leica E Series 1 2 3 Manual USB connection Pinhole switch HDMI connection 38
General Instructions General Instructions ϘϘ The software for control is included on the DVD provided with the instrument. ϘϘ Install the software before connecting the Leica EZ4 HD to the PC or Mac. ϘϘ As soon as the EZ4 HD is connected to a power cable and the main switch is turned on, the integrated camera is also ready for use. ϘϘ If a USB cable is not connected and there is no SD card in the card slot of the Leica EZ4 HD, the status LED of the instrument lights up RED.
Introduction The Leica EZ4 HD puts some extremely interesting additional functions at your fingertips. The integrated digital camera is designed for the sole purpose of giving you the best possible quality for your documentation – with particularly easy handling.
Standard delivery DVD with Leica software, drivers and manuals USB cable HDMI cable SD card Optional: Remote control with battery (Type CR2025) Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise.
HD mode and PC mode The camera is normally in what is known as "HD mode" after being switched on. In this mode, it outputs a high-resolution signal in 16:9 widescreen format. The images can then be saved directly to an SD card. If the Leica EZ4 HD is connected to a computer via USB and the Leica software provided is started, the camera switches automatically to what is called "PC mode". In this mode, images are transmitted to the computer with a size of up to 3 megapixels. The aspect ratio is always 4:3.
Capturing Images without a Computer in HD Mode Saving to SD card Images can be saved directly to an inserted SD card. The card has to be formatted using the "FAT" file system. When the SD memory card is full, the LED on the camera is illuminated in red. No more images can be captured. Leica E Series Changing between PC and HD mode In order to take photos without a computer you must be in HD mode (not PC mode). After the camera is switched on, it is in the last selected mode.
Capturing Images without a Computer in HD Mode (Continued) Capturing an image 1. Insert the SD memory card into the SD card slot until you hear it click into position. 2. Press the red image capture button briefly. You hear an audible signal as confirmation and the camera LED flashes green. The LED illuminates in green. The camera is now ready to save images to the SD card. You will hear another beep when the Leica ICC50 HD has completed capturing the image. The image is now saved on the SD card.
High-resolution Video Display without a Computer (HD mode) Cables 1. Connect the camera and the HDMI device via the integrated HDMI port. The HDMI cable required for this purpose is included in the standard delivery of the Leica EZ4 HD. Changing the Resolution The output resolution (Full HD or HD ready) is configured directly on the microscope. Live images and captured images can be output in high resolution via the HDMI 1. Press a pin into the pinhole switch in order to change the resolution.
PC Mode: Use with a Computer For instructions on operating the instrument in PC mode, refer to the DVD included in the standard delivery of the Leica EZ4 HD. The 10× eyepieces of the Leica EZ4 HD have a field of view of 20 mm. Unlike the circular object field through the eyepieces, the image section of the camera is rectangular and has a diagonal measurement of 16.4 mm. Therefore, the image field of the camera is smaller than the visible object field through the eyepieces. 3.
PC Mode: Limitations Aspect ratio If the Leica EZ4 HD is connected to a computer and is in PC mode, images are always output in a 4:3 size ratio and in a lower resolution – even if a 16:9 monitor or television set is connected via HDMI. The image is necessarily compressed on such a device. In this case, we recommend that you not connect the television to the microscope, but to the computer as a second monitor.
PC Mode: Additional Use of a HDMI Device In PC mode, the camera image is always shown in the preview window of the Leica software. In addition, another computer monitor or television can be connected via the HDMI interface. The image resolution on the attached HDMI or television screen is limited to the live image resolution inside the LAS EZ software. Due to technical constraints, it is not possible to send a live image stream to the connected screen in "HD ready" or "Full HD" quality.
Configuring the White Balance W When configuring the white balance of the camera, make sure that no specimen is in the beam path and that a standard illumination is available. 5 sec. Configuring a New White Balance Press and hold the red exposure button for 5 seconds. Wait for the beep. As soon as the signal sounds, a new white balance has been configured. Leica E Series Manual e recommend carrying out a new white balance adjustment whenever you change the illumination type or color temperature.
Restoring Factory Settings Factory settings 1. Press the red multifunction button for 10 seconds to delete all individual settings and reset the instrument to the factory settings. 10 sec.
The Camera Menu If you purchased the optional remote, you will have access to the camera menu.
Optional Remote Control The remote control does not work if the camera is in PC mode. 1. 2. 3. 4. 1 2 3 4 5 6 5. 6. 7. 8. 9.
Viewing Images and Movies with the Optional Remote Control If you have purchased the optional remote control, you can display the images and videos captured by the HD camera directly on a HD screen. 1. Miniature view: 3. To return to live view press any button on the camera or press the key 1, 2 or 9 on the remote control (see previous page). 007/0109 ICC500007.MP4 (press 1×) ϘϘ Select miniature: , , and ϘϘ Show full-size image or play back video: Playback / fullscreen view 2.
Calling up the Camera Menu The remote control does not work if the camera is connected to a computer and is in PC mode. The menus of the camera are also no longer displayed on the monitor. In this case, press the black multifunction button for 5 seconds to switch to HD mode (refer also to page 42). Opening and closing the camera menu 1. Point the remote control towards the microscope. 2. Press the ▤ key on the remote control to show the camera menus on the monitor. 3.
COLOR (Automatic White Balance) COLOR SET WHITE BAL PRESS OK EXPOSURE WB MODE AUTO RESOLUTION RED LEVEL SETUP CAMERA BLUE LEVEL SETUP USER BLACK LEVEL MAIN MENU Enabling automatic white balance 1. Press the ▤ button on the remote control. 2. Call up the "COLOR" entry. 3. Set the value for "WB MODE" to "AUTO". 4. Press the ▤ button to exit the menu. SUBMENU The "COLOR" function makes it possible to adapt the camera to the ambient light so that color-neutral images can be acquired.
COLOR (Manual White Balance) COLOR SET WHITE BAL PRESS OK EXPOSURE WB MODE MANUAL RESOLUTION RED LEVEL SETUP CAMERA BLUE LEVEL SETUP USER BLACK LEVEL MAIN MENU Adjusting the white balance manually (recommended) 1. Move the specimen out of the field of view so that only the illumination is seen. 2. Press the ▤ button on the remote control. 3. Call up the "COLOR" entry. SUBMENU 4. Select the "SET WHITE BAL" menu item and press the "OK" button.
EXPOSURE COLOR EXP MODE EXPOSURE EXPOSURE RESOLUTION GAIN SETUP CAMERA BRIGHTNESS SETUP USER GAMMA MAIN MENU EXP MODE EXPOSURE EXPOSURE RESOLUTION GAIN SETUP CAMERA BRIGHTNESS SETUP USER GAMMA Leica E Series Submenu Manual exposure 1. Press the ▤ button on the remote control. 2. Call up the command for manual exposure (see diagram at the left). 3. Set the value for "EXP MODE" to "MANUAL". 4. Correct the values for "EXPOSURE", "GAIN" and "GAMMA" until you obtain the desired results.
RESOLUTION COLOR LIVE 1280X1080 EXPOSURE CAPTURED 2048X1536 RESOLUTION MOVIE 720X480 SETUP CAMERA QUALITY HIGH SETUP USER SHARPNESS HIGH MAIN MENU CAPTURED (resolution of the captured image) Defines the resolution of the captured image if the Leica EZ4 HD is not connected to a computer. The following resolutions are available: 640×480, 1024×768, 1600×1200 and 2048×1536.
SETUP CAMERA (Camera Settings) COLOR SET DATE TIME PRESS OK EXPOSURE RESET CAMERA PRESS OK RESOLUTION FLIP IMAGE 0° SETUP CAMERA OVERLAY NONE SETUP USER CAM MODE PC-MODE Main menu RESET CAMERA This command resets the camera to the factory settings. All user information (such as white balance, resolution etc.) is reset. FLIP IMAGE Rotates the live image by 180°.
SETUP USER (User-defined Settings) COLOR SET ILLUM ILLUM-1 EXPOSURE MENUCOLOR LEICA/LAS RESOLUTION SHOW MENU 15 SEC SETUP CAMERA SHOW CAPTURE 2 SEC SETUP USER LANGUAGE ENGLISH Main menu SET MENUCOLOR Currently, two color schemes are available for the menu guidance. Additional colors may be provided in the future via a firmware update. SHOW CAPTURE Defines how long an image is displayed on the screen after being captured.
Predefined Illumination Scenarios The camera in the Leica EZ4 HD provides various illumination modes that cover a wide variety of possible situations. 2. Press the button again to switch to the next illumination mode. 3. Press the red image capture button or wait until the menu no longer appears. Changing the illumination mode 1. Press the black multifunction button briefly to display the current illumination mode on the monitor or TV set.
Care and Transport Leica E Series Manual 62
Care and Transport General Instructions ϘϘ Keeping all optical components clean is important for maintaining good optical performance. ϘϘ ϘϘ ϘϘ Always protect the microscope and camera with the plastic cover when the instrument is not in use. (Included in the delivery package of the microscope.) Be sure power to the camera is off when the dust cover is in place.
Specifications Leica E Series Manual 64
Camera Module Camera Weight 700 g (camera only) Height 50mm Exposure time 2 msec – 2 sec Live image Max. 45 fps (1256 × 720 px) – 20 fps (1920 ×1080 px) Full frame image acquisition 2048 × 1536 pixels, 3.1 megapixels Movie clip 720 × 480 pixels (MP4) Sensor size 6.55 mm × 4.92 mm Pixel size 3.2 μm × 3.
Camera Module (Continued) Mechanical and optical interfaces ϘϘ Integrated camera module Other ϘϘ Power supply: integrated power supply ϘϘ Light distribution Optical 50% / Camera 50% ϘϘ Power requirement: 4 W ϘϘ Internal video magnification 0.5× ϘϘ Operating temperature range +5 °C to 50 °C ϘϘ Sensor Centered to optical axis ± 0.
Dimensions Leica E Series Manual 67
Leica ES2 Leica E Series Manual 68
Leica ES2 (Continued) Leica E Series Manual 69
Leica EZ4 HD Leica E Series Manual 70
Leica EZ4 HD (Continued) Leica E Series Manual 71
Leica EZ4, 10× Eyepieces Leica E Series Manual 72
Leica EZ4, 10× Eyepieces (Continued) Leica E Series Manual 73
Leica EZ4 without Eyepieces Leica E Series Manual 74
Leica EZ4, without Eyepieces (Continued) Leica E Series Manual 75