Leica ES2 Leica EZ4 Leica EZ4 W User Manual
Congratulations! You have just purchased a Leica E Series stereomicroscope – an excellent choice! Viewing entire objects under magnification imparts perspectives and discoveries that would not be possible with the naked eye. In developing our stereomicroscopes, we have placed great emphasis on simple, self-explanatory operation.
Chapter Overview Safety and Use 6 Leica ES2 14 Leica EZ4 and Leica EZ4 W 22 Eyepieces (only for Leica EZ4) 33 Photography Using the Leica EZ4 W 41 Get Set! 47 The Camera Remote Control (Optional) 55 Care, Transport, Contact Persons 68 Specifications 70 Dimensions 72 Leica E Series User Manual 3
Contents Safety and Use Symbols Used Safety Instructions Regulatory Compliance Information Instructions for Use 7 8 10 12 Leica ES2 Overview: Leica ES2 15 Transport, Placement and Storage 16 The Correct Interpupillary Distance 17 Using the LED Illuminator 18 Focusing 19 Magnification Display 20 Adjusting the Resistance of the Focus Drive 21 Leica EZ4 and Leica EZ4 W Overview: Leica EZ4 23 Instrument Variant: Leica EZ4 W 24 Transport, Placement and Storage 25 The Correct Interpupillary Dist
Contents (Continued) The Camera Remote Control (Optional) Optional Remote Control 56 Viewing Images and Movies With the Optional Remote Control 57 Calling up the Camera Menu 58 COLOR (Automatic White Balance) 59 COLOR (Manual White Balance) 60 EXPOSURE 61 RESOLUTION 62 SETUP CAMERA (Camera Settings) 63 SETUP ETHERNET 64 SETUP WiFi 65 Pairing Cameras With Remotes 67 Care, Transport, Contact Persons Care, Transport, Contact Persons 69 Specifications Specifications 71 Dimensions Leica ES2 Leic
Safety and Use Leica E Series User Manual Safety and Use 6
Symbols Used Read and understand user manual before using this device. Warning of a danger This symbol indicates especially important information that must be read and complied with. Failure to comply can cause the following: • OO Hazards to personnel OO Functional disturbances instruments or Danger due to hot surface This symbol warns against touching hot surfaces, e.g. those of light bulbs.
Safety Instructions Before installing, operating or using the instrument, it is mandatory to read this user manual. In particular, please observe all safety instructions. The "Safety Concept" brochure contains additional safety information regarding service work, requirements and the handling of the microscope, accessories as well as general safety instructions. You can combine individual system articles with articles from external suppliers.
Safety Instructions (Continued) Instrument safety and EMC Our device has been designed, produced and tested in compliance with OO IEC EN 61010-1: Electrical equipment for measurement, control and laboratory use – Safety – Part one: general requirements OO EN 60950-1: Information technology equipment – Safety – Part one: general requirements OO Radio interference suppression in compliance with EN 55011 class B OO Noise immunity in compliance with DIN EN 61326-1.
Regulatory Compliance Information FCC Requirements for Operation in the United States FCC radio frequency interference warnings and instructions This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation.
Regulatory Compliance Information (Continued) Canada (IC) This device complies with Industry Canada licence-exempt RSS standard(s). Operation is subject to the following two conditions: (1) this device may not cause interference, and (2) this device must accept any interference, including interference that may cause undesired operation of the device. Australia and New Zealand Notices This device equipment complies with the Australian and New Zealand regulatory approvals requirements.
Instructions for Use Intended use The E Series microscopes are optical instruments for improving the visibility of objects through magnification and illumination. It is used for observing and documenting. Interference Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
Instructions for Use (Continued) Intervention from the user The microscopes of the E Series contain no components that can be maintained or repaired by the user. Exceptions are mentioned specifically in this manual. Unless otherwise specified in this manual, repair and service work may be carried out by authorized Leica technicians only. Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims.
Leica ES2 Leica E Series User Manual Leica ES2 14
Overview: Leica ES2 1 1. 10× fixed eyepieces for eyeglass wearers 2 2. Carrying handle 3 3. Two-level magnification changer 10× and 30× 4. Focusing drive 4 5 6 7 Leica E Series User Manual 5. Integrated LED incident light illumination 6. Integrated LED transmitted illumination 7.
Transport, Placement and Storage Built-in carrying device All Leica E Series microscopes are equipped with a carrying handle for convenient and safe transport. Leica E Series User Manual Setting up the microscope 1. Place the microscope on a flat tabletop. 2. Connect the power supply to a grounded socket. Packing the device away 1. After use, wind up the cable as shown in the illustration. 2. Store the instrument so that it is protected against dust.
The Correct Interpupillary Distance The ideal image The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. Note for eyeglass wearers If you wear glasses, fold the eyecups back – otherwise, fold the eyecups forwards. Setting the eye distance 1. Look into the eyepieces. 2. Hold the eyepieces with both hands. Push the eyepieces together or separate them until you see a circular image.
Using the LED Illuminator Iluminating the specimen 1. Position a specimen in the middle of the baseplate. Tips on use OO Use incident light for plastic, opaque objects. 2. Switch the two LED illuminators on or off as desired. OO Use transmitted light for transmitted light specimens or transparent objects. OO Use the combination of both LED illuminators for partially transparent objects. After switching off, the LEDs remain lit for about another 4 seconds.
Focusing Focusing raises and lowers the stereomicroscope using the focusing drive. The specimen is brought into sharp focus as soon as it is in the focal point of the objective. Focusing 1. Set the magnification changer to the lowest level (10). 3. Set the magnification changer to the second level (30). 4. Use the focusing drive once again for fine focus. The focusing drive can be operated either leftor right-handed.
Magnification Display You can read the configured magnification on the right-hand rotary knob of the Leica ES2. The additional magnification provided by the eyepieces is already incorporated in the scale.
Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too easy or too difficult to adjust? No problem – the resistance can be adjusted individually depending on your personal preferences: 1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Leica EZ4 and Leica EZ4 W Leica E Series User Manual Leica EZ4 and Leica EZ4 W 22
Overview: Leica EZ4 1 1. 10× fixed eyepieces for eyeglass wearers 2 2. Carrying handle 3 3. Magnification changer 4. Focusing drive 4 5 6 7 Leica E Series User Manual 5. Integrated LED incident light illumination 6. Illumination control 7.
Instrument Variant: Leica EZ4 W 1. 1 2 3 4 5 10× fixed eyepieces for eyeglass wearers 2. Integrated digital camera with SD card slot 3. Mode selection panel 4. Carrying handle 5. Magnification changer 6 6. IR receiver for optional remote control 7 7. Focusing drive 8 9 10 Leica E Series User Manual 8. Integrated LED incident light illumination 9. Illumination control 10.
Transport, Placement and Storage Built-in carrying device All Leica E Series microscopes are equipped with a holder for convenient and safe transport. Leica E Series User Manual Setting up the microscope 1. Place the microscope on a flat tabletop. 2. Connect the power supply to a grounded socket. Packing the device away 1. After use, wind up the cable as shown in the illustration. 2. Store the instrument so that it is protected against dust.
The Correct Interpupillary Distance The ideal image The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. Note for eyeglass wearers If you wear glasses, fold the eyecups back – otherwise, fold the eyecups forwards. Setting the eye distance 1. Look into the eyepieces. 2. Hold the eyepieces with both hands. Push the eyepieces together or separate them until you see a circular image.
Switching on the LED Illuminator Automatic shutoff If the illumination is not operated for 60 minutes, it switches off automatically. The illumination can be reactivated by pressing a button on the membrane keypad. LED illumination 1. Position a specimen in the middle of the baseplate. Leica E Series User Manual 2. Switch the two LED illuminators on or off as desired: OO Use incident light for plastic, opaque objects OO Use transmitted light for transmitted light specimens or transparent objects.
Controlling the Automatic Shutoff Automatic shutoff The factory settings of the EZ4 models switch the instrument off automatically after one hour without user interaction. This automatic shutoff helps to conserve the lightemitting diodes and save energy. Leica E Series User Manual Disabling the automatic shutoff To disable the automatic shutoff, follow these steps: 1. Switch the microscope off. Enabling the automatic shutoff To re-enable the automatic shutoff, follow these steps: 1.
Illumination Types Three-level incident light illumination The incident light illumination is made up of 5 LEDs. Two, three or five diodes are switched on depending on the level. Accordingly, the angle of incidence of the light beam on the object is switched from high to low. Press 1× Five diodes: greatest brightness for incident light objects Leica E Series User Manual Regulating brightness The brightness is adjusted using the keys and affects all switched-on LEDs.
Focusing Focusing raises and lowers the stereomicroscope using the focusing drive. The specimen is brought into sharp focus as soon as it is in the focal point of the objective. Focusing 1. Set the magnification changer to the lowest level (8). 3. Set the magnification changer to the second level (35). 4. Use the focusing drive once again for fine focus. The focusing drive can be operated either leftor right-handed.
Magnification Display You can read the configured magnification on the right-hand rotary knob of the Leica EZ4 models. The additional magnification provided by the eyepieces is already incorporated in the scale Leica EZ4 models with open tubes On the Leica EZ4 model without eyepieces, only the magnification factors of the magnification changer are indicated: 0.8, 1, 1.25, 1.6, 2, 2.5, 3, 3.5. Different total magnifications are possible depending on which eyepieces are used with the Leica EZ4.
Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too easy or too difficult to adjust? No problem – the resistance can be adjusted individually depending on your personal preferences: 1. Grip the drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Eyepieces (Only for Leica EZ4) Leica E Series User Manual Eyepieces (Only for Leica EZ4) 33
Changing Eyepieces (Only for Leica EZ4, Open Version) Changing eyepieces Various eyepieces are available for the Leica EZ4, allowing the stereomicroscope to be adapted even better to the specific requirements.
Eyepieces and Eyecups (Only for Leica EZ4, Open Version) Eyecups The eyecups can be replaced. Risk of infection Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be minimized by the use of individual eyecups.
Dioptric Correction (Only for Leica EZ4, Open Version) Dioptric correction The adjustable eyepieces for the Leica EZ4 are also available with built-in dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises a maximum of ±5 diopter settings. Users with normal vision do not need adjustable eyepieces. An adjustable eyepiece is sufficient for mild shortsightedness. Adjusting 1. Set the dioptric correction for both eyepieces to "0".
Dioptric Correction (Continued) 7. Look into the eyepiece. 8. Slowly rotate the eyepiece individually in the "–" direction until the eye sees the object sharply imaged. 9. Select the highest magnification and refocus if necessary. Now, if you adjust the magnification from the highest to the lowest level, the specimen is always brought into sharp focus. If not, repeat the process.
Graticules (Only for Leica EZ4, Open Version) Graticules The transparent graticules can be inserted in the adjustable eyepieces of the Leica EZ4. They enable easy measurements at the specimen. Available eyepiece inserts Graticules 10 mm/ 0.1 mm 5 mm/ 0.1 mm 5 mm/ 0.05 mm 100 Div / 0.002" For equipment with a graticule in one eyepiece, two adjustable eyepieces are needed.
Graticules: Preparation Care instructions The graticule must always be clean and dust-free! Never touch the graticule with bare hands! Always hold the graticule by the edges! Leica E Series User Manual Variant 1: 10× and 20× eyepieces The 10× and 20× adjustable eyepieces include an insert at the bottom with a snap mount. Variant 2: 16× eyepieces On the 16× adjustable eyepieces, the insert is fixed to the eyepiece by a screwed connection. 1.
Inserting the Graticules Inserting the graticules is the same for all eyepiece types. 4. Put the insert back into the eyepiece. Depending on the variant, you have to either press or twist the insert into place. 6. Align the graticules by turning the eyepiece in the tube. 1. Place the insert with the knurled side on the work surface. 2. Grasp the graticule so that you see the scale in laterally correct form. 3.
Photography Using the Leica EZ4 W Leica E Series User Manual Photography Using the Leica EZ4 W 41
Introduction Thank you for purchasing the Leica EZ4 W by Leica Microsystems. The special design of the Leica EZ4 W makes it a universal, highly versatile tool for viewing microscopic specimens and capturing still images or video.
The Basics Your Leica HD camera has many possibilities for various applications and conditions of use. Below are some basic concepts to help you get the most performance for your application. Modes of use 1. SD mode OO capture onto SD card OO use remote control 2. WiFi mode OO Wireless connection to wireless device (tablet, phone, PC) 5 2 1 4 3 3. USB mode OO Cabled connection to PC 4. Ethernet mode OO Cabled connection to external network 5.
Leica EZ4 W: Overview 1. LED status indicator OO Blinking red means starting up OO Green means ready 2. Capture button for SD card capture 3. Mode selection panel 4.
Leica EZ4 W: Overview (Continued) 1. HDMI for connecting the provided HDMI cable 2. Ethernet socket for connecting an ethernet cable (cable not provided) 3. USB for connecting the provided USB cable 1 2 3 Front Infrared window: Receives the signal from the optional remote control.
Unpacking Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise. The contents include CD with imaging software, HDMI cable, and USB cable. Leica E Series User Manual If you ordered the handheld remote, you received the remote with batteries.
Get Set! Leica E Series User Manual Get Set! 47
USB Mode – Capturing and Adjusting Images Using a Computer 10× eyepieces have a fixed circular field of view of 18 mm or more diameter. The Leica EZ4 W’s field of view, however, is rectangular with a diagonal of 16.4 mm to ensure an even field on the digital image. As a result, the image field of the camera is smaller than the object field seen through the eyepieces. 2. Insert the provided USB cable into the camera’s USB port, and attach the other end of the cable to a USB 2.0 port of your computer. 4.
USB Mode – Capturing and Adjusting Images Using a Computer (Continued) 5. If a mode button other than USB mode is lit green, then press the USB mode button and wait until both the status LED and the USB mode button is green. Note the image aspect ratio while in USB mode is 4:3. To avoid distortion if your projector or monitor is set to a 16:9 aspect ratio, you will need to adjust the aspect ratio of the projector or monitor to 4:3 using the controls of the projector or monitor.
SD Mode – Capturing Images Without a Computer or Without a Wireless Device In order to use this mode, you must have an SD card (not provided) with free memory on it. If the SD memory card is full, the capture button LED will not light green and you will not be able to capture any more images. Attaching the power cable 1. Switch on the power to the EZ4 W microscope. 3.
SD Mode – Capturing Images Without a Computer or Without a Wireless Device (Continued) The capture button LED will turn green. The Leica EZ4 W is now ready to capture images onto the SD memory card. 5. To capture an image onto the SD memory card, lightly press the capture button, located on the side of the Leica EZ4 W camera. The image is now saved on the SD card. Images which are on the SD card can be viewed using the optional remote control. See "Camera Remote Control" on page 55. 6.
Adjusting Settings While in SD Mode Setting a new white balance Make sure that there is no sample in the beam path and that a standard illumination is available when you reset the camera. Return to factory settings Press and hold the service button for 5 seconds. Wait for the beep. The camera will now reset to factory settings. Press and hold the capture button for 5 seconds. Wait for the beep. A new white balance is set.
WiFi Mode – Capturing and Adjusting Images Using a Wireless Device 1. Switch on the power to the Leica EZ4 W microscope. 2. The status light will blink red. Wait until the status light is green and one of the mode button's is also green. This may take approximately 30 seconds. 3. If a mode button other than WiFi mode is lit green, then press the WiFi button and wait until both the status LED and the WiFi mode button is green. 4.
Ethernet Mode – Capturing and Adjusting Images Using a Network 1. Connect one end of an ethernet cable (not provided) to the camera and the other end of the cable to the ethernet connection in your facility. 3. The status light will blink red. Wait until the status light is green and one of the mode button's is also green. This may take approximately 30 seconds. 5. Follow the directions of your network compatible device to connect to your facilities network and select the camera. 6.
The Camera Remote Control (Optional) The remote control is designed to work only when the camera is in SD mode and when the camera is used in combination with an HD display. The only function which works in any mode is the "Info" function which shows details about the camera.
Optional Remote Control The remote control can only work in SD mode except for the "i" button which will display information about the camera on an HD display. The remote must be pointed at the IR receiver of the camera without obstructions. 10 11 12 13 1 3 2 5 6 4 7 8 Leica E Series User Manual 7 9 1. Start/stop video recording 2. Save still image to SD card 3. Stop/continue live image, pause/play movie clip 4. View files on SD card 5. Move to previous image on SD card 6.
Viewing Images and Movies With the Optional Remote Control If you have purchased the optional remote, the Leica HD camera shows images and movie clips directly on an HD monitor. 1. Thumbnail view OO (first press) up/down/left/right select thumbnail OO Show image in full screen or playback movie clip OO next image OO OO / previous image Playback / fullscreen view Play movie clip left /right fast forward / rewind 2. To return to live view press .
Calling up the Camera Menu The remote control can only work when the camera is in SD mode. Opening and closing the camera menu 1. Point the remote control towards the camera. 2. Press the key on the remote control to show the camera menus on the monitor. 3. Press the and buttons to select a menu item. 4. Press the item. button to confirm a menu button again to hide the 5. Press the camera menus.
COLOR (Automatic White Balance) Main menu Enabling automatic white balance 1. Press the button on the remote control. Sub menu color COLOR SET WB PRESS OK EXPOSURE WB MODE AUTO RESOLUTION RED LEVEL SETUP CAMERA BLUE LEVEL SETUP ETHERNET BLACK LEVEL SETUP WIFI HUE 2. Call up the "COLOR" entry. 3. Set the value for "WB MODE" to "AUTO". 4. Press the button to exit the menu.
COLOR (Manual White Balance) Main menu Adjusting the white balance manually (recommended) 1. Move the specimen out of the field of view so that only the illumination is seen. Sub menu color COLOR SET WB PRESS OK EXPOSURE WB MODE MANUAL RESOLUTION RED LEVEL SETUP CAMERA BLUE LEVEL SETUP ETHERNET BLACK LEVEL SETUP WIFI HUE 2. Press the button on the remote control. 3. Call up the "COLOR" entry. 4. Set the "WB MODE" to "MANUAL". SATURATION 5.
EXPOSURE Main menu Automatic exposure 1. Press the button on the remote control. Sub menu exposure COLOR EXP MODE EXPOSURE EXPOSURE RESOLUTION GAIN SETUP CAMERA BRIGHTNESS SETUP ETHERNET GAMMA AUTO 2. Call up the command for automatic exposure (see diagram at the left). 3. Set the value for "EXP MODE" to "AUTO". 4. Correct the values for "BRIGHTNESS" and "GAMMA" until you obtain the desired results for the image.
RESOLUTION LIVE (resolution of the live image) Defines the resolution of the live image. Main menu Sub menu resolution COLOR LIVE HD1080-60 EXPOSURE CAPTURED 5.0MP-JPG RESOLUTION MOVIE HD720-MP4 SETUP CAMERA CAPTURED (resolution of the captured image) Defines the resolution of the captured image on the SD card. MOVIE (resolution of videos) Indicates the resolution of the captured videos on the SD card.
SETUP CAMERA (Camera Settings) FLIP IMAGE Select to flip image 180°. Main menu Sub menu setup camera COLOR RESET CAM PRESS OK EXPOSURE SET DATE/TIME PRESS OK RESOLUTION FLIP IMAGE 0° SETUP CAMERA AUDIO/BEEP ENABLE SETUP ETHERNET LANGUAGE ENGLISH SETUP WIFI SELECT OVERLAY NONE CONFIG OVERLAY LIVE ONLY The camera settings enable you to control internal parameters such as the date or time, reset the camera, etc. RESET CAMERA This command resets the camera to the factory settings.
SETUP ETHERNET Main menu IP ADDRESS You can decide if you want the IP address to be set by your network (Dynamic) or by you using the onscreen keyboard (Static).
SETUP WiFi SECURITY OPTIONS – NONE This means there is no password. Main menu Sub menu WiFi COLOR INTERFACE STANDALONE EXPOSURE NAME (SSID) ICC50 W_2345 RESOLUTION SECURITY OPTIONS NONE SETUP CAMERA PASSWORD (8 CHR) SETUP ETHERNET CHANNEL AUTO SETUP WIFI ENABLE SSID BROADCAST YES This menu allows you to customize parameters relevant to working in WiFi mode. SECURITY OPTIONS – WPA2-PSK AES This allows you to create a password. PASSWORD Create a password using the onscreen keyboard.
SETUP WiFi (Continued) SCAN SSID If "OK" is selected, you will see a list of WiFi access points nearby.
Pairing Cameras With Remotes Pairing The Leica EZ4 W and the remote control can be paired and then only respond to each other. This can be helpful when using multiple cameras and remote controls. 1. Press the process. button to start or end the 3. The microscope only responds to the remote control commands from this remote control. Resetting to factory setting 1. Press the button to start the process. 2. Press the button until a corresponding confirmation is displayed on the HD monitor.
Care, Transport, Contact Persons Leica E Series User Manual Care, Transport, Contact Persons 68
Care, Transport, Contact Persons General Instructions OO Keeping all optical components clean is important for maintaining good optical performance. OO Avoid excessive use of solvents. The lintfree cloth, lens cloth or cotton swab should be soaked with solvent, but not so wet that solvent runs over the lens. Always protect the microscope and camera with the plastic cover when the instrument is not in use. (Included in the delivery package of the microscope.
Specifications Due to the technology of the camera, the specifications are changing regularly. Please visit our website www.leica-microsystems.com/ education for the latest brochure downloads to find the up to date specifications.
Specifications Operating environment Feature Specification Temperature Operating Storage +10°C to +40°C ( 50°F – 104°F) -20°C to +55°C ( -4°F – 131°F) Relative humidity (maximum) Operating/Storage 10 % to 90 % (noncondensing) Altitude (maximum) Operating Nonoperating 0 m to 2,000 m (0 ft to 6,561 ft) 0 m to 10,668 m (0 ft to 35,000 ft) Pollution Degree Rating 2 Weight 4.6 kg Ports and connectors Feature Specification EZ4 W: USB one standard USB 2.
Dimensions Leica E Series User Manual Dimensions 72
Leica ES2 Leica E Series User Manual Dimensions 73
Leica ES2 (Continued) Leica E Series User Manual Dimensions 74
Leica EZ4 W Leica E Series User Manual Dimensions 75
Leica EZ4 W (Continued) Leica E Series User Manual Dimensions 76
Leica EZ4, 10× Eyepieces Leica E Series User Manual Dimensions 77
Leica EZ4, 10× Eyepieces (Continued) Leica E Series User Manual Dimensions 78
Leica EZ4 without Eyepieces Leica E Series User Manual Dimensions 79
Leica EZ4, without Eyepieces (Continued) Leica E Series User Manual Dimensions 80
The statement by Ernst Leitz in 1907, “With the User, For the User,” describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life.
