Leica DM750 P Manual
Chapter overview Safety regulations 4 The Leica DM750 P 15 Get Ready! 18 Get Set! 24 Go! 36 Care of the Microscope 38 Dimensions 42 Leica DM750 P Manual 2
Contents Safety regulations Safety concept Symbols used in this operating manual Important Notes Instructions on use Health risks and dangers of use Information for the person responsible for the instrument Care instructions Accessories, maintenance and repair Electrical data and ambient conditions 4 5 6 7 8 10 11 12 13 14 The Leica DM750 P 15 Introduction 16 Unpacking 17 Get Ready! Attaching Analyzer Module or Analyzer/ Bertrand Lens Module Viewing Tube Installing Objectives Substage Cond
Safety regulations Leica DM750 P Manual 4
Safety concept The individual modules of the Leica DM microscopy series include an interactive CD-ROM with all relevant user manuals in several languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.leica-microsystems.com.
Symbols used in this operating manual Warning of a danger This symbol indicates especially important information that must be read and complied with. • Failure to comply can cause the following: ϘϘ Hazards to personnel ϘϘ Functional disturbances or damaged instruments Warning of hazardous electrical voltage This symbol indicates especially important information that is mandatory to read and observe. Danger due to hot surface. This symbol warns against touching accessible hot surfaces, e.g.
Important Notes Description The Leica DM750 P microscope meets today's state of the art of technology. Nevertheless, hazards may still arise during operation. The potential risks are described below. User Manual This user manual includes important instructions related to operating safety, maintenance and accessories, with an interactive CD-ROM with all Accessories from third-party suppliers You can combine individual system articles with articles from external suppliers.
Instructions on use Intended use The Leica DM750 P microscope is an optical instrument which uses magnification and illumination to improve the visibility of objects. It is used for observing and documenting. The Leica DM750 P microscope may be used only in closed rooms and must be placed on a solid floor. The Leica DM750 P microscope can be used in clean rooms without any problems. • Position the Leica DM750 P microscope so that it can be disconnected from the power supply at any time.
Instructions on use (continued) • consulted whenever the instrument is The responsible Leica affiliate must be altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual. • or noncompliant use shall void all rights Unauthorized alterations to the device to any warranty claims. Transport If at all possible, use the original packaging for shipping or transporting individual modules.
Health risks and dangers of use Health risks Workplaces with microscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal problems may occur. For this reason, appropriate measures for reduction of the workload must be taken: The ergonomic design and construction of the Leica microscopy series are intended to reduce the exertion of the user to a minimum.
Information for the person responsible for the instrument Information for the person responsible for the instrument ϘϘ Ensure that the Leica DM750 P microscope is used only by qualified personnel. ϘϘ Ensure that this user manual is always available at the Leica DM750 P microscope. ϘϘ Carry out regular inspections to make certain that the authorized users are adhering to safety requirements. ϘϘ ϘϘ ϘϘ Do not use the Leica DM750 P microscope unless it is in perfect condition.
Care instructions General instructions ϘϘ Protect the Leica DM750 P microscope against damp, vapors, acids, alkalis, and corrosive substances. Do not store chemicals in the vicinity. ϘϘ Protect the Leica DM750 P microscope from oil and grease. Never grease or oil mechanical parts or sliding surfaces. Cleaning coated parts and plastic parts ϘϘ Dust and dirt particles should be removed with a soft brush or lint-free cotton cloth. ϘϘ ϘϘ Remove coarse debris with a moistened disposable cloth.
Accessories, maintenance and repair Accessories Only the following accessories may be used with the Leica DM750 P microscope: ϘϘ The Leica accessories described in this user manual. ϘϘ Other accessories, provided that these have been expressly approved by Leica as being technically safe in this context. Leica DM750 P Maintenance ϘϘ The Leica DM750 P microscope is basically maintenance-free.
Electrical data and ambient conditions Fuse Replacement Unplug the instrument before changing any fuses. The Leica DM750 P has two fuses, which are located behind the power cord receptacle.
The Leica DM750 P Leica DM750 P Manual 15
Introduction Thank you for purchasing the Leica DM750 P Polarizing Microscope System from Leica Microsystems. The Leica DM750 P is a highly adaptable instrument with superior optics and precision engineering for educational and basic industrial polarized light microscopy applications.
Unpacking ϘϘ Carefully remove the microscope and any components from the packing carton. ϘϘ Check the components against the planned configuration. ϘϘ Optional items such as contrast accessories, camera adapters, cameras, and carrying cases are not shipped as part of the standard equipment. These items are delivered in separate packages. ϘϘ Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise.
Get Ready! Leica DM750 P Manual 18
Attaching Analyzer Module or Analyzer/Bertrand Lens Module Tools used ϘϘ Allen key 2. Align the shape of the module with the shape of the microscope stand. 4. Tighten the set screw to lock the module into place. 1. Slightly unscrew the set screw located on top of the stand with the Allen key tool provided. 3. Insert the dovetail of the module into the stand. (Analyzer/Bertrand Lens Module shown).
Viewing Tube 1. Slightly unscrew the set screw located on the top of the Analyzer module. 5. If you wear eyeglasses for microscope viewing, keep the rubber eyeguards folded down. If you do not wear eyeglasses, you may find it useful to unfold the rubber eyeguards in order to block out ambient room light. reticle into the right eyetube so that Install the eyepiece with the crossline 2. Align the shape of the polarizing viewing tube with the shape of the module. 3.
Installing Objectives scope configuration, you will notice the If you have purchased a standard micro- objectives are already installed on the nosepiece and the substage condenser is already installed on the stand. In this case, go to section "Operation". If you purchased your Leica DM750 P by components and not by standard configuration, please continue in this section. 1.
Substage Condenser Substage Condenser The Leica DM750 P has an open substage condenser mount. Therefore, the condenser needs to be installed. 2. Adjust the condenser mount to its lowest position using the condenser focus knob on the left-hand side of the substage. 3. Swing the polarizer, which is connected to the bottom of the condenser mount, to the left. 1. Raise the stage to its highest position using the coarse focus knob on the right side of the microscope.
Substage Condenser (cont.) 4. Completely loosen the two thumbscrews on the condenser mount. 6. Raise the condenser to its highest position using the condenser focus on the left-hand side of the substage 7. Tighten the thumbscrews to roughly center the substage condenser so that the top lens of the condenser is centered beneath the objective in use. You will more accurately center and focus the condenser when you reach section Koehler Setup. 5.
Get Set! Leica DM750 P Manual 24
Basic setup has been grounded to the microscope As a safety precaution, the power cord base. Never use an adapter between the power cord and the power source; it will render the grounding feature ineffective. Work Surface Always use your microscope on a hard, stable surface. • 2. Plug the microscope power cord into a suitable grounded electrical outlet. A grounded 3-wire cord is provided. 4. Turn on the microscope using the switch on the bottom right-hand side of the microscope stand. 3.
Using the Aperture Condenser diaphragm, which can be adjusted to The condenser is furnished with an iris match the effective numerical aperture of the objective. 1. To open and close this diaphragm, simply rotate the knurled condenser ring to the right or to the left so that the line on the rotating ring aligns with the objective magnification in use. 2. To start, fully open the iris diaphragm of the aperture condenser by rotating the ring to the extreme right.
Setting up Polarized Light substage condenser in a swing in/out The polarizer is located under the mount. Swing it into place and be sure it "clicks" into place. Leica DM750 P 1. Loosen the polarizer locking thumbscrew, which is located on the right-hand side of the polarizer. Manual 2. Rotate the polarizer to the zero position.
Setting up Polarized Light (cont.) between the viewing tube and the stand The Analyzer is located in the module either in a slider module or in an Analyzer Bertrand Lens module. If you have a slider, slide the Analyzer into position. Leica DM750 P 3.
Focusing Focusing 1. Place a polarized light specimen on the stage. 3. Raise the microscope stage using the coarse adjustment knob until you reach its positive stop or the specimen touches the objective. 2. Rotate the 10× objective into working position. Leica DM750 P 4. Look into the eyepieces and adjust the illumination intensity so that it is comfortable for viewing. 5. Using the fine focus adjustment knob, bring the specimen into sharp focus.
Viewing Tube Adjustment Adjust the eyetubes for your interpupillary distance. 1. Fold or unfold the eyetubes until you see one illuminated circle. 2. Focus the crosshair reticle by gripping the knurl and rotating the top of the eyepiece until the reticle is in clear focus. 3. While looking through only the crosshair eyepiece, bring the specimen into focus with the fine focus of the microscope. tube length for all interpupillary settings.
Koehler Set up Koehler Set up 1. Close the Koehler field diaphragm on the base of the microscope so that its iris leaves are present within the field of view when looking into the eyepieces. 3. Turn the condenser centering thumbscrews simultaneously to center the image of the field diaphragm in the field of view. 2. Use the condenser focus knob on the left-hand side of the substage to bring the leaves of the field diaphragm into sharp focus. 4.
Objective Centration Tools used ϘϘ Centering keys 2. Focus the polarizing sample. 3. Move a noticeable point of the sample to the center of the crosslines. 1. Retrieve the two centering keys from the packaging and insert the centering tools into the centering holes located above the objective in use. Engage the ball end of the centering tools into the set screws, which are hidden in the centering holes. 4.
Objective Centration (cont.) 5. Adjust the objective centering screws until the noticeable point of the sample is midway between the furthest position and the center of the crosslines. This moves the objective center closer to the stage center. 6. Move the specimen (not the objective centering screws!) so that the noticeable point is at the center of the crosslines once again. 7. Check to make sure that the noticeable point stays at the center of the crosslines when the stage is rotated.
Bertrand Lens Operation module, you can view a conoscopic If you have an Analyzer/Bertrand Lens image through the eyepieces. 1. Bring the portion of the specimen you would like to view in conoscopy (with the Bertrand Lens) to the center of the field through the eyepieces. You can now view the conoscopic image 3. If necessary, you can fine tune the Bertrand Lens centration with the tools provided. 4.
Time Delay Shutoff a time delay shutoff capability, which The Leica DM 750 P is equipped with Changing the status of the time delay shutoff automatically turns the illumination off after 2 hours of no changes in the intensity control. ϘϘ The time delay shutoff IS enabled as a default. ϘϘ The LED Illumination will flash to indicate the time delay shutoff status was changed. ϘϘ The LED Illumination will flash two times slowly then stay on when you disable the Time Delay Shutoff.
Go! Leica DM750 P Manual 36
Ready! Set! Go! Now all you need to do is change objectives, set the aperture condenser and field diaphragm appropriately for the objective magnification in use, and enjoy the view! Leica DM750 P Manual 37
Care of the Microscope Leica DM750 P Manual 38
General maintenance General Always carry the microscope using two hands. There is a handle on the back of the microscope and an undercut in the front for this purpose. • Leica DM750 P ϘϘ The cord wrap allows you to wrap the cord in such a way that only the length you need is extended. ϘϘ Proper storage of accessories in the appropriate positions is important to keep them safe from loss or damage.
General maintenance (cont.) ϘϘ Keeping all optical components clean is important for maintaining good optical performance. ϘϘ The microscope should always be covered with the plastic dust cover (provided with the instrument) when it is not in use. ϘϘ If any optical surface becomes coated with dust or dirt, clean the surface by blowing it off with a syringe or brushing it off with a camel hair brush before attempting to wipe the surface clean.
Mechanical Maintenance Mechanical Maintenance ϘϘ Use the dust cover. It is the most important way to keep the microscope in good mechanical and physical condition. ϘϘ The Leica DM750 P has a durable finish. All non-optical parts of the instrument can be cleaned with methanol, naptha, or soap and water. Avoid the use of all other organic solvents when cleaning the instrument.
Dimensions Leica DM750 P Manual 42
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