Leica DM500, DM500 B Manual
Chapter overview Safety regulations 4 The Leica DM500 , DM500 B 15 Get Ready! 18 Get Set! 25 Go! 34 Care of the Microscope 36 Dimensions 39 Leica DM 500, DM500 B Manual 2
Contents Safety regulations Safety concept Symbols used in this operating manual Important Notes Instructions on use Health risks and dangers of use Information for the person responsible for the instrument Care instructions Accessories, maintenance and repair Electrical data and ambient conditions 4 5 6 7 8 10 11 12 13 14 The Leica DM500, DM500 B 15 Congratulations! 16 Unpacking 17 Leica DM 500, DM500 B Get Ready! 18 Attaching the viewing Tubes 19 Leica EZ Viewing Tube – integrated eyepiec
Safety regulations Leica DM 500, DM500 B Manual 4
Safety concept The individual modules of the Leica DM microscopy series include an interactive CD-ROM with all relevant user manuals several languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.leica-microsystems.com.
Symbols used in this operating manual Warning of a danger This symbol indicates especially important information that must be read and complied with. • Failure to comply can cause the following: ϘϘ Hazards to personnel Danger due to hot surface. This symbol warns against touching accessible hot surfaces, e.g. those of light bulbs.
Important Notes Description The Leica DM500 and DM500 B microscope meets today's state of the art of technology. Nevertheless, hazards may still arise during operation. The potential risks are described below. • Before installing, operating or using the instruments, it is required to read this user manual. In particular, please observe all safety instructions.
Instructions on use Intended use The Leica DM500 and DM500 B microscopes are optical instrument which use magnification and illumination to improve the visibility of objects. It is used for observing and documenting. The Leica DM500 and DM500 B microscopes may be used only in closed rooms and must be placed on a solid floor. The Leica DM500 or DM500 B microscopes can be used in clean rooms without any problems.
Instructions on use (continued) • consulted whenever the instrument is The responsible Leica affiliate must be altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual. • or noncompliant use shall void all rights Unauthorized alterations to the device to any warranty claims. Transport If at all possible, use the original packaging for shipping or transporting individual modules.
Health risks and dangers of use Health risks Workplaces with microscopes facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal problems may occur.
Information for the person responsible for the instrument Information for the person responsible for the instrument ϘϘ Ensure that the Leica DM500 or DM500 B microscope is used only by qualified personnel. ϘϘ ϘϘ Do not use the Leica DM500 or DM500 B microscope unless it is in perfect condition. ϘϘ Inform your Leica representative or Leica Microsystems (Schweiz) AG, Industry Division, 9435 Heerbrugg, Switzerland, immediately of any product defect that could potentially cause injury or harm.
Care instructions General instructions ϘϘ Protect the Leica DM500 or DM500 B microscope against damp, vapors, acids, alkalis, and corrosive substances. Do not store chemicals in the vicinity. ϘϘ Protect the Leica DM500 or DM500 B microscope from oil and grease. Never grease or oil mechanical parts or sliding surfaces. Cleaning coated parts and plastic parts ϘϘ Dust and dirt particles should be removed with a soft brush or lint-free cotton cloth.
Accessories, maintenance and repair Accessories Only the following accessories may be used with the Leica DM500 or DM500 B microscope: ϘϘ The Leica accessories described in this user manual. ϘϘ Other accessories, provided that these have been expressly approved by Leica as being technically safe in this context. Leica DM 500, DM500 B Maintenance ϘϘ The Leica DM500 and DM500 B microscopes are basically maintenance-free.
Electrical data and ambient conditions Fuse Replacement Unplug the instrument before changing any fuses. The Leica DM500 has two fuses, which are located behind the power cord receptacle. • Use only fuse type: 5×20 mm, 1 Amp/250V, Quick acting (# 13RFAG30003) Electrical data DM500 DM500 B • Input: 100-240 VAC, 50/60 Hz, 5 W (3 W LED) Input: 100-240 VAC, 50/60 Hz, 0.5-0.2 A OR 4× 1.2 V AA rechargeable batteries Use only 1.2 Volt NiMH type AA rechargeable batteries with a maximum of 2700 mAh.
The Leica DM500, DM500 B Leica DM 500, DM500 B Manual 15
Congratulations! Thank you for purchasing the Leica DM500 or DM500 B Compound Microscope from Leica Microsystems. This model’s exclusive design features and full range of accessories make it a truly versatile, high-quality instrument for educational and routine clinical applications.
Unpacking ϘϘ Carefully remove the microscope and any components from the packing carton. ϘϘ Check the components against the planned configuration. ϘϘ Optional items such as contrast accessories, camera adapters, cameras, and carrying cases are not shipped as part of the standard equipment. These items are delivered in separate packages. ϘϘ Please do not discard any of the packing materials. They should be used for safely storing and transporting the instrument should the need arise.
Get Ready! Leica DM 500, DM500 B Manual 18
Attaching the viewing Tubes Tools used ϘϘ Allen key There are two types of viewing tube. Identify which viewing tube you have: Type 1: Leica EZ viewing tube with integrated eyepieces. 1. Slightly unscrew the set screw (located on top of the stand) with the Allen key tool provided. 2. Insert the dovetail into the stand support and tighten the set screw firmly without forcing it. This precisely positions the viewing tube onto the optical axis of the microscope, regardless of the selected rotation.
Leica EZ Viewing Tube – integrated eyepieces 1. To use the captive thumbscrew, completely remove the set screw that comes with the stand. 2. Position the Leica EZ viewing tube back in place on the stand. Leica EZ viewing tube with integrated eyepieces. To rotate the Leica EZ viewing tube, either loosen the set screw on the stand or replace the set screw with the (optional) captive thumbscrew.
Leica EZ Viewing Tube – integrated eyepieces (cont.) You can now safely rotate the Leica EZ viewing tube by loosening the captive thumbscrew, rotating the viewing tube, and tightening the captive thumbscrew again. The eyepieces are already integrated with the Leica EZ viewing tube and are pre-adjusted for corrected vision; therefore no eyepiece adjustment or insertion is necessary. Go on to the section "Eyeguards" on page 23.
Leica Standard Viewing Tube – separated eyepieces 1. Insert the eyepieces into the eyetubes. 2. Lock the eyepieces into the eyetubes by tightening the silver screws on the underside of the eyetubes with a standard Cross type (Phillips) screwdriver (not provided). Standard viewing tube; eyetubes do not yet contain the eyepieces The standard viewing tube has a rotatable dovetail. Therefore, you can now rotate the standard viewing tube freely in any orientation.
Eyeguards If you wear eyeglasses for microscope viewing, keep the rubber eyeguards folded down. If you do not wear eyeglasses, you may find it useful to unfold the rubber eyeguards in order to help block out ambient room light. Leica DM 500, DM500 B If you have purchased a standard microscope configuration, you will notice that the objectives are already installed on the nosepiece, and the substage condenser is already installed on the stand. In this case, go to section "Operation" on page 26.
Installing Objectives and Substage Condenser Installing Objectives Always rotate the nosepiece by using the knurled nosepiece ring. • 1. As you rotate the nosepiece clockwise, attach the objectives by screwing them into the nosepiece holes starting with the lowest magnification and advancing to the highest magnification. Leica DM 500, DM500 B Substage Condenser The Leica DM500’s and DM500 B's substage condenser is pre-installed, prefocused, and pre-centered.
Get Set! Leica DM 500, DM500 B Manual 25
Turning on the microscope As a safety precaution, the power cord has been grounded to the microscope base. Never use an adapter between the power cord and the power source; it will render the grounding feature ineffective. Work Surface Always use your microscope on a hard, stable surface. • Power Cord If the power cord is not already attached, attach it securely to the back of the microscop. • If you have a DM500 B with a rechargeable base: 1. Insert the batteries in the underside of the base 2.
Using the Condenser Plug in and Turn on the Microscope 1. Plug the microscope power cord into a suitable grounded electrical outlet. Using the Condenser The condenser is furnished with an iris diaphragm, which can be adjusted to match the effective numerical aperture of each objective. 2. To start, fully open the iris diaphragm of the aperture condenser by rotating the condenser ring to the extreme right. A grounded 3-wire cord is provided. 2.
Prepare to View a Specimen Slide Prepare to View a Specimen Slide 1. Place a specimen slide on the stage by sliding the specimen slide forward into the slide grips. 2. Use the X/Y stage control to position the slide so a portion of the specimen is under the objective in use. Slide grips hold the slide in place.
Focusing Focusing 1. Rotate the nosepiece (using the knurled nosepiece ring) to move the lowest magnification objective into working position. 2. Raise the stage by rotating the coarse adjustment knob to its positive stop at the highest position. 3. Look into the eyepieces and adjust the illumination intensity so that it is comfortable for viewing. The Leica DM500 or DM500 B microscope stand has been factory-adjusted so that the stage focus can be found within 1.
Viewing Tube Adjustment 1. Adjust the eyetubes for your interpupillary distance. Fold or unfold the eyetubes to decrease or increase the distance between the eyepieces until you see one illuminated circle. Leica EZ Viewing Tube If you are using a Leica EZ viewing tube, which already has the eyepieces integrated with the eyetubes, there are no additional adjustments necessary. Be sure you are wearing your eyeglasses or contact lenses. Go on to section "Oil Immersion" on page 32.
Viewing Tube Adjustment (cont.) If you are comfortable wearing your corrective lenses (contact lenses or eyeglasses) for microscope viewing, leave them on and your adjustments will be minimal. 2. Focus on the specimen using the fine adjustment knob of the microscope while looking only through one of the eyepieces (if you have one focusable and one nonfocusable eyepiece, look through the nonfocusable eyepiece at this time). To help focus, cover or close the other eye. 3.
Oil Immersion Technique Oil Immersion Technique 1. Find the field of view on the slide that you would like to inspect. 3. Place a drop of Leica Immersion Oil on the specimen slide on the area of the specimen that you were inspecting. 5. Raise the stage slowly using the coarse focus knob until the drop of oil on the specimen slide just comes in contact with the front of the oil immersion objective lens. 2. Lower the stage to its lowest position using the coarse focus knob. 4.
Oil Immersion Technique (cont.) 7. Look through the microscope and slowly rotate the fine focus knob to raise the stage until the specimen is in focus. 8. When finished using the oil immersion objective, be sure to clean the front of the objective, the specimen slide, and any other surfaces that the oil contacted using the instructions in section "Care of the Microscope" on page 37.
Go! Leica DM 500, DM500 B Manual 34
Ready! Set! Go! Now all you need to do is change objectives, set the condenser appropriately for the objective magnification in use, and enjoy the view! Leica DM 500, DM500 B Manual 35
Care of the Microscope Leica DM 500, DM500 B Manual 36
General maintenance General Always carry the microscope using two hands. There is a handle on the back of the microscope and an undercut in the front for this purpose. ϘϘ • Leica DM 500, DM500 B The cord wrap allows you to wrap the cord in such a way that only the length you need is extended. ϘϘ Keep all optical components clean. Cleanliness is important for maintaining good optical performance.
General maintenance (cont.) ϘϘ No part of the microscope is quite so vulnerable to collecting dirt, dust, and oil as the front lens of the objective. Whenever you encounter lack of contrast, cloudiness or poor definition, carefully check the condition of the front lens with a magnifier. ϘϘ The lower magnification objectives have fairly large front lenses and can be cleaned with a cloth or lens tissue wrapped around a finger and moistened with methanol.
Dimensions Leica DM 500, DM500 B Manual 39
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