Leica M165 FC Leica M205 FA User Manual
General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains additional information about handling and care. Use in clean rooms The Leica M series can be used in clean rooms without any problems. Cleaning ρρ Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning. ρρ Never use chemicals to clean colored surfaces or accessories with rubberized parts.
Important Safety Notes User Manual The individual modules of the Leica M stereomicroscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.stereomicroscopy.com. You can combine individual system articles with articles from external suppliers (e.g. cold light sources, etc.).
Symbols Used Warning of a danger This symbol indicates especially important information that must be read and complied with. • Failure to comply can cause the following: ρρ Hazards to personnel ρρ Functional disturbances or damaged instruments Danger due to hot surface This symbol warns against touching accessible hot surfaces, e.g. those of light bulbs. Important information This symbol indicates additional information or explanations that intend to provide clarity.
Safety Instructions Description The individual modules fulfill the highest requirements for observation and documentation of Leica stereomicroscopes of the M series. Intended use ▶▶ Refer to "Safety Concept" booklet Non-intended use ▶▶ Refer to "Safety Concept" booklet Never use M series microscopes or their components for surgical procedures (e.g. on the eye) unless they are specifically intended for that purpose.
Safety Instructions (cont'd.) Repairs, service work ▶▶ Refer to "Safety Concept" booklet ρρ Only original Leica Microsystems spare parts may be used. ρρ Before opening the instruments, switch off the power and unplug the power cable. Touching the live circuit can cause injury. Transport ρρ Use the original packaging for shipping or transporting the individual modules of the Leica M stereomicroscopy series and the accessory components.
Safety Instructions (cont'd.) • tial transmission method for bacterial Direct contact with eyepieces is a poten- and viral infections of the eye. The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups. Leica M-series Light sources: Safety regulations Protective measures of the manufacturer: ρρ UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays.
Safety Instructions (cont'd.) Supply unit Always unplug the supply unit from the power supply: ρρ When installing and disassembling the lamp housing ρρ Before opening the lamp housing ρρ When replacing the high-pressure mercury lamp and other parts, such as the heatabsorbing filter or the collector Lamp housing Never open the lamp housing while the lamp is switched on. Risk of explosion, UV exposure, blinding! • • it to cool off for at least 15 minutes.
Contents General Instructions Important Safety Notes Symbols Used Safety Instructions 2 3 4 5 The Leica M Series for Fluorescence Congratulations! A Step Towards Infinity The Electronics: Comfort, Convenience and Safety The Modular Design: Everything is Relative Maximum Compatibility On We Go 13 14 15 16 17 18 Assembly Assembling the Focusing Column for MDG Bases Assembling the Focusing Column with an Incident-light Base Motorized Focus: Restricting the Travel Path Optics Carrier Tube Ey
Quick Start Guide The Fastest Route to Success Overview of an M Series Microscope The Correct Interpupillary Distance Using the Eyepieces Focusing Adjusting the Resistance of the Focus Drive Changing Magnification (Zoom) Ratchet Steps and Magnification Levels Parfocality: More Comfort and Convenience for Your Work Iris Diaphragm 64 65 66 67 68 69 70 71 72 73 Eyepieces Magnification Factors of the Eyepieces Health Notes Dioptric Correction Dioptric Correction and Parfocality Graticules 75 7
System Illumination Leica LED5000 MCI™ Leica LED5000 RL Leica EL6000 – About the Instrument Leica EL 6000 – Operation Leica EL 6000 – Troubleshooting Cleaning and Maintenance 106 108 109 111 112 113 Dimensional Drawings Leica M165 FC Leica M205 FA Leica LED5000 MCI Leica LED5000 RL MATS TL 129 132 135 136 137 Specifications Leica M165 FC / Leica M205 FA Leica EL6000 – Specifications Leica TL ST Transmitted-light Base Leica TL BFDF Transmitted-light Base Leica TL RC™ / TL RCI™ Leica IsoPr
The Leica M Series for Fluorescence Leica M-series User Manual 12
Congratulations! Congratulations on obtaining your new Leica M series stereomicroscope. We are convinced it will exceed your expectations, as never before have we applied our decades of experience in the areas of optics, mechanical engineering and ergonomics in such an uncompromising manner. The Leica M series embodies all the qualities you associate with the name Leica Microsystems: excellent objectives, high-quality engineering, and reliability.
A Step Towards Infinity Ever since their introduction by Horatio S. Greenough, stereomicroscopes have worked according to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engineers have worked to push magnification, resolution and image fidelity to the limit permitted by optics.
The Electronics: Comfort, Convenience and Safety for Your Experiments Never before have electronics been used as extensively in a Leica series as in the new M series lineup. Optics carrier, stand, base and illuminator are all connected using electrical contacts—which provides a number of advantages. Reliability for your experiments Exact reproducibility of results is an increasingly common demand, particularly in research and development.
The Modular Design: Everything is Relative The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades. The optics carriers, eyepieces, bases and more can be combined in any way you choose, allowing you to create the microscope that best suits your needs. Leica M-series Despite this, you will notice that the controls and individual components do not differ significantly.
Maximum Compatibility Leica engineers were careful to ensure that the new Leica M series—like its predecessors— remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All new objectives of the Leica M series are parfocal, meaning that when used with the objective nosepiece, they can be replaced while keeping the specimen in perfect focus.
On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on Page 63. Leica M-series If, on the other hand, you are assembling the microscope yourself, continue with the "Assembly" chapter, which begins on Page 19. User Manual For everything you need to know about the correct use of fluorescence-related parts, refer to Page 114.
Assembly Leica M-series User Manual 19
Assembling the Focusing Column for MDG Bases The first step is to connect the focusing column of the M series to the corresponding base. Assembling the column adapter 1. Securely install the column adapter on the column using the four included screws. Assembling the focusing column 2. Securely screw the focusing column to the base using the six included screws.
Assembling the Focusing Column with an Incident-light Base When using an incident-light base, the focusing column and motorized focus are installed directly on the base; no extension plate is required. Assembly 1. Place the focusing column on the side. 3. Screw the base securely onto the focusing drive. Tools used ρρ Hex socket screwdriver, 3 mm 2. Insert the four screws provided into the outer holes of the base.
Motorized Focus: Restricting the Travel Path Depending on the work situation, it is useful to restrict the maximum travel path of the stereomicroscope. This prevents injury during handling of the sample caused by fingers or hands becoming trapped or the specimen touching the objective or even being damaged by it. Restricting the bottom travel range 1. Move the motorized focus into the lowest position you want to reach. 2. Unscrew the screw of the limit stop on the side of the focusing column. 3.
Optics Carrier Tools used ρρ Hex socket screwdriver, 4 mm Leica M-series Assembling the optics carrier 1. Place the optics carrier on the focusing column so that the screw fits into the thread provided and the lug fits into the groove. User Manual 2. Press the optics carrier backwards to the focusing column and screw it in place using your other hand.
Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used ρρ No tools required. Assembling the tube 2. Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove. 3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position. Preparations 1.
Eyepieces Tools used ρρ No tools required. Magnification range You can extend the overall magnification range using available 10×, 16×, 25× and 40× widefield eyepieces for persons wearing glasses. Preparation 1. If you want to use an optional graticule, insert it now (Page 79). Inserting the eyepieces 3. Push the eyepieces into the tubes as far as they will go and check to ensure that they fit tightly and accurately. 2. Remove the plastic tube guard. 4. Securely tighten the clamping screws.
Objective Tools used ρρ No tools required. Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective Hold the objective firmly during assembly and disassembly so that it does not fall onto the stage plate. This applies particularly to the 2x planapochromatic objective, which is very heavy. Remove all specimens from the stage plate first. • Alternative fastening options ρρ If using the objective nosepiece, read the instructions on Page 28.
UV Protection Screen: Assembly Tools used ρρ Allen key Use with one objective 1. Adjust the UV protection screen laterally using the arm. Intended use The UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays. 2. Unscrew the hexagon-head screw. Safety Notes UV radiation can damage the eyes. Therefore, it is mandatory to install the UV filter and adjust it correctly. 4. Tighten the hexagon-head screw. • 3.
Objective Nosepiece – Assembly Preparations Hold the objectives firmly during assembly and disassembly so that they do not fall onto the stage plate. • 2. Rotate the moving part by 90° and attach the objective nosepiece to the drive housing from the front (!). Screw the objective nosepiece firmly into place. ρρ Move the drive housing all the way upwards and remove the optics carrier, if the carrier has already been installed. Assembly 1. Remove the transport anchor from the objective nosepiece. 4.
Objective Nosepiece – Adjusting Parfocality The following procedure only has to be carried out once. Afterwards, both objectives are parfocal, meaning that the specimen remains in focus when the objective nosepiece is rotated. Adjustment 1. Rotate the 2× objective into the beam path and set it to the lowest magnification. 2. Focus on the specimen. This procedure must be repeated if you replace either of the two objectives with another.
Transmitted-light Base TL ST Unpacking the base The base is delivered with the adapter plate installed. Make sure the instruments are unpacked on a flat, sufficiently dimensioned, and non-slip surface. Leica M-series Focusing drive and column 1. Unscrew the extension plate from the base using the Allen key provided. 2. Attach your focusing drive column from below using the four hexagon-head screws. 3. Re-attach the adapter plate to its original position using the six hexagon-head screws.
FluoCombi III™ – Preparations Before assembling the FluoCombi™, you have to disconnect all instruments from the power supply. Failure to observe these instructions can result in damage to the microscope and the connected instruments. 2. Screw the two screws into the holes provided for storage. 4. Screw the other planapochromatic stereo objective into the FluoCombi III™. Preparations 1. Remove the two screws used as transport anchors.
FluoCombi III™ – Assembly 1. Guide the FluoCombi III™ in from below, while holding it flat. Ensure that it is aligned with the holder on the focusing column at the left and right. Leica M-series 2. Hold the FluoCombi III™ securely and screw it to the focusing column using the provided screw.
FluoCombi III™ – Assembly (cont'd.) Assembling the optics carrier 1. Unscrew the three screws and the adapter ring on the optics carrier. 2. Attach the optics carrier to the FluoCombi III and screw it into place. 4. Assemble the tube (see Page 24). 5. Assemble the illuminator (see Page 51 ff.) 3. Open the iris diaphragm completely.
FluoCombi III™ – Adjusting the Parfocality Setting up the objective nosepiece The following configuration only has to be carried out once. It ensures that the parfocality remains intact, and thus the specimen does not need to be brought into focus again. 1. Place a specimen under the microscope. 7. Unscrew the clamping screw on the objective. 2. Rotate the stereo planachromatic objective under the beam path. 3. Reduce the view so that you can see the specimen in the overview. 4.
FluoCombi III™ – Adjusting the Parfocality (cont'd.) 9. By turning the objective ring, focus on the specimen until the specimen appears absolutely sharp. 11. Refocus. 12. Tighten the clamping screw (see Step 7). 13. Rotate the stereo planachromatic objective under the beam path. This causes the two objectives to be parfocal. This means that from then on, you can toggle between the two objectives without refocusing. 10.
FluoCombi III™ – Centering the Objectives The following configuration only has to be carried out once. It ensures that the specimen remains in the optical center when the objective is changed. Centering the objective 1. Rotate the 5× planachromat under the beam path. 2. Focus on the midpoint of the specimen. To attain optimum results for the following settings, insert a graticule into the eyepiece. This makes locating the center vastly easier. Leica M-series 4.
FluoCombi III™ – Filter and UV Protection Screen Inserting the dichroic mirror 1. Pull the dummy filter carrier out of the holder. Assembling the UV protection screen 1. Screw the UV protection screen to the holder provided on the optics carrier. 2. Insert the corresponding dichroic mirror. 2. Orient the UV protection screen so that you cannot look directly into the UV light.
Transmitted-light Base TL BFDF: Before First Use Removing the transport anchors Before you can use the transmittedlight base for the first time, it is absolutely necessary to remove the two transport anchors.
Transmitted-light Base TL BFDF Standard delivery The base is delivered with the adapter plate installed. The selected stage (Leica IsoPro™ mechanical stage or Leica standard stage 10 447 269), and the focusing drive will have to be mounted later. Stage assembly The Leica TL BFDF transmitted-light base can be equipped with three different stages. The selected stage is mounted on the base before commissioning. You can switch between the stages at any time with just a few hand movements.
TL RC™ / TL RCI™ The base is delivered with the adapter plate installed. The selected stage (Leica IsoPro™ mechanical stage or Leica standard stage 10 447 269), and the focusing drive will have to be mounted later. Ensure that the instruments are unpacked on a level, adequately sized, and nonskid underlay. Stage assembly The Leica TL RC™/ RCI™ transmitted-light base can be equipped with three different Leica stages. The selected stage is mounted on the base before commissioning.
IsoPro™ Manual Mechanical Stage: Assembly Leica IsoPro™ Mechanical Stage Before the Leica IsoPro™ mechanical stage is mounted to the base, the axis containing the control buttons is attached either on the left or the right side of the mechanical stage. Before the Leica IsoPro™ mechanical stage is mounted to the base, the axis containing the control buttons is attached either on the left or the right side of the mechanical stage.
Leica IsoPro™ Manual Mechanical Stage: Assembly (cont'd.) 1. Take the glass plate from the mechanical stage and turn it around. 2. Change the gear rod from the left to the right-hand side. Control assembly 1. Take the glass plate from the mechanical stage and turn it around. 2. Attach the axis with the control buttons to the desired side. The fastener snaps into the mechanical stage magnetically. Mechanical stage assembly 1. Place the mechanical stage onto the base. 2.
Leica IsoPro™ Manual Mechanical Stage: Assembly (cont'd.) 3. Attach the mechanical stage evenly to the three threaded holes. 4. Now move the mechanical stage as far as it will go in the direction of the column. 5. Insert the glass plate into the mechanical stage. Focusing drive and column 1. Unscrew the extension plate from the base using the Allen key provided. 2. Attach your focusing drive column from below using the three hexagon-head screws. 3.
Leica IsoPro™ Motorized Mechanical Stage: Assembly Basics The transmitted light bases of the Leica TL series (TL BFDF, TL RC™, TL RCI™) are supplied with an installed extension plate. The selected stage (Leica IsoPro™ mechanical stage or standard stage 10 447 269) and the focusing drive will have to be mounted later. 1. Unpack the mechanical stage from the transport packaging and position it on the transmitted-light base. 1 The motorized mechanical stage is a sensitive precision instrument.
Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.) 3. Remove the two M3 screws and the holder from the mechanical stage. 4. Remove the two M4 screws and the sleeve from the mechanical stage. 5. Remove the four shock-absorbing cartons from the mechanical stage. After removal, keep all the transport anchors in the plastic bag provided for future transport.
Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.) The bases The TL BFDF, TL RC™ and RCI™ transmitted-light bases can be equipped with three different stages: Standard stage, manual and automated IsoPro™ mechanical stage. The selected stage is mounted on the base before commissioning. You can switch between the stages at any time with just a few hand movements. The following paragraph assumes use of the base without the stage mounted.
Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.) Mechanical stage for X-Y stage control module 1. Plug the CTL2 plug of the mechanical stage motor into one of the three available CTL2 interfaces. As soon as all instruments have been plugged into the control box and it has been plugged into the power supply, the mechanical stage initializes and automatically drives to the mid position. 2. Plug the CTL2 plug of the Leica PSC controller into another CTL2 interface. 4.
Cables: Connections The new Leica M-series features extensive encoding with which various microscope data and settings can be read out, transferred to the PC and reproduced later. ρρ The interface to the optics carrier is on the column. The terminals The connection to the PC and to other instruments is made using the terminals on the rear side of the column: 1 3 2 1. 2 CTL2 terminals for auxiliary equipment such as the ring illuminator, the base and other accessories from the Leica product range. 2.
Cables: Cable Duct The integrated cable duct in the column enables a neat cable layout around the microscope. For example, the USB or FireWire cables of the camera can be stowed in the cable duct. Leica M-series Feeding the cables 1. Unscrew the three screws on the cable duct. 3. Place the cables in the cable duct and screw the cover on tightly. 2. Remove the cover of the cable duct. Tip: Estimate the length of the cable ends you will need before screwing on the cover.
Cables: Diagram Alternative installation Please use the Leica Application Suite to configure the functions of the system.
Leica LED5000 MCI™ The Leica LED5000 MCI™ (for "Multi Contrast Illumination") is installed using two screws. For optimum accessibility, the optics carrier should be removed during assembly (see Page 23.) Assembly 1. Hold the LED5000 MCI™ with one hand and tightly screw the retaining stirrups on both top holes on the drive housing. • 2. Connect the CAN-bus cable provided to either of the two sockets. (The flat part of the plug must be facing downwards.) 3.
Leica LED5000 MCI™: Alternative Assembly Under certain circumstances, the light source must not be moved along with the optics carrier. A typical example is multifocus images in which the Z-stack changes while the angle of incidence of the light must remain the same. For such purposes, the Leica LED5000 MCI™ is directly fastened to the column. 2. Screw the retaining stirrup into the column at the notch using the single screw.
Leica LED5000 RL The ring illuminator, the Leica LED5000 RL ("Ring Light"), is installed on the objective using a single screw. It has been optimized for a working distance between 60 and 70 mm. Constraints The Leica LED5000 RL can be used in conjunction with the planapochromat 1× and planapochromat 0.63× objectives. With all other objectives, the working distance is too low for adequate illumination. Assembly 1. Connect the CAN-bus cable to the ring illuminator.
Leica EL6000 – Safety Notes • sibility or liability for any use outside of The manufacturer assumes no respon- the intended use or use outside of the specifications from Leica Microsystems Wetzlar GmbH or any risks resulting from such use. In such cases, the Declaration of Conformity shall be invalid. • in the patient environment defined by This (IVD) device is not intended for use DIN VDE 0100-710. It is also not intended to be combined with medical devices as defined by EN 60601-1.
Leica EL6000 – Safety Notes (cont'd.) • generates high-energy light with invisi- • unit contains highly toxic substances. • the listed rated current may be used as The Leica EL6000 compact light source The interior of the lamp inserted into the Only fuses of the specified type and ble components. There is a risk of being dazzled or blinded by light! Never look directly into the light guide output of the instrument or into the output of the light guide connected to the instrument.
Leica EL6000 – Safety Notes (cont'd.) Electrical safety For indoor use only. Supply voltage 100–240 V~ (±10%) Frequency 50–60 Hz Power consumption Max. 210 VA Fuses 5×20, 2.
Leica EL6000 – Preparations Setting up the instrument Set up the Leica EL6000 so that the front is readily accessible and visible. • ρρ The ventilation slots on the sides and back panel of the device must not be covered. ρρ Maintain a free room of at least 150 mm on the rear panel of the instrument. Instructions for lamp replacement The lamp contains mercury! Be absolutely certain to follow the handling instructions and safety notes provided with the lamp.
Leica EL6000 – Preparation (cont'd.) 4. Carefully lay the Leica EL6000 on its side so that the opening faces you. 5. Pull the pressure bolt back towards the front plate using the lever. This makes it easier to insert the lamp. 6. Insert the lamp. Leica M-series User Manual Ensure that the groove in the contact surface of the lamp matches up with the corresponding plug in the lamp mount.
Leica EL6000 – Connection to the Fluorescence Lamp Housing • lamp housing of the stereomicroscope Always connect the light guide to the in order to prevent danger to the user from the high-energy light. 2. Insert the adapter into the fluorescence lamp housing and retighten the locking screw. 4. Insert the short end of the light guide into the lamp housing of the stereomicroscope as far as it will go. 5. Securely tighten the clamping screw. 1.
Leica EL6000 – Preparation (cont'd.) 7. When the lamp makes full contact with the lamp mount, release the lever. 9. Check to make certain that a heat-absorbing filter has been inserted into the shaft in order to protect the connected light guide. 8. Connect the plug of the lamp with the coupling in the instrument. Ensure that no wires are touching the reflector of the lamp. Leica M-series 10. Close the housing cover and insert the screws.
Leica EL6000 – Replacing the Lamp The procedure for replacing the lamp does not differ from the installation procedure outlined on Page 57, except for the fact that the existing lamp has to be removed first. 3. Remove the housing cover. Replacing the lamp The lamp used in the instrument becomes very hot during operation and has high internal pressure when hot. Before changing the lamp, it is mandatory to allow the instrument to cool off for at least 20 minutes. This makes it easier to insert the lamp.
Leica EL6000 – Connection to the Fluorescence Lamp Housing (cont'd.) 6. Insert the long end of the light guide into the light output of the Leica EL6000. There must be a noticeable click. • of the type "Storz long", as otherwise Only use a light guide with a light input damage to the instrument and danger to the user can result (risk of being dazzled). • guide must be connected on both Before you open the shutter, the light sides.
Quick Start Guide Leica M-series User Manual 63
The Fastest Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. Therefore, your next step should be to study the Quick Start Guide, which outlines the most important steps at a glance. This manual will then familiarize you with the finer details of your microscope. The following pages contain important, practical information that makes using it every day easier.
Overview of an M Series Microscope 1 2 3 4 5 6 7 8 9 Eyepieces for spectacle wearers with dioptric correction and eyecups Trinocular tube Fluorescence housing Filter Changer Display Motorized Focus Objective Nosepiece Leica SmartTouch UV protection screen 1 2 3 5 7 4 6 8 9 Leica M-series User Manual 65
The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry—after a little while, it will become automatic. Adjusting the interpupillary distance 1. Look into the eyepieces. 3. Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting. 2.
Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer. Simply push them into the tube and they are ready to use. If you do not wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise. 2. If an eyepiece is equipped with the inte- If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups clockwise towards the rear, as otherwise the viewing distance is too great.
Focusing Focusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. ρρ The focusing drive can be operated either left- or right-handed. Leica M-series Focusing ρρ The inner, coarse adjustment is used for covering great distances. ρρ The outer, fine adjustment is used for fine focusing. User Manual Coarse/fine adjustment The sharpness is adjusted using the coarse/fine adjustment.
Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depending on the equipment weight and personal preferences as follows: 1. Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Changing Magnification (Zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range covered: ρρ Leica M165 C = 16.5:1 ρρ Leica M205 C = 20.5:1 Zooming 1. Look into the eyepieces. 2. Focus on the specimen. 3. Rotate the magnification changer until the desired magnification is configured. The rotary knob for the zoom can be used either left or right-handed.
Ratchet Steps and Magnification Levels The zoom button can optionally be operated either with or without ratchet steps. Continuous zoom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs, measurement results etc. can be reproduced more accurately. Leica M-series Enabling and disabling ratchet steps 1. Push the top button downwards to disable the ratchet steps. 2.
Parfocality: More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magnification without having to refocus. The focus needs only be readjusted if you want to see a specimen location that is located higher or lower. Parfocality 1. Enlarge the view to the maximum level.
Iris Diaphragm The iris diaphragm in the optics carrier of your M series microscope fulfills the same purpose as the iris diaphragm in a camera: it regulates the available light, which changes the depth of field. The "depth of field" (or "focus depth") is the area of a specimen that is brought into sharp focus. Leica M-series Closing the iris diaphragm ρρ Close the iris diaphragm by turning the knob to the left. The subject appears darker and the depth of field increases.
Eyepieces Leica M-series User Manual 74
Magnification Factors of the Eyepieces An eyepiece not only makes it possible to look passively into the microscope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x.
Health Notes Potential sources of infection Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately. Please contact your Leica partner. • Separate eyecups are an effective way of preventing infections.
Dioptric Correction All Leica eyepieces are also available with builtin dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Correction 1. Set the dioptric correction of both eyepieces to the mid position ("0" diopter settings). 2. While wearing your glasses, look through the eyepieces and focus on the specimen.
Dioptric Correction and Parfocality Leica stereomicroscopes are parfocally matched. The prerequisite for this is the correct setting of the diopters and the parfocality. The following adjustments only have to be carried out once by each user. Adjusting 1. Set the dioptric correction for both eyepieces to "0". Preparations ρρ Move the lever of the video/phototube to the "observation" position and open the diaphragm. 3. Select the highest magnification and readjust the sharpness.
Graticules Use Leica graticules make length measurements and counting easier, particularly for workstations that are not equipped with a digital camera and LAS software. 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the graticule fits tightly. 4. You can now align the graticule by rotating the eyepiece in the tube and then tightening it using the clamping screw. 3. Screw the insert and graticule firmly into place and replace the eyepiece in the tube.
Photography & Video Leica M-series User Manual 80
Photography & Video For most microscope users, digital documentation has become an invaluable part of their work. Research results can be presented in an attractive manner; measurements on the digital image provide clarity and, in conjunction with the motorized IsoPro™ mechanical stage, even images of large specimens can be captured step by step and automatically joined to create a new complete image.
Photo Tubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount interface. In turn, the C-mount adapter for the respective trinocular tube is connected to this interface. This adapter creates a solid mechanical connection between the microscope and camera and ensures optimum rendering of the microscopic image on the image sensor of the camera.
Trinocular Video/Phototube 50% Use With its third beam path, the trinocular video/ phototube 50% enables you to simultaneously view and photograph a specimen. The available light is divided as follows: ρρ 50% is available for the two eyepieces. ρρ 50% of the light is diverted to the video/ photo beam path. Assembly Fasten the "trinocular tube 50%" to the optics carrier instead of the binocular observation tube (refer to Page 24).
Trinocular Video/Phototube 100% Use With its third beam path, the trinocular video/ phototube 100% enables you to either view or photograph a specimen. This means that 100% of the light is available to one or the other beam path. The other beam path remains opaque or black. Assembly Fasten the "trinocular tube 100%" to the optics carrier instead of the binocular observation tube (refer to Page 24).
Microscope Carrier Leica M-series User Manual 85
The Objective Nosepiece Use The objective nosepiece enables you to switch between two objectives using just one hand movement, for example between a 1× and a 2× planapochromat. Parfocal work With the new M series objectives, the parfocality is maintained even when objectives are changed, meaning that the specimen remains in sharp focus during the change.
Objectives and Optical Accessories Leica M-series User Manual 87
The Different Types of Objectives To meet the various requirements regarding imaging properties, there is a choice of highquality interchangeable planachromatic and planapochromatic objectives and also lowerpriced interchangeable achromatic objectives. ρρ Achromatic objectives are particularly suited for specimens with high-contrast structures. ρρ Flat-field (planachromatic) objectives are particularly well suited for studying flat objects such as wafers and thin sections. Achromatic objectives The 0.
Bases Leica M-series User Manual 89
Leica TL ST Transmitted-light Base: Controls 1 2 1 2 3 3 4 1 2 3 4 Extension plate of the transmitted-light base TL ST Adapter plate for easy assembly of focusing drives Removable glass plate Controller for light intensity Adjustment for deflection mirror Leica M-series User Manual Rear side of the transmitted-light base TL ST 1 Screws for changing the halogen lamp 2 Power connection socket 3 Power switch 90
Leica TL ST Transmitted-light Base: Operation Light intensity control The left control adjusts the intensity of the 12 V/20 W halogen illumination. 1. Switch on the illumination of the base at the power switch. Transmitted-light control The transmitted-light base TL BFDF has a slider that automatically moves the deflection mirror in the base when moved. The mirror is kept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light. 2.
Leica TL ST Transmitted-light Base: Changing Bulbs Changing the halogen lamp Before you change the lamp, it is absolutely necessary to unplug the power plug from the base to prevent the risk of electric shock! Changing bulbs 1. Unscrew the two screws on the heat sink and pull the heat sink out, along with the lamp. Precautionary measures When inserting the lamp, ensure that the cables are inside the two metal clamps. This prevents the cables from getting caught during insertion.
Leica TL BFDF Transmitted-light Base: Controls 1 2 Adapter at the focusing drive 3 1 2 3 Extension plate of the Transmitted-light base TL BFDF Adapter plate for easy assembly of focusing drives Standard stage 10 447 269 Button to toggle between bright field and dark field Button to toggle between bright field and dark field Leica M-series User Manual Connector for cold light sources (light conductor active f = 10mm, end tube f = 13mm) 93
Leica TL BFDF Transmitted-light Base: Operation Light intensity control Observe the user manual—in particular, all safety regulations—from the manufacturers of the light guide and cold light source. • ρρ Switch on the cold light source according to the manufacturer's user manual and adjust the brightness. Bright field Bright field is suitable for examining translucent objects featuring contrasting structures.
Leica TL RC™ / TL RCI™: Controls 1 2 1 2 3 4 5 6 7 3 45 Vertical column - transmitted-light base adapter plate 1 2 3 4 5 6 7 Heat sink of the integrated halogen illumination (only TL RCI™) Extension plate for focusing drives Standard stage 10 447 269 Filter holder Control of top and bottom flaps of the Rottermann Contrast™ Button for mirror and horizontal movement of the mirror Transmitted-light base Leica M-series User Manual 1 2 3 4 5 6 6 Power switch Power connection socket USB socket, type B
Leica TL RCI™: The Deflection Mirror 1 Inverted world? Depending on the properties of the specimen (refractive index of the environment) and the perception of the viewer, it is possible that the switches described below for positive and inverted relief contrast are to be operated in reverse. This means that the lower switch, rather than the upper switch, controls inverted the relief contrast and vice-versa.
Leica TL RCI™: Color Intensity and Temperature The transmitted-light base TL RCI™ has two electronic potentiometers that control the color intensity (1) and color temperature (2). The controller for the color temperature simultaneously serves as an electronic shutter: ρρ To interrupt your work, click the potentiometer (2).
Leica TL RC™ / TL RCI™: Operation Setting the relief contrast The two switches on the left side of the transmitted-light base TL RC™/TL RCI™ activate two built-in flaps. The outer switch (1) controls the inverted relief contrast, and the inner switch (2) adjusts the positive relief contrast. 1 1 2 3 2 Depending on the flap position, a part of the opening of the built-in Fresnel lenses are covered, which results in the different contrast effects.
Leica TL RCI™: Methods in Transmitted Light Vertical bright-field illumination Suitable for stained amplitude specimens with sufficient contrast. The light beams are deflected vertically through the specimen. This results in an accurate bright field with maximum brightness. Leica M-series Inclined transmitted light Suitable for semitransparent, opaque specimens such as foraminifera and fish eggs. Move the deflection mirror until the desired data are visible.
Leica TL RCI™: Relief Images Starting position 1. Push the deflection mirror all the way back towards the column. 2. Turn the deflection mirror into the notch position at an angle of 45°. Leica M-series Positive relief contrast Suitable for semitransparent and transparent specimens. The phase structures look like hills. The effect can be strengthened or weakened by gently tilting the deflection mirror. Negative relief contrast Suitable for semitransparent and transparent specimens.
Leica TL RCI™: Relief Images (cont'd.) Dynamic relief contrast Suitable for semitransparent and transparent specimens. By tilting the deflection mirror slightly, you can move the gaps over the entire field of view and quickly toggle between positive and negative relief images. The dynamic effect makes it easy to distinguish phase structures from amplitude structures. Leica M-series Constraints The relief methods provide good results from mid-zoom to high magnifications and with 1×, 1.6× and 2× objectives.
Using Filters Filters for Leica TL RC™ and TL RCI™ The transmitted-light bases TL RC™ and TL RCI™ can be equipped with up to three filters— available as accessories—simultaneously. By customer request, the filters are also available as one-off items. 2. Take the empty filter from an available filter slot in the filter holder. Daylight filter for Leica TL ST A daylight filter is also available for the Leica TL ST transmitted-light base. 3. Insert the desired filter. 1.
Leica IsoPro™ (Non-motorized): Controls Operating the Leica IsoPro™ mechanical stage 1. To move the stage in the X direction, rotate the outer knob. 2. To move the stage in the Y direction, rotate the inner control ring.
Leica IsoPro™ (Motorized): Controls IsoPro X/Y Stage DCI module 1 Leica PSC Controller 1 2 2 3 1 1 2 Leica IsoPro™ motorized mechanical stage Housing with motorization Leica M-series 1 2 3 4 2 3 4 Sub-D interface for Leica SmartMove™ 3 CTL2 interfaces USB interface (type B) Socket for grounded power cable User Manual 1 2 3 Quick control/memory function Fine control in X direction Fine control in Y direction 104
System Illumination Leica M-series User Manual 105
Leica LED5000 MCI™ Use The Leica LED5000 MCI™ (for "Multi Contrast Illumination") is a universal high-output illuminator. Three groups of 3 LEDs each can be arranged as desired around the specimen. The targeted control of the LEDs enables various illumination scenarios to be implemented. Preparation Hold the MCI with both hands and pull it downwards until the illuminator clicks into place on the bottom end of the guide rods.
Leica LED5000 MCI™ (cont'd.) Using the keyboard ρρ Use the key to switch the illumination on or off. The illumination scenarios Use the " " and " " keys to toggle between the illumination scenarios shown below. ρρ Use the "+" and "–" keys to adjust the brightness in 10 increments. Touch either of the two buttons to adjust the intensity in small increments. Hold a key to change the intensity more quickly.
Leica LED5000 RL Use The Leica LED5000 RL ("Ring Light") generates a very bright and homogenous incident light. It has a diameter of 80 mm and illuminates the specimen with 48 LEDs that can be switched on and off completely or in various combinations. It is controlled using either the integrated or via the Leica Application Suite (LAS). Using the keyboard ρρ Use the key to switch the illumination on or off. ρρ Use the "+" and "–" keys to adjust the brightness in 10 increments.
Leica EL6000 – About the Instrument The Leica EL6000 compact light source is intended for biological routine and research work, including the examination of specimens from the human body for the purpose of gaining information. • source The Leica EL6000 compact light is intended exclusively for operating mercury short-arc lamps of type HXP-R120W/45C VIS from OSRAM in conjunction with a suitable light guide for the instrument. Any other use of the instrument shall be considered noncompliant use.
Leica EL6000 – About the Instrument (cont'd.) Compatible light guides The Leica EL6000 compact light source is compatible with liquid light guides that are compatible with a "Storz long" light inlet and whose transmission curve fits the lamp used. Where applicable, refer to the lamp's data sheet for its spectral light distribution. Identification of the instrument The nameplate of the instrument is on its bottom. It includes the necessary information for identifying the instrument.
Leica EL 6000 – Operation Controls Running-time meter The running-time meter measures the burning time of the lamp, not the switch-on time of the Leica EL6000 compact light source. The running-time meter displays its current status in this format: hours – minutes. 1. Press the reset button to set the runningtime meter of the lamp to "0". 5 The running-time meter should be reset after every lamp replacement so that it always displays the current, accurate lamp life.
Leica EL 6000 – Troubleshooting Fault description Power indicator is not illuminated after being switched on, instrument shows no function Power switch is illuminated after being switched on, instrument shows no function Target system receives too little light or none at all Possible faults Remedy No power supply voltage present; connecting cable not plugged in correctly/defective Check power supply voltage and connecting cable Fuses in instrument defective Replace fuses, use permitted replacement
Cleaning and Maintenance • switch off the Leica EL6000 compact Before carrying out cleaning tasks, light source. Spare parts and accessories The only parts of the Leica EL6000 compact light source that may be replaced by the user are the instrument fuses and the lamp. Fuse: 5×20, 2.5 A, slow-blowing, breaking capacity H Wickmann Series 181 www.wickmann.com Schurter Series SPT 5×20 www.schurter.ch Use only a dry, lint-free cloth for cleaning.
Fluorescence Leica M-series User Manual 114
Filter Changer The patented FLUOIII® filter system consists of a filter changer for barrier and excitation filters, a UV shutter that can be activated/deactivated, and a filter insert for a filter holder with your choice of filter. The filter changer can hold a total of 4 filter sets. The filter sets for fluorescence are labeled (see Page 118). All filter sets are equipped with a transponder for automatic filter detection and can also be used with the motorized Leica M205 FA.
FIM – Fluorescence Intensity Manager FIM consists of a diaphragm disk with pinhole diaphragms of varying transmission capacities. The intensity of the excitation light can be reduced in five fixed steps. The light can be changed from 100% to 55%, 30% and 17% down to 10%. The advantage of this procedure is its absolute reproducibility and fast switching between the intensity levels. A separate FIM level can be stored for every fluorescence filter.
About Fluorescence Microscopy Functional principle Fluorescent substances light up when irradiated with short-wave excitation light, and fluorescence microscopy takes advantage of this property. Specimens without self-fluorescence are stained with a fluorescent substance. One such dye is green fluorescent protein (GFP), which is used in molecular biology.
Rapid Filter Changers and Filter Types The rapid filter changer must be equipped with four filter holders at all times.
Simple filter holders Simple filter holders Three simple filter holders are included with the Leica M165 FC and the Leica M205 FA. These filter holders have two empty openings for the observation beam paths. The third opening, to the illumination beam path, is closed. ρρ Use this filter holder if you want to work briefly (no more than 15 seconds) without fluorescence illumination. So that the filter holder is not heated, it must not block the mercury light for longer than 15 seconds.
Equipping the Filter Changer • touching the filters in order to avoid When inserting the filter sets, avoid fingerprints. Clean dirty filters immediately using a soft, lint-free cloth and pure alcohol. Removing the simple filter holder The Leica M165 FC and the Leica M205 FA are equipped at the factory with simple filter holders as "dummies". These must be removed before inserting a filter. Inserting the filter 1. Place the filter into the filter changer. 1.
Observation without Fluorescence We recommend that you first familiarize yourself with the controls of your Leica fluorescence microscope in transmitted light without the presence of fluorescence illumination. • mercury burner, you must adjust the arc After switching on the high-pressure lamp immediately (see User Manual M2-216-1). We recommend that you first familiarize yourself with the basic functions of your Leica stereomicroscope as they are described in the previous section of this User Manual.
Double Iris Aperture The built-in double-iris diaphragm regulates the depth of field. Note that: ρρ The greater the depth of field, the darker the image becomes. ρρ In the same way, the exposure time becomes longer for photographs. ρρ The resolution decreases.
Commissioning the Fluorescence System After switching on the high-pressure mercury burner, you must adjust the arc lamp immediately (see User Manual M2-216-1). Do not switch on the supply unit until you feel confident using the Leica stereomicroscope. • always position the protection screen For observation with fluorescence light, correctly to protect the observer (see Page 27). • handling mercury lamps and the lamp Follow all safety instructions when housing! 1.
Accessories Leica M-series User Manual 124
Leica SmartMove With its non-slip controllers and knobs, the Leica SmartMove control unit offers the right feel for handling motorized Leica instruments. Controls The functions of the individual controls can be configured both directly on the Leica SmartMove and using the Leica Application Suite. For a detailed description of the functions and settings, please refer to the Leica SmartMove manual or the Leica Application Suite help file.
Leica SmartTouch™ With the touchscreen of the Leica SmartTouch™, you can control your experiment procedures with a few hand movements and never lose sight of all your important optical parameters. The most important control functions on the control unit can be adjusted to your specific needs with freely programmable rotary knobs and function buttons.
Leica PSC Controller Use The Leica PSC controller gives you precision control of the Leica IsoPro™ motorized mechanical stage. Passing over the specimen quickly ρρ Move the joystick in any desired direction (including diagonally) to control the mechanical stage quickly. Control by LAS The instructions for controlling the Leica IsoPro™ using the Leica Application Suite are provided in the software's help file.
Dimensional Drawings Leica M-series User Manual 128
547 431 Leica M165 FC Leica M165 FC with incident light base and new focusing column (dimensions in mm) 154 95 116 154 385 547 154 95 116 154 440 390 431 292 217 340 385 Leica M-series User Manual 129
431 547 154 187 116 Leica M165 C with TL RCI™ transmitted light stand, Leica IsoPro™ manual mechanical stage and manual focusing drive 497 95 Leica M165 FC (cont'd.) max. 370 154 497 187 X = +-75 mm 95 116 370 max.
431 537 Leica M165 FC (cont'd.
431 547 Leica M205 FA 154 292 90 116 Leica M205 FA with TL BFDF transmitted light stand, ErgoTrinoc tube, DFC camera and motorized focusing drive154 547 431 390 370 340 154 90 116 154 370 Leica M-series User Manual 132
431 547 Leica M205 FA (cont'd.
547 431 Leica M205 FA (cont'd.) 95 116 Dimensions of Leica M205 FA with TL RCI™ transmitted light stand, IsoPro™497 mechanical stage, ErgoTrinoc tube, DFC camera and motorized focusing drive 154 187 292 154 497 187 X = +-75 mm 95 116 370 max. 410 Leica M-series User Manual 134 300 249 547 431 Y = +-50 mm max.
45 90 112 157 Leica LED5000 MCI 45 Leica LED5000 MCI (dimensions in mm) 45 132 83.5 36 36 112 112 22 22 132 90 90 112 112 157 157 45 45 275.
ø70 ø80 153 153 Leica LED5000 RL 30 120 Leica M-series User Manual 136
98 170 134 MATS TL 140 190 220 11 4 10 447 275 10 447 164 Leica M-series User Manual 137
Specifications Leica M-series User Manual 138
Leica M165 FC / Leica M205 FA Leica M165 FC Leica M205 FA Zoom 16.5 : 1 manual 20.5 : 1 automated with FusionOptics™ Data with standard optics (1×objective/10× eyepieces) – Zoom range – Resolution – Working distance – Object field 7.3×–120× max. 453 lp/mm 61.5 mm (planapochromat) ∅ 31.5 mm – 1.92 mm 7.8×–160× max. 525 lp/mm 61.5 mm (planapochromat) ∅ 29.5 mm – 1.
Leica M165 FC / Leica M205 FA (cont’d.
Leica M165 FC / Leica M205 FA (cont’d.) Leica M165 FC Leica M205 FA Additional objectives (non-parfocal) 1× planachromat, 0.8× planapochromat, 0.63× planapochromat, 0.5× planapochromat 1× planachromat, 0.8× planapochromat, 0.63× planapochromat, 0.
Leica M165 FC / Leica M205 FA (cont’d.) Leica M165 FC Leica M205 FA Incident light stand Large incident light base with black/white stage plate and Antishock™ feet Stages Gliding stage, MATS heating stage, cup stage, rotatable polarization stage, Leica IsoPro™ m echanical stage (manual and motorized) Illuminators LED5000 MCI™ and LED5000 RL, fluorescence, oblique, coaxial, cold light sources Misc.
Leica EL6000 – Specifications Dimensions (W×H×D) Width×height×depth Environmental conditions Permitted ambient temperature Permitted relative humidity 130 mm × 210 mm × 290 mm -20 to 85 °C 10% to 90%, non-condensing Operation Permitted ambient temperature 0 to 40 °C Permitted relative humidity 10% to 90%, non-condensing Operational data Application area Enclosed rooms Altitude for use Max.
Leica EL6000 – Specifications (cont'd.) Light output Design Special feature Fan Switch-on delay Interface Type Design Remote signal level – Shutter closed – Shutter open – Input current Footswitch – Shutter closed – Current Shutter frequency Leica M-series Type: Storz, long Self-locking if no light guide is plugged in Approx.
Leica TL ST Transmitted-light Base Light source Quick illuminant change Illuminated area Power supply unit Connections Weight Illumination types Bright field Dark field Oblique light Relief Contrast System (RC™) CCIC (Constant Color Intensity Control) Internal shutter/lamp control Integrated filter holder Coated optics for increasing the color temperature Matching of high num.
Leica TL BFDF Transmitted-light Base Light source Illuminated area Connections Weight External via cold light source 40 mm Connection for cold light guide, active f=10mm, end tube f=13mm 5.8 kg Illumination types Bright field Yes Dark field Yes Oblique light No Relief Contrast System (RC™) No CCIC (Constant Color Intensity Control) No Internal shutter/lamp control Yes* Integrated filter holder No Coated optics for increasing the color No temperature Matching of high num.
Leica TL RC™ / TL RCI™ Light source Quick illuminant change Illuminated area Power supply unit Connections Weight Leica TL RC™ External via cold light source – 35 mm – Connection for cold light guide, active f=10mm, end tube f=13mm 6.0 kg Leica TL RCI™ Halogen lamp 12V/20W Yes 35 mm 100–240 V~, frequency 50–60 Hz energy consumption 30 W max., ambient temperature 10 – 40 °C 1×USB type A, 1×USB type B, 2×CAN-BUS 7.
Leica IsoPro™ Motorized XY Stage Compatibility Leica incident light base and TL bases (TL BFDF, TL RC™, TL RCI™) Stage size (L × W × H) 335.5 mm × 370 mm × 41.5 mm Travel 152 mm × 102 mm (6" × 4") Speed X,Y 30 mm/s Motor resolution X,Y 0.
Appendix Leica M-series User Manual 149
Calculating the Total Magnification and Field of View Diameter Parameter Example MO Magnification of the objective MO 1× objective ME Magnification of the eyepiece ME 25×/9.5 eyepiece z Magnification of the changer position z Zoom position 4 q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™ q Coaxial reflected light 1.5×, tube factor r Factor 1.25× r Factor 1.
Care, Maintenance, Contact Persons We hope you enjoy using your high-performance microscope. Leica microscopes are renowned for their robustness and long service life. Observing the following care and cleaning tips will ensure that even after years and decades, your Leica microscope will continue to work as well as it did on the very first day. Warranty benefits The warranty covers all faults in materials and manufacture. It does not, however, cover damage resulting from careless or improper handling.
Care, Maintenance, Contact Persons (cont'd.) Protection from dirt Dust and dirt will affect the quality of your results. ρρ Put a dust cover over the microscope when it will not be used for a long time. Cleaning polymer components Some components are made of polymer or are polymer-coated. They are, therefore, pleasant and convenient to handle. The use of unsuitable cleaning agents and techniques can damage polymers.
