Leica M50 Leica M60 Leica M80 User Manual
General Instructions Safety concept Before using your microscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains additional information about handling and care. Use in clean rooms The Leica M series can be used in clean rooms without any problems. Cleaning ★★ Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning. ★★ Never use chemicals to clean colored surfaces or accessories with rubberized parts.
Important Safety Notes User Manual The individual modules of the Leica M stereomicroscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.stereomicroscopy.com.
Symbols Used Warning of a danger This symbol indicates especially important information that must be read and complied with. • Danger due to hot surface. This symbol warns against touching hot surfaces, e.g. those of light bulbs. Failure to comply can cause the following: ★★ Hazards to personnel ★★ Functional disturbances or damaged instruments Important information This symbol indicates additional information or explanations that are intended to provide clarity.
Safety Regulations Description ★★ The individual modules fulfill the highest requirements for observation and documentation of Leica stereomicroscopes of the M series. Designated use ★★ Refer to "Safety Concept" booklet Non-intended use ★★ Refer to "Safety Concept" booklet Never use M series microscopes or their components for surgical procedures (e.g. on the eye) unless they are specifically intended for that purpose.
Safety Regulations (cont'd.) Repairs, service work ★★ Refer to "Safety Concept" booklet Installation in third-party products ★★ Refer to "Safety Concept" booklet ★★ Only original Leica Microsystems spare parts may be used. Disposal ★★ Refer to "Safety Concept" booklet ★★ Before opening the instruments, switch off the power and unplug the power cable. Legal requirements ★★ Refer to "Safety Concept" booklet ★★ Touching the live circuit can cause injury.
Safety Regulations (cont'd.) Light sources: safety regulations Protective measures of the manufacturer: ★★ UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays. Warning ★★ UV radiation could damage the eyes. Therefore: ★★ Never look into the light spot on the specimen plane without a UV protection screen.
Safety Regulations (cont'd.) Lamp housing ★★ Never open the lamp housing while the lamp is switched on. Risk of explosion, UV exposure, blinding! ★★ Before opening the lamp housing, allow it to cool off for at least 15 minutes. Danger of explosion! ★★ Never cover the air duct on the lamp housing. Danger of fire! Mercury lamp ★★ Follow the user manual and safety regulations provided by the lamp manufacturer, and, in particular, the section on how to proceed if a lamp breaks and releases mercury.
Table of Contents Inhaltsverzeichnis General Instructions Important Safety Notes Symbols Used Safety Regulations Safety Regulations (cont'd.) Safety Regulations (cont'd.) Safety Regulations (cont'd.
Photography & Video Photography & Video Photo Tubes and C-mounts Video/Phototubes HD F & HD V Objectives Optical Accessories The Different Types of Objectives 44 45 46 63 64 65 and 48 System Illumination Leica LED3000 NVI™ (Near Vertical Illumination) Leica LED5000 CXI (Coax Illumination) 50 51 Dimensional Drawings Leica M50 Leica M50 Leica M50 Leica M60 Leica M80 Leica M80 Leica M80 Leica M80 Leica M80 53 54 55 56 57 58 59 60 61 Leica M-series Appendix Calculating the Total Magnific
The Leica M series Leica M-series User Manual 11
Congratulations! The New Leica M Series The introduction of the Leica M50, M60 and M80 adds three new highquality routine stereomicroscopes to the proven M-Series range from Leica Microsystems. The optical brilliance and the wide range of accessories make them the ideal microscope for quality assurance and similar industrial applications.
The Modular Design: Everything is Relative The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades. The optics carriers, eyepieces, bases and more can be combined in any way you choose, allowing you to create the microscope that best suits your needs. Leica M-series Despite this, you will notice that the controls and individual components do not differ significantly.
Maximum Compatibility Leica engineers were careful to ensure that the new Leica M series—like its predecessors— remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused. Objectives All objectives of this new generation have the same reference focal length as the objectives of the MZ generation. If you prefer, you can therefore continue to use the previous Leica objective series.
On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on Page 29. Leica M-series If, on the other hand, you are assembling the microscope yourself, continue with the "Assembly" chapter, which begins on Page 16.
Assembly Leica M-series User Manual 16
Base and Focusing Column The first step is to connect the focusing column of the M series to the corresponding base. Assembling the column adapter 1. Remove the three screws provided from the column. 2. Install the base on the column using the three screws. Tools used ★★ Hex socket screwdriver, 3 mm Tip: Place the column on the edge of a table to attach the base and have another person help you.
Microscope Carrier Tools ★★ Allen screwdriver provided Alternative installation options Depending on the desired working distance, the microscope carrier can be installed in the standard way or upside down (see figure below). Assembly The microscope carrier is fastened to the column using the screw provided.
Optics Carrier Tools used ★★ None Assembling the optics carrier 1. Unscrew the clamping screw on the holder for the optics carrier. 2. Tighten the clamping screw below the optics carrier. 2. Place the optics carrier into the holder and align it in such a way that the notch in the optics carrier and the bottom clamping screw are on top of each other.
Tube All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner. Tools used ★★ No tools required. Assembling the tube 2. Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove. 3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position. Preparation 1.
Eyepieces Tools used ★★ No tools required. Magnification range You can extend the overall magnification range using available 10×, 16×, 25× and 40× widefield eyepieces for persons wearing glasses. Preparation 1. If you want to use an optional graticule, insert it now (Page 43). Inserting the eyepieces 3. Push the eyepieces into the tubes as far as they will go and check to ensure that they fit tightly and accurately. 2. Remove the plastic tube guard. 4. Securely tighten the clamping screws.
Objective Tools used ★★ No tools required. Preparation 1. Remove the protective cap on the optics carrier by turning it. Attaching the objective Hold the objective firmly during assembly and disassembly so that it does not fall onto the stage plate. This applies particularly to the 2x planapochromatic objective, which is very heavy. Remove all specimens from the stage plate first. • 2. Screw the objective clockwise into the optics carrier.
Leica LED5000 CXI (Coax Illumination) Required tools ★★ None Installing the Leica LED5000 CXI 1. Connect the Leica LED5000 CXI to the focusing column via the CTL2 connection if the focusing column is equipped with integrated electronics. 2. Unscrew the positioning screw and remove the protective cover. Alternative installation for manual columns 1. Connect the external power supply unit (10 450 266) to the Leica LED5000 CXI.
Leica LED5000 CXI (cont'd.) 3. Place the Leica LED5000 CXI on the optics carrier and tighten the positioning screw. Leica M-series 4. Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove. User Manual 5. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position.
Leica LED3000 NVI™ (Near Vertical Illumination) Required tools ★★ None The Leica LED 3000 NVI™ can be used with any objectives that have an outer diameter of 58 mm. Installing the Leica LED3000 NVI™ 1. Connect the Leica LED3000 NVI™ to the focusing column via the CTL2 connection if the focusing column is equipped with integrated electronics. Alternative installation for manual columns 1. Connect the external power supply unit (10 450 266) to the Leica LED3000 NVI™. 2.
Leica LED3000 NVI™ (cont'd.) • illuminator and the locking screw of the Ensure that the locking screw of the optics carrier are on a straight line, as otherwise image shading may occur.
Quick Start Guide Leica M-series User Manual 27
The Reliable Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. This manual will familiarize you with the finer details of your microscope. The following pages contain important, practical information that makes using it every day easier.
Overview of an M Series Microscope 1 2 1 Eyepieces for spectacle wearers with dioptric correction 3 2 Fastening screws for the eyepieces 3 Ergo tube 4 Optics carrier 5 Magnification changer (zoom) 6 Interchangeable objective 7 Incident-light base 8 Column 9 Cable duct with cover and eyecups 8 9 4 5 10 6 10 Coarse/fine focusing 7 Leica M-series User Manual 29
The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice microscope user, you may need a short time to become accustomed to this. Not to worry—after a little while, it will become automatic. Adjusting the interpupillary distance 1. Look into the eyepieces. 2. Hold the eyepieces with both hands. Push the eyepieces together or separate them until you see a circular image.
Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer. Simply push them into the tube and they are ready to use. If you do not wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise. If you wear glasses: 1. Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear, as otherwise the viewing distance is too great.
Focusing Focusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sharp focus as soon as it is in the focal point of the objective. ★★ The focusing drive can be operated either left- or right-handed. • load of up to 15 kg. The coarse/fine adjustment carries a The resolution of the coarse/fine adjustment is 1 µm.
Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focus movement too loose or too tight? Does the outfit tend to slide downwards? The resistance can be adjusted individually depending on the equipment weight and personal preferences as follows: 1. Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing.
Changing the Magnification (Zoom) All M series microscopes have an integrated zoom. The name indicates the zoom range covered: Zooming 1. Look into the eyepieces. 2. Focus on the specimen. ★★ Leica M50 ★★ Leica M60 ★★ Leica M80 = 5 increments = 6:1 zoom = 8:1 zoom 3. Rotate the magnification changer until the desired magnification is configured. The rotary knob for the zoom can be used either left or right-handed.
Ratchet Steps and Magnification Levels The zoom button can optionally be operated either with or without ratchet steps. Continuous zoom is possible when the ratchet steps are disabled, which many users find convenient. On the other hand, when the ratchet steps are enabled, photographs, measurement results etc. can be reproduced more accurately. Leica M-series Enabling and disabling ratchet steps 1. Push the button towards the knob to enable the ratchet steps. 2.
Eyepieces & Tubes Leica M-series User Manual 36
Magnification Factor of the Eyepieces An eyepiece not only makes it possible to look passively into the microscope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x.
Health Notes Potential sources of infection Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye. The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately. Please contact your Leica partner. • Separate eyecups are an effective way of preventing infections.
Dioptric Correction All Leica eyepieces are also available with builtin dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings. Using the Dioptric Correction 1. Set the dioptric correction of both eyepieces to the mid position ("0" diopter settings). 2. While wearing your glasses, look through the eyepieces and focus on the specimen. 3. Rotate both eyepieces to the maximum value of "+5". 4.
Dioptric Correction and Parfocality Leica stereomicroscopes are parfocally matched. The prerequisite for this is the correct setting of the diopters and the parfocality. The following adjustments only have to be carried out once by each user. Adjusting 1. Set the dioptric correction for both eyepieces to "0". Preparation ★★ Move the lever of the video/phototube to the "observation" position and open the diaphragm. 3. Select the highest magnification and readjust the sharpness. 2.
Graticules Use Leica graticules make length measurements and counting easier, particularly for workstations that are not equipped with a digital camera and LAS software. 2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the graticule fits tightly. 4. You can now align the graticule by rotating the eyepiece in the tube and then tightening it using the clamping screw.
ErgoTube 10° to 50° ErgoTube™ 10° to 50° The ErgoTube™ with continuously adjustable viewing angle from 10° to 50° allows for optimum sitting posture. Users who work with the same stereomicroscope can adjust the viewing height to their height with a simple hand movement. If the head posture is changed, the viewing height can also be readjusted without having to adjust the height of the chair. Special long tubes enable the user to maintain an upright posture.
Photography & Video Leica M-series User Manual 43
Photography & Video For most microscope users, digital documentation has become an invaluable part of their work. Research results can be presented in an attractive manner; measurements on the digital image provide clarity and, in conjunction with the motorized IsoPro™ cross-stage, even images of large specimens can be captured step by step and automatically joined to create a new complete image.
Photo Tubes and C-mounts Application All Leica DFC cameras are equipped with a standardized C-mount interface. In turn, the C-mount adapter for the respective trinocular tube is connected to this interface. This adapter creates a solid mechanical connection between the microscope and camera and ensures optimum rendering of the microscopic image on the image sensor of the camera.
Video/Phototubes HD F & HD V The video/phototubes HD F and HD V are optimized for adapting state-of-the-art digital cameras to obtain perfect results for image acquisition. The video/phototubes can be combined with the binocular tubes and the ErgoModules™ from the Leica ergonomics line. A range of high-quality video objectives with different self-magnifications and C-mount allows for the selection of different cameras.
Objectives and Optical Accessories Leica M-series User Manual 47
The Different Types of Objectives To meet the various requirements regarding imaging properties, there is a choice of highquality interchangeable planachromatic and planapochromatic objectives and also lowerpriced interchangeable achromatic objectives. Achromatic objectives The 0.32×, 0.5×, 0.63×, 0.8×, 1×, 1.5×, 2× achromatic objectives offer countless variants for selecting the object field diameter, magnification ranges and working distances (see page 63).
System Illumination Leica M-series User Manual 49
Leica LED3000 NVI™ (Near Vertical Illumination) The intensity of the illuminator can be adjusted in 10 increments. The Leica LED3000 NVI™ can also be controlled via the Leica Application Suite (LAS) or the Leica SmartTouch. The supported working distance is between 50 mm and 150 mm. Use • The light of the Leica LED3000 NVI™ can be very bright. Therefore, always switch on the illuminator before you look through the eyepieces! 2. Adjust the brightness by briefly pressing the or buttons. 1.
Leica LED5000 CXI (Coax Illumination) The intensity of the illuminator can be adjusted in 10 increments. The Leica LED3000 NVI™ can also be controlled via the Leica Application Suite (LAS) or the Leica SmartTouch. Using the Leica LED5000 CXI results in an increased magnification level of 1.5×. Use • The light of the Leica LED5000 CXI can be very bright. Therefore, always switch on the illuminator before you look through the eyepieces! 2. Adjust the brightness by briefly pressing the or buttons. 3.
Dimensional Drawings Leica M-series User Manual 52
134 105 90 66 87 Leica M50 Leica M50 with small incident-light base, transmitted-light base, Leica L2 illumination and 45° binocular tube 205 220 277 390 315 277 390 436 321 134 300 54 90 66 87 105 277 205 220 Leica M-series 390 User Manual 54 205 53
500 436 514 Leica M50 278 Leica M50 with large incident-light base and ErgoTube® 45° 24 116 119 301 322 301 500 436 514 255 116 119 278 24 54 301 278 255 Leica M-series User Manual 54
Leica M50 Leica M50 with small swinging-arm stand and 45° binocular tube 220 260 54 345 500 173 170 709 260 345 173 220 54 260 220 709 Leica M-series User Manual 55
330 24 324 Leica M60 Leica M60 with large incident light base and 45° binocular tube 278 116 119 301 54 66 24 324 288 301 330 255 278 116 119 54 301 278 255 66 Leica M-series User Manual 56
39 324 24 Leica M80 116 119 Leica M80 with large incident-light base and 45° binocular tube 301 278 54 255 24 324 395 288 301 66 278 116 119 54 278 301 255 66 Leica M-series User Manual 57
500 420 606 356 154 154 21 290 430 420 606 500 85 106 Leica M80 with transmitted-light base TL ST and binocular ErgoTube® 21 290 Leica M80 154 154 85 106 340 356 Leica M-series User Manual 58
Leica M80 Leica M80 with standard swinging-arm stand and 45° binocular tube 220 54 345 470 598 173 230 345 794 330 54 173 220 330 220 794 Leica M-series User Manual 59
3 Leica M80 100 42 109 331 Leica M80 with Leica LED2500 and binocular ErgoTube® 231 300 331 min. 290 max. 426 257 100 42 109 257 331 max. 426 257 231 in.
min. 290 max. 420 631 83.4 Leica M80 369 400 28.5 Leica M80 with universal stand, XL extension and binocular ErgoTube® 600 369 83.4 min. 290 max. 420 631 600 292 Leica M-series 28.
Appendix Leica M-series User Manual 62
Calculating the Total Magnification and Field of View Diameter Example Parameter MO Magnification of objective MO 1× objective ME Magnification of eyepiece ME 25×/9.5 eyepiece z Magnification changer position z Zoom position 4 q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™ q Coaxial reflected light 1.5×, tube factor r Factor 1.25× r Factor 1.
Care, Maintenance, Contact Persons We hope you enjoy using your high-performance microscope. Leica microscopes are renowned for their robustness and long service life. Observing the following care and cleaning tips will ensure that even after years and decades, your Leica microscope will continue to work as well as it did on the very first day. Warranty benefits The guarantee covers all faults in materials and manufacture. It does not, however, cover damage resulting from careless or improper handling.
Care, Maintenance, Contact Persons (cont'd.) Protection from dirt Dust and dirt will affect the quality of your results. ★★ Put a dust cover over the microscope when it will not be used for a long time. Cleaning polymer components Some components are made of polymer or are polymer-coated. They are, therefore, pleasant and convenient to handle.
