Hardware User Manual Instruction Manual

Luminex 100E User Manual xMAP Technology
4 - 6 PN 89-00002-00-013 Rev. D
4. Install the air filter.
5. Verify that the syringe works properly. See “Verify Syringe
Operation” for details below.
6. Reinstall the Luminex 100E analyzer front panel.
Verify Syringe
Operation
When you replace the syringe, you must verify that it runs properly.
If the syringe works improperly, it may not move freely or it may
leak. Leaks may occur where the metal threaded housing screws into
the syringe valve or at the bottom of the syringe.
 To verify syringe operation:
1. Turn the analyzer on.
2. Run a Prime command using LXR and observe the syringe
(found behind the front panel). See if the syringe moves freely or
if there are leaks around the syringe. If the syringe does not
move freely, contact Technical Support. If the syringe leaks,
determine from where it leaks.
If it leaks at the top of the syringe where the metal threaded
housing screws into the syringe valve, order a new syringe.
If it leaks at the bottom of the syringe cylinder, you must replace
the syringe seal. A good indicator of a leak in the syringe
cylinder is if you see saline crystals forming at the bottom of the
syringe over time.
3. When the prime completes, see if the syringe leaks sheath fluid
beneath the analyzer.
4. Repeat steps 2-4 until you complete five primes. If the syringe
moves freely and no leaks or puddles appear around the syringe,
the syringe works correctly.
5. Calibrate the analyzer to normalize settings for the analyzer
reporter channel, both classification channels, and the doublet
discriminator channel.
Use xMAP control microspheres with the analyzer to verify that
calibration and optical integrity for the system exist.