Expertise and Excellence in Electrophoresis Product Catalogue 2010/11 www.cleaverscientific.
Since its establishment in 2004, Cleaver Scientific Limited, a private familyowned company, has grown into the UK’s largest manufacturer of electrophoresis gel tanks and radiation safety equipment. By using the latest state-of-the-art injection moulding manufacturing techniques, Cleaver Scientific has developed a range of electrophoresis products acclaimed for their technical excellence in more than 80 countries worldwide.
contents multiSUB Horizontal Gel Systems page 4 multiSUB Horizontal Gel Accessories page 14 Horizontal Gel Systems - special applications page 18 omniPAGE Vertical Slab Gel Systems page 20 omniPAGE Vertical Slab - special applications page 27 Electrophoresis Equipment and Accessories page 30 Blotting page 34 Power Supplies page 38 Gel Documentation page 40 General Lab Equipment page 43 Radiation Safety page 50 see pages 16/17 for multSUB package deals see pages 32/33 for omniPAGE pack
multiSUB.... ■ Injection Moulded Construction - durable, leak-proof environment for complete safety and long life ■ Cassette Type Electrodes – inexpensive, easy to replace. Made of 99.99% corrosion resistant, pure platinum ■ Electrical Safety – lid can be located in one way only.
S E T T I N G A N E W S TA N D A R D I N HORIZONTAL ELECTROPHORESIS Corrosion resistant gold plated lid terminals Retractable connectors compatible with most major power supplies maxi midi mini The multiSUB series of Horizontal Gel Units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. All five units offer an unsurpassed combination of economy of gel and buffer volume, with gel size and sample number versatility.
multiSUB mini ■ Available with 7 x 7cm, 7 x Typical Applications 10cm or with both gel trays ■ Economic low gel and buffer volumes ■ Small lab bench footprint Designed for quick checks of low to medium numbers of samples Buffer saver blocks physically reduce the volume of a gel chamber and so reduce buffer requirements, saving cost. The multiSUB Mini is the smallest unit in the range, designed for low to medium numbers of samples.
multiSUB midi ■ Available with 10 x 7cm, 10 x 10cm or with both gel trays ■ Run up to 100 samples ■ Low buffer volumes ■ Ideal for rapid electrophoresis Typical Applications Ideal for quick checks of samples from PCR and cloning Loading guides allow easy well identification and sample loading Scoops for safe transfer of gels for Power Supplies and Product Selector see page 24/25 With gel tray options of 10 x 7cm and 10 x 10cm, the multiSUB Midi has been designed for routine horizontal gel electropho
multiSUB choice ■ Three tray options ■ Run up to 210 samples ■ Low buffer volumes ■ Multichannel pipette compatible Typical Applications combs for speed loading Ideal for restriction fragment analysis, sample prep or checking of high numbers of samples The multiSUB Choice offers a wide degree of versatility. Three tray options are available – 15 x 7cm, 15 x 10cm and 15 x 15cm – allowing the choice of one, two or all three gel length options at the time of purchase.
multiSUB maxi ■ Now available with 20 x 25cm, 20 x 20cm, 20 x 15cm or 20 x 10cm gel trays ■ Run up to 550 samples ■ Low buffer volumes ■ Ideal for extended separations Typical Applications Primarily designed for separating high numbers of samples from PCR or cloning The multiSUB Maxi is primarily designed for resolution of high numbers of samples such as from Cloning or PCR. The multiSUB Maxi allows ultra high-resolution separations over extended runs. Tray sizes correspond to standard blotter sizes.
multiSUB screen ■ Available with 26 x 16, 26 x 24 and 26 Typical Applications x 32cm or all three gel trays ■ Run up to 672 samples ■ Low buffer volumes ■ Multichannel pipette compatible combs for speed loading Ideal for checking very large numbers of samples or extended high resolution separations Designed for rapid screening of very large numbers of Clonal or PCR samples, the multiSUB Screen horizontal gel unit has a 672 maximum sample capacity per gel.
miniRapide ■ A convenient self contained solution for routine agarose gel electrophoresis ■ Ultra compact ■ Low buffer volumes ■ Multichannel pipette compatible combs for speed loading The miniRapide is a completely self contained system designed for quick checks of samples. Gel casting, running and analysis are all performed in the same ultra compact unit.
multiSUB midi-96 ■ Designed for loading of DNA ■ ■ ■ Typical Applications ■ samples from multi-well plates Ideal for high throughput electrophoresis Average run-time is just 15 to 30 minutes Direct microplate format for easy lane identification Multichannel pipette compatible combs for speed loading Ideal for analysis of up to 96 PCR-fragment length polymorphisms loaded from 96-well microplates or thermal cycler blocks The multiSUB Midi 96 Gel System is ideal for high throughput electrophoresis of PCR
multiSUB-4 ■ Separates a maximum 1200 ■ ■ ■ ■ samples in as little as 15 minutes in 4 stacked horizontal gel trays Multichannel-compatible combs and gel plate configurations compatible with microplates and thermal cycler blocks ensure rapid loading of DNA minipreps and PCR products by 8-channel pipette Double-sided 1.
accessories gel levelling table buffer saver blocks The Gel Levelling Table provides a useful way of pouring gels of uniform thickness. The table features a large 32 x 26cm platform with large thumb-wheel levelling feet at each corner. A levelling bubble is supplied. Buffer saver blocks physically reduce the volume in positive and negative compartments of a gel chamber and so reduces buffer requirements, saving cost. Blocks are available for all multiSUB Horizontal Gel Units.
buffers, agarose, markers buffers for nucleic acid electrophoresis Available in powder sachets to maintain shelf life, Cleaver Scientific TBE is available for nucleic acid gel electrophoresis techniques. Each buffer sachet, which is available in packs of 10, may be reconstituted to make 1 litre of working solution. Buffers may also be supplied in 1 litre and 5 litre volumes as ready-made 50x TAE and 10x TBE solutions.
package deals horizontals Cleaver Scientific now offers a comprehensive range of Combination Packages to address any budget or horizontal electrophoresis application.
package deals horizontals Combs and gel tray options may be changed at order.
comet assay tanks ■ For Single Cell Gel Electrophoresis ■ Minimise exposure to light and reduce background DNA damage ■ High efficiency cooling for enhanced resolution Cleaver Scientific COMET assay tanks are available in four slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantitation of DNA damage in cells.
clinical electrophoresis ■ Compact High Resolution Systems for Clinical Electrophoresis ■ Can accommodate cellulose acetate strips up to 24 x 20cm ■ Complete range of cellulose acetate gels and kits ■ Range includes Densitometer, Scanner and Software Cellulose acetate electrophoresis is an important technique in clinical diagnostics. This new range of cellulose acetate products offers a complete system solution for Research and Clinical Cellulose acetate electrophoresis procedures.
omniPAGE.... ■ Injection Moulded Mini Unit – durable, leak-proof environment for complete safety and long life ■ Compatible with all major brands of precast gels ■ Rapid Gel Casting and Loading ensures trouble free gel set-up ■ Versatile – interchangeable modules for slab gel and 2-D electrophoresis as well as electroblotting using a single universal buffer tank electroblotting module SIMPLE, RAPID, LEAK-PROOF GEL CASTING....
SETTING A NEW STANDARD IN EVERTICAL ELECTROPHORESIS Corrosion resistant, 99.99% pure platinum electrodes and gold plated terminals - easily replaced, ensuring minimum repair times omniPAGE maxi, 20x20cm omniPAGE mini, 10x10cm 2-D module casting base 2-D module electroblotting module casting base OMNI - om-ni- all, everything The omniPAGE range of Vertical Gel Units combines ease of use with high resolution separations.
omniPAGE mini ■ Injection moulded construction ■ Compatible with all 8 x 10 and 10 x 10cm precast gels (WxH) ■ Rapid gel casting and loading ■ Low buffer volumes ■ Rapid set up cooling Typical Applications ■ Run up to four gels Mini SDS PAGE, Native PAGE, Gradient, Second dimension and Nucleic acid separations internal running module, clamp version Technical Specifications PLATE DIMENSIONS, GEL DIMENSIONS, (W X L) 10 X 10CM 7.5 X 8CM UNIT DIMENSIONS 19 X 13 X 15CM (W X D X H) MAX.
omniPAGE mini wide ■ Offers the capacity of two mini gels on a single gel ■ Rapid gel casting and loading ■ Optional low or high buffer volumes ■ Rapid set up cooling uses same combs as VS20 systems For combs, see page 24 The omniPAGE mini wide vertical gel unit with a gel width of 20cm effectively allows double the number of samples to be resolved as the omniPAGE mini unit.
omniPAGE maxi ■ Ideal for high resolution separations ■ Rapid gel casting and loading ■ Optional low or high buffer volumes Typical Applications ■ Rapid set up cooling Maxi SDS PAGE, Native PAGE, Gradient, Second dimension and Nucleic acid separations Technical Specifications PLATE DIMENSIONS, GEL DIMENSIONS, (W X L) 20 X 20CM 16 X 17.5CM UNIT DIMENSIONS 26 X 16 X 28CM (W X D X H) MAX. SAMPLE CAPACITY 192 SAMPLES, 48 SAMPLES PER GEL BUFFER VOLUME MIN 1200ML; MAX 5600ML COMBS AVAILABLE : NO.
modular systems ■ Economy of bench space and cost ■ Enhanced reproducibility ■ Simple to use casting ■ Rapid set up cooling components and accessories of omniPAGE Mini, CVS10CES MINI, MINI WIDE AND The omniPAGE range of Modular Vertical Gel Systems allow multiple electrophoresis techniques to be performed in the same unit. Using the same main tank and lid, three different inserts are interchangeable for slab gel, tube gel and electroblotting techniques.
omniPAGE 2D systems MINI, MINI WIDE MAXI COMPLETE 2-D SYSTEMS AND ■ Rapid set-up electrofocusing ■ Extended accessory range ■ 10 tube capacity ■ Enhanced cooling features The omniPAGE 2-D Systems include both modules required for Slab Gel and First Dimension Electrophoresis and accessories, to provide a complete Mini, Mini Wide or Maxi 2-D system. The Tube Gel Module includes a rapid release gasket for easy tube extraction. Focusing can be accomplished in as little as three hours in the Mini Unit.
isoelectric focusing system ■ For IPG strip and IEF gel applications ■ Large cooling platform area ■ Pick and place easy to position electrodes ■ Grid for accurate electrode alignment ■ Rehydration tray also available The CSL-IEF system has been designed to perform all flat bed IEF techniques, including wick-based electrophoresis with horizontal and hand-cast IEF gels, and focusing with IPG strips. The unit includes a large cooling platform which offers increased strip capacity and active gel area.
DGGE page with gradient mixer and caster ■ Maximum 96-sample Typical Applications throughput ■ Large format – 20 x 20cm glass plates ■ Use with 100ml gradient mixer to make two 1mm parallel denaturing gradient gels ■ Compatible with microplates and thermal cycler blocks Detection of single base changes and polymorphisms in genomic cloned and amplified DNA fragments The omniPAGE-DGGE denaturing gradient gel electrophoresis unit can run two 20 x 20cm polyacrylamide gels for the detection of single base cha
large format vertical Ideal for a variety of large format vertical gel applications, these units offer advanced features for enhancing gel resolution and ease of use, essential when handling gels of this size. Each unit contains ultra soft silicone seals for easy plate sealing and trouble free runs, even over extended run times. Resolution is enhanced by using an aluminium heat sink plate, essential for even sample migration.
gel dryers With a drying area of 21 x 31 cm, the Midi gel dryer can dry six 10 x 10cm gels or a single larger gel. The Maxi Gel Dryer with a 35 x 45cm drying area can dry twelve 10 x 10cm mini gels simultaneously. The unit’s microprocessor controls temperature and time, each parameter being displayed on its own LED display. The gels are heated from the base plate while the vacuum removes the moisture from below to dry the gel homogeneously.
buffers, stains, markers Fluorescent Protein Stains Cleaver Scientific is pleased to launch 3 new fluorescent stains for protein detection that are both reversible and more sensitive than many of the protein stains currently on the market. Available in blue, green and red colours as CSLblue, CSLgreen and CSLred, these protein stains can detect a minimum 1-3ng of protein per square millimetre of gel.
4-gel vertical electrophoresis packages Whether you require a package to match the market leader or one to address any budget or vertical electrophoresis technique, Cleaver Scientific now offers the ultimate range of Combination Packages that will meet your needs.
standard vertical package deals *Other Combination Packages are available; please enquire.
omniPAGE electroblotting MINI, MINI WIDE MAXI COMPLETE ELECTROBLOTTING SYSTEMS AND ■ Hinged cassettes ■ High intensity electrodes ■ Up to 4 blot capacity ■ Rapid set-up cooling The omniPAGE Electroblotting Systems include both modules required for Slab Gel Electrophoresis and Electroblotting and accessories, to provide a complete Mini, Mini Wide or Maxi gel casting, running and electroblotting system.
tank sub electroblotters ■ ■ Ideal for wet electroblotting of proteins - Western blotting Up to five gel blot cassettes utilised at any one time ■ ■ Hinged cassettes for added convenience Accommodates gel thicknesses from 0.25 up to 3mm Designed primarily for wet electroblotting of proteins, TankSub Electroblotters offer a combination of increased capacity with economy saving features.
blotting CAPILLARY BLOTTERS ■ ■ ■ Permanent wick with optimised capillary action Efficient transfer of high and low M.Wt. fragments Fast, easy set-up ■ ■ DNA transfer in 2 hours or less; RNA transfer in 3 hours or less Floating lid ensures even pressure without gel compression These Capillary Blotting systems offer a contained unit for southern and northern blotting. These units incorporate optimised permanent wicks which eliminate the requirement for overlapping wicks, offering greater consistency.
membranes, buffers, stains Cleaver Scientific now offers a range of membranes, buffers and stains for protein and nucleic acid blotting applications. Available in powder sachet form, Towbin buffer Tris/Glycine base may be reconstituted to form 1 litre of 1x working solution for wet and semi-dry transfer techniques. Simply mix 800ml of 1x working solution with 200ml methanol for complete 1x Towbin buffer. Cleaver Scientific western transfer buffer is available as a 25x concentrate.
Consort power supplies ■ Constant voltage, current or All Consort Maxi Series (EV200) power supplies have four output terminals for up to four simultaneous runs. Powerful microprocessor control allows complex programming, while manual mode permits the setting of voltage, current, power and time for routine electrophoretic runs. The parameters may also be changed temporarily without interrupting the run.
omniPAC power supplies omniPAC MAXIeasy to ■ High performance, omniPAC MAXI Programmable Power Supplies are high performance, easy to operate instruments, which can handle virtually any application from medium voltage electrophoresis to high current blotting. Offering both graphic and text displays, omniPAC MAXis are completely programmable with self prompting menus and multiple operation modes.
gel documentation Technical Specifications Type Effective Pixels CCD Maximum Aperture Shutter Speed Filters 5x optical / 4x digital zoom camera14.7 megapixels 1/1.7” high-density CCD f/2.8 (W) ~ f/4.5 (T) 15 ~ 1/4000s +3 Close up and EtBr; optional SYBR green Storage Media Computer Interface Video Out 2MB memory card USB 2.0 Hi Speed (mini-B jack) NTSC/PAL Darkroom: Multi-power Source Capture Inner White Light Safety Device Weight & Dimensions Voltage Rating MicroDoc ■ 14.
gel documentation UVIdoc HD2 ■ Simple touch button control via front LCD panel ■ Ideal for routine gel documentation and critical quantitative applications ■ Extreme sensitivity even for the faintest fluorescent gels ■ Saturation monitoring of live images to ensure they remain fully quantifiable ■ Complimentary image capture and analysis software The UVIdoc HD2 is a standalone gel documentation system. A 1.
uv transilluminators ■ Three wavelength options: ■ ■ ■ ■ 254/312/365 nm – two Dual wavelength models Long life filter High efficiency reflector Hi/Lo intensity switch Fast start up Technical Specifications FILTER SIZE 20 X 20 CM LIGHT SOURCE: 8W X 6 TUBES UV RESISTANT PLASTIC COVER: 33 X 25CM (WXD) UNIT DIMENSIONS WXLXH 34 X 29.
heaters and stirrers ■ Large work surface, 19 x 19 cm ■ Space saving design, 20 x 23 cm Footprint ■ Chemical resistant white ceramic top plate ■ Safety LEDs indicate when heating or stirring ■ Quick and easy adjustment of heating/mixing Cleaver Scientific hotplates, stirrers and hotplate-stirrers feature an exceptionally durable, chemically resistant, white ceramic work surface. Their space-efficient design (20 x 23 cm footprint), makes them ideal for use on crowded bench tops and inside cabinets.
vortex ■ Powerful, reliable motor with optimised counter balance ■ Circular orbit for effective vortexing at any speed ■ CombiCup head accepts a variety of tube sizes ■ Versatile head attachment accessories for microplates and different tube sizes Technical Specifications SPEED RANGE 115V 230V 0 - 3400 RPM 0 - 2850 RPM OPERATING MODES TOUCH OR CONTINUOUS AMBIENT OPERATING RANGE 4 - 65°C DIMENSIONS (WXDXH) 14CM X 16CM X 13CM WEIGHT 2.
cube dry baths Cleaver Scientific’s Cube digital dry baths are available in single and dual block models, and comprise a comprehensive range of interchangeable blocks. Each digital dry bath is compact, easy-to-use and excellent value for money.
extreme temperature dry baths Cleaver Scientific’s Extreme Temperature dry baths have a working temperature range of -10°C to 100°C, making them broadly suited to techniques that use the extremities of temperature, such as DNA ligation (12°C) and denaturation (96°C). Each dry bath comprises a large conspicuous 2.6” LCD screen, and has operation modes for constant temperature and time, annealing and 1 to 4 programmable steps, each with a maximum of 9 cycles.
hybridisation/incubators With 21 models available, Cleaver Scientific incubators offer the utmost in choice and flexibility to the laboratory scientist. Each incubator model comprises microprocessor control of temperature in precise 0.1°C increments as well as continuous and programmable timer function, which are easily viewed within a 2.6” LCD screen. Advanced ventilation design technology maintains temperature uniformity and accuracy within ±0.2°C.
UV sterilisation cabinets ■ Inactivates nucleic acids in ■ ■ ■ ■ 5 to 30 minutes Doors fitted with safety switches Complete with four powerful, timer controlled UV bulbs Efficient decontamination of the complete work surface Suitable for work with 32P These UV Sterilisation Cabinets provide a convenient area for setting up PCR reactions in a nucleic acid free environment thus limiting PCR reaction contamination.
coolcube ■ Maintains 0°C for four hours Technical Specifications ■ Holds both microtubes and a PCR plate TEMPERATURE: 0°C FOR UP TO 4 HOURS ■ Can be used as a PCR workstation CAPACITY: 36 X 1.5ML 96 X 0.2ML 12 X 0.2ML PCR STRIPS 1 X 96 WELL PCR PLATE The CoolCube is designed to store samples safely on the lab bench without sample degradation caused by temperature increase or fluctuation. The CoolCube maintains temperature at approximately 0°C for up to four hours.
radiation safety Available in standard 10mm beta-protecting acrylic, 12mm gamma-attenuating lead acrylic and also as duo shielding for protection against both types of emission, Cleaver Scientific’s comprehensive range of Radiation Safety Products comprises a large selection of shields, boxes, waste bins, trays, plus assorted accessories and cabinets.
radiation safety Storage Boxes, Waste Bins and Cabinet Cleaver Scientific storage boxes are manufactured with hinged lids and accommodate interchangeable inserts that hold microtubes, centrifuge tubes, scintillation vials, universals, cryotubes and falcon tubes. Also supplied is our range of floor-standing and benchtop bins with anti-slip feet and hinged lids. These serve as an ideal solution for short-term storage of radioactive waste or radioisotopes.
Index Horizontal Electrophoresis multiSUB mini multiSUB midi multiSUB choice multiSUB maxi multiSUB screen miniRapide & batteryGel multiSUB midi-96 multiSUB-4 horizontal accessories buffers, agarose, markers package deals horizontals comet assay tanks clinical electrophoresis page 6 page 7 page 8 page 9 page 10 page 11 page 12 page 13 page 14 page 15 page 16 page 18 page 19 ISO9001:2000 approved company Vertical Electrophoresis omniPAGE mini omniPAGE mini wide omniPAGE maxi modular systems omniPAGE 2D s
