Manual Part 2

87
time the priming bolus was initiated on the implant worksheet.
If you have removed the plastic tubing
placed on the Pump outlet, DO NOT TRY
TO REPLACE THE TUBING BACK ON
THE PUMP OUTLET. You can displace
or damage the “O” ring placed around the
outlet. To measure the stroke volume, you
need to use the procedure described in Appendix G, after
the Catheter has been attached.
3. Listen for five beeps and observe that fluid is being pumped from tub-
ing attached to the Pump outlet.
4. Attach the 18 gauge needle (from the 3 ml syringe filled with RINSE
BUFFER) to the plastic tubing from the Pump outlet.
5. Remove the 3 ml syringe and fill the needle hub.
6. Insert the pipette into the hub. A meniscus of fluid will be present in
the pipette. This should be done on a flat surface with the pipette/tub-
ing assembly horizontal. (see Figure 8).
Figure 8: Testing Pump Stroke Volume with a Pipette
7. Note the exact location of the fluid meniscus in the pipette.
8. Measure the stroke volume by noting the volume displaced by a mini-
mum of 10 pulses. Record the total volume delivered and the number
of pulses on the Implant Worksheet. Divide the total volume deliv-
ered by the total number of pulses used to deliver that volume. The
volume per stroke must be between 0.40 and 0.60 µL. Record this
value on the Implant Worksheet.
!
WARNING
Dmp9196021-011_c.book Page 87 Wednesday, April 3, 2002 5:04 PM