Product Info Part 4

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Syringe 4: Rinse Buffer degassed)
6. Prime the needle. Close the stopcock.
7. Enter the Pump with the refill needle and syringe.
8. Open the stopcock and allow the Pump to fill completely with RINSE
BUFFER. When the fluid level stops moving, the Pump is filled.
9. Close the stopcock. Remove the syringe.
10. Prepare syringe 4 for aspiration. Obtain a vacuum by pulling back on
the plunger until it locks. Press the lock into the plunger groove to be
sure it is firmly secured.
11. Re-enter the Pump.
12. Open the stopcock and remove the RINSE BUFFER. After the fluid
level stops moving, wait an additional 30 seconds for all the RINSE
BUFFER and air to be removed.
13. When the time has elapsed, close the stopcock and remove the syringe
and set aside.
Syringe 5: Rinse Buffer (degassed)
14. Prime the needle. Close the stopcock.
15. Enter the Pump fill port with the refill needle.
16. Open the stopcock and allow the Pump to fill completely with RINSE
BUFFER. When the fluid level stops moving, the Pump is filled.
When the pump is filled, approximately 10 ml of RINSE BUFFER
will remain in the syringe. Leave the RINSE BUFFER in the syringe
and leave the syringe in place for the next step
.
Dmp9196021-011_c.book Page 158 Wednesday, April 3, 2002 5:07 PM