Product Info Part 4

186
9. Close the stopcock and remove the syringe.
10. Prepare syringe 2 for aspiration. Obtain a vacuum by pulling back on
the plunger until it locks. Press the lock into the plunger groove to be
sure it is firmly secured.
11. Re-enter the Pump.
12. Open the stopcock and remove the RINSE BUFFER. After the fluid
level stops moving, wait an additional 30 seconds for all the RINSE
BUFFER and air to be removed.
13. When the time has elapsed, close the stopcock and remove the
syringe.
Syringe 3: Rinse Buffer
14. Prime the needle and close the stopcock.
15. Enter the Pump fill port with the refill needle.
16. Open the stopcock and allow the Pump to fill complete with RINSE
BUFFER. When the fluid level stops moving, the Pump is filled.
When the Pump is filled, approximately 10 ml of RINSE BUFFER
will remain in the syringe. Leave the RINSE BUFFER in the syringe,
and leave the syringe in place for the next step.
Equilibrate and pull rinse buffer through system
The reservoir pressure in the Pump is equilibrated with outside ambient
pressure and RINSE BUFFER is pulled through the fluid system. This
procedure uses the following volumes in syringes 2 and 3:
• Syringe 2 with RINSE BUFFER. Expel all RINSE BUFFER
except 10 ml. Change the refill needle if desired.
• Syringe 3 with residual RINSE BUFFER from the previous sec-
tion.
Dmp9196021-011_c.book Page 186 Wednesday, April 3, 2002 5:07 PM