IC Metrodata for Win95 6.6027.153 METROHM Ltd. CH-9101 Herisau Switzerland Phone ++41 71 353 85 85 Fax ++41 71 353 89 01 8.714.
CH-9101 Herisau/Switzerland Phone ++41 71 353 85 85 Fax ++41 71 353 89 01 CompuServe 100031,3703 Internet http://www.metrohm.com E-Mail sales@metrohm.ch 6.6027.153 IC Metrodata for Win95 Version 1.44 Help! This Short Operating Guide has been kept very short. You will find only a brief introduction to the operation in section 3. For all details, please refer to the on-line Help in the program. This will provide you with the necessary information anywhere in the program quickly and conveniently. 8.714.
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Table of contents Table of contents 1 Introduction .................................................................. 1 1.1 General information .........................................................1 1.2 System requirements.......................................................1 2 Installation ..................................................................... 2 2.1 Hardware installation.......................................................2 2.2 Software installation ................................
II IC Metrodata for Win95
1.1 General information 1 Introduction 1.1 General information «IC Metrodata for Win95», the new evaluation tool consisting of the 1.714.0310 PC Board, the 6.2145.010 Dongle and the 6.6027.153 Software, is a versatile integrator system for all chromatographic methods, especially ion chromatography. The evaluation software is based on Windows 95® and supports the corresponding functions such as multitasking and easy export of data to other Windows applications.
2 Installation 2 Installation Protection against static charges Electronic components are sensitive to static charging and can be destroyed by discharges. Before you touch the PC board or any of the components inside the PC, you should earth yourself and any tools you are using by touching an earthed object (e.g. housing of the instrument or a radiator) to eliminate any static charges which exist. 2.
2.1 Hardware installation Installation of 2nd PC Board (1.714.0310) 1) Switch off PC and disconnect power cable from power socket. 2) Disconnect all other cables to computer peripherals (keyboard, display, printer, etc.). 3) Change jumper settings on board 2: only jumper at position 6 must be installed. 8 6 54 4) Disassemble PC and install 1.714.0310 PC Board in a free slot (see instruction manual of the PC). 5) Reassemble PC. Installation of the 6.2145.010 Dongle 1) PC with 1 LPT port Install 6.
2 Installation Connect IC system 1 1) Connect 6.2128.140 cable to socket 1 (channel 1) of the PC board. 2) Connect the two open cable endings to the sockets "Integr. Start" on the right terminal block 38 of the 733 IC Separation Center so that the two connections "COM" and "RUN" are connected with each other. 3) Connect the black banana plug to the black socket and the red banana plug to the red socket of the analog output 11 (0…1 V) of the 732 IC Detector. 732 RUN COM 733 1 2 Kabel 6.2128.
2.2 Software installation 2.2 Software installation The installation program copies the files from the installation diskettes to the drive and directory you specify and creates the following subdirectories: Data Methods Reports Directory for data files (*.chw) Directory for method files (*.mtw) Directory for report files (without extension) and graphic files (*.wmf) Installation with Windows 95 1) Start Windows 95. Find a drive that has at least 4 MB free storage space.
2 Installation 2.3 First program start Double-click the software icon to start the program. The program window with the opening picture is opened and the Log In window appears on the screen: Do not enter any password here, just click the Log In button. The following window appears: Enter company, division and name and click the OK button. This window appears only one time after software installation.
2.4 Security system 2.4 Security system The «IC Metrodata for Win95» program has a security system based on the list of users. Every user has his unique password and one of the following access levels: Novice Master Administrator Only most commonly used parameters can be modified by the user. Recommended for routine users. Existing data files cannot be overwritten, they are saved under a new name (8th character is increased by +1).
2 Installation 2.5 Interface configuration Open the menu item Options / Interfaces for configuration of the interfaces to the IC system. 1.714.0310 PC Board The MBoard1 driver for operation of the first PC board Board1 with 2 channels is installed automatically during the software setup. If a second PC board is installed, select Board2 with the mouse and click the Load device button. Select the file MBoard2.dew for operation of second PC board with 2 channels and click the OK button. 1.714.
3.1 General remarks 3 Learning by doing 3.
3 Learning by doing 3.2 Developing a new method This section describes the procedure how to develop a new, userspecific method based on a standard method. Preparation • Insert separating column in IC system and condition it. • Prepare standards and sample. Load standard method and run • Click on or select Load method and run from the Acquire menu. • Select the file 1m732b1.mtw in the File Open window and click the OK button.
3.2 Developing a new method • Set injection valve at 733 IC Separation Center to "FILL" (manually or by program) and fill sample loop with standard. • Set injection valve at 733 IC Separation Center to "INJECT" (manually or by program). The data acquisition is started automatically. The status display shows Measure, beside it running time, analysis time and measuring signal (in mV) are displayed.
3 Learning by doing Enter components for calibration • Click on or select Calibration / Components of the Method menu. An empty table for components is displayed below the chromatogram. • Click the Add button in the components window. A first line is added to the table containing peak number, retention time and a standard name. • Replace the name Peak1 in the column Name by the component name of the first peak. The values of the other columns can be left unchanged.
3.2 Developing a new method Enter concentrations for calibration • When all components have been entered click the Concentrations>> button. • Enter the desired concentration unit (e.g. ppm) in the Concentration units field. • Click the Add button. The Add level window appears. • Enter the desired number in the Create calibration level field. After clicking OK a new column is added in the Concentrations window. • Enter the concentrations of all components in the new Level column.
3 Learning by doing Start calibration • Click the Calibrate button. The Recalibration window appears. • Select the desired Level and click on OK. • Click the OK button in the Concentrations window. Display calibration curves • Click the Graphs>> button in the Components window. • Select the desired component in the Component field for which the calibration curve should be displayed. • Close the Component window by clicking OK. • Close the Components window below the chromatogram by clicking OK.
3.2 Developing a new method Set processing options • Click on or select Method setup of the Method menu. Select the Processing tab in the Method Setup window. • The options Switch to chromatography (return to «IC Metrodata for Win95» after finishing of the chromatogram if the program runs in background), Save chromatogram (automatic saving) and Automatic Show All (automatic scaling of axes) are selected by default.
3 Learning by doing Report output to screen • Click on or select Make report of the Process menu. The Report Options window appears. • In the Template field, select the preset standard template engmetro.rtt instead of english.rtt. • Click the Report button. A new window appears on the screen which displays the results (without chromatograms). Print report • Click on or select Print of the Report menu while the report window is open.
3.2 Developing a new method A report can also be printed out without preceding screen output: • Click on or select Make report of the Process menu. • Under Report Destination, switch off Screen and switch on Printer. • Click the Report button. The standard printing window of Windows 95 is opened where printer, printing range and number of copies can be selected. After confirmation with OK the results including the chromatogram are printed out. • Close the report window.
3 Learning by doing • Open the Peak Label tab. • Select the desired peak label. The Baseline Marker option for display of peak start and peak end is enabled by default. Enable the Label always Visible option in addition, if the peak label should be always displayed even with zooming. Afterwards, close the Appearance window with OK. or select View all from the View menu or double-click • Click on on the chromatogram to return to the initial display of all peaks.
3.2 Developing a new method Change report options • Click on or select Report options of the Method menu. • Under Items to report and More items to report, click all desired elements for the report. • Select Custom in the Quantification Method field. • Click the <
3 Learning by doing Close chromatogram window • Close the chromatogram window. A window appears with the message Changes in *.chw. Modified: Calibration, Integration, Peaks, Report. Save changes? • Click the Yes button. A window appears with the message *.chw already exists. Overwrite? • Click the Yes button. A window appears with the message Method *.mtw was modified. Save changes? • Click the Yes button. The File Save As window appears. • Click the OK button. A window appears with the message c:\.....
3.2 Developing a new method 2nd numeral: 3rd+ 4th numeral: 5th…8th numeral: Code for month (1…9 = Jan. … Sept., a…c = Oct. … Dec.) Day (01 … 31) Time (hh:mm) • Close the empty chromatogram window. The message Data acquisition is active! Standby? appears. • Click the Yes button. Open recorded chromatogram • Click on or select Open of the File menu. • Select the desired file *.chw in the Chromatogram open window.
3 Learning by doing 3.3 Use of existing methods This section describes the procedure how to record, integrate and calibrate chromatograms using existing methods. The dual1sup.mtw method is used as an illustrative example. Available methods In addition to the standard methods, the Methods sub-directory contains several methods configured for the most important separating columns supplied by Metrohm. You will find detailed information concerning these columns in the Application Bulletins (AB) mentioned.
3.3 Use of existing methods Preparations • Insert separating column in IC system and condition it. • Prepare standards and sample. Load standard method and run • Click on or select Load method and run from the Acquire menu. • Select the desired method (e.g. dual1sup.mtw) in the File Open window and click the OK button. The window Start Run appears. • Enter a name as Ident to characterize the standard (max. 14 characters, e.g. "standard"). • Change the analysis time Duration if required.
3 Learning by doing • Before starting the sample determination, you have to calibrate and perhaps modify the method. Therefore close the empty chromatogram window. The message Data acquisition is active! Standby? appears. • Click the Yes button. Open the standard chromatogram • Click on or select Open from the File menu. • Select the chromatogram *.chw just recorded in the Chromatogram open window. The lower part of the window shows the information entered at the recording of the chromatogram.
3.3 Use of existing methods • If required, enter the time delay as Delay before starting peak integration at the first peak to be detected. • Click the Apply button. The Integration Parameters window remains open and the chromatogram is reintegrated. • Repeat this procedure for all other integration parameters until the result satisfies your expectations. Click Apply after each parameter changing. • Close the Integration Parameters window with OK.
3 Learning by doing • If the Peak column contains a 0 the peak must be manually allocated to a component. In this case, insert the peak number from the chromatogram into the Peak column and click the Time field with the mouse. The corresponding retention time is then inserted automatically as the new time for this component. Start calibration • Click the Concentrations>> button. • If necessary, change the concentrations of the components in the Level 1 column. • Click the Calibrate button.
3.3 Use of existing methods • Select the Level and click on the OK button. • Click the OK button in the Concentrations window. Display calibration curves • Click the Graphs>> button in the Components window. • Select the desired component in the Component field for which the calibration curve should be displayed. • Close the Component window by clicking OK. • Close the Components window below the chromatogram by clicking OK. Save chromatogram and method • Close the chromatogram window.
3 Learning by doing • Set the injection valve at the 733 IC Separation Center to "INJECT" (manually or by program). The data acquisition is started automatically. The status display shows Measure, beside it running time, analysis time and measuring signal (in mV) are displayed. As soon as the analysis time has passed, the data acquisition is stopped automatically and the chromatogram is integrated. The status display in the upper left corner shows the message Waiting again.
3.4 Multipoint calibration 3.4 Multipoint calibration This section describes the procedure how to perform a calibration with multiple standards using either an existing or an user-developed method and to save this multipoint calibration as a new method. Preparations • Insert separating column in IC system and condition it. • Select the full scale range at the 732 IC Detector so that all peaks of the standard with the highest concentration will be inside the measuring range.
3 Learning by doing Record third to xth standard chromatogram • Click on or select Passport of the Method menu. • In the General tab, enter a new name as Ident characterizing the standard (e.g. "standard #") and click OK. • Set injection valve at 733 IC Separation Center to "FILL" (manually or by program) and fill sample loop with standard. • Set injection valve at 733 IC Separation Center to "INJECT" (manually or by program).
3.4 Multipoint calibration Enter concentrations for calibration • Select Open method of the File menu. • Select the previously saved method in the File Open window and click OK. • Select Calibration / Concentrations of the Method menu. • Click the Add button and confirm the suggested number 2 for the Level in the Add level window by clicking OK. • Enter the concentrations of all components of the second standard in the new Level 2 column. • Repeat this procedure for every further standard.
3 Learning by doing Start calibration • Click on or select Open of the File menu. • Select all previously recorded standard chromatograms in the Chromatogram open window by clicking them with the mouse. • Click the To Queue button. The Set to Queue window appears. • Enter a name for the new queue and click OK. The chromatograms are loaded into the queue and a window with the list of all chromatograms added to the queue appears.
3.4 Multipoint calibration • Because the second up to the xth standard have been recorded as samples (0) the levels belonging to the standard have to be entered into the Level column. For each level enter the level numbers already defined in the Concentrations window into this column (standard 1 = Level 1, standard 2 = Level 2, etc.). • Afterwards click the ReProcess button. In the Reprocessing window, switch on the options as shown below. • Click the Start button.
3 Learning by doing Modify calibration • Select Open method of the File menu. • Select the method used for calibration in the File Open window and click OK. An empty chromatogram window appears. • Select Calibration / Graphs of the Method menu. The Component window appears in which the calibration curves and the corresponding data are displayed.
3.4 Multipoint calibration Save method • Select Save method as of the File menu. The File Save As window appears. • Click the OK button. A window appears with the message c:\..... already exists. Overwrite? • Click the OK button. Record a sample chromatogram • Click on or select Restart method of the Acquire menu. • Enter a sample name (max. 14 characters, e.g. "drinking water") as Ident in the Start Run window. • If required, enter more parameters (e.g.
3 Learning by doing 3.5 Modify several chromatograms This section describes the procedure for reintegrating, recalculating and reprinting of several already recorded chromatograms with the aid of a queue. Load chromatograms into queue • Click on or select Open of the File menu. • Select all chromatograms to be modified in the Chromatogram open window by clicking them with the mouse. • Click the To Queue button. The Set to Queue window appears. • Enter a name for the new queue and click OK.
3.5 Modify several chromatograms • Select the desired method for recalculation of all chromatograms recorded with this method in the Chromatograms acquired by method field. • If the sample chromatograms (Level = 0) should be recalculated switch on the Sample runs option. • If the standard chromatograms (Level ≥ 1) should be recalculated switch on the Calibration runs option.
3 Learning by doing 3.6 Use of sample tables This section describes how to construct sample data tables with the aid of a queue. These tables can be processed automatically and are suited for operation with an Autosampler. Create new queue • Select Queue/Batch of the File menu. • Enter a name for the new queue in the Filename field and click OK. • In the File open window, select the method to be used in the queue. A window with a sample table appears, which contains a row for sample data entry.
3.6 Use of sample tables • Overwrite the fields of the second line with the data for the second sample. • Repeat this procedure until all sample data have been entered. Start queue • Click the Start button to start the processing of the sample table. The window Do you really wish to start the queue? appears. • Click the Yes button. The window for the on-line display of the chromatogram opens. The status display shows the message Waiting.
3 Learning by doing Start reprocessing • For reprocessing of the recorded chromatograms proceed according to section 3.5. Start existing queue • Select Queue/Batch of the File menu. • Select the desired queue *.que in the File open window and click OK. • Select Clear file names of the File menu to delete all files *.chw in the File column recorded at the last start of the queue. • Click the Start button or select Run the queue... to start the processing of the sample table.
3.7 Export data 3.7 Export data This section describes how to export data via clipboard or report output for further processing with other programs. Export chromatogram via clipboard • Click on or select Open of the File menu. • Select the desired chromatogram in the Chromatogram open window and click OK. The recorded chromatogram is displayed in a window. • Select the desired view of the chromatogram in the View menu or by zooming with the mouse (see section 3.2).
3 Learning by doing Export via file report • Click on or select Open of the File menu. • Select the desired chromatogram in the Chromatogram open window and click OK. The recorded chromatogram is displayed in a window. or select Make report of the Process menu. The win• Click on dow Report Options appears. • Switch on the File option under Report Destination. • Enter a name for the file to be saved in the Name field under File output options. • Reports and chromatogram plots are saved into the ...
3.7 Export data • If required, change the other report options (see section 3.2). Switch on the Chromatogram Plot option if also the chromatogram plot should be exported in addition to the report. • Click the Report button. The two files * (text file) and *.wmf (graphic file) are saved in the specified report directory with the desired file names and can be inserted from there into other programs. Export to Excel • Click on or select Open of the File menu.
3 Learning by doing • In the Chromatograms acquired by method window, select the desired method for export of all the chromatograms recorded with this method (chromatograms recorded with different methods cannot be exported at the same time). • If the chromatograms should not be reintegrated or recalibrated, switch off the Reintegrate and Recalibrate options. • Switch on the Sample runs option for export of sample run data and Calibration runs for export of calibration run data.
3.7 Export data • Enter the directory for storage of the exported files in the Directory field. • Enter the name of the exported file *.csv in the Name field. • Select the Overwrite option (existing data in the file are overwritten) or the Append option (data are added to the file) for Mode. • Select the Windows option for Character Set. • Click on Report. The data are saved in the *.csv file. • Open the exported file *.csv in Microsoft Excel (select file type Text files *.prn; *.txt; *.csv).
3 Learning by doing 3.8 Method associated remote control This section describes the procedure how to associate a time program for the 732 IC Detector with a method. In order to do this, a COM port of the PC and the serial interface of the 732 IC Detector must have been connected by a 6.2125.060 (25 pin/25 pin) or a 6.2125.110 (25 pin/9 pin) cable. For remote control of the 709 IC Pump, the pump must have been connected to the 732 IC Detector by a 6.2125.060 cable (see 732/733 Instruction Manual).
3.8 Method associated remote control • First click to 732 IC Detector[ COM# ] an then click the Setup button.
3 Learning by doing • Select the IC system type in the Initial tab. With Anion and Cation temperature coefficient and polarity are set automatically, with User these values can be combined independently. • Change the parameters for 732 IC Detector, 733 IC Separation Center and 709 IC Pump to the values to be set for these instruments at the start of the method. • First open the Program tab, then open the Text tab. Click the Add button to add a new program line. • Enter the time x.
3.8 Method associated remote control Example for remote instructions and time program In the Remote configuration tab, three instructions for control of an external valve with contact closure at the remote lines 3 and 4 are defined. These instructions are then used in the Text tab in the time program.
3 Learning by doing Start cycle program and data acquisition • Click on or select Restart method of the Acquire menu. • Update the parameters in the Start Run window and start method with OK. • The parameters stored in the method and the time program are sent to the 732 IC Detector. * in the lower left part of the window shows that the PC is sending data. • As soon as the program at the 732 IC Detector is ready, it will be started automatically.
3.8 Method associated remote control Start inject programm and data acquisition • Select Equipment of the Method menu. • Click on 732 IC Detector[ COM# ] in the Start with method field. Afterwards, click the Ð button to move this entry into the lower Start with inject field. • Click on or select Restart method of the Acquire menu. • Update the parameters in the Start Run window. Open the Measure tab and select the Extern option for Start mode. Start the method with OK.
3 Learning by doing togram. Therefore, program and parameters can be changed during a running method, but the changes are not effective until the next data acquisition. Stop a running program During the program run (green LED in the key of the 732 IC Detector is blinking) the key is blocked. A running program can be stopped as follows: • Stop data acquisition, reject chromatogram, stop and restart program Click on acquisition.
3.9 Manual remote control 3.9 Manual remote control This section describes the procedure how to send parameters and time programs to the 732 IC Detector and trigger manually certain functions. In order to do this, a COM port of the PC and the serial interface of the 732 IC Detector must have been connected by a 6.2125.060 (25 pin/25 pin) or a 6.2125.110 (25 pin/9 pin) cable. For remote control of the 709 IC Pump, the pump must have been connected to the 732 IC Detector by a 6.2125.
3 Bedienungslehrgang • Change the parameters for 732 IC Detector, 733 IC Separation Center and 709 IC Pump on the right side to the desired values to be set for these instruments. • Click on < . The parameters are now sent to the 732 IC Detector. * in the lower left part of the window shows that the PC is sending data.
3.9 Manual remote control Write, send and activate a program • Select Equipment setup of the Options menu. • In the Configured equipment control drivers window, click on 732 IC Detector[ COM# ] and afterwards on Setup. The INITIAL tab in the 732 IC Detector[ COM# ] window is opened. • First open the Program tab, then open the Text tab. Click the Add button to add a new program line. • Enter the time x.x in min into the first field of the program line.
3 Bedienungslehrgang • Select Equipment setup of the Options menu. • In the Configured equipment control drivers window, click on 732 IC Detector[ COM# ] and afterwards on Setup. The INITIAL tab in the 732 IC Detector[ COM# ] window is opened. • Open the Manual Control tab. • Program RUN/STOP: The program at the 732 IC Detector is started or stopped. • Valve A FILL/INJECT: Valve A at the 733 IC Separation Center is switched to the Fill or Inject position.
4.1 Software license 4 Appendix 4.1 Software license The «IC Metrodata for Win95» program is installed as a full program package together with the 6.2145.010 Dongle. Installations without dongle are demo versions which are unable to acquire chromatograms. If a demo version is equipped with a dongle, it runs as a full version. Methods generated during the demonstration time are retained in full in such a case. Please send us your 8.714.
4 Appendix 58 IC Metrodata for Win95
4.4 Index 4.4 Index A Access levels.............................................7 Analysis time ......................................10,23 Appendix .................................................57 Application Bulletins ................................22 Associate time program with existing method ...................................46 Autosampler ............................................38 Available methods...................................22 Axes settings ........................................
4 Appendix O On-Line display ...................10,23,29,39,40 Open queue during data acquisition.......39 Open recorded chromatogram ...............21 Open standard chromatogram ...............24 Operating system ......................................1 Operation with Autosampler....................38 Optional accessories...............................57 P Password...................................................7 PC Board ......................................2,8,22,57 Peak allocation ...............
