850 Professional IC AnCat – 2.850.3010 Manual 8.850.
Metrohm AG CH-9101 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com 850 Professional IC AnCat – 2.850.3010 Manual 8.850.8051EN 06.
Teachware Metrohm AG CH-9101 Herisau teachware@metrohm.com This documentation is protected by copyright. All rights reserved. Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above. Documentation in additional languages can be found on http://products.metrohm.com under Literature/Technical documentation.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents Table of contents 1 Introduction 1 1.1 Instrument description ......................................................... 1 1.2 Intended use ......................................................................... 4 1.3 About the documentation ................................................... 5 1.3.1 Symbols and conventions ........................................................ 5 1.4 Safety instructions .....................................................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 2.14 Injection valve ..................................................................... 41 2.14.1 Connecting the injection valve ............................................... 41 2.14.2 Mode of operation of the injection valve ............................... 42 2.14.3 Selecting the sample loop ...................................................... 43 2.15 Column thermostat ............................................................ 44 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 4.9 Sample degasser ................................................................. 83 4.9.1 Operation .............................................................................. 83 4.10 Injection valve .................................................................... 83 4.10.1 Protection .............................................................................. 83 4.11 Metrohm Suppressor Module (MSM) ............................... 83 4.11.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 6.16 Safety specification .......................................................... 106 6.17 Electromagnetic compatibility (EMC) ............................. 106 6.18 Weight ............................................................................... 106 7 Conformity and warranty 7.1 Declaration of Conformity ............................................... 107 7.2 Quality Management Principles ...................................... 108 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures Table of figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Figure 12 Figure 13 Figure 14 Figure 15 Figure 16 Figure 17 Figure 18 Figure 19 Figure 20 Figure 21 Figure 22 Figure 23 Figure 24 Figure 25 Figure 26 Figure 27 Figure 28 Figure 29 Figure 30 Figure 31 Figure 32 Figure 33 Figure 34 Figure 35 Figure 36 Figure 37 Figure 38 Figure 39 Figure 40 Figure 41 Figure 42 Figure 43 Figure 44 Figure 45 850 Profess
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction 1 Introduction 1.1 Instrument description The instrument 850 Professional IC – AnCat (2.850.3010) is one of the model versions of the Professional IC line of instruments manufactured by the Metrohm Company. The Professional IC line of instruments is distinguished by ￭ ￭ ￭ ￭ ￭ ￭ ￭ the intelligence of its components, which are able to monitor and optimize all functions and to provide documentation according to FDA requirements. its compactness. its flexibility.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.1 Instrument description 1 2 3 4 5 6 7 8 9 10 11 12 Figure 1 13 14 15 Front view of the instrument 1 Conductivity detectors See Chapter 2.18. 2 Peristaltic pump See Chapter 2.17. 3 MSM See Chapter 2.16. 4 Injection valve See Chapter 2.14. 5 Eluent degasser See Chapter 2.9. 6 High pressure pump See Chapter 2.10. 7 Purge valve See Chapter 2.10.1. 8 Injection valve See Chapter 2.14. 9 Eluent degasser See Chapter 2.9. 10 High pressure pump See Chapter 2.10.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction 13 Sample degasser See Chapter 2.13. Use optional. 15 Column thermostat See Chapter 2.15. 14 Separation columns See Chapter 2.21. The instrument contains the following components: Eluent degasser The eluent degasser removes gas bubbles and dissolved gases from the eluent. For degassing, the eluent flows into a vacuum chamber through a special fluoropolymer capillary.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.2 Intended use Metrohm Suppressor Module (MSM) The MSM is used for chemical suppression during anion analysis. It is pressure-stable, robust and resistant to solvents. Peristaltic pump The Peristaltic pump is used for pumping sample and auxiliary solutions. It can rotate in both directions.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.3 1 Introduction About the documentation Caution Please read through this documentation carefully before putting the instrument into operation. The documentation contains information and warnings which have to be followed by the user in order to ensure safe operation of the instrument. 1.3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.4 Safety instructions 1.4 Safety instructions 1.4.1 General notes on safety Warning This instrument may only be operated in accordance with the specifications in this documentation. This instrument has left the factory in a flawless state in terms of technical safety. To maintain this state and ensure non-hazardous operation of the instrument, the following instructions must be observed carefully. 1.4.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction Protection against electrostatic charges Warning Electronic components are sensitive to electrostatic charges and can be destroyed by discharges. Always pull the mains cable out of the mains connection socket before connecting or disconnecting electrical appliances on the rear panel of the instrument. 1.4.3 Tubing and capillary connections Caution Leaks in tubing and capillary connections are a safety risk. Tighten all connections well by hand.
1.4 Safety instructions ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ More details about the disposal of your old equipment can be obtained from your local authorities, from waste disposal companies or from your local dealer.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 2 Installation 2.1 About this chapter The Installation chapter contains ￭ ￭ ￭ ￭ 2.2 a list of all the steps to be carried out during the initial installation (see Chapter 2.2, page 9). a schematic overview of the flow paths (see Chapter 2.3, page 10). a description of the construction, connections and functionality of the instrument. step-by-step installation instructions. A part of these installation tasks has already been carried out before delivery.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.3 Flow diagram 5 Connect the leak sensor (see Chapter 2.6.4, page 21). 6 Connect drainage tubings (see Chapter 2.6.5, page 22). 7 Connect the eluent bottle (see Chapter 2.8.1, page 27). 8 Install sample path connections. ￭ ￭ Connect the sample degasser (if required) (see Chapter 2.13, page 40). Connect sample path connections to the injection valve (see Chapter 2.14.2, page 42). 9 Installing the MSM (see Chapter 2.16, page 47) – with associated peristaltic pump (see Chapter 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation The arrangement of the modules in the diagram corresponds to the front view of the instrument. Liquid containers (eluent bottle, sample vessel, waste container, auxiliary solutions container) and guard column (see Chapter 2.20, page 58) are not shown in the diagram. Which pressure screws, connections and couplings are used, is described in the installation chapters of the individual modules.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.3 Flow diagram Eluent Sample Sample optional Rinsing solution Regeneration solution 24 11 10 23 9 29 31 28 8 30 32 2 22 13 12 14 1 7 3 5 4 6 16 26 25 27 15 21 17 19 18 Figure 2 1 Flow diagram for a 2-channel system Eluent input Connection to the eluent bottle (see Chapter 2.8.1, page 27). 12 ￭￭￭￭￭￭￭￭ 20 2 Eluent degasser See Chapter 2.9.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 3 High pressure pump See Chapter 2.10. 4 Purge valve See Chapter 2.10.1. 5 Inline filter See Chapter 2.11. 6 Pulsation damper See Chapter 2.12. 7 Injection valve See Chapter 2.14. 8 Separation column See Chapter 2.21. When a guard column (see Chapter 2.20, page 58) is used, it is installed between injection valve and separation column. 9 MSM See Chapter 2.16. 10 Detector See Chapter 2.18. 11 Eluent output Connection to the waste container.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.4 Setting up the instrument 29 Regeneration solution input Connection to the regeneration solution bottle. 30 Regeneration solution output Connection to the waste container. 31 Rinsing solution input Connection to the rinsing solution bottle. 32 Rinsing solution output Connection to the waste container. 2.4 Setting up the instrument 2.4.1 Packaging The instrument is supplied in highly protective special packaging together with the separately packed accessories.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 4 1 Figure 3 2 3 Connection of capillaries with pressure screws 1 PEEK pressure screw 6.2744.014 Use on the injection valve. 2 Connection capillary 3 PEEK pressure screw, short 6.2744.070 For use on the high pressure pump, the purge valve, the inline filter, the pulsation damper, the guard column and the separation column. 4 PEEK pressure screw, long 6.2744.090 Usage on other components. Is not used on all instruments.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.6 Rear of the instrument Caution For the capillary connections between the injection valve (see Chapter 2.14, page 41) and detector (see Chapter 2.18, page 55), PEEK capillaries with an internal diameter of 0.25 mm must be used. These are already connected to a newly delivered instrument. PTFE capillaries (polytetrafluoroethylene) PTFE capillaries are transparent and enable visual tracing of the liquids to be pumped.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 1 1 2 3 5 4 Figure 4 Rollers and handle 1 Knurled screws For fastening the handle (4-2) and the rear panel of the detector chamber. 2 Handle 3 Knurled screws For fastening the roller holder (4-5). 4 Rollers 5 Roller holder Removing handle 1 Loosen knurled screws (4-1) and remove handle (4-2). Removing rollers Proceed as follows to remove the rollers: 1 Remove knurled screws (4-3). 2 Remove roller holder (4-5).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.6 Rear of the instrument Mounting handle as MPak holder Note When extended, the handle (5-2) can also be used for hanging up MPaks (eluent bags). 1 Move handle (5-2) upwards and screw in the knurled screws (5-1) again. 2 1 1 Figure 5 1 Handle as MPak holder Knurled screws For fastening the handle (5-2) and the rear panel of the detector chamber. 18 ￭￭￭￭￭￭￭￭ 2 Handle Extended. As holder for MPaks (eluent bag).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.6.2 2 Installation Positioning and connecting the detector 4 1 4 1 1 2 3 3 4 4 1 1 1 5 Figure 6 Detector 1 Detector 2 Removable rear panel 1 Knurled screws For fastening the removable rear panel. 2 Rear panel Removable 3 Cable feed-throughs For feeding through detector cables. 4 Capillary feed-throughs 5 Detector connection sockets Labeled with Detector 1 and Detector 2 for connecting Metrohm detectors.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.6 Rear of the instrument Note Up to two detectors can be positioned and connected. Caution The instrument must be turned off when connecting a detector. 1 Removing rear panel ￭ ￭ ￭ Unscrew knurled screws (6-1) on the rear panel. Remove handle if still fastened to the instrument. Remove rear panel (6-2). 2 Positioning the detector ￭ Put the detector through this opening and position it on the support surface intended for this purpose. Slide it right up to the front.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation ￭ Connect the detector cable to the detector connection socket Detector 1 (6-5). 5 Conntecting detector output Note The detector output capillary must be free of blockages (the measuring cell is tested to 5 MPa = 50 bar backpressure). Guide the detector output capillary into a sufficiently large waste container and fasten it there. 2.6.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.6 Rear of the instrument 1 2 Figure 7 Connection for the leak sensor on the rear of the instrument 1 Leak sensor connector socket Is labeled with "Leak Sensor". 3 Leak sensor connection cable Is firmly mounted on the rear of the instrument. 2.6.5 3 2 Leak sensor connector plug Drainage tubing Fluid that escapes in the covering plate or in the detector chamber flows through the drainage tubing into the base tray and past the leak sensor into the waste container.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 1 2 3 4 5 6 7 8 9 Figure 8 Drainage tubing 1 Drainage tubing connection For draining escaped fluid from the covering plate. 2 Drainage tubing Section of the 6.1816.020 silicon tubing. For draining escaped fluid from the covering plate. 3 Drainage tubing connection For draining escaped fluid from the detector chamber. 4 Drainage tubing Section of the 6.1816.020 silicon tubing. For draining escaped fluid from the detector chamber. 5 Y connector 6.1807.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.7 Capillary and cable feed-throughs 7 Drainage tubing Section of the 6.1816.020 silicon tubing. Guides escaped fluid into a waste container. 9 Drainage tubing connection For supplying escaped fluid through the connected drainage tubing to the leak sensor. 8 Drainage tubing connection For draining escaped fluid from the base tray through the connected drainage tubing.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 4 1 2 3 1 2 Figure 9 Capillary feed-throughs on the doors 1 Luer connectors For connecting a 6.2816.020 syringe. For manual sample feeding. 2 Capillary feed-through 3 PEEK pressure screws, short 6.2744.070 4 Door Do not feed capillaries through the Luer connectors (9-1). The capillaries are fastened with PEEK pressure screws (9-3) from inside to the Luer connector. From outside, liquid can be aspirated or injected with a syringe.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.7 Capillary and cable feed-throughs 5 6 7 8 8 Rear 6 5 Front 2 1 7 4 3 9 Figure 10 10 11 12 12 11 10 Capillary feed-throughs base tray/covering plate 1 Side panel (right) Right panel. 2 Rear of the instrument 3 Side panel (left) Left panel. 4 Front of the instrument 5 Capillary feed-through Upper. From front to right. 6 Capillary feed-through Upper. From front to back. 7 Capillary feed-through Upper. From front to back.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 2.8 Eluent 2.8.1 Connecting eluent bottle The eluent is aspirated out of the eluent bottle via the eluent aspiration tubing (11-1). The eluent aspiration tubing is connected to the eluent degasser (see Chapter 2.9, page 31). The tubing must be threaded through a suitable capillary feed-through (see Chapter 2.7, page 24) of the instrument before the other end can be equipped.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.8 Eluent 1 3 2 Figure 11 4 Installing eluent bottle attachment 1 Eluent aspiration tubing 6.1834.080 2 Tubing nipple From accessory set 6.1602.160. 3 O-ring From accessory set 6.1602.160. 4 Bottle attachment From accessory set 6.1602.160. 3 Mounting aspiration filter ￭ Insert filter holder (12-1) into the aspiration filter (12-2) and screw tight. 1 Figure 12 1 Filter holder From accessory set 6.2744.210.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 4 Installing tubing weighting and aspiration filter 1 3 2 Figure 13 4 5 Installing tubing weighting and aspiration filter 1 Eluent aspiration tubing 6.1834.080 2 Eluent bottle attachment 6.1602.160 3 Tubing weighting From accessory set 6.2744.210. 4 Clamping screw From accessory set 6.2744.210. 5 Aspiration filter 6.2821.090 With filter holder from accessory set 6.2744.210.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.8 Eluent Fasten the bottle attachment (13-2) on the eluent bottle (15-10). The aspiration filter (15-6) must rest on the base of the eluent bottle. ￭ 6 Mounting the adsorber tube Note In the case of alkaline eluents and eluents with lower buffer capacity, the eluent bottle must be equipped with a CO2 adsorber (15-4). First, place a piece of wadding (15-3), then the CO2 adsorber (15-4) in the large opening of the adsorber tube (15-2) and close with the plastic cover.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 5 Eluent 6 Aspiration filter 6.2821.090 7 Filter holder From accessory set 6.2744.210. 8 Clamping screw From accessory set 6.2744.210. 9 Tubing weighting From accessory set 6.2744.210. 10 Eluent bottle 6.1608.070 11 Bottle attachment 6.1602.160 12 SGJ clip 6.2023.020 13 Tubing nipple 14 Thread stopper 2.9 Eluent degasser Gas bubbles in the eluent lead to an unstable baseline, as high pressure pumps can transport liquids, but not gases.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.10 High pressure pump 3 Tubing flare With tubing nipple. 4 Clamping screw 5 Eluent aspiration tubing 6.1834.080 For aspirating the eluent. The clamping screw (16-4) is firmly mounted. 6 Connection capillary 6.1834.090 Connection from the eluent degasser to the high pressure pump (see Chapter 2.10, page 32). The clamping screw (16-4) is firmly mounted. 1 Caution The clamping screws (16-4) must be tightened carefully. Use the 6.2621.050 wrench for this. ￭ ￭ 2 ￭ ￭ ￭ 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 2 13 1 2 3 4 12 5 2 6 2 11 2 7 10 2 8 9 Figure 17 2 Capillary connections high pressure pump/purge valve 1 Connection capillary PEEK capillary, connects main piston and auxiliary piston. 2 PEEK pressure screw, short 6.2744.070 3 Outlet valve holder 4 Pump head 6.2824.110 5 Fastening screws For fastening the pump head. 6 Inlet valve holder 7 Pump head input capillary PEEK capillary at the input of the pump head.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.10 High pressure pump Note The eluent aspiration tubing is already installed in the newly delivered instrument. The following installation instructions need not be carried out at the time of initial installation. Connecting inlet to the high pressure pump 5 1 2 Figure 18 1 3 High pressure pump – Connect inlet Pressure screw For connecting the coupling (18-2) to the pump head input capillary (17-7). 2 Coupling 6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.10.2 2 Installation Deaerating the high pressure pump The high pressure pump will only operate perfectly if the pump head contains no more air bubbles. Therefore it must be deaerated during initial start-up and after every change of eluent. Caution The high pressure pump must not be deaerated before the initial startup (see Chapter 3.1, page 62).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.10 High pressure pump Deaerating the high pressure pump The instrument must be connected to the PC and switched on to deaerate the high pressure pump. 5 4 5 6 3 7 2 5 1 Figure 19 Deaerating the high pressure pump 1 Syringe 10 mL 6.2816.020 For aspirating the eluent. 2 Luer connector On purging needle. 3 Purging needle 6.2816.040 4 Deaerating capillary 5 PEEK pressure screws, short 6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 4 Setting the flow rate ￭ ￭ ￭ Start MagIC Net (if not yet started). Ensure that the eluent aspiration tubing is immersed sufficiently in the eluent. Let the high pressure pump run. 5 Aspirating eluent ￭ Aspirate with the syringe (19-1) until bubble-free eluent flows into the syringe. 6 Completing deaerating ￭ ￭ ￭ ￭ 2.11 Turn off high pressure pump. Close rotary knob (19-7). Remove syringe (19-1) from the Luer connector (19-2).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.12 Pulsation damper Installing the inline filter Caution Observe the flow direction marked on the filter housing for the connection of the inline filter. 1 Figure 20 2 3 2 4 Connecting inline filter 1 Connection capillary Connects the purge valve with the inline filter 2 PEEK pressure screws, short 6.2744.070 3 Inline filter 6.2821.120 Protects against particles. 4 Connection capillary Connects the inline filter with the pulsation damper.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation The pulsation damper protects the separation column from damage caused by pressure fluctuations when switching the injection valve, and reduces interfering pulsations during highly sensitive measurements. In order to ensure these functionalities, it must be connected between the high pressure pump (see Chapter 2.10, page 32) and injection valve (see Chapter 2.14, page 41). The pulsation damper can be operated in both directions.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.13 Sample degasser 2.13 Sample degasser The sample degasser removes gas bubbles and disolved gases from the sample. For degassing, the sample flows into a vacuum chamber through a special fluoropolymer capillary. Gas bubbles in the sample lead to poor reproducibility, as the quantity of sample in the sample loop would not always be the same. Samples (containing gas) should therefore be degassed before injection.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 4 Guide the other end of the connection capillary out of the instrument through a capillary feed-through. Caution If the sample degasser is not used, the input and output must be sealed with the 6.2744.220 thread stoppers. 2.14 Injection valve The injection valve connects the eluent and sample path.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.14 Injection valve 3 Connection capillary Connected to connector 4. Carries eluent to the injection valve. 4 Connection capillary (column inlet capillary) Connected to connector 5. Carries eluent to the separation column. 5 Connection capillary Connected to connector 1. Carries sample to the injection valve. 6 Connection capillary Connected to connector 2. Carries sample to the waste container. 7 PEEK pressure screw 6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 3 4 2 3 4 1 3 2 6 1 3 A 6 B 5 4 5 1 Figure 24 4 2 5 1 2 Injection valve – Positions A Position FILL B Position INJECT 1 Eluent input Capillary coming from the high pressure pump. 2 Eluent output Capillary to the column. 3 Sample input Sample aspirating capillary. 4 Sample output Capillary to waste container. 5 Sample loop 2.14.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.15 Column thermostat 2.15 Column thermostat The column thermostat controls the temperature of the column and eluent channel and thus ensures stable measuring conditions. It provides space for 2 separation columns.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 1 3 1 2 2 1 1 1 Figure 25 1 Column thermostat Capillary feed-throughs For guiding the capillaries in and out. 2 Capillary recesses For controlling the temperature of the eluent. Preheating capillary already pre-installed. 3 Column holder For fastening the columns. With column recognition. The column thermostat contains two column holders (25-3) equipped with chip recognition. The separation columns can be clicked into the column holder with the chip.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.15 Column thermostat Note The column input capillary is already threaded into the capillary recesses of the column thermostat at the time the instrument is first delivered. The following installation instructions need not be carried out at the time of initial installation. Threading capillaries 1 Introduce column input capillary into the column thermostat via a suitable capillary feed-through (25-1).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 2.16 Metrohm Suppressor Module (MSM) 2.16.1 General information on the MSM The MSM is used for chemical suppression during anion analysis. It is pressure-stable, robust and resistant to solvents. It consists of 3 suppressor units in total, which are used for suppression, regenerated with sulfuric acid or rinsed with ultra pure water in rotation.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.16 Metrohm Suppressor Module (MSM) 3 4 8 1 1 2 2 3 5 7 6 Figure 26 MSM – connections 1 Union nut 2 MSM connecting piece 6.2832.010 3 Eluent input capillary Labeled with Eluent. 4 Eluent output capillary Labeled with Detector. 5 Rinsing solution input capillary Labeled with H2O. 6 Rinsing solution output capillary Labeled with Waste. 7 Regeneration solution output capillary Labeled with Waste. 8 Regeneration solution input capillary Labeled with H2SO4.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation Connecting the capillaries of the MSM 1 Connecting the eluent input capillary ￭ Fasten the end of the input capillary labeled with Eluent with a 6.2744.070 short PEEK pressure screw to the output of the column. 2 Connecting the eluent output capillary ￭ Fasten the end of the output capillary labeled with Detector with a 6.2744.090 long PEEK pressure screw to the input of the MCS (insofar as an MCS is being used).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.17 Peristaltic pump 2.17 Peristaltic pump 2.17.1 Principle of the peristaltic pump The Peristaltic pump is used for pumping sample and auxiliary solutions. It can rotate in both directions. The peristaltic pump pumps liquids according to the principle of displacement. The pump tubing is clamped between the rollers (27-3) and the tubing cartridge (27-5).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.17.2 1 2 Installation Installing the peristaltic pump 2 3 4 5 6 7 8 1 6.2744.180 6.2744.160 9 Figure 28 10 3 6 7 8 Installing the pump tubing 1 PEEK pressure screws, short (6.2744.070) 2 Tubing olive (6.2744.034) 3 Stopper The colors of the stopper indicate the inner diameter of the pump tubing. 4 Tubing cartridge (6.2755.000) 5 Contact pressure lever 6 Union nut 7 Adapter 8 Tubing olive Either with filter holder (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.17 Peristaltic pump 3 Connecting the pressure side Note Depending on the use of the peristaltic pump, on the pressure side you can either connect: ￭ ￭ Case A: a 6.2744.180 pump tubing connection with filter (see Figure 29, page 52) or Case B: a 6.2744.160 pump tubing connection without filter (see Figure 30, page 53). For pumping the auxiliary solutions to the MSM or to the SPM, a 6.2744.180 pump tubing connection with filter must be used. Case A: 6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 1 Figure 30 1 Union nut 3 Tubing olive 2 3 Install pump tubing connection without filter 2 ￭ ￭ ￭ ￭ Adapter Slide union nut (30-1) onto the pump tubing. Select a suitable adapter (30-2) and slide it onto the pump tubing. The type of adapter depends on the pump tubing (see Table 1, page 53). Place the tubing olive (30-3) onto the pump tubing. Screw the union nut (30-1) onto the tubing olive (30-3).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.17 Peristaltic pump Pump tubing Adapter 6.1826.340 (black/black) 6.1826.360 (white/white) 6.1826.380 (gray/gray) 6.1826.390 (yellow/yellow) Set flow rate The contact pressure of the tubing cartridge must be adjusted in order to regulate the flow rate. Proceed as follows: Set the contact pressure 1 ￭ ￭ ￭ ￭ Fully loosen the contact pressure lever (28-5), i.e. press it all the way down. Switch on the peristaltic pump.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.18 2 Installation Conductivity detector The conductivity detector continuously measures the conductivity of the liquid passing through and indicates these signals in digital form (DSP – Digital Signal Processing). The conductivity detector exhibits outstanding thermal stability and thus guarantees reproducible measuring conditions. 1 3 2 Figure 31 1 IC detector 1.850.9010 3 Detector input capillary Permanently installed.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.18 Conductivity detector 1 3 2 Figure 32 1 Detector cable With mounted plug. 3 Type plate With serial number. Conductivity detector rear 2 Detector output capillary Permanently installed. Note In order to prevent unnecessary peak widening after separation, the connection between the outlet of the separation column and the inlet in the detector should be kept as short as possible.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation 3 1 2 4 Figure 33 Connection detector – MSM 1 Detector input capillary 2 MSM output capillary Labeled with Detector. 3 Coupling 6.2744.040 4 Pressure screws, short 6.2744.070 2.19 Connecting the instrument 2.19.1 Connecting the instrument to the PC Note The instrument must be turned off when connecting the PC. 1 Connecting the USB cable Connect the PC connection socket of the instrument to a USB connector of the computer via the 6.2151.020 USB cable.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.20 Guard column 1 Connecting the mains cable ￭ ￭ Plug the mains cable into the mains connection socket . Connect the mains cable to the mains supply. 2 Switching the instrument on and off Switch the instrument on and off with the mains switch . After switching on, the LED on the front of the instrument flashes while a system test is carried out and the connection to the software is established.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation Note The guard column may only be installed after the initial start-up (see Chapter 3.1, page 62) of the instrument. Until then, use the 6.2744.040 coupling instead of the guard and separation column. Connecting and rinsing the guard column 1 Connecting the guard column Caution When inserting the guard column, always ensure that it is inserted correctly corresponding to the flow direction (if indicated).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.21 Separation column 2.21 Separation column The intelligent separation column (iColumn) is the heart of the ion chromatographic analysis. It separates the different components corresponding to their interactions with the column. Metrohm analytical columns are equipped with a chip on which their technical specifications and their history (first use / setting up, operating hours, injections, ... ) are saved.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation Connecting and rinsing the separation column 1 Connecting the separation column Caution When using the columns, always ensure that these are correctly inserted corresponding to the flow direction indicated. ￭ ￭ Remove stoppers from the separation column. Connect the lower end of the separation column with a 6.2744.070 short PEEK pressure screw to the outlet capillary of the guard column (if used), or to the column input capillary .
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.1 Initial start-up 3 Start-up The chapter Start-up is divided into 2 sections: 3.1 Initial start-up The initial start-up is carried out during the initial installation. Conditioning Conditioning is carried out as a final installation step and each time after the system is started. Initial start-up The initial start-up is carried out during the initial installation. The entire system is flushed before guard column and separation column are installed.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Start-up 4 Setting the contact pressure of the peristaltic pump Note This work step needs to be performed only if a peristaltic pump is being used. ￭ If peristaltic pumps are used, set the contact pressure (see "Set flow rate", page 54). 5 Rinsing the instrument without columns ￭ Rinse the instrument (without columns) with eluent for 5 minutes. The instrument is now prepared for the installation of the columns (see Chapter 2.20, page 58). 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.2 Conditioning 2 Preparing the instrument ￭ ￭ Ensure that the column is correctly mounted in accordance with the flow direction indicated on the label (arrow must point in the direction of flow). Ensure that the eluent aspiration tubing is immersed in the eluent and that there is enough eluent in the eluent bottle. 3 Checking leak-tightness ￭ ￭ In MagIC Net, start the equilibration.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance 4 Operation and maintenance 4.1 General information 4.1.1 Care Warning The instrument housing must not be opened by untrained personnel. The instrument requires appropriate care. Excess contamination of the instrument may result in functional disruptions and a reduction in the service life of the sturdy mechanics and electronics.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.2 Capillary connections 4.1.3 Operation Caution In order to avoid disturbing temperature influences, the entire system including the eluent bottle must be protected against direct sunlight. 4.1.4 Shutting down If the instrument is shut down for a longer period, the entire IC system (without separation column) must be rinsed salt-free with methanol/ultra pure water (1:4), in order to prevent eluent salts from forming crystals which may cause subsequent damage.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.3 4 Operation and maintenance Door Caution The door is made of PMMA (polymethylmetacrylate). It must never be cleaned with abrasive media or solvents. Caution Never use the door as a handle. 4.4 Eluent 4.4.1 Production The chemicals used for the production of eluents should have a degree of purity of at least "p.a.". Only ultra pure water (resistance > 18.2 MΩ*cm) may be used for dilution (this generally applies for reagents which are used in ion chromatography).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.5 High pressure pump 4.4.2 4.4.2.1 Operation Supply bottle The supply bottle with the eluent must be connected as indicated in Chapter 2.8.1, Page 27. This is above all important for eluents with volatile solvents (e.g. acetone). Moreover, condensation must also be prevented in the eluent bottle. Drop formation can change the concentration ratio in the eluent. 4.4.2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance Caution In order to spare the pump seals, the pump should not be operated dry. Therefore ensure that the eluent supply is correctly connected and that there is enough eluent in the eluent bottle each time before turning on the pump. 4.5.2 Maintenance Caution Maintenance work on the high pressure pump may not be carried out unless the instrument is switched off.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.5 High pressure pump Cleaning/replacing the zirconium oxide piston Clean one piston after the other as follows: 1 Removing the piston cartridge from the pump head Loosen the piston cartridge with a wrench and unscrew from the pump head by hand. 1 2 Figure 34 1 Removing piston 2 Pump head Piston 2 Dismantle the piston Caution On the inside of the piston cartridge there is a taut spring than can jump out of the piston cartridge if suddenly loosing tension.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance ￭ 1 Figure 35 2 3 Remove the backup ring from the pump head and lay to the other parts. 4 5 6 7 8 Components of the piston cartridge 1 Piston cartridge screw 2 Retaining washer 3 Zirconium oxide piston with piston shaft Order number: 6.2824.070. 4 Spring retainer 5 Spring Order number: 6.2824.060. 6 Inner plastic sleeve Protects from metallic abrasion.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.5 High pressure pump parts: a tip for removing the old piston seal and a sleeve for inserting the new piston seal. 1 2 Figure 36 1 Tool for piston seal 6.2617.010 2 Pin Pin for removing the old piston seal. Sleeve Sleeve for inserting the new piston seal. Caution Screwing the 6.2617.010 special tool for the piston seal into the piston seal destroys this completely! 1 Removing the piston seal Caution Avoid touching the sealing surface in the pump head (17-4) with the tool.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance 1 2 Figure 37 1 Removing the piston seal 2 Piston seal Tool for piston seal Pin of the tool. 2 Inserting the new piston seal into the tool Insert the new piston seal tightly by hand into the recess of the sleeve of the tool for the piston seal (36-2). The sealing springs must be visible from the outside. 1 2 Figure 38 1 Tool for piston seal 6.2617.010 Sleeve for inserting the new piston seal.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.5 High pressure pump 1 2 Figure 39 Inserting the piston seal into the pump head 4 Replacing the piston cartridge Screw the assembled piston cartridge back into the pump head and tighten, first by hand, then additionally by approx. 15° with a wrench. Cleaning the inlet valve and outlet valve 1 Removing valves ￭ ￭ 74 ￭￭￭￭￭￭￭￭ Unscrew the connection capillary for the auxiliary piston (17-1) from the outlet valve holder.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance 1 2 3 4 Figure 40 Removing valves 1 Outlet valve holder 2 Outlet valve Order number: 6.2824.160 3 Inlet valve Order number: 6.2824.170 4 Inlet valve holder 2 Cleaning undissected valve Clean contaminated or blocked valves initially without dismantling them completely. ￭ ￭ Rinse the valve in eluent flow and counterflow direction using a spray bottle filled with ultra pure water, RBS solution or acetone.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.5 High pressure pump ￭ ￭ Place the valve with the seal faced downwards above the recess in the holder. Push the valve components out of the valve housing using the needle of the tool. 1 2 3 4 Figure 41 Dismantling valve 1 Needle For pushing the valve components out of the valve housing. 2 Valve 3 Holder 4 Recess For collecting the valve components. The components of the valve are collected in the recess of the holder. Note The components of the valve are very small.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance 1 Figure 42 2 3 4 5 5 6 6 7 9 8 8 9 7 10 10 Components of the inlet valve and outlet valve 1 Inlet valve 6.2824.170 2 Outlet valve 6.2824.160 3 Inlet valve housing 4 Outlet valve housing 5 Sealing ring (black) 6 Sleeve 7 Sapphire sleeve The shiny side must point to ruby ball. 8 Ruby ball 9 Ceramic holder for ruby ball 10 Seal The larger opening must point outwards.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.5 High pressure pump ￭ ￭ ￭ Place the valve housing over the stacked components and hold it tightly. By tilting the tool, the valve components slide into the valve housing. Press the seal by hand well on the valve housing. 6 Checking the flow direction Rinse the valve in the direction of the arrow on the valve housing and check wether liquid is escaping on the other end.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance Mounting the pump head Note To prevent the pump head from being positioned the wrong way, it is provided with different bore hole depths for the fastening bolts, i. e. a fastening bolt is longer than all others. The bore hole with the greatest depth must therefore be assigned to the longest bolt. If this is not the case, the pump will not function perfectly. 1 Mount the pump head on the pump again using the four fastening screws (17-5).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.6 Inline filter Changing the filter The flow must be stopped before changing the filter. 1 Removing the inline filter ￭ Unscrew the pressure screws (43-1) from the inline filter. 2 Unscrewing the filter screw ￭ Screw the filter screw (43-4) out of the filter housing (43-2) with the aid of two 6.2621.000 adjustable wrenches. 3 Inserting the filter ￭ ￭ Remove the old filter (43-3) with tweezers. Place the new filter (43-3) flat in the filter housing with tweezers (43-2).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.7 4 Operation and maintenance Inline sample preparation To protect the separation column (see Chapter 2.21, Page 60) against foreign particles which can affect the separating efficiency, we recommend that all samples undergo a microfiltration (filter 0.45 µm). The ultrafiltration cell can be used for filtration (see documentation on the IC Equipment for Ultrafiltration). Samples containing significant amounts of gas should be degassed. The sample degasser (see Chapter 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.8 Rinsing the sample path 2 Aspirating the sample and measuring time Aspirate a sample typical for the later application and use a stop watch to measure the time required by the sample to travel from the sample vessel to the end of the sample loop. The time measured corresponds to the "transfer time". The rinsing time should be at least 3 times the transfer time.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance 4.9 Sample degasser 4.9.1 Operation If you are working with sample degassing, the longer "transfer time" (see Ascertaining the transfer time, page 81) means that it is also necessary to rinse for longer (with the subsequent sample). The rinsing time should be at least 3 times the "transfer time" so as to minimize the carry-over effects. The "transfer time" itself depends on the pump capacity, total capillary volume and volume of gas removed (i.e.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.11 Metrohm Suppressor Module (MSM) In order to record every new chromatogram under comparable conditions, you should normally work with a freshly regenerated suppressor. Caution The MSM must never be switched over in a dry state, as there is a risk of jamming. If the MSM is in a dry state, the MSM must be rinsed for at least 5 minutes before it may be switched over. Caution In the case of reduced capacity or high backpressure, the MSM must be regenerated (see Chapter 4.11.3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance Note The high pressure pump can be used for regeneration. For this, remove the guard column and separation column and connect the capillary directly to the MSM (regenerate in opposite direction). ￭ Rinse the 3 suppressor units with the following solutions for about 15 minutes each: – Contamination with heavy metals: 1 mol/L H2SO4 + 0.1 mol/L oxalic acid – Contamination with organic cationic complexing agents: 0.1 mol/L H2SO4 / 0.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.11 Metrohm Suppressor Module (MSM) 1 2 3 4 5 Figure 44 MSM – Components 1 Union nut 2 MSM connecting piece 6.2832.010 3 MSM rotor A 6.2832.000 4 MSM housing 5 Slot in the MSM housing Cleaning the MSM Clean the MSM as follows: 1 Disconnecting the MSM from the IC system ￭ ￭ Switch off the instrument. Disconnect the MSM from the separation column, peristaltic pump and detector. 2 Dismantling the MSM ￭ ￭ ￭ Unscrew union nut (44-1) from the MSM housing (44-4).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance ￭ Check whether solution emerges at the MSM connecting piece (44-2). If one of the supply or discharge lines remained blocked, the SPM connecting piece (44-2) must be replaced (order number 6.2832.010). 4 Cleaning the MSM rotor A ￭ Clean sealing surface of the MSM rotor A (44-3) with ethanol using a lint-free cloth. 5 Inserting the MSM rotor A Caution An incorrectly inserted MSM rotor A (44-3) can be destroyed during start-up.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.11 Metrohm Suppressor Module (MSM) 4.11.3.3 Replacing parts of the MSM It may be necessary to replace parts of the MSM in the following cases: ￭ ￭ Loss of suppression capacity which cannot be eliminated (reduced phosphate sensitivity and/or significant rise in the baseline). Blockage of the MSM which cannot be eliminated (solutions can no longer be pumped through the suppressor).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance ￭ ￭ Insert the new MSM rotor A (44-3) in the MSM housing (44-4) in such a way that the tubing connections on the rear of MSM rotor A fit into the corresponding recesses inside the MSM housing and one of the three holes of MSM rotor A is visible from below in the slot (44-5) of the MSM housing. If the MSM rotor A (44-3) is correctly inserted, its sealing area will be approx. 4 mm within the MSM housing (44-4).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.12 Peristaltic pump Caution The 6.1826.xxx pump tubings consist of PVC or PP and therefore must not be used for rinsing with solutions containing acetone. In this case, use other pump tubings or use another pump for rinsing. 4.12.2 Maintenance 4.12.2.1 Pump tubing The pump tubing used in the peristaltic pump is a consumable whose service life is restricted.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance Order number Name Material Inner diameter Use 6.1826.330 Pump tubing LFL (orange/white), 3stopper PVC (Tygon) 0.64 mm No special applications. 6.1826.340 Pump tubing LFL (black/black), 3-stopper PVC (Tygon) 0.76 mm For the sample solution in inline dialysis. 6.1826.360 Pump tubing LFL (white/white), 3-stopper PVC (Tygon) 1.02 mm For sample transfer. 6.1826.380 Pump tubing LFL (gray/gray), 3-stopper PVC (Tygon) 1.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.13 Conductivity detector 3 Mounting filter screw ￭ Screw the filter screw (45-3) back into the tubing olive (45-1) and tighten by hand. Then additionally tighten with two 6.2621.000 adjustable wrenches. 4.13 Conductivity detector 4.13.1 Maintenance Caution The conductivity detector must not be opened! Warning When rinsing the detector, the pressure must not exceed 5 MPa.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation and maintenance special IC applications in the corresponding "Application Bulletins" or "Application Notes", which are available in the Internet at http:// www.metrohm.com in the Applications area or via the Metrohm agent responsible. 4.14.2 Protection To protect the separation column against foreign particles, which can affect the separating efficiency, we recommend that both the eluent and the samples undergo a microfiltration (filter 0.
4.15 Quality Management and validation with Metrohm 4.15 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Quality Management and validation with Metrohm Quality Management Metrohm offers you comprehensive support in implementing quality management measures for instruments and software. Further information on this can be found in the brochure «Quality Management with Metrohm» available from your local Metrohm agent. Validation Please contact your local Metrohm agent for support in validating instruments and software.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Troubleshooting 5 Troubleshooting 5.1 Problems and their solutions Problem Cause Remedy Marked drop in pressure Leak in the system. Check and seal connections (see Chapter 2.5, page 14). Marked rise in pressure 6.2821.120 inline filter blocked. Replace 6.2821.130 filter (see Chapter 4.6, page 79). MSM – blocked. ￭ Regenerate the MSM (see Chapter 4.11.3.1, page 84). Note: 6.2821.180 pump tubing connection with filter must be used (29-3). Conductivity detector blocked.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1 Problems and their solutions Problem Peak areas lower than expected Peristaltic pump – insufficient or no delivery rate Very noisy baseline Background conductivity too high 96 ￭￭￭￭￭￭￭￭ Cause Remedy Eluent – Evaporation of the organic solvent in the eluent. Check the eluent bottle attachment (see Figure 13, page 29). Sample – Leak in the sample path. Check the sample path. Sample – Blockage in the sample path. Check the sample path.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Problem 5 Troubleshooting Cause Remedy MSM – regeneration or rinsing solution flow problems. Check the flow of the regeneration and rinsing solution (see Chapter 2.16.2, page 47). Column chip contaminated. Clean contact surfaces of the column chip (with alcohol). Column chip defective. 1. Save column configuration in MagIC Net. 2. Notify Metrohm Service. Individual peaks greater than expected Sample – Carry-over of the samples from previous measurement.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1 Problems and their solutions Problem Cause Remedy Extreme spread of the peaks in the chromatogram. Splitting (dual peaks) Connections – dead volume in the system. Check the connections (see Chapter 2.5, page 14) (use PEEK capillaries with an internal diameter of 0.25 mm between the injection valve and detector). Guard column – diminished performance. ￭ Replace guard column (see Chapter 2.20, page 58). Separation column – dead volume at column head.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Problem 850 Professional IC – AnCat Cause 5 Troubleshooting Remedy ￭ Connect an FEP tubing between the IC instrument and the Extension Module and tighten both clamping screws until they seal.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Reference conditions 6 Technical specifications 6.1 Reference conditions The technical data listed in this Chapter refers to the following reference conditions: Ambient temperature +25 °C (± 3 °C) Instrument status > 40 minutes in operation (equilibrated) 6.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.5 6 Technical specifications Housing Dimensions Width 365 mm Height 642 mm Depth 380 mm Material of base tray, housing and bottle holder Polyurethane hard foam (PUR) with flame retardation for fire class V0, CFC-free, painted Operating elements Indicators LED for power display On/Off switch On the rear panel of the instrument 6.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.8 Sample degasser Pressure range Pump 0…50.0 MPa (0…500 bar) Pump head 0…35.0 MPa (0…350 bar) (applies for the standard PEEK pump head) Residual pulsation <1% Safety shutdown Function Automatic shutdown upon reaching the pressure limit values Maximum pressure limit ￭ Minimum pressure limit ￭ ￭ ￭ ￭ ￭ Gradient capacity Adjustable from 0.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.10 6 Technical specifications Column thermostat Type Peltier technique thermostat for two intelligent separation columns Adjustable temperature range 0…+ 80 °C, in increments of 0.1 °C Heating Ambient temperature +50 °C Cooling Ambient temperature –20 °C Temperature reproducibility ± 0.2 °C Stability < 0.05 °C Heating up time < 30 minutes from 20 to 50 °C Cooling time < 40 minutes from 50 to 20 °C 6.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.13 Conductivity measuring system 6.13 Conductivity measuring system Type ￭ ￭ Microprocessor-controlled Digital Signal Processing (DSP technology) Intelligent detector with 6 reference chromatograms Measuring range 0…15000 µS/cm single range Noise < 0.1 nS at 1 µS/cm Deviations from the linearity ￭ Drift < 0.2 nS/cm per hour Measuring rate 10 measurements per second for optimum results without filtering Resolution 0.0047 nS/cm Baseline Noise < 0.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.14 6 Technical specifications Mains connection Required voltage 100…240 V ± 10 % (auto-sensing) Required frequency 50…60 Hz ± 3 (auto-sensing) Power consumption ￭ Power supply unit ￭ ￭ ￭ 6.15 65 W for typical analysis application 25 W standby (detector to 40 °C) Up to 300 W maximum, electronically monitored internal fuse 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.16 Safety specification 6.16 Safety specification Design / Test ￭ ￭ ￭ ￭ 6.17 Electromagnetic compatibility (EMC) Emission ￭ ￭ ￭ ￭ ￭ Immunity ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ 6.18 EN/IEC/UL 61010-1 CSA-C22.2 No.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7 Conformity and warranty 7 Conformity and warranty 7.1 Declaration of Conformity This is to certify the conformity to the standard specifications for electrical appliances and accessories, as well as to the standard specifications for security and to system validation issued by the manufacturing company. Name of commodity 850 Professional IC The 850 Professional IC is an intelligent instrument for ion chromatography analysis.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.2 Quality Management Principles EN 61010-1 Safety requirements for electrical equipment for measurement, control and laboratory use This instrument meets the requirements of the ETL Listed Mark for the North American market. It conforms to the electrical safety standards UL 61010-1 and CSA-C22.2 No. 61010-1. This product is listed in Intertek’s Directory of Listed Products. Manufacturer Metrohm Ltd., CH-9101 Herisau/Switzerland Metrohm Ltd.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7 Conformity and warranty Software development Software development occurs in terms of the software life cycle. Tests are performed to detect programming errors and to assess the program’s functionality in a laboratory environment. Components All components used in the Metrohm instruments have to satisfy the quality standards that are defined and implemented for our products. Suppliers of components are audited by Metrohm as the need arises.
7.3 Warranty (guarantee) ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Faults arising from circumstances that are not the responsibility of Metrohm, such as improper storage or improper use, etc. are expressly excluded from the warranty.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8 Accessories 8 Accessories Caution Subject to change without notice! 8.1 Scope of delivery 2.850.3010 850 Professional IC – AnCat Order no. Description 1 1.850.3010 850 Professional IC AnCat 1 6.2122.0x0 Mains cable with C13 line socket IEC-60320-C13 Qty. Cable plug according to customer requirements. Switzerland: Type SEV 12 6.2122.020 Germany, …: Type CEE(7), VII 6.2122.040 USA, …: Type NEMA/ASA 6.2122.070 2 1.850.9010 IC Detector MF 2 6.1602.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.1 Scope of delivery Qty. 2 Order no. Description 6.1608.020 Glass bottle / 1000 mL / GL 45 Bottle for auxiliary solutions Width (mm): Height (mm): Volume (mL): 2 6.1608.070 Eluent bottle / 2 L / GL 45 Material: Height (mm): Volume (mL): 2 6.1609.000 Clear glass 262 2000 Adsorbing tube, large and bent For filling with adsorber material. Material: Height (mm): Inner diameter (mm): SGJ size: 1 6.1803.020 Glass 129 32 B-14/15 PTFE capillary 0.97 mm i.d.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 8 Accessories Order no. Description 6.1803.040 PTFE capillary 0.5 mm i.d. / 1 m Capillary for sample handling in IC. Material: PTFE Outer diameter (inches): 1/16 Inner diameter (mm): 0.5 Length (m): 1 1 6.1807.010 Y-connector for tubing 6-9 mm i.d. Connector for waste tubing 1 6.1815.010 Spiral band 0.5 m For holding together different cables or tubing. Length (m): 0.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.1 Scope of delivery Qty. 2 Order no. Description 6.1816.020 Silicone tubing 6 mm i.d., 1 m For waste tubing. Material: Outer diameter (mm): Inner diameter (mm): Length (m): 2 6.1826.320 Silicone rubber 9 6 1 Pump tubing LFL (orange/yellow), 3 stoppers For suppressor solutions, acceptor solution in Inline Dialysis and for Inline Ultrafiltration 2 6.2023.020 Clip for SGJ 14/15 Clip for SGJ 14/16 Material: 1 6.2151.020 POM Cable USB A - USB B 1.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 8 Accessories Order no. Description 6.2617.010 Tool for piston seal For removing and assembling the piston seal for all standard pump heads 2 6.2621.000 Adjustable wrench Max. opening: 20 mm. For IC instruments Length (mm): 1 6.2621.030 Length (mm): 1 6.2621.050 150 Hexagon key 4 mm 73 1/4 in. wrench For 1/4 in. screws.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.1 Scope of delivery Qty. 1 Order no. Description 6.2621.080 Capillary cutter For plastic capillaries. Used with IC instruments Length (mm): 118 1 6.2621.100 Hexagon key 3 mm Hexagon key 3 mm for IC Sample Processors Length (mm): 73 1 6.2626.000 Drain nozzle (ProfIC) Drain nozzle for Professional IC instruments, for mounting on front panel 2 6.2739.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 8 Accessories Order no. Description 6.2743.080 Stopper for overflow (ProfIC), 5 pieces For Professional IC instruments 1 6.2744.014 Pressure screw 2x With UNF 10/32 connection. For the connection of PEEK capillaries Material: PEEK Length (mm): 26 2 6.2744.020 For IC instruments Material: Length (mm): 1 6.2744.034 Luer/UNF coupling PEEK 19 Coupling nozzle - UNF 10/32, 2 pieces Connection pressure screw and pump tubing. 2 pieces.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.1 Scope of delivery Qty. 3 Order no. Description 6.2744.040 2 x UNF 10/32 coupling For connecting 1/16 in. capillaries. For IC instruments Material: PEEK Length (mm): 24 3 6.2744.070 Pressure screw short Short version. With UNF 10/32 connection. 5 pieces. For the connection of PEEK capillaries Material: PEEK Length (mm): 21 2 6.2744.090 Pressure screw long Long version. With UNF 10/32 connection. 2 pieces.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 2 8 Accessories Order no. Description 6.2744.210 Tubing adaptor for aspiration filter (ProfIC) For Professional IC instruments 2 6.2816.020 Syringe 10 mL with Luer connection For various applications in IC and VA Material: PP Length (mm): 102 Volume (mL): 10 1 6.2816.040 Purging needle With PTFE tubing and Luer connection. For syringes. For aspirating eluents. 2 6.2821.090 Aspiration filter Pore size 20 µm, set of 5 pieces. For 6.1834.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.2 Optional accessories Qty. Order no. Description 8.850.8051EN Manual for 850 Professional IC, 2.850.3010 - AnCat, English 8.2 Optional accessories 2.850.3010 850 Professional IC– AnCat Order no. Description 6.1826.310 Pump tubing LFL (orange/green) 3-stopper Pump tubing for bromate determination using the triiodide method. 6.1826.330 Pump tubing LFL (orange/white), 3-stopper For all IC instruments with peristaltic pumps.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Order no. Description 6.1826.340 Pump tubing LFL (black/black), 3-stopper 8 Accessories For the sample solution in inline dialysis 6.1826.360 Pump tubing LFL (white/white), 3-stopper For Sample Processors 6.1826.380 Pump tubing LFL (gray/gray), 3-stopper For inline dilution 6.1826.390 Pump tubing LFL (yellow/yellow), 3-stopper For the sample solution in inline filtration.
8.2 Optional accessories Order no. Description 6.2057.090 Column holder ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ For holding separation columns in Professional IC instruments. 6.2148.010 Remote Box MSB An additional remote interface for the connection of devices that are controlled via remote lines. With permanently attached cable. 6.2617.040 Tool for piston seal Macro For removing and assembling the piston seal for Macro pump heads 6.2741.040 PE/PTFE piston seal Macro For all Macro pump heads 6.2824.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Order no. Description 6.6059.003 MagIC Net™ Multi – 10 additional licenses 8 Accessories 10 additional licenses 6.6059.112 MagIC Net™ 1.1 Professional CD : 1 license Professional PC program for controlling intelligent Professional IC systems, Compact IC systems and their peripherals such as Professional Sample Processor, 800 Dosino, 771 Compact Interface, etc.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Index Numbers/Symbols 6.2821.090 aspiration filter ...... 68 6.2821.130 filter ...................... 79 A Accessories Optional ........................... 120 Ambient conditions ................ 100 Anion channel .......................... 10 Aspiration filter 6.2821.090 ...... 68 Aspiration tubing for eluent ...... 27 B Baseline Conditioning ...................... 64 Unstable ............................. 69 Blockage Conductivity detector ......... 92 Blood .................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Immunity ................................ 106 Impurity MSM Organic .............................. 84 Initial installation ........................ 9 Inject Injection valve .................... 43 Injection valve ............................ 3 Fill ...................................... 43 Inject .................................. 43 Installation ................. 41, 102 Maintenance ...................... 83 Protection .......................... 83 Inline filter ......................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Separation column Installation ......................... 60 Protection ................ 3, 39, 93 Regeneration ...................... 93 Rinsing ............................... 61 Separating efficiency .......... 92 Storage .............................. 93 Service ................................. 6, 65 Service Agreement ................... 94 Shutting down ......................... 66 Standards ............................... 106 Start-up ....................................
