881 Compact IC pro 881 Compact IC pro – Anion – MCS Manual 8.881.
Metrohm AG CH-9100 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com 881 Compact IC pro 881 Compact IC pro – Anion – MCS 2.881.0030 Manual 8.881.8014EN 05.
Teachware Metrohm AG CH-9100 Herisau teachware@metrohm.com This documentation is protected by copyright. All rights reserved. Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above. Documentation in additional languages can be found on http://products.metrohm.com under Literature/Technical documentation.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents Table of contents 1 Introduction 1 1.1 Instrument description ......................................................... 1 1.2 Intended use ......................................................................... 3 1.3 About the documentation ................................................... 3 1.3.1 Symbols and conventions ........................................................ 3 1.4 Safety instructions .....................................................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 3.12 Pulsation absorber ............................................................. 36 3.13 Sample degasser ................................................................. 37 3.14 Injection valve ..................................................................... 39 3.14.1 Connecting the injection valve ............................................... 39 3.14.2 Mode of operation of the injection valve ............................... 40 3.14.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 5.6 Inline filter ........................................................................... 80 5.6.1 Maintenance ......................................................................... 80 5.7 Sample degasser ................................................................. 82 5.7.1 Operation .............................................................................. 82 5.8 Inline sample preparation .................................................. 82 5.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 7.12 Metrohm Suppressor Module (MSM) ............................. 104 7.13 Metrohm CO2 Suppressor (MCS) ..................................... 105 7.14 Mains connection ............................................................. 105 7.15 Interfaces .......................................................................... 105 7.16 Safety specification .......................................................... 106 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures Table of figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Figure 12 Figure 13 Figure 14 Figure 15 Figure 16 Figure 17 Figure 18 Figure 19 Figure 20 Figure 21 Figure 22 Figure 23 Figure 24 Figure 25 Figure 26 Figure 27 Figure 28 Figure 29 Figure 30 Figure 31 Figure 32 Figure 33 Figure 34 Figure 35 Figure 36 Figure 37 Figure 38 Figure 39 Figure 40 Figure 41 Figure 42 881 Compact IC pro – Anion – MCS Front 8
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction 1 Introduction 1.1 Instrument description The instrument 881 Compact IC pro – Anion – MCS is one of the model versions of the 881 Compact IC pro line of instruments manufactured by the Metrohm Company. The 881 Compact IC pro line of instruments is distinguished by: ￭ ￭ ￭ ￭ ￭ ￭ the intelligence of its components, which are able to monitor and optimize all functions and to provide documentation according to FDA requirements. its compact style of construction.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.1 Instrument description of 2 µm can be replaced quickly and easily. They remove particles like e. g. bacteria and algae from the solutions. Pulsation absorber The pulsation absorber protects the separation column from damage caused by pressure fluctuations when switching the injection valve, and reduces interfering pulsations during highly sensitive measurements. Sample degasser The sample degasser removes gas bubbles and dissolved gases from the sample.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.2 1 Introduction Intended use The instrument 881 Compact IC pro – Anion – MCS is used for ion chromatographic determination of anions or polar substances with sequential suppression: ￭ ￭ Chemical suppression with the Metrohm Suppressor Module (MSM) and subsequent CO2 suppression with the Metrohm CO2 Suppressor (MCS). The use of sequential suppression reduces background conductivity to a minimum.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.4 Safety instructions Warning This symbol draws attention to a possible biological hazard. Caution This symbol draws attention to a possible damage of instruments or instrument parts. Note This symbol marks additional information and tips. 1.4 Safety instructions 1.4.1 General notes on safety Warning This instrument may only be operated in accordance with the specifications in this documentation.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction Mains voltage Warning An incorrect mains voltage can damage the instrument. Only operate this instrument with a mains voltage specified for it (see rear panel of the instrument). Protection against electrostatic charges Warning Electronic components are sensitive to electrostatic charges and can be destroyed by discharges.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.4 Safety instructions 1.4.5 Recycling and disposal This product is covered by European Directive 2002/96/EC, WEEE – Waste from Electrical and Electronic Equipment. The correct disposal of your old equipment will help to prevent negative effects on the environment and public health. More details about the disposal of your old equipment can be obtained from your local authorities, from waste disposal companies or from your local dealer.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument 2 Overview of the instrument 2.1 Front 1 9 11 12 13 10 8 2 3 7 4 Figure 1 5 6 Front 881 Compact IC pro – Anion – MCS 1 Detector chamber Room for the detector and the adsorption cartridges for the MCS. 2 Eluent degasser 3 High pressure pump 4 Purge valve 5 Inline filter 6 Pulsation absorber 7 Injection valve 8 Column heater 9 Column holder With column recognition.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.2 Rear 2.2 Rear 1 2 22 3 21 4 20 19 18 17 5 6 16 7 15 8 9 14 10 13 Figure 2 1 Drainage tubing connector For connecting the drainage tubing which leads away escaped fluids from the flask holder. 8 ￭￭￭￭￭￭￭￭ 12 11 Rear 881 Compact IC pro – Anion – MCS 2 Rear panel Removable. Access to the detector chamber.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument 3 Drainage tubing connector For connecting the drainage tubing which leads away escaped fluids from the detector chamber. 4 Transport locking screws For securing the vacuum pump when transporting the instrument. 5 Exhaust air opening For extracting the air from the vacuum chamber. Labeled with Exhaust. 6 Auxiliary connection socket For connecting a 891 Professional Analog out (2.891.0010). 7 Service connection socket For Metrohm service only.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.1 About this chapter 3 Installation 3.1 About this chapter The Installation chapter contains ￭ ￭ ￭ ￭ 3.2 this overview. a brief set of instructions for the initial installation of the 881 Compact IC pro – Anion – MCS. At each step you will find cross-references to more detailed installation instructions for individual components, should you require such aids. an installation diagram (see Chapter 3.3, page 14), showing a completely installed 881 Compact IC pro – Anion – MCS.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Connecting the eluent path ￭ ￭ ￭ ￭ Lead the eluent aspiration tubing (6.1834.080) out of the instrument through a capillary feed-through and connect it with the eluent bottle (see Chapter 3.8.1, page 25). Connect the column inlet capillary (6.1831.100) and the capillary of the MSM labeled with in to one another with a coupling (6.2744.040) and two short pressure screws (6.2744.070). Use a long pressure screw (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.2 Initial installation ￭ ￭ Connect one end of the aspirating capillary (6.1803.020) for the regeneration solution to the tubing olive on the pump tubing using a short pressure screw (6.2744.070). Guide the other end of the aspirating capillary out of the instrument through a capillary feed-through, slide it through a bottle attachment (6.1602.150) and screw the bottle attachment onto the bottle (6.1608.020) containing the regeneration solution.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation ￭ ￭ ￭ ￭ ￭ Attach the CO2 adsorption cartridge (6.2837.000) to the adsorption cartridge holder (6.2057.080) (see "Installing the adsorption cartridges", page 57). Prepare the H2O adsorption cartridge (6.2837.010) (see leaflet to the H2O adsorption cartridge) and attach it to the adsorption cartridge holder as well (see Figure 30, page 56). Plug the adapter (6.1808.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.3 Installation diagram ￭ ￭ Connect the separation column (see Chapter 3.21, page 60) – Connect the inlet oft the separation column either with the end of the column input capillary using a PEEK pressures screw (6.2744.070). or Connect the inlet of the separation column with the guard column (if used) (see leaflet to the separation column) – Connect the MSM capillary labeled with in with the output of the separation column using a PEEK pressure screw (6.2744.070).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 11 6 7 19 23 2 1 5 15 22 3 19 4 13 9 19 12 19 21 20 8 22 16 18 17 2 10 Figure 3 1 14 Installation diagram 881 Compact IC pro – Anion – MCS Eluent aspirating capillary (6.1834.080) Connected to the eluent degasser. 881 Compact IC pro – Anion – MCS 2 Column input capillary (6.1831.150) Connected to the injection valve and threaded into the capillary recesses of the column heater.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.3 Installation diagram 3 MSM eluent inlet capillary Labeled with in. 4 MSM eluent outlet capillary Labeled with out. 5 Detector inlet capillary 6 Detector outlet capillary 7 Regeneration solution aspirating capillary (6.1803.020) 8 Pump tubing (6.1826.320) With orange/yellow stoppers, for the regeneration solution. 9 MSM regeneration solution inlet capillary Labeled with regenerant. 10 MSM regeneration solution outlet capillary Labeled with waste reg..
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3.4 Setting up the instrument 3.4.1 Packaging The instrument is supplied in highly protective special packaging together with the separately packed accessories. Keep this packaging, as only this ensures safe transportation of the instrument. 3.4.2 Checks Immediately after receipt, check whether the shipment has arrived complete and without damage by comparing it with the delivery note. 3.4.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.5 Capillary connections in the IC system Pressure screws 4 1 Figure 4 2 3 Connection of capillaries with pressure screws 1 PEEK pressure screw (6.2744.014) Use on the injection valve. 2 Connection capillary 3 PEEK pressure screw, short (6.2744.070) For use on the high pressure pump, the purge valve, the inline filter, the pulsation absorber, the guard column and the separation column. 4 PEEK pressure screw, long (6.2744.090) Use on special components.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation PEEK capillaries (6.1831.030) with an internal diameter of 0.75 mm for sample handling in the ultra trace range. ￭ Caution For the capillary connections between the injection valve and detector , PEEK capillaries with an internal diameter of 0.25 mm must be used. These are already connected to a newly delivered instrument. PTFE capillaries (polytetrafluoroethylene) PTFE capillaries are transparent and enable visual tracing of the liquids to be pumped.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.6 Rear of the instrument Colored sleeves for PEEK capillaries The enclosed set of varicolored sleeves for PEEK capillaries (6.2251.000) serves to easily differentiate the various flows of liquid in the system through color coding. Each capillary leading a given liquid (e. g. eluent) can be highlighted with sleeves of the same color. To highlight a capillary, proceed as follows: 1 Slide a sleeve of a selected color over a capillary an move it to an easily visible position.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Connecting the leak sensor 1 Plug the leak sensor connector plug (5-2) into the leak sensor connection socket (5-1) on the rear of the instrument . 1 2 Figure 5 Connection for the leak sensor on the rear of the instrument 1 Leak sensor connection socket Is labeled with Leak Sensor. 3 Leak sensor connection cable Is firmly mounted on the rear of the instrument. 3.6.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.6 Rear of the instrument 1 2 3 4 5 6 7 8 9 Figure 6 Drainage tubings 1 Drainage tubing connection For draining escaped liquid from the cover. 2 Drainage tubing Section of the 6.1816.020 silicon tubing. For draining escaped liquid from the cover. 3 Drainage tubing connection For draining escaped fluid from the detector chamber. 4 Drainage tubing Section of the 6.1816.020 silicon tubing. For draining escaped fluid from the detector chamber. 5 Y connector (6.1807.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Installing drainage tubings Proceed as follows to install the drainage tubings: 1 Connect drainage tubing (6-2) to the drainage tubing connection (6-1) and shorten to the required length. 2 Connect drainage tubing (6-4) to the drainage tubing connection (6-3) and shorten to the required length. 3 Connect drainage tubing (6-2) and drainage tubing (6-4) to the Y connector (6-5).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.7 Capillary and cable feed-throughs 1 6 2 3 7 4 5 3 1 Figure 7 2 Capillary and cable feed-throughs 1 Capillary feed-through For feeding capillaries from the front to the rear of the instrument. 2 Capillary feed-through Fro feeding capillaries from the front to the right side of the instrument. 3 Capillary feed-through For feeding capillaries from the front to the left side of the instrument. 4 Luer connector For connecting a (6.2816.020) syringe.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3.8 Eluent 3.8.1 Connecting eluent bottle The eluent is aspirated out of the eluent bottle via the eluent aspiration tubing (8-1). The eluent aspiration tubing is connected to the eluent degasser (see Chapter 3.9, page 29). The tubing must be threaded through a suitable capillary feed-through of the instrument before the other end can be equipped. You will require the parts from the following accessories for equipping the eluent aspiration tubing: 6.1602.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.8 Eluent 1 3 2 Figure 8 4 Installing eluent bottle attachment 1 Eluent aspiration tubing 6.1834.080 2 Tubing nipple From accessories set 6.1602.160. 3 O-ring From accessories set 6.1602.160. 4 Bottle attachment From accessories set 6.1602.160. 3 Mounting aspiration filter ￭ Insert filter holder (9-1) into the aspiration filter (9-2) and screw tight. 1 Figure 9 1 Filter holder From accessories set 6.2744.210.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 4 Install tubing weighting and aspiration filter 1 3 2 Figure 10 4 5 Install tubing weighting and aspiration filter 1 Eluent aspiration tubing 6.1834.080 2 Eluent bottle attachment 6.1602.160 3 Tubing weighting From accessories set 6.2744.210. 4 Clamping screw From accessories set 6.2744.210. 5 Aspiration filter 6.2821.090 With filter holder from accessories set 6.2744.210.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.8 Eluent Fasten the completely equipped bottle attachment (12-10) on the eluent bottle. The aspiration filter (12-6) must rest on the base of the eluent bottle. Close the remaining small opening on the bottle attachment with a threaded stopper from the accessories set. ￭ ￭ 6 Mounting the adsorber tube Note In the case of alkaline eluents and eluents with lower buffer capacity, the eluent bottle must be equipped with a CO2 adsorber (12-4).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Wadding 4 CO2 adsorber Adsorbs CO2 from the air (e.g. Merck soda lime with indicator, no. 6839.10). 5 Eluent 6 Aspiration filter 6.2821.090 7 Filter holder From accessories set 6.2744.210. 8 Clamping screw From accessories set 6.2744.210. 9 Tubing weighting From accessories set 6.2744.210. 10 Eluent bottle 6.1608.070 11 Bottle attachment 6.1602.160 12 SGJ clip 6.2023.020 13 Tubing nipple 14 Threaded stopper 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.9 Eluent degasser Connecting the eluent degasser 1 5 3 2 4 6 Figure 13 Eluent degasser 1 Eluent degasser input 2 Eluent degasser output 3 Tubing flare With tubing nipple. 4 Clamping screw 5 Eluent aspiration tubing (6.1834.080) For aspirating the eluent. The clamping screw (13-4) is firmly mounted. 6 Connection tubing (6.1834.090) Connection from the eluent degasser to the high pressure pump (see Chapter 3.10, page 31).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.10 3 Installation High pressure pump The intelligent and low pulsation high pressure pump pumps the eluent through the system. It is equipped with a chip on which its technical specifications and "life history" (operating hours, service data, … ) are saved. The purge valve is used for deaerating (see Chapter 3.10.2, page 33) the high pressure pump. 3.10.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.10 High pressure pump 5 Fastening screws For fastening the pump head. 6 Inlet valve holder 7 Pump head inlet capillary PEEK capillary at the input of the pump head. 8 Pressure screw For connecting a PEEK capillary to the coupling (14-9). 9 Coupling For the connection of the eluent path at the input of the high pressure pump. Can be ordered together with the pressure screw (14-8) under the number (6.2744.230).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Clamping screw 5 Backup ring 3 Installation 4 Eluent aspiration tubing Eluent aspiration tubing (6.1834.080) or (6.1834.090). 1 Connecting coupling Fasten the coupling (15-2) with a pressure screw (15-1) on the pump head inlet capillary (14-7). 2 Connecting eluent aspiration tubing Caution The clamping screws must be tightened carefully. To tighten, grip the coupling (15-2) with the key (6.2739.000) and grip the clamping screw (15-3) with the wrench (6.2621.050). ￭ ￭ 3.10.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.10 High pressure pump 5 4 5 6 3 7 2 5 1 Figure 16 Deaerate the high pressure pump 1 Syringe 10 mL (6.2816.020) For aspirating the eluent. 2 Luer connector Part of the purging needle (6.2816.040) 3 Purging needle (6.2816.040) 4 Deaerating capillary 5 PEEK pressure screws, short (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 5 Aspirating eluent ￭ Aspirate with the syringe (16-1) until bubble-free eluent flows into the syringe. 6 Completing deaerating ￭ ￭ ￭ ￭ 3.11 Switch off high pressure pump. Close rotary knob (16-7). Remove syringe (16-1) from the Luer connector (16-2). Pull the purging needle (16-3) out of the deaerating capillary (16-4). Inline filter Between the purge valve and the pulsation absorber the inline filter (6.2821.120) is installed as protection against particles.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.12 Pulsation absorber 1 Figure 17 2 3 2 4 Connecting the inline filter 1 Connection capillary Connects the purge valve with the inline filter 2 PEEK pressure screws, short (6.2744.070) 3 Inline filter (6.2821.120) Protects against particles. 4 Connection capillary Connects the inline filter with the pulsation absorber. 1 Screw on the connection capillary running from the purge valve to the input side of the inline filter using a pressure screw (6.2744.070).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 6 2 5 1 3 4 Figure 18 Pulsation absorber – Connection 1 Connection capillary Connection to the inline filter. 2 Fastening screws 3 PEEK pressure screws, short (6.2744.070) 4 Holder for pulsation absorber 5 Pulsation absorber (6.2620.150) 6 Connection capillary Connection to the injection valve. 3.13 Sample degasser The sample degasser removes gas bubbles and dissolved gases from the sample.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.13 Sample degasser 1 3 4 2 Figure 19 Sample degasser 1 Sample degasser input 2 Sample degasser output 3 PEEK pressure screw, long (6.2744.090) 4 Connection capillaries (6.1803.040) Connecting the sample degasser 1 Remove and keep the threaded stoppers (6.2744.220) from the input and output of the sample degasser. 2 Connect the end of the sample aspirating capillary (6.1803.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 3 Installation Injection valve The injection valve connects the eluent and sample path. Through rapid and precise valve switchover a precise amount of sample solution defined by the size of the sample loop is injected and rinsed with eluent onto the separation column. 3.14.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 Injection valve Replacing the sample loop The sample loop can be replaced, depending on requirements. For additional information concerning selection of the appropriate sample loop, see Chapter 3.14.3, page 41. Note Use only 6.2744.010 PEEK pressure screws for connecting capillaries and sample loop to the injection valve. 1 Removing existing sample loop ￭ ￭ Loosen 6.2744.010 pressure screws at connector 3 and connector 6. Remove sample loop.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 1 Eluent input Capillary coming from the high pressure pump. 2 Eluent output Capillary to the column. 3 Sample input Sample aspirating capillary. 4 Sample output Capillary to waste container. 5 Sample loop 3.14.3 Position A In the position FILL, the sample solution flows through the sample loop to the waste container. The eluent flows directly to the separation column at the same time.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.15 Column heater 1 2 3 4 2 Figure 22 Column heater 1 Column holder For engaging the column. With column detection. 2 Capillary recesses For threading in the capillaries to be tempered 3 Holder plate For fixing the capillaries that have been threaded in. 4 Capillary feed-throughs For guiding the capillaries in and/or out of the column chamber. The column heater contains a column holder (22-1) equipped with chip recognition. The separation column (see Chapter 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation In order to bring the eluent to the required temperature, the capillaries must be guided through the capillary recesses (22-2) before connection to the separation column. Note The column input capillary is already installed in newly delivered instruments. The following installation instructions need not be carried out at the time of initial installation.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.15 Column heater 2 Retracting capillaries 3 2 5 4 5 5 1 Figure 23 Column heater – Installing capillaries 1 Column input capillary Leading from the injection valve 2 Outer capillary recess 3 Inner capillary recess 4 Holder plate 5 Screws For fastening the holder plate ￭ 44 ￭￭￭￭￭￭￭￭ Slide the column input capillary (23-1) from below into the outer of the two capillary recesses (23-2). Slide it through under the holder plate (23-4) until it emerges again at the top.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation ￭ ￭ Carefully bend the column input capillary (23-1) downward and slide it from top to bottom through the inner capillary recess (23-3) until it emerges at the lower edge of the holder plate (23-4). At the end of the column input capillary (23-1), connect the coupling 6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.16 Peristaltic pump 6 5 1 4 3 2 7 Figure 24 Peristaltic pump 1 Knurled screw in the mounting pin 2 Roller hub 3 Rollers 4 Cartridge holder 5 Tubing cartridges 6.2755.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.16.2 1 3 Installation Installing the peristaltic pump 2 3 4 5 6 7 8 1 6.2744.180 6.2744.160 9 Figure 25 10 3 6 7 8 Installing the pump tubing 1 PEEK pressure screws, short (6.2744.070) 2 Tubing olive (6.2744.034) 3 Stopper The colors of the stopper indicate the inner diameter of the pump tubing. 4 Tubing cartridge (6.2755.000) 5 Contact pressure lever 6 Union nut 7 Adapter 8 Tubing olive Either with filter holder (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.16 Peristaltic pump 3 Connecting the pressure side Note Depending on the use of the peristaltic pump, on the pressure side you can either connect: ￭ ￭ Case A: a 6.2744.180 pump tubing connection with filter (see Figure 26, page 48) or Case B: a 6.2744.160 pump tubing connection without filter (see Figure 27, page 49). For pumping the auxiliary solutions to the MSM or to the SPM, a 6.2744.180 pump tubing connection with filter must be used. Case A: 6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 1 Figure 27 1 Union nut 3 Tubing olive 2 3 Install pump tubing connection without filter 2 ￭ ￭ ￭ ￭ Adapter Slide union nut (27-1) onto the pump tubing. Select a suitable adapter (27-2) and slide it onto the pump tubing. The type of adapter depends on the pump tubing (see Table 1, page 49). Place the tubing olive (27-3) onto the pump tubing. Screw the union nut (27-1) onto the tubing olive (27-3).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.16 Peristaltic pump Pump tubing Adapter 6.1826.340 (black/black) 6.1826.360 (white/white) 6.1826.380 (gray/gray) 6.1826.390 (yellow/yellow) Set flow rate The contact pressure of the tubing cartridge must be adjusted in order to regulate the flow rate. Proceed as follows: Set the contact pressure 1 ￭ ￭ ￭ ￭ Fully loosen the contact pressure lever (25-5), i.e. press it all the way down. Switch on the peristaltic pump.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.17 3 Installation Metrohm Suppressor Module (MSM) The MSM is used for chemical suppression during anion analysis with conductivity detection or UV/VIS detection. It consists of 3 suppressor units in total, which are, in rotation, used for suppression – regenerated with 100 mmol/L sulfuric acid – rinsed with ultrapure water.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.17 Metrohm Suppressor Module (MSM) 3 4 8 1 1 2 2 3 5 7 6 Figure 28 Suppressor – connection capillaries 1 Union nut 2 Connecting piece (6.2832.010) 3 Eluent inlet capillary Labeled with in. 4 Eluent outlet capillary Labeled with out. 5 Rinsing solution inlet capillary Labeled with rinsing solution. 6 Rinsing solution outlet capillary Labeled with waste rins.. 7 Regeneration solution outlet capillary Labeled with waste reg..
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Connect the PTFE capillaries firmly mounted on the connecting piece to the other components of the IC system as follows: Connecting the capillaries of the suppressor In order to protect the Suppressor from foreign particles or bacterial growth, the following precondition must be fulfilled: Pump tubing connections with filter (6.2744.180) are installed at the pump tubing outlets of the peristaltic pump.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.18 Metrohm CO2 Suppressor (MCS) 4 Connecting the rinsing the solution outlet capillary ￭ Guide the other end of the outlet capillary labeled with waste rins. into a sufficiently large waste container and fasten it there. 5 Connecting the regeneration solution inlet capillary ￭ With a short PEEK pressure screw (6.2744.070), fasten the end of the inlet capillary labeled with regenerant to the pump tubing connection of the pump tubing which carries the regeneration solution.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 3 Installation 1 4 2 3 Figure 29 6 5 4 7 MCS – connection 1 MCS input Connection to the MSM. 2 MCS output Connection to the detector. 3 Aspirating capillary For aspirating air low in CO2 (as a result of CO2 adsorption cartridge (30-4)). 4 PEEK pressure screw, long (6.2744.090) 5 Capillary connection 6 Pressure screw, short (6.2744.070) Mounted on the air aspirating capillary. 7 Luer coupling (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.18 Metrohm CO2 Suppressor (MCS) 3.18.3 Installing the adsorption cartridges For effective CO2 removal, the air sucked through the degassing cell should be as low in CO2 as possible. To achieve this, the air is aspirated through a CO2 adsorption cartridge (6.2837.000) (30-4).. Moisture can block the CO2 adsorption cartridge. In order to prevent this, a H2O adsorption cartridge (6.2837.010) (30-7) is connected upstream.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7 3 Installation H2O adsorption cartridge (6.2837.010) For removing the H2O from the aspirated air. 8 Adsorption cartridge holder (6.2057.080) Filled with bead desiccant. 9 MCS aspirating capillary Connection to the MCS. Corresponds to (29-3). Installing the adsorption cartridges 1 Preparing the adsorption cartridge holder Push the 4 clips (30-3) into the slot of the adsorption cartridge holder (30-8).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.19 Connecting the instrument 3.19 Connecting the instrument 3.19.1 Connecting the instrument to the PC Note The instrument must be switched off when connecting the PC. 1 Connecting the USB cable Connect the PC connection socket of the instrument to a USB connector of the computer via the (6.2151.020) USB cable. 3.19.2 Connecting the instrument to mains supply Warning The power supply unit must not get wet. Protect it against the direct effect of liquids.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.20 3 Installation Guard column The use of guard columns serves for protecting the separation columns and increasing their service life considerably. The guard columns available from Metrohm represent either actual guard columns or are so-called guard column cartridges which are used together with a cartridge holder. The installation of a guard column cartridge in the associated holder is described in the leaflet of the guard columns.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.21 Separation column Connecting and rinsing the guard column 1 Connecting the guard column Caution When inserting the guard column, always ensure that it is inserted correctly corresponding to the flow direction (if indicated). ￭ ￭ ￭ Remove sealing caps and/or stoppers from the guard column. Fasten the input of the guard column to the column inlet capillary using a short PEEK pressure screw (6.2744.070).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Caution New separation columns are filled with solution and are sealed on both sides with stoppers. Before using the column, you need to ensure that this solution is miscible with the eluents used (observe manufacturer's data). You can find the separation columns and guard columns currently available from Metrohm in the Metrohm IC Column Program, or in the Internet at http://www.metrohm.com in the product area Ion Chromatography.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.21 Separation column 2 Rinsing the separation column ￭ ￭ ￭ ￭ Place beaker under the outlet end of the separation column. Set the flow rate of the high pressure pump according to the data given in the leaflet of the separation column. Start the high pressure pump and rinse the separation column approx. 10 minutes with eluent. Switch off the high pressure pump again.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Start-up 4 Start-up The chapter Start-up is divided into 2 sections: 4.1 Initial start-up The initial start-up is carried out during the initial installation. Conditioning Conditioning is carried out as a final installation step and each time after the system is started. Initial start-up The initial start-up is carried out during the initial installation. The entire system is rinsed before guard column and separation column are installed.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.2 Conditioning 4 Deaerate the high pressure pump ￭ Deaerate the high pressure pump(s) via the purge valve (see Chapter 3.10.2, page 33). 5 Set the contact pressure of the peristaltic pump Note This work step needs to be performed only if a peristaltic pump is being used. ￭ If peristaltic pumps are used, set the contact pressure (see "Set flow rate", page 50). 6 Rinsing the instrument without columns ￭ Rinse the instrument (without columns) with eluent for 5 minutes.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Start-up ￭ Select (or create) a suitable method. 2 Preparing the instrument ￭ ￭ ￭ Ensure that the column is correctly mounted according to the flow direction indicated on the label (arrow must point in the direction of flow). Ensure that the eluent aspiration tubing is immersed in the eluent and that there is enough eluent in the eluent bottle.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1 General notes 5 Operation and maintenance 5.1 General notes 5.1.1 Care Warning The instrument housing must not be opened by untrained personnel. The instrument requires appropriate care. Excess contamination of the instrument may result in functional disruptions and a reduction in the service life of the sturdy mechanics and electronics.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1.3 5 Operation and maintenance Operation Caution In order to avoid disturbing temperature influences, the entire system including the eluent bottle must be protected against direct sunlight. 5.1.4 Shutting down If the instrument is not used for a longer period, the whole IC system (except the columns) must be rinsed salt free with methanol/ultrapure water (1:4), in order to prevent eluent salts from forming crystals which may cause subsequent damage.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.3 Door 5.3 Door Caution The door is made of PMMA (polymethylmetacrylate). It must never be cleaned with abrasive media or solvents. Caution Never use the door as a handle. 5.4 Eluent 5.4.1 Production The chemicals used for the production of eluents should have a degree of purity of at least "p.a.". Only ultra pure water (resistance > 18.2 MΩ *cm) may be used for dilution (this generally applies for reagents which are used in ion chromatography).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.4.2 5.4.2.1 5 Operation and maintenance Operation Supply bottle The supply bottle with the eluent must be connected as indicated in chapter 3.8.1, page 25. This is above all important for eluents with volatile solvents (e.g. acetone). Moreover, condensation must also be prevented in the eluent bottle. Drop formation can change the concentration ratio in the eluent. 5.4.2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump Caution In order to spare the pump seals, the pump should not be operated dry. Therefore ensure that the eluent supply is correctly connected and that there is enough eluent in the eluent bottle each time before switching on the pump. 5.5.2 Maintenance Caution Maintenance work on the high pressure pump may not be carried out unless the instrument is switched off.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Cleaning/replacing the zirconium oxide piston Clean one piston after the other as follows: 1 Removing the piston cartridge from the pump head Loosen the piston cartridge with a wrench and unscrew from the pump head by hand.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump ￭ Remove the backup ring from the pump head and lay to the other parts. 1 Figure 32 2 3 4 5 6 7 8 Components of the piston cartridge 1 Piston cartridge screw 2 Retaining washer 3 Zirconium oxide piston with piston shaft Order number: 6.2824.070 4 Spring retainer 5 Spring Order number: 6.2824.060 6 Inner plastic sleeve Protects from metallic abrasion.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance for removing the old piston seal and a sleeve for inserting the new piston seal. 1 2 Figure 33 1 Tool for piston seal 2 Pin Tip for removing the old piston seal. Sleeve Sleeve for inserting the new piston seal. Caution Screwing the special tool for the piston seal (6.2617.010) into the piston seal destroys this completely! 1 Removing the piston seal Caution Avoid touching the sealing surface in the pump head (14-4) with the tool.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump 1 2 Figure 34 1 Removing the piston seal 2 Piston seal Tool for piston seal Pin of the tool. 2 Inserting the new piston seal into the tool Insert the new piston seal tightly by hand into the recess of the sleeve of the tool for the piston seal (33-2). The sealing springs must be visible from the outside. 1 2 Figure 35 1 Tool for piston seal (6.2617.010) Sleeve for inserting the new piston seal.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 1 2 Figure 36 Inserting the piston seal into the pump head 4 Replacing the piston cartridge Screw the assembled piston cartridge back into the pump head and tighten, first by hand, then additionally by approx. 15° with a wrench. Cleaning the inlet valve and outlet valve 1 Removing valves ￭ ￭ 881 Compact IC pro – Anion – MCS Unscrew the connection capillary for the auxiliary piston (14-1) from the outlet valve holder.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump 1 2 3 4 Figure 37 Removing valves 1 Outlet valve holder 2 Outlet valve Order number: 6.2824.160 3 Inlet valve Order number: 6.2824.170 4 Inlet valve holder 2 Cleaning undissected valve Clean contaminated or blocked valves initially without dismantling them completely. ￭ ￭ Rinse the valve in eluent flow and counterflow direction using a spray bottle filled with ultrapure water, RBS solution or acetone.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance ￭ ￭ Place the valve with the seal faced downwards above the recess in the holder. Push the valve components out of the valve housing using the needle of the tool. 1 2 3 4 Figure 38 Dismantling valve 1 Needle For pushing the valve components out of the valve housing. 2 valve 3 Recess For collecting the valve components. 4 Holder The components of the valve are collected in the recess of the holder.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump 1 Figure 39 2 3 4 5 5 6 6 7 9 8 8 9 7 10 10 Components of the inlet valve and outlet valve 1 Inlet valve (6.2824.170) 2 Outlet valve (6.2824.160) 3 Inlet valve housing 4 Outlet valve housing 5 Sealing ring (black) 6 Sleeve 7 Sapphire sleeve The shiny side must point to ruby ball. 8 Ruby ball 9 Ceramic holder for ruby ball 10 Seal The larger opening must point outwards.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance ￭ ￭ ￭ Place over the valve housing and hold it tightly. By tilting the tool, the valve components slide into the valve housing. Press the seal by hand well on the valve housing. 6 Checking the flow direction Rinse the valve in the direction of the arrow on the valve housing and check wether liquid is escaping on the other end. If this is not the case, the valve has to be dismantled again and be reassembled correctly (see Figure 39, page 78).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.6 Inline filter Mounting the pump head Note To prevent the pump head from being positioned the wrong way, it is provided with different bore hole depths for the fastening bolts, i. e. a fastening bolt is longer than all others. The bore hole with the greatest depth must therefore be assigned to the longest bolt. If this is not the case, the pump will not function perfectly. 1 Mount the pump head on the pump again using the four fastening screws (14-5).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Changing the filter The flow must be stopped before changing the filter. 1 Removing the inline filter ￭ Unscrew the pressure screws (40-1) from the inline filter. 2 Unscrewing the filter screw ￭ Screw the filter screw (40-4) out of the filter housing (40-2) with the aid of two adjustable wrenches (6.2621.000). 3 Inserting the filter ￭ ￭ Remove the old filter (40-3) using tweezers.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.7 Sample degasser 5.7 Sample degasser 5.7.1 Operation If you are working with sample degassing, the longer "transfer time" (see Ascertaining the transfer time, page 83) means that it is also necessary to rinse for longer (with the subsequent sample). The rinsing time should be at least 3 times the "transfer time" so as to minimize the carry-over effects. The "transfer time" itself depends on the pump capacity, total capillary volume and volume of gas removed (i.e.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.9 5 Operation and maintenance Rinsing the sample path Before a new sample can be measured, the sample path must be rinsed with it so that the measuring result is not falsified by the previous sample (Sample carry-over). In the case of automated sample feeding, the rinsing time should be at least 3 times the transfer time. The transfer time is the time required by the sample to flow from the sample vessel to the end of the sample loop.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.10 Injection valve 3 Determining "Sample B" Let "Sample B" pass through the sample path for the duration of the rinsing time, then inject and measure. 4 Calculating the sample carry-over The degree of the sample carry-over corresponds to the ratio of the peak areas of the measurement for sample B to the measurement for sample A. The lower the ratio, the lower the sample carry-over.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.11.2 5 Operation and maintenance Maintenance 5.11.2.1 Pump tubing The pump tubing used in the peristaltic pump is a consumable whose service life is restricted. The LFL pump tubing with 3 stoppers is stretched in the tubing cartridge in such a way that it comes to rest between two stoppers. This results in two possible positions for the tubing cartridge.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.11 Peristaltic pump Order number Name Material Inner diameter Use 6.1826.360 Pump tubing LFL (white/white), 3-stopper PVC (Tygon) 1.02 mm For sample transfer. 6.1826.380 Pump tubing LFL (gray/gray), 3-stopper PVC (Tygon) 1.25 mm For inline sample dilution. 6.1826.390 Pump tubing LFL (yellow/yellow), 3-stopper PVC (Tygon) 1.37 mm For the sample solution in inline ultrafiltration. 5.11.2.2 Pump tubing connection with filter The 6.2821.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 5.12 Metrohm Suppressor Module (MSM) 5.12.1 Protection To protect the suppressor against foreign particles or bacterial growth, a pump tubing connection with filter (6.2744.180) (see Figure 26, page 48) must be mounted between the peristaltic pump (see Figure 24, page 46) and the inlet capillaries of the suppressor. 5.12.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12 Metrohm Suppressor Module (MSM) 5.12.3 5.12.3.1 Maintenance Parts of the suppressor 4 3 1 2 5 Figure 42 Parts of the suppressor 1 Union nut 2 Connecting piece (6.2832.010) 3 Rotor 4 Housing 5 Slot in the housing 5.12.3.2 Regenerating the suppressor If the suppressor units are loaded for a longer period with certain heavy metals (e.g. iron) or organic impurities, these can no longer be completely removed with the standard regeneration solution.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Regenerating the suppressor 1 Disconnecting the suppressor from the IC system ￭ Disconnect the capillaries of the suppressor labeled with regenerant and rinsing solution from the IC system. 2 Connecting the suppressor to the high pressure pump ￭ Connect the inlet capillary for the regeneration solution (labeled with regenerant) to the outlet of the high pressure pump with the aid of a coupling (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12 Metrohm Suppressor Module (MSM) 5.12.3.3 Cleaning the suppressor In the following cases, it may be necessary to clean the suppressor: ￭ ￭ ￭ Increased backpressure onto the connection tubings of the suppressor.. Blockage of the suppressor which cannot be eliminated (solutions can no longer be pumped through the suppressor). Jamming of the suppressor which cannot be eliminated (suppressor can no longer be switched over).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 5 Inserting the rotor Caution An incorrectly inserted rotor can be destroyed during start-up. ￭ ￭ Insert the rotor (42-3) into the housing (42-4) in such a way that the tubing connections on the rear of rotor fit into the corresponding recesses inside the housing and one of the three holes of the rotor is visible from below in the slot of the housing (42-5). If the rotor is correctly inserted, its sealing surface will be approx.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12 Metrohm Suppressor Module (MSM) Replacing parts of the suppressor Replace the parts of the suppressor as follows: 1 Disconnecting the suppressor from the IC system ￭ ￭ Switch off the instrument. Disconnect all capillaries of the suppressor from the IC system. 2 Dismantling the suppressor ￭ ￭ ￭ Unscrew union nut (42-1) from the housing (42-4). Pull the connecting piece (42-2) and the rotor (42-3) out of the housing.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 6 Inserting the new connecting piece ￭ ￭ Insert the connecting piece (42-2) into the housing (42-4) in such a way that the connector 1 is on top and the three pins of the connecting piece fit into the corresponding recesses on the housing. Reattach the union nut (42-1) onto the coupling and tighten by hand.. 7 Connecting and conditioning the suppressor ￭ ￭ Reconnect all capillaries of the suppressor to the IC system.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.14 Separation column 1 Allow material to dry loose (not in cartridge) at 140 °C overnight and refill. Or dispose of the old material, and fill with new material. 2 Cover the packed material with cotton. 5.14 Separation column 5.14.1 Separating efficiency Which analysis quality can be attained, depends to a great extent on the separating efficiency of the separation column used.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.14.3 5 Operation and maintenance Storage Always store the separation columns sealed and filled according to the data of the column manufacturer when not using them. 5.14.4 Regeneration Note The regeneration is considered as the last measure, and not to be carried out regularly. If the separating properties of the column have deteriorated, the column can be regenerated according to the specifications of the column manufacturer.
5.15 Quality Management and validation with Metrohm ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Note You can find information on the subjects of quality management, validation and maintenance as well as an overview of the documents currently available at www.metrohm.com/com/ under Support.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6 Troubleshooting 6 Troubleshooting 6.1 Problems and their solutions Problem Cause Remedy Marked drop in pressure Leak in the system. Check all capillary connections and seal leaks, if necessary (see Chapter 3.5, page 17). Marked rise in pressure Inline filter (6.2821.120) blocked. Replace the filter (6.2821.130) (see Chapter 5.6, page 80). Suppressor – blocked. ￭ Regenerate the suppressor (see Chapter 5.12.3.2, page 88). Note: 6.2821.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Problems and their solutions Problem Peristaltic pump – insufficient or no delivery rate Very noisy baseline Background conductivity too high 98 ￭￭￭￭￭￭￭￭ Cause Remedy Sample – blockage in the sample path. Check the sample path. Sample – sample loop not (completely) filled. Prolong the sample transfer time. Sample – gas bubbles in the sample. Use sample degasser (see Chapter 3.13, page 37). MCS – not connected. Connect the MCS.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Problem 6 Troubleshooting Cause Remedy Suppressor – regeneration or rinsing solution flow problems. Check the flow of the regeneration solution and the rinsing solution. Column chip contaminated. Clean the contact surfaces of the column chip (with alcohol). Column chip defective. 1. Save column configuration in MagIC Net™. 2. Notify Metrohm Service. Individual peaks greater than expected Sample – carry-over of the samples from previous measurement.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Problems and their solutions Problem Cause Remedy Separation column – dead volume at column head. ￭ ￭ Install the separation column in reverse flow direction (if permitted by the leaflet) and rinse into a beaker. Replace separation column (see "Connecting and rinsing the separation column", page 61). Greater rise in the baseline Suppressor – reduced capacity. Regenerate the suppressor (see Chapter 5.12.3.2, page 88).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7 Technical specifications 7 Technical specifications 7.1 Reference conditions The technical data listed in this Chapter refers to the following reference conditions: Ambient temperature +25 °C (± 3 °C) Instrument status > 40 minutes in operation (equilibrated) 7.2 Instrument IC system ￭ ￭ Metal-free IC system Compact system with modular design Material Painted polyurethane hard foam without CFCs, fire class V0 Operating pressure range ￭ Intelligent components 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.5 Housing 7.5 Housing Dimensions Width 302 mm Height 562 mm Depth 368 mm Material of base tray, housing and covering plate Polyurethane hard foam (PUR) with flame retardation for fire class UL94V0, CFC-free, coated Operating elements Indicators LED for power display On/Off switch On the rear panel of the instrument 7.6 Eluent degasser Material fluoropolymer Resistance to solvents No restriction (apart from PFC) Build-up time for the vacuum < 60 s 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7 Technical specifications Pressure range Pump 0…50.0 MPa (0…500 bar) Pump head 0…35.0 MPa (0…350 bar) (applies for the standard PEEK pump head) Residual pulsation <1% Safety shutdown Function Automatic shutdown upon reaching the pressure limit values Maximum pressure limit ￭ Minimum pressure limit ￭ ￭ ￭ ￭ ￭ 7.8 Adjustable from 0.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.10 Column heater 7.10 Column heater Type Resistance heater for the thermostatization of one integrated column with a length of up to 300 mm. Adjustable temperature range + 0…+ 80 °C, in increments of 0.1 °C Ambient temperature + 5 °C … Ambient temperature + 40 °C Heating Temperature reproducibility ± 0.2 °C Stability < 0.05 °C Heating up time < 30 minutes from 20 to 40 °C 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.13 7 Technical specifications Metrohm CO2 Suppressor (MCS) Material fluoropolymer Resistance to solvents No restriction (apart from PFC) Vacuum Working area Microprocessor-controlled / stabilized Build-up time after start < 30 s Capillary volume 400 µL Recommended flow range 0.1…1.0 mL 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.16 Safety specification 1 jack plug Leak sensor Further connections Auxiliary 1 DSUB 15-pin (female) Service 1 DSUB 15-pin (female) 7.16 Safety specification Design / Test ￭ ￭ ￭ ￭ 7.17 Emission Electromagnetic compatibility (EMC) ￭ ￭ ￭ ￭ ￭ Immunity ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ 106 ￭￭￭￭￭￭￭￭ EN/IEC 61010-1 UL 61010-1 CSA-C22.2 No.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.18 1.881.0030 7 Technical specifications Weight 22.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.1 Declaration of Conformity 8 Conformity and warranty 8.1 Declaration of Conformity This is to certify the conformity to the standard specifications for electrical appliances and accessories, as well as to the standard specifications for security and to system validation issued by the manufacturing company.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8 Conformity and warranty This instrument meets the requirements of the ETL Listed Mark for the North American market. It conforms to the electrical safety standards UL 61010-1 and CSA-C22.2 No. 61010-1. This product is listed in Intertek’s Directory of Listed Products. Manufacturer Metrohm Ltd., CH-9101 Herisau/Switzerland Metrohm Ltd.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.3 Warranty (guarantee) Software development Software development occurs in terms of the software life cycle. Tests are performed to detect programming errors and to assess the program’s functionality in a laboratory environment. Components All components used in the Metrohm instruments have to satisfy the quality standards that are defined and implemented for our products. Suppliers of components are audited by Metrohm as the need arises.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8 Conformity and warranty Faults arising from circumstances that are not the responsibility of Metrohm, such as improper storage or improper use, etc. are expressly excluded from the warranty.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery 9 Accessories Note Subject to change without notice. 9.1 Scope of delivery 2.881.0030 881 Compact IC pro – Anion – MCS Order no. Description 1 1.881.0030 881 Compact IC pro – Anion – MCS 1 6.2122.0x0 Mains cable with C13 line socket IEC-60320-C13 Qty. Cable plug according to customer requirements. Switzerland: Type SEV 12 6.2122.020 Germany, …: Type CEE(7), VII 6.2122.040 USA, …: Type NEMA/ASA 6.2122.070 2 6.1602.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 2 9 Accessories Order no. Description 6.1608.020 Glass bottle / 1000 mL / GL 45 Bottle for auxiliary solutions Width (mm): Height (mm): Volume (mL): 1 6.1608.070 Eluent bottle / 2 L / GL 45 Material: Height (mm): Volume (mL): 1 6.1609.000 Clear glass 262 2000 Adsorber tube / large and bent For filling with adsorber material. Material: Height (mm): Inner diameter (mm): SGJ size: 1 6.1803.020 Glass 129 32 B-14/15 PTFE capillary 0.97 mm i.d.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery Qty. 1 Order no. Description 6.1803.040 PTFE capillary 0.5 mm i.d. / 1 m Capillary for sample handling in IC. Material: PTFE Outer diameter (inches): 1/16 Inner diameter (mm): 0.5 Length (m): 1 1 6.1807.010 Y connector for tubing i.d. 6-9 mm Connector for waste tubings 1 6.1815.010 Spiral band / 0.5 m For holding together different cables or tubing. Length (m): 0.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 2 9 Accessories Order no. Description 6.1816.020 Silicone tubing 6 mm i.d. / 1 m For drainage tubings. Material: Outer diameter (mm): Inner diameter (mm): Length (m): 2 6.1826.320 Silicone rubber 9 6 1 Pump tubing LFL (orange/yellow) , 3-stopper For suppressor solutions, acceptor solutions for inline dialysis and for inline ultrafiltration 1 6.2023.020 Clip for SGJ 14/15 Material: 1 6.2057.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery Qty. 1 Order no. Description 6.2151.020 Cable USB A - USB B / 1.8 m USB connecting cable Length (m): 1 6.2251.000 1.8 Colored sleeves for capillaries Colored pieces of heat shrink tubing for capillary coding. Three pieces each of five different colors. 1 6.2322.010 PRIMUS multi anion standard solution: Promo 1 6.2617.010 Tool for piston seal For removing and assembling the piston seal for all standard pump heads 2 6.2621.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 Order no. Description 6.2621.030 Hexagon key 4 mm Length (mm): 1 9 Accessories 6.2621.050 73 1/4 in. wrench For 1/4 in. screws. For IC instruments Length (mm): 73 1 6.2621.080 Capillary cutter For plastic capillaries.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery Qty. 1 Order no. Description 6.2621.100 Hexagon key 3 mm Hexagon key 3 mm. For IC Sample Processors Length (mm): 73 1 6.2626.000 Front drain nozzle Drain nozzle for Professional IC instruments to be mounted on the front of the instrument. 2 6.2739.000 Wrench For tightening connectors Length (mm): 1 6.2743.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 9 Accessories Order no. Description 6.2744.014 Pressure screw 2x With UNF 10/32 connection. For the connection of PEEK capillaries Material: PEEK Length (mm): 26 1 6.2744.020 For IC instruments Material: Length (mm): 1 6.2744.034 Luer/UNF coupling PEEK 19 Coupling olive/UNF 10/32 2x Connection of pressure screw and pump tubing. 2 pieces. For IC instruments with peristaltic pumps 1 6.2744.040 2 x UNF 10/32 coupling For connecting 1/16 in. capillaries.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery Qty. 2 Order no. Description 6.2744.070 Pressure screw short Short version. With UNF 10/32 connection. 5 pieces. For the connection of PEEK capillaries Material: PEEK Length (mm): 21 2 6.2744.090 Pressure screw long Long version. With UNF 10/32 connection. 2 pieces. For the connection of PEEK capillaries. (MCS and sample degasser) Material: PEEK 2 6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 9 Accessories Order no. Description 6.2816.020 Syringe 10 mL with Luer connection For different usage in IC and VA Material: PP Length (mm): 102 Volume (mL): 10 1 6.2816.040 Purging needle With PTFE tubing and Luer connection. For syringes. For aspirating eluents. 1 6.2821.090 Aspiration filter Pore size 20 µm. Set of 5 pieces. For 6.1834.000 aspiration tubing and 6.1821.040 and 6.1821.050 filter tubes. Material: PE Outer diameter (mm): 9.5 Length (mm): 35.5 1 6.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.2 Optional accessories Qty. 1 Order no. Description 6.2837.000 CO2 adsorption cartridge Adsorption cartridge for purifying the air. 2 6.2837.010 H2O adsorption cartridge To CO2 Suppressor. Water adsorption cartridge for the aspirated air. 9.2 Optional accessories 2.881.0030 881 Compact IC pro – Anion – MCS Order no. Description 2.850.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Order no. Description 6.2617.040 Tool for piston seal Macro 9 Accessories For removing and assembling the piston seal for Macro pump heads 6.2741.040 PE/PTFE piston seal Macro For all Macro pump heads 6.2824.130 Macro pump head PEEK Macro pump head for intelligent IC instruments, flow range 0.1...20 mL/min, maximum pressure 12.5 MPa. Material: PEEK (metal-free) 6.6059.221 MagIC Net™ 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.2 Optional accessories Order no. Description uation and monitoring as well as report generation of ion chromatographic analyses.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Index Numbers/Symbols 6.2821.090 aspiration filter ...... 69 (6.2821.130) Filter .................... 80 A Accessories ............................. 112 Optional ........................... 122 Scope of delivery .............. 112 Adsorber cartridges Connection ........................ 56 Ambient conditions ................ 101 Aspiration filter 6.2821.090 ...... 69 Aspiration tubing for eluent ...... 25 B Baseline Conditioning ...................... 65 Unstable ..............
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Eluent degasser .................. 29 Guard column .................... 59 High pressure pump ........... 31 Injection valve ............ 39, 103 Leak sensor ........................ 20 MCS ................................... 54 MSM .................................. 51 Peristaltic pump .................. 47 Pulsation absorber .............. 36 Pump tubings ..................... 47 Sample degasser ................. 37 Separation column ............. 60 Suppressor ..............
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Eluent degasser ................ 102 High pressure pump ......... 102 Instrument ........................ 101 Interfaces ......................... 105 MCS ................................. 105 MSM ................................ 104 Peristaltic pump ................ 104 Sample degasser ............... 103 Temperature ........................... 101 Test Safety specification ........... 106 Transfer time ............................
