882 Compact IC plus 882 Compact IC plus – Cation Manual 8.882.
Metrohm AG CH-9101 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com 882 Compact IC plus 882 Compact IC plus – Cation 2.882.0010 Manual 8.882.8011EN 05.
Teachware Metrohm AG CH-9101 Herisau teachware@metrohm.com This documentation is protected by copyright. All rights reserved. Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above. Documentation in additional languages can be found on http://products.metrohm.com under Literature/Technical documentation.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents Table of contents 1 Introduction 1 1.1 Instrument description ......................................................... 1 1.2 Intended use ......................................................................... 2 1.3 About the documentation ................................................... 2 1.3.1 Symbols and conventions ........................................................ 2 1.4 Safety instructions .....................................................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 3.12 Injection valve ..................................................................... 31 3.12.1 Connecting the injection valve ............................................... 31 3.12.2 Mode of operation of the injection valve ............................... 33 3.12.3 Selecting the sample loop ...................................................... 34 3.13 Connecting the instrument ................................................ 34 3.13.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 6 Troubleshooting 6.1 Problems and their solutions ............................................. 62 7 Technical specifications Reference conditions .......................................................... 65 7.2 Instrument ........................................................................... 65 7.3 Leak sensor ......................................................................... 65 7.4 Ambient conditions ........................................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures Table of figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Figure 12 Figure 13 Figure 14 Figure 15 Figure 16 Figure 17 Figure 18 Figure 19 Figure 20 Figure 21 Figure 22 Figure 23 Figure 24 Figure 25 Figure 26 Figure 27 Figure 28 Figure 29 VI ￭￭￭￭￭￭￭￭ Front 882 Compact IC plus – Cation .................................................. 6 Rear 882 Compact IC plus – Cation ....................................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction 1 Introduction 1.1 Instrument description The instrument 882 Compact IC plus – Cation is one of the model versions of the 882 Compact IC plus line of instruments manufactured by the Metrohm Company. The 882 Compact IC plus line of instruments is distinguished by: ￭ ￭ ￭ ￭ ￭ ￭ the intelligence of its components, which are able to monitor and optimize all functions and to provide documentation according to FDA requirements. its compact style of construction.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.2 Intended use Pulsation absorber The pulsation absorber protects the separation column from damage caused by pressure fluctuations when switching the injection valve, and reduces interfering pulsations during highly sensitive measurements. Injection valve The injection valve connects the eluent and sample path through rapid and precise valve switchover. A precisely measured amount of sample solution is injected and rinsed with eluent onto the separation column.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction Warning This symbol draws attention to a possible hazard due to electrical current. Warning This symbol draws attention to a possible hazard due to heat or hot instrument parts. Warning This symbol draws attention to a possible biological hazard. Caution This symbol draws attention to a possible damage of instruments or instrument parts. Note This symbol marks additional information and tips. 1.4 Safety instructions 1.4.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.4 Safety instructions Warning Never open the housing of the instrument. The instrument could be damaged by this. There is also a risk of serious injury if live components are touched. There are no parts inside the housing which can be serviced or replaced by the user. Mains voltage Warning An incorrect mains voltage can damage the instrument. Only operate this instrument with a mains voltage specified for it (see rear panel of the instrument).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.4.4 1 Introduction Flammable solvents and chemicals Warning All relevant safety measures are to be observed when working with flammable solvents and chemicals. ￭ ￭ ￭ ￭ 1.4.5 Set up the instrument in a well-ventilated location (e.g. laboratory flue). Keep all sources of flame far from the workplace. Clean up spilled fluids and solids immediately. Follow the safety instructions of the chemical manufacturer.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.1 Front 2 Overview of the instrument 2.1 Front 8 7 6 1 5 2 Figure 1 3 4 Front 882 Compact IC plus – Cation 1 High pressure pump 2 Purge valve 3 Inline filter 4 Pulsation absorber 5 Injection valve 6 Column chamber 7 Column holder With column recognition. 8 Detector chamber Space for the detector.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.2 Rear 1 Knurled screws For fastening the removable rear panel. 2 Mains switch For switching the instrument on and off. I = On O = Off 3 Mains connection socket For connecting the mains cable. 4 PC connection socket For connecting the instrument to the computer with the USB cable (6.2151.020). 5 USB connectors 2 USB connectors labeled with USB 1 and USB 2. 6 MSB connectors 2 MSB connectors for connecting MSB devices. Labeled with MSB 1 and MSB 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Installation 3.1 About this chapter The Installation chapter contains: ￭ ￭ ￭ ￭ 3.2 this overview. a brief set of instructions for the initial installation of the 882 Compact IC plus – Cation. At each step you will find cross-references to more detailed installation instructions for individual components, should you require such aids. an installation diagram (see Figure 3, page 12), showing a completely installed 882 Compact IC plus – Cation.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.2 Initial installation ￭ ￭ Connect the leak sensor (see Chapter 3.6.2, page 16). Connect the drainage tubings (see Chapter 3.6.3, page 17). 3 Connecting the eluent path ￭ ￭ Lead the eluent aspiration tubing (6.1834.080) out of the instrument through a capillary feed-through and connect it with the eluent bottle (see Chapter 3.8, page 21). Connect the column inlet capillary (6.1831.150) and the detector inlet capillary with one another using a coupling (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation ￭ ￭ Connect the separation column (see Chapter 3.15, page 37) – Connect the inlet oft the separation column either with the end of the column input capillary using a short pressures screw (6.2744.070). OR Connect the inlet of the separation column with the guard column (if used) (see leaflets to the separation column and the guard column) – Fasten the detector inlet capillary to the output of the separation column using a short pressure screw (6.2744.070).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 1 Eluent aspiration tubing (6.1834.080) Connected to the high pressure pump. 2 Column input capillary (6.1831.150) Connected to the injection valve. 3 Detector inlet capillary 4 Detector outlet capillary 5 Sample aspirating capillary Connected to the injection valve. 6 Sample outlet capillary Connected to the injection valve. 7 PEEK pressure screws, short (6.2744.070) 3.4 Setting up the instrument 3.4.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.5 Capillary connections in the IC system Pressure screws 4 1 Figure 4 2 3 Connection of capillaries with pressure screws 1 PEEK pressure screw (6.2744.014) Use on the injection valve. 2 Connection capillary 3 PEEK pressure screw, short (6.2744.070) For use on the high pressure pump, the purge valve, the inline filter, the pulsation absorber, the guard column and the separation column. 4 PEEK pressure screw, long (6.2744.090) Use on special components.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation ￭ PEEK capillaries (6.1831.030) with an internal diameter of 0.75 mm for sample handling in the ultra trace range. Caution For the capillary connections between the injection valve and detector , PEEK capillaries with an internal diameter of 0.25 mm must be used. These are already connected to a newly delivered instrument. PTFE capillaries (polytetrafluoroethylene) PTFE capillaries are transparent and enable visual tracing of the liquids to be pumped.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.6 Installations on the rear of the instrument Colored sleeves for PEEK capillaries The enclosed set of varicolored sleeves for PEEK capillaries (6.2251.000) serves to easily differentiate the various flows of liquid in the system through color coding. Each capillary leading a given liquid (e. g. eluent) can be highlighted with sleeves of the same color.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Connecting the leak sensor Connect the leak sensor as follows: 1 Pull out the leak sensor connection cable (5-3) from the base tray. 2 Plug the leak sensor connector plug (5-2) into the leak sensor connection socket (5-1) on the rear of the instrument. 1 2 Figure 5 leak sensor – plugging in 1 Leak sensor connection socket Is labeled with Leak Sensor. 3 Leak sensor connection cable Extractable. Coiled up in the base tray. 3.6.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.6 Installations on the rear of the instrument 1 2 3 4 5 6 7 8 9 Figure 6 Drainage tubings 1 Drainage tubing connection For draining escaped liquid from the cover. 2 Drainage tubing Section of the 6.1816.020 silicon tubing. For draining escaped liquid from the cover. 3 Drainage tubing connection For draining escaped fluid from the detector chamber. 4 Drainage tubing Section of the 6.1816.020 silicon tubing. For draining escaped fluid from the detector chamber.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Installing drainage tubings Proceed as follows to install the drainage tubings: 1 Connect drainage tubing (6-2) to the drainage tubing connection (6-1) and shorten to the required length. 2 Connect drainage tubing (6-4) to the drainage tubing connection (6-3) and shorten to the required length. 3 Connect drainage tubing (6-2) and drainage tubing (6-4) to the Y connector (6-5).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.7 Capillary and cable feed-throughs 1 6 2 3 7 4 5 3 1 Figure 7 2 Capillary and cable feed-throughs 1 Capillary feed-through For feeding capillaries from the front to the rear of the instrument. 2 Capillary feed-through Fro feeding capillaries from the front to the right side of the instrument. 3 Capillary feed-through For feeding capillaries from the front to the left side of the instrument. 4 Luer connector For connecting a (6.2816.020) syringe.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3.8 Eluent 3.8.1 Connecting eluent bottle The eluent is aspirated out of the eluent bottle via the eluent aspiration tubing (8-1). The eluent aspiration tubing is connected to the high pressure pump (see Chapter 3.9, page 25) (see Chapter , page ). The tubing must be threaded through a suitable capillary feed-through (see Chapter 3.7, page 19) of the instrument before the other end can be equipped.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.8 Eluent 1 Eluent aspiration tubing (6.1834.080) 2 Tubing nipple From accessories set (6.1602.160). 3 O-ring From accessories set (6.1602.160). 4 Bottle attachment From accessories set (6.1602.160). 3 Mounting aspiration filter ￭ Insert filter holder (9-1) into the aspiration filter (9-2) and screw tight. 1 Figure 9 1 2 Mounting aspiration filter 2 Filter holder From accessories set (6.2744.210). Aspiration filter (6.2821.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation ￭ ￭ ￭ ￭ Figure 11 Slide the tubing weighting (10-3) onto the eluent aspiration tubing (10-1). Slide the clamping screw (10-4) onto the eluent aspiration tubing (10-1). Insert eluent aspiration tubing (10-1) into the aspiration filter (10-5). The end of the tubing should approximately reach to the center of the aspiration filter. Screw together clamping screw (10-4) and filter holder (9-1). Eluent aspiration tubing fully equipped.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.8 Eluent 1 14 2 13 3 12 11 4 10 5 1 9 Figure 12 8 7 6 Eluent bottle – connected 2 Eluent aspiration tubing (6.1834.080) For aspirating the eluent. Pre-installed. 2 Adsorber tube (6.1609.000) 3 Cotton 4 CO2 adsorber Adsorbs CO2 from the air (e.g. Merck soda lime with indicator, no. 6839.10). 5 Eluent 6 Aspiration filter (6.2821.090) 7 Filter holder From accessories set (6.2744.210). 8 Clamping screw From accessories set (6.2744.210).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.9 3 Installation High pressure pump The intelligent and low pulsation high pressure pump pumps the eluent through the system. It is equipped with a chip on which its technical specifications and "life history" (operating hours, service data, … ) are saved. The purge valve is used for deaerating (see Chapter 3.9.2, page 27) the high pressure pump. 3.9.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.9 High pressure pump 1 Connection capillary PEEK capillary, connects main piston and auxiliary piston. 2 PEEK pressure screw, short (6.2744.070) 3 Outlet valve holder 4 Pump head (6.2824.110) 5 Fastening screws For fastening the pump head. 6 Inlet valve holder 7 Pump head inlet capillary PEEK capillary at the input of the pump head. 8 Pressure screw For connecting a PEEK capillary to the coupling (13-9).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 3 Installation Pressure screw For connecting the coupling (14-2) to the pump head inlet capillary (13-7). 2 Coupling (6.2744.230) For connecting the eluent connection capillary (14-4) to the input of the high pressure pump. 4 Eluent aspiration tubing Eluent aspiration tubing (6.1834.080) or (6.1834.090). Can be ordered together with the coupling under the number (6.2744.230).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.9 High pressure pump 5 4 5 6 3 7 2 5 1 Figure 15 Deaerate the high pressure pump 1 Syringe 10 mL (6.2816.020) For aspirating the eluent. 2 Luer connector Part of the purging needle (6.2816.040) 3 Purging needle (6.2816.040) 4 Deaerating capillary 5 PEEK pressure screws, short (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 5 Aspirating eluent ￭ Aspirate with the syringe (15-1) until bubble-free eluent flows into the syringe. 6 Completing deaerating ￭ ￭ ￭ ￭ 3.10 Switch off high pressure pump. Close rotary knob (15-7). Remove syringe (15-1) from the Luer connector (15-2). Pull the purging needle (15-3) out of the deaerating capillary (15-4). Inline filter Between the purge valve and the pulsation absorber the inline filter (6.2821.120) is installed as protection against particles.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.11 Pulsation absorber 1 Figure 16 2 3 2 4 Connecting the inline filter 1 Connection capillary Connects the purge valve with the inline filter 2 PEEK pressure screws, short (6.2744.070) 3 Inline filter (6.2821.120) Protects against particles. 4 Connection capillary Connects the inline filter with the pulsation absorber. 1 Screw on the connection capillary running from the purge valve to the input side of the inline filter using a pressure screw (6.2744.070).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 6 2 5 1 3 4 Figure 17 Pulsation absorber – Connection 1 Connection capillary Connection to the inline filter. 2 Fastening screws 3 PEEK pressure screws, short (6.2744.070) 4 Holder for pulsation absorber 5 Pulsation absorber (6.2620.150) 6 Connection capillary Connection to the injection valve. 3.12 Injection valve The injection valve connects the eluent and sample path.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.12 Injection valve 6 5 7 7 2 1 4 5 3 2 6 7 1 7 4 3 Figure 18 Injection valve – connected 1 Injection valve 2 Sample loop Connected to connectors 3 and 6. 3 Connection capillary Connected to connector 4. Carries eluent to the injection valve. 4 Connection capillary (column inlet capillary) Connected to connector 5. Carries eluent to the separation column. 5 Connection capillary Connected to connector 1. Carries sample to the injection valve.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.12.2 3 Installation Mode of operation of the injection valve The injection valve (see Figure 19, page 33) can adopt two valve positions - FILL and INJECT. Switching back and forth between the two valve positions determines whether the sample path or the eluent path is guided through the sample loop. The following figure provides a schematic display of the flow paths of the two valve positions.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.13 Connecting the instrument 3.12.3 Selecting the sample loop The amount of sample solution injected depends on the volume of the sample loop. The choice is made on the basis of the application. The following sample loops are normally used: Cation determination 10 µL Anion determination with suppression 20 µL Anion determination without suppression 100 µL 3.13 Connecting the instrument 3.13.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 2 Switching on the instrument Switch on the instrument using the mains switch . After switching on, the LED on the front of the instrument flashes while a system test is carried out and the connection to the software is established. Once the system test is complete and the connection to the software has been established, the LED lights up continuously. 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 Guard column Note Metrohm recommends, always to work with guard columns. These protect the separation column and can be exchanged regularly. Connecting and rinsing the guard column 1 Connecting the guard column Caution When inserting the guard column, always ensure that it is inserted correctly corresponding to the flow direction (if indicated). ￭ ￭ ￭ Remove sealing caps and/or stoppers from the guard column.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.15 3 Installation Separation column The intelligent separation column (iColumn) is the heart of the ion chromatographic analysis. It separates the different components corresponding to their interactions with the column. Metrohm separation columns are equipped with a chip on which their technical specifications and their history (first use / setting up, operating hours, injections, …) are saved.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.15 Separation column Connecting and rinsing the separation column 1 Connect the separation column Caution When inserting the columns, always ensure that these are correctly inserted corresponding to the flow direction indicated. ￭ ￭ Remove stoppers from the separation column. Attach the guard column to the input of the separation column. OR Connect the input of the separation column to the outlet capillary of the guard column, using the PEEK pressure screw (6.2744.070) included.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Start-up 4 Start-up The chapter Start-up is divided into 2 sections: 4.1 Initial start-up The initial start-up is carried out during the initial installation. Conditioning Conditioning is carried out as a final installation step and each time after the system is started. Initial start-up The initial start-up is carried out during the initial installation. The entire system is rinsed before guard column and separation column are installed.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.2 Conditioning The instrument is now ready for the installation of the columns. 4.2 Conditioning After the installation and after switching on the instrument, the system must be conditioned with eluent until a stable baseline is reached. Note After a change of eluent (see Chapter 5.4.2.3, page 45), the conditioning time can lengthen considerably.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Start-up 4 Conditioning the system Rinse the system with eluent until the required stability of the baseline is attained (normally 30 minutes). The instrument is now ready for measuring samples.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1 General notes 5 Handling and maintenance 5.1 General notes 5.1.1 Care Warning The instrument housing must not be opened by untrained personnel. The instrument requires appropriate care. Excess contamination of the instrument may result in functional disruptions and a reduction in the service life of the sturdy mechanics and electronics.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1.3 5 Handling and maintenance Operation Caution In order to avoid disturbing temperature influences, the entire system including the eluent bottle must be protected against direct sunlight. 5.1.4 Shutting down If the instrument is not used for a longer period, the whole IC system (except the columns) must be rinsed salt free with methanol/ultrapure water (1:4), in order to prevent eluent salts from forming crystals which may cause subsequent damage.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.3 Door 5.3 Door Caution The door is made of PMMA (polymethylmetacrylate). It must never be cleaned with abrasive media or solvents. Caution Never use the door as a handle. 5.4 Eluent 5.4.1 Production The chemicals used for the production of eluents should have a degree of purity of at least "p.a.". Only ultrapure water (resistance > 18.2 MΩ*cm) may be used for dilution (this generally applies for reagents which are used in ion chromatography).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.4.2 5.4.2.1 5 Handling and maintenance Operation Supply bottle The supply bottle with the eluent must be connected as indicated in chapter 3.8.1, page 21. This is above all important for eluents with volatile solvents (e.g. acetone). Moreover, condensation must also be prevented in the eluent bottle. Drop formation can change the concentration ratio in the eluent.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump Caution In order to spare the pump seals, the pump should not be operated dry. Therefore ensure that the eluent supply is correctly connected and that there is enough eluent in the eluent bottle each time before switching on the pump. 5.5.2 Maintenance Caution Maintenance work on the high pressure pump may not be carried out unless the instrument is switched off.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Handling and maintenance Cleaning/replacing the zirconium oxide piston Clean one piston after the other as follows: 1 Removing the piston cartridge from the pump head Loosen the piston cartridge with a wrench and unscrew from the pump head by hand.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump ￭ Remove the backup ring from the pump head and lay to the other parts. 1 Figure 21 2 3 4 5 6 7 8 Components of the piston cartridge 1 Piston cartridge screw 2 Retaining washer 3 Zirconium oxide piston with piston shaft Order number: 6.2824.070 4 Spring retainer 5 Spring Order number: 6.2824.060 6 Inner plastic sleeve Protects from metallic abrasion.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Handling and maintenance for removing the old piston seal and a sleeve for inserting the new piston seal. 1 2 Figure 22 1 Tool for piston seal 2 Pin Tip for removing the old piston seal. Sleeve Sleeve for inserting the new piston seal. Caution Screwing the special tool for the piston seal (6.2617.010) into the piston seal destroys this completely! 1 Removing the piston seal Caution Avoid touching the sealing surface in the pump head (13-4) with the tool.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump 1 2 Figure 23 1 Removing the piston seal 2 Piston seal Tool for piston seal Pin of the tool. 2 Inserting the new piston seal into the tool Insert the new piston seal tightly by hand into the recess of the sleeve of the tool for the piston seal (22-2). The sealing springs must be visible from the outside. 1 2 Figure 24 1 Tool for piston seal (6.2617.010) Sleeve for inserting the new piston seal.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Handling and maintenance 1 2 Figure 25 Inserting the piston seal into the pump head 4 Replacing the piston cartridge Screw the assembled piston cartridge back into the pump head and tighten, first by hand, then additionally by approx. 15° with a wrench. Cleaning the inlet valve and outlet valve 1 Removing valves ￭ ￭ Unscrew the connection capillary for the auxiliary piston (13-1) from the outlet valve holder.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump 1 Outlet valve holder 2 Outlet valve Order number: 6.2824.160 3 Inlet valve Order number: 6.2824.170 4 Inlet valve holder 2 Cleaning undissected valve Clean contaminated or blocked valves initially without dismantling them completely. ￭ ￭ Rinse the valve in eluent flow and counterflow direction using a spray bottle filled with ultrapure water, RBS solution or acetone.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Handling and maintenance 1 2 3 4 Figure 27 Dismantling valve 1 Needle For pushing the valve components out of the valve housing. 2 valve 3 Recess For collecting the valve components. 4 Holder The components of the valve are collected in the recess of the holder. Note The components of the valve are very small. In order not to lose them, put the components into a dish.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 High pressure pump 1 Figure 28 2 3 4 5 5 6 6 7 9 8 8 9 7 10 10 Components of the inlet valve and outlet valve 1 Inlet valve (6.2824.170) 2 Outlet valve (6.2824.160) 3 Inlet valve housing 4 Outlet valve housing 5 Sealing ring (black) 6 Sleeve 7 Sapphire sleeve The shiny side must point to ruby ball. 8 Ruby ball 9 Ceramic holder for ruby ball 10 Seal The larger opening must point outwards.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Handling and maintenance ￭ ￭ ￭ Place over the valve housing and hold it tightly. By tilting the tool, the valve components slide into the valve housing. Press the seal by hand well on the valve housing. 6 Checking the flow direction Rinse the valve in the direction of the arrow on the valve housing and check wether liquid is escaping on the other end. If this is not the case, the valve has to be dismantled again and be reassembled correctly (see Figure 28, page 54).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.6 Inline filter Mounting the pump head Note To prevent the pump head from being positioned the wrong way, it is provided with different bore hole depths for the fastening bolts, i. e. a fastening bolt is longer than all others. The bore hole with the greatest depth must therefore be assigned to the longest bolt. If this is not the case, the pump will not function perfectly. 1 Mount the pump head on the pump again using the four fastening screws (13-5).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Handling and maintenance Changing the filter The flow must be stopped before changing the filter. 1 Removing the inline filter ￭ Unscrew the pressure screws (29-1) from the inline filter. 2 Unscrewing the filter screw ￭ Screw the filter screw (29-4) out of the filter housing (29-2) with the aid of two adjustable wrenches (6.2621.000). 3 Inserting the filter ￭ ￭ Remove the old filter (29-3) using tweezers.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.7 Injection valve 5.7 Injection valve 5.7.1 Protection To prevent contamination of the injection valve, a 6.2821.120 inline filter (see Chapter 3.10, page 29) should be mounted between the high pressure pump and the pulsation damper. 5.8 Inline sample preparation To protect the separation column (see Chapter 3.15, page 37) against foreign particles which can affect the separating efficiency, we recommend that all samples undergo a microfiltration (filter 0.45 µm).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Handling and maintenance 2 Aspirating the sample and measuring time Aspirate a sample typical for the later application and use a stop watch to measure the time required by the sample to travel from the sample vessel to the end of the sample loop. The time measured corresponds to the "transfer time". The rinsing time should be at least 3 times the transfer time.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.10 Separation column 5.10 Separation column 5.10.1 Separating efficiency Which analysis quality can be attained, depends to a great extent on the separating efficiency of the separation column used. The separating efficiency of the selected separation column must be sufficient for the analysis problems present. If difficulties occur, you should always first check the quality of the separation column by recording a standard chromatogram.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.10.4 5 Handling and maintenance Regeneration Note The regeneration is considered as the last measure, and not to be carried out regularly. If the separating properties of the column have deteriorated, the column can be regenerated according to the specifications of the column manufacturer. In the case of separation columns available from Metrohm, the specification for regeneration can be found on the leaflet provided along with each column. 5.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Problems and their solutions 6 Troubleshooting 6.1 Problems and their solutions Problem Cause Remedy Marked drop in pressure Leak in the system. Check all capillary connections and seal leaks, if necessary (see Chapter 3.5, page 13). Marked rise in pressure Inline filter (6.2821.120) blocked. Replace the filter (6.2821.130) (see Chapter 5.6, page 56). Guard column – blocked. Replace guard column (see Chapter 3.14, page 35). Separation column – blocked.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Problem 6 Troubleshooting Cause Remedy Eluent – Blockage in the eluent path. Check the eluent path. High pressure pump – defective piston seals. Replace (see Chapter 5.5.2, page 46) piston seals . Pulsation absorber not connected. Connect pulsation absorber (see Chapter 3.11, page 30). Pulsation absorber not connected. or defective. Connect pulsation absorber (see Chapter 3.11, page 30). or replace it. Background conductivity too high Incorrect eluent.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Problems and their solutions Problem Cause Remedy Separation column – dead volume at column head. ￭ ￭ Precision problems significant scattering of the measured values Unexpected change to the retention times in the chromatograms Injection valve – sample loop. Check installation of the sample loop (see Chapter 3.12.1, page 31). Sample – rinsing volume too low. Increase rinsing time (see Chapter 5.9, page 58). Injection valve – defective. Request Metrohm Service.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7 Technical specifications 7 Technical specifications 7.1 Reference conditions The technical data listed in this Chapter refers to the following reference conditions: Ambient temperature +25 °C (± 3 °C) Instrument status > 40 minutes in operation (equilibrated) 7.2 Instrument IC system ￭ ￭ Metal-free IC system Compact system with modular design Material Painted polyurethane hard foam without CFCs, fire class V0 Operating pressure range ￭ Intelligent components 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.5 Housing 7.5 Housing Dimensions Width 302 mm Height 562 mm Depth 368 mm Material of base tray, housing and covering plate Polyurethane hard foam (PUR) with flame retardation for fire class UL94V0, CFC-free, coated Operating elements Indicators LED for power display On/Off switch On the rear panel of the instrument 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Minimum pressure limit 7 Technical specifications ￭ The pump is automatically shut down at the first piston stroke above the maximum limit value ￭ Adjustable from 0…49 MPa (0…490 bar) The shutdown mechanism is inactive at 0 MPa The shutdown mechanism only becomes active 2 minutes after system start The pump is automatically shut down after 3 piston strokes below the minimum pressure limit ￭ ￭ ￭ 7.7 Injection valve Actuator time typ.100 ms Max.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.10 Safety specification Column recognition for an intelligent column Leak sensor 1 jack plug Further connections Auxiliary 1 DSUB 15-pin (female) Service 1 DSUB 15-pin (female) 7.10 Safety specification Design / Test ￭ ￭ ￭ ￭ 7.11 Emission Electromagnetic compatibility (EMC) ￭ ￭ ￭ ￭ ￭ Immunity ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ 68 ￭￭￭￭￭￭￭￭ EN/IEC 61010-1 UL 61010-1 CSA-C22.2 No.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.12 7 Technical specifications Weight Weight 882 Compact IC plus – Cation 17.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.1 Declaration of Conformity 8 Conformity and warranty 8.1 Declaration of Conformity This is to certify the conformity to the standard specifications for electrical appliances and accessories, as well as to the standard specifications for security and to system validation issued by the manufacturing company.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8 Conformity and warranty This instrument meets the requirements of the ETL Listed Mark for the North American market. It conforms to the electrical safety standards UL 61010-1 and CSA-C22.2 No. 61010-1. This product is listed in Intertek’s Directory of Listed Products. Manufacturer Metrohm Ltd., CH-9101 Herisau/Switzerland Metrohm Ltd.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8.3 Warranty (guarantee) Software development Software development occurs in terms of the software life cycle. Tests are performed to detect programming errors and to assess the program’s functionality in a laboratory environment. Components All components used in the Metrohm instruments have to satisfy the quality standards that are defined and implemented for our products. Suppliers of components are audited by Metrohm as the need arises.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8 Conformity and warranty Faults arising from circumstances that are not the responsibility of Metrohm, such as improper storage or improper use, etc. are expressly excluded from the warranty.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery 9 Accessories Note Subject to change without notice! 9.1 Scope of delivery 2.882.0010 882 Compact IC plus – Cation Qty. Order no. Description 1 1.882.0010 882 Compact IC plus – Cation 1 6.2122.0x0 Mains cable with C13 line socket IEC-60320-C13 Cable plug according to customer requirements. Switzerland: Type SEV 12 6.2122.020 Germany, …: Type CEE(7), VII 6.2122.040 USA, …: Type NEMA/ASA 6.2122.070 1 6.1602.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 9 Accessories Order no. Description 6.1609.000 Adsorber tube / large and bent For filling with adsorber material. Material: Height (mm): Inner diameter (mm): SGJ size: 1 6.1803.040 Glass 129 32 B-14/15 PTFE capillary 0.5 mm i.d. / 1 m Capillary for sample handling in IC. Material: PTFE Outer diameter (inches): 1/16 Inner diameter (mm): 0.5 Length (m): 1 1 6.1807.010 Y connector for tubing i.d.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery Qty. 2 Order no. Description 6.1816.020 Silicone tubing 6 mm i.d. / 1 m For drainage tubings Material: Outer diameter (mm): Inner diameter (mm): Length (m): 1 6.2023.020 Clip for SGJ 14/15 Clip for SGJ 14/15 Material: 1 6.2151.020 POM Cable USB A - USB B / 1.8 m USB connecting cable Length (m): 1 6.2251.000 Silicone rubber 9 6 1 1.8 Colored sleeves for capillaries Colored pieces of heat shrink tubing for capillary coding.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 9 Accessories Order no. Description 6.2617.010 Tool for piston seal For removing and assembling the piston seal for all standard pump heads 2 6.2621.000 Adjustable wrench Maximum opening: 20 mm. For IC instruments Length (mm): 150 1 6.2621.030 Length (mm): 1 6.2621.050 Hexagon key 4 mm 73 1/4 in. wrench For 1/4 in. screws.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery Qty. 1 Order no. Description 6.2621.080 Capillary cutter For plastic capillaries. For IC instruments Length (mm): 118 1 6.2621.100 Hexagon key 3 mm Hexagon key 3 mm. For IC Sample Processors Length (mm): 73 1 6.2626.000 Front drain nozzle Drain nozzle for Professional IC instruments to be mounted on the front of the instrument 2 6.2739.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 9 Accessories Order no. Description 6.2743.080 Stoppers for overflow, 5 pieces For Professional IC instruments 1 6.2744.014 Pressure screw 2x With UNF 10/32 connection. For the connection of PEEK capillaries Material: PEEK Length (mm): 26 1 6.2744.020 For IC instruments Material: Length (mm): 1 6.2744.040 Luer/UNF coupling PEEK 19 2 x UNF 10/32 coupling For connecting 1/16 in. capillaries.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9.1 Scope of delivery Qty. 1 Order no. Description 6.2744.070 Pressure screw short Short version. With UNF 10/32 connection. 5 pieces. For the connection of PEEK capillaries Material: PEEK Length (mm): 21 1 6.2744.210 Tubing adapter for aspiration filter For Professional IC instruments 1 6.2816.020 Syringe 10 mL with Luer connection For different usage in IC and VA Material: PP Length (mm): 102 Volume (mL): 10 1 6.2816.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 9 Accessories Order no. Description 6.2821.090 Aspiration filter Pore size 20 µm. Set of 5 pieces. For 6.1834.000 aspiration tubing and 6.1821.040 and 6.1821.050 filter tubes. Material: PE Outer diameter (mm): 9.5 Length (mm): 35.5 1 6.2821.130 Spare filter for inline filter Spare filter for inline filter. 9.2 Optional accessories 2.882.0010 882 Compact IC plus – Cation Order no. Description 2.850.
9.2 Optional accessories Order no. Description 6.2741.040 PE/PTFE piston seal Macro ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ For all Macro pump heads 6.2824.130 Macro pump head PEEK Macro pump head for intelligent IC instruments, flow range 0.1…20 mL/min, maximum pressure 12.5 MPa. Material: PEEK (metal-free) 6.5333.000 IQ/OQ Kit for IC The IQ/OQ Kit contains all parts and standard solutions required for IQ/OQ in ion chromatography. 6.6059.221 MagIC Net™ 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Order no. 9 Accessories Description all system components and the chromatography results. MagIC Net™ Professional complies fully with FDA Regulation 21 CFR Part 11 as well as GLP. Dialog languages: German, English, French, Spanish, Chinese, Korean, Japanese, et. al. 1 license. 6.6059.223 MagIC Net™ 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Index Numbers/Symbols (6.2821.130) Filter .................... 56 6.2821.090 aspiration filter ...... 45 A Accessories ............................... 74 Optional ............................. 81 Scope of delivery ................ 74 Ambient conditions .................. 65 Aspiration filter 6.2821.090 ...... 45 Aspiration tubing for eluent ...... 21 B Baseline Conditioning ...................... 41 Unstable ............................. 46 Blood ..............................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Leak-tightness .................... 39, 40 Loop see also "Sample loop" ....... 34 Pump head Maintenance ...................... 46 Purge valve ............................... 25 M Mains cable .............................. 34 Mains connection ............... 34, 67 Mains voltage ............................. 4 Maintenance High pressure pump ........... 45 Injection valve .................... 58 Pump head ......................... 46 Material ..................................
