940 Professional IC Vario 940 Professional IC Vario ONE/SeS/PP/HPG Manual 8.940.
Metrohm AG CH-9100 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com 940 Professional IC Vario 940 Professional IC Vario ONE/SeS/ PP/HPG 2.940.1540 Manual 8.940.8022EN 11.
Teachware Metrohm AG CH-9100 Herisau teachware@metrohm.com This documentation is protected by copyright. All rights reserved. Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above. Documentation in additional languages can be found on http://documents.metrohm.com.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents Table of contents 1 Introduction 1 1.1 Instrument description ......................................................... 1 1.2 Intended use ......................................................................... 3 1.3 Safety instructions ................................................................ 4 1.3.1 General notes on safety ........................................................... 4 1.3.2 Electrical safety .........................................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 3.13.2 Connecting the suppressor .................................................... 34 3.14 Peristaltic pump .................................................................. 38 3.14.1 Installing the peristaltic pump ................................................ 38 3.14.2 Mode of operation for the peristaltic pump ........................... 44 3.15 Metrohm CO2 Suppressor (MCS) ....................................... 45 3.15.1 General information on MCS ...
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 5.12.2 5.12.3 Taking care of the suppressor housing ................................... 88 Servicing the suppressor ........................................................ 88 5.13 Peristaltic pump .................................................................. 95 5.13.1 Notes on operating the peristaltic pump ................................ 95 5.13.2 Servicing the peristaltic pump ................................................ 95 5.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 8 Warranty (guarantee) 113 9 Accessories 115 Index VI ￭￭￭￭￭￭￭￭ 117 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures Table of figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Figure 12 Figure 13 Figure 14 Figure 15 Figure 16 Figure 17 Figure 18 Figure 19 Figure 20 Figure 21 Figure 22 Figure 23 Figure 24 Figure 25 Front ................................................................................................. 8 Rear ................................................................................................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction 1 Introduction 1.1 Instrument description The 940 Professional IC Vario is a professional ion chromatograph. It is distinguished by: ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ Its intelligence: All of the functions are monitored, optimized and documented in an FDA-compatible manner. Intelligent components, such as iColumns, save important data onto a chip. Its compact design: It has a small footprint. Its modularity: It provides flexibility for use in various applications.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.1 Instrument description Leak sensor The leak sensor detects leaking liquid that collects in the instrument's base tray. Liquid that leaks in the instrument is routed to the base tray using drainage tubing and detected there. Column thermostat The column thermostat regulates the temperature for the separation columns and the eluent, thereby providing stable measuring conditions. The interior of the column thermostat can be heated and cooled.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction Peristaltic pump The peristaltic pump is used for pumping sample and auxiliary solutions. It can rotate in both directions. Metrohm CO2 Suppressor (MCS) The Metrohm CO2 Suppressor (MCS) removes the CO2 from the eluent flow. This lowers the background conductivity, improves detection sensitivity and minimizes the injection peak and carbonate peak. Detector Metrohm offers a series of different detectors for various analysis tasks.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.3 Safety instructions The instrument can also be used as needed for the determination of cations or anions without chemical suppression. The present instrument is suitable for processing chemicals and flammable samples. Usage of the 940 Professional IC Vario therefore requires the user to have basic knowledge and experience in handling toxic and caustic substances.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction Mains voltage WARNING An incorrect mains voltage can damage the instrument. Only operate this instrument with a mains voltage specified for it (see rear panel of the instrument). Protection against electrostatic charges WARNING Electronic components are sensitive to electrostatic charges and can be destroyed by discharges.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.4 Symbols and conventions 1.3.5 Recycling and disposal This product is covered by European Directive 2002/96/EC, WEEE – Waste from Electrical and Electronic Equipment. The correct disposal of your old equipment will help to prevent negative effects on the environment and public health. More details about the disposal of your old equipment can be obtained from your local authorities, from waste disposal companies or from your local dealer. 1.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction NOTE This symbol highlights additional information and tips. 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.1 Front 2 Overview of the instrument 2.1 Front Figure 1 Front 1 Bottle holder Offers space for the eluent bottle and additional accessories. 2 Detector chamber Offers space for two embedded detectors and additional accessories. 3 Column holder For a third separation column outside the column thermostat. 4 Metrohm CO2 Suppressor (MCS) 8 ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument 5 Suppressor 6 Column thermostat With two column holders for two separation columns. 7 Inline filter 8 Sample degasser Removes air bubbles from the sample. 9 Injection valve 10 Pulsation absorber 11 Inline filter 12 Mixing valve 13 Pulsation absorber 14 Base tray With leak sensor. 15 Purge valve For deaerating the high-pressure pump. 16 High-pressure pump Pumps the eluent through the IC system.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.2 Rear 2.2 Rear Figure 2 Rear 1 Bottle holder Offers space for the eluent bottle and additional accessories. 2 Back panel Removable. Enables access to the detector chamber. 3 Drainage tubing connection For connecting the drainage tubing, which guides escaped liquids away from the detector chamber. 4 Transport locking screws For securing the vacuum pump(s) when transporting the instrument. Up to two vacuum pumps can be installed in an instrument.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument 5 Vacuum connection For connecting an Extension Module that has a degasser but not its own vacuum pump. This connection has to be firmly sealed with a stopper when not in use. 6 Exhaust opening Labeled Exhaust. For extracting the air from the vacuum chamber. 7 PC connection socket For connecting the instrument to the computer with the USB cable (6.2151.020). 8 USB connection sockets Labeled USB 1 and USB 2. For connecting USB devices.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.3 Feed-throughs for capillaries and cables Openings on the door Figure 3 1 Feed-throughs on the door Luer connector For connecting a capillary from inside and for inserting a syringe (6.2816.020) from outside. For manual sample injection. 2 Opening for capillaries For up to 3 capillaries. An opening for up to 3 capillaries is located on the door of the instrument.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument Openings on the back panel Figure 4 1 Openings for capillaries and cables Openings for capillaries 2 Openings for cables The removable back panel is outfitted with openings through which capillaries and cables can be lead out of the detector chamber. Ducts for capillaries There are ducts for capillaries between the instrument and base tray as well as between the instrument and the bottle holder.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.3 Feed-throughs for capillaries and cables Figure 5 14 ￭￭￭￭￭￭￭￭ Ducts for capillaries 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Installation 3.1 Setting up the instrument 3.1.1 Packaging The instrument is supplied in highly protective special packaging together with the separately packed accessories. Keep this packaging, as only this ensures safe transportation of the instrument. 3.1.2 Checks Immediately after receipt, check whether the shipment has arrived complete and without damage by comparing it with the delivery note. 3.1.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.2 Capillary connections in the IC system Also see: PEEK pressure screws video on the Internet http://ichelp.metrohm.com/maintenance.php?chapter=1_2. Connection capillaries PEEK capillaries and PTFE capillaries are used in the IC system. PEEK capillaries (polyetheretherketone) PEEK capillaries are temperature-resistant up to 100 °C, stable under pressure up to 400 bar (depending on the inner diameter), flexible, chemically inert and have an extremely smooth surface.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation ￭ ￭ The capillary ends do not have exactly flat edges. The two capillary ends do not completely meet. One prerequisite for dead-volume-free capillary connection is that both capillary ends are cut exactly flat. Therefore we recommend cutting PEEK capillaries only with a capillary cutter (6.2621.080). Also see: Cutting capillaries video on the Internet http://ichelp.metrohm.com/maintenance.php?chapter=1_1.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.3 Removing the handle 3.3 Removing the handle The instrument is equipped with a handle in order to make it easier to transport. The handle can be removed once the instrument is in place in the lab. Accessories You do not need any accessories for the following work steps. Removing the handle 1 Removing the handle ￭ ￭ 18 ￭￭￭￭￭￭￭￭ Unscrew the four knurled screws. Remove the handle. 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.4 3 Installation Removing transport locking screws To avoid damage to the drives for the high-pressure pump and the vacuum pump during transport, the pumps are secured with transport locking screws. These are located at the rear of the instrument and labeled with Transport security screws. Remove these transport locking screws before the initial start-up. Accessories For this step you need: ￭ Hex key 4 mm (6.2621.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.5 Connecting the drainage tubing and leak sensor Store the transport locking screws in a safe place. Reinsert the transport locking screws each time you transport the instrument a significant distance. CAUTION The pumps may be damaged if you transport the instrument without inserting the transport locking screws. 3.5 Connecting the drainage tubing and leak sensor The leak sensor detects leaking liquid that collects in the instrument's base tray.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Connecting the drainage tubing 1 Cut a piece of silicone tubing into three pieces using scissors: 2 × approx. 40 cm and 1 × 20 cm 2 Attach one end of the 40 cm long piece to the drainage tubing connection on the bottle holder. 3 Attach one end of the 20 cm long piece to the drainage tubing connection on the detector chamber. 4 Attach each of the loose ends of both pieces of silicone tubing to one end of the Y connector.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.6 Column thermostat Route the loose end into a waste container. 3.5.2 Connecting the leak sensor Plugging in the leak sensor connection cable The leak sensor connection cable is coiled up in the base tray. 1 Pull the leak sensor connection cable out of the base tray as far as needed. 2 Plug the plug for the leak sensor connection cable into the leak sensor connection socket (labeled Leak Sensor). 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.7 3 Installation Connecting the eluent bottle The eluent is aspirated out of the eluent bottle via the eluent aspiration tubing . The eluent aspiration tubing is installed on the input for the eluent degasser. The tubing has to be be fed out of the instrument through a suitable opening (see "Openings on the back panel", page 13) before the loose end can be connected to the eluent bottle.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.7 Connecting the eluent bottle 2 Mounting the tubing adapter and the aspiration filter Install the parts of the tubing adapter for aspiration filter (6.2744.210) accessory set: ￭ ￭ ￭ Start by pushing the tubing weighting onto the loose end of the eluent aspiration tubing. Then push the clamping screw onto the loose end of the eluent aspiration tubing. Lastly, push the filter holder onto the loose end of the eluent aspiration tubing and screw it onto the tubing nipple.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 4 Mounting the adsorber tube NOTE Depending on the eluent used, the adsorber tube (6.1609.000) must be filled differently: ￭ ￭ ￭ ￭ For alkaline eluents or eluents with a low buffer capacity: first a little cotton, then CO2 adsorber material. For all other eluents: only cotton. Remove the plastic cover from the large opening of the adsorber tube. Fill the adsorber tube and close it again using the plastic cover.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.8 Connecting the eluent degasser 3.8 Connecting the eluent degasser The high-pressure pump cannot generate uniform flow if the eluent contains small gas bubbles or dissolved gas. As a result, the baseline cannot be stabilized correctly. In order to achieve good measurement results, the eluent has to be degassed before it goes into the high-pressure pump. The eluent degasser is completely connected. No installation work is required. 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Figure 9 1 High-pressure pump with purge valve 2 Pump head Purge valve The high-pressure pump is completely connected. No installation work is required. 3.10 Installing an inline filter Inline filters reliably protect the separation column from potential contamination from the eluent. The small filter plates with 2 µm pore size can be replaced quickly and easily. They remove particles from the solutions, such as bacteria and algae. An inline filter (6.2821.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.11 Installing the pulsation absorber Figure 10 1 Inline filter 2 Input capillary Connected to the purge valve. Output capillary Connected to the pulsation absorber. The inline filter is completely connected. No installation work is required. 3.11 Installing the pulsation absorber The pulsation absorber is installed between the high-pressure pump and the injection valve.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.12 3 Installation Injection valve The injection valve connects the eluent path to the sample path. By a quick and precise switching of the valve a defined quantity of sample solution is injected and flushed to the separation column with the eluent. The quantity of injected sample solution is determined either by the volume of the sample loop or by an 800 Dosino, if Metrohm's intelligent partial loop injection techniques (MiPT) are being used.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.12 Injection valve Figure 12 1 Pressure screw Fastened to Port 6. 3 Pressure screw Fastened to Port 3. Exchanging the sample loop 2 Sample loop Exchanging the sample loop 1 Removing the existing sample loop ￭ ￭ Unscrew the pressure screws (6.2744.010) at Port 3 and Port 6. Remove the sample loop. 2 Installing a new sample loop ￭ ￭ 30 ￭￭￭￭￭￭￭￭ Fasten one end of the sample loop to Port 3 using a PEEK pressure screw (6.2744.010). Use the second PEEK pressure screw (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.13 3 Installation Suppressor The suppressor drive of the 940 Professional IC Vario can hold various rotors. The large rotors such as the SPM Rotor A (6.2835.000) and the MSM-HC Rotor A (6.2842.000) can be inserted directly. Small rotors like the MSM Rotor A (6.2832.000) and the MSM-LC Rotor A (6.2844.000) must first be fitted into the adapter (6.2842.020) which can then be inserted into the suppressor housing. A connecting piece (6.2835.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.13 Suppressor CAUTION The rotor may be destroyed during start-up if not inserted correctly. Therefore, follow the following instructions exactly. Inserting large rotors 1 Removing the union nut Loosen the union nut and remove it. 2 Inserting the rotor ￭ ￭ 32 ￭￭￭￭￭￭￭￭ Clean the sealing surface of the rotor with ethanol using a lintfree cloth.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation NOTE The rotor's sealing surface is approximately 4 mm inside the suppressor drive if the rotor is inserted correctly. If this is not the case, then the rotor has to be moved into the correct position from below using a sharp object (e.g. a screwdriver). 3 Inserting the connecting piece ￭ ￭ Clean the sealing surface of the connecting piece with ethanol using a lint-free cloth.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.13 Suppressor ￭ Insert the rotor into the adapter so that the tubing connections on the rear of the rotor fit into the corresponding recesses inside the adapter and one of the three holes of the rotor is visible in the slot of the adapter. 2 Inserting the adapter Insert the adapter into the suppressor drive just like a large rotor (see "Inserting large rotors", page 32). 3.13.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Recommended installation Alternative installation Installing bottles with auxiliary solutions Accessories To connect the bottles of the auxiliary solutions, you will need the following accessories: ￭ Accessories from the accessory kit: IC Vario/Flex SeS (6.5000.020) 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.13 Suppressor 3.13.2.1 Installing the eluent path The eluent path is connected with the capillaries in and out. 1 Connect the capillary labeled with in with the output of the separation column using a short pressure screw (6.2744.070). 2 Use a long pressure screw (6.2744.090) to connect the capillary labeled with out to the input of the MCS (see Chapter 3.15.2, page 46). 3.13.2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 1 Prepare a tubing cartridge of the peristaltic pump for the regeneration solution (see Chapter 3.14.1, page 38). 2 Fasten the capillary labeled regenerant to the outlet of the pump tubing using a pressure screw (6.2744.070). 3 Fasten the PTFE capillary from the bottle with the regeneration solution to the inlet of the pump tubing. 3.13.2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 Peristaltic pump Connecting the rinsing solution inlet to the peristaltic pump For this step you need the following accessories: ￭ ￭ ￭ ￭ ￭ Accessory kit: Flex/Vario: ChS (6.5000.030) Pump tubing (6.1826.420) Tubing olive with filter and lock (6.2744.180) Tubing olive (6.2744.034) Tubing cartridge of the peristaltic pump 1 Prepare a tubing cartridge of the peristaltic pump for the regeneration solution (see Chapter 3.14.1, page 38).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Order number Name Material Inner diameter Use 6.1826.330 Pump tubing LFL (orange/ white), 3 stoppers PVC (Tygon®) 0.64 mm No special applications. 6.1826.340 Pump tubing LFL (black/ black), 3 stoppers PVC (Tygon®) 0.76 mm For sample solution in inline dialysis. 6.1826.360 Pump tubing LFL (white/ white), 3 stoppers PVC (Tygon®) 1.02 mm For sample transfer. 6.1826.380 Pump tubing LFL (gray/ gray), 3 stoppers PVC (Tygon®) 1.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 Peristaltic pump Pump tubing Adapter 6.1826.390 (yellow/yellow) 6.1826.420 (orange/yellow) 6.1826.020 (blue/blue) 40 ￭￭￭￭￭￭￭￭ 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Installing the pump tubing For this step you need the following accessories: ￭ ￭ ￭ ￭ ￭ Tubing cartridge (6.2755.000) Pump tubing (6.1826.XXX) Coupling Olive/UNF 10/32 (6.2744.034) Pump tubing connection with safety device and filter (6.2744.180): Includes a locknut, 3 adapters and a tubing olive with filter holder. 2 × pressure screw, short (6.2744.070) 1 Connecting the pump tubing ￭ Attach the Coupling Olive/UNF 10/32 (6.2744.034) to the pump tubing inlet.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 Peristaltic pump ￭ Install the pump tubing connection with safety device and filter (6.2744.180) at the pump tubing outlet: – Push the locknut onto the pump tubing. – Push the appropriate adapter onto the pump tubing. – Place the tubing olive with the filter holder into the pump tubing so that the pump tubing is firmly in place; push the end of the pump tubing over at least the second notch of the olive. – Tighten it using the union nut.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Input Tighten the aspiration tubing (6.1803.020) to the tubing olive (6.2744.034) using a pressure screw (6.2744.070). Output Tighten the regenerant suppressor capillary to the pump tubing connection with safety device (6.2744.180) using a pressure screw (6.2744.070). Next step ￭ Connect the loose end of the aspiration capillary to the bottle with the regeneration solution.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.14 Peristaltic pump Setting the contact pressure correctly 1 ￭ ￭ ￭ ￭ Fully loosen the contact pressure lever , i.e. press it all the way down. Activate the peristaltic pump drive in the software. Raise the contact pressure lever one step at a time until liquid flows. When liquid starts flowing, raise the contact pressure lever by an additional two ratchet increments. The contact pressure is now set optimally. 3.14.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Figure 14 Peristaltic pump 1 Contact pressure lever 2 Tubing cartridges (6.2755.000) 3 Cartridge holder 4 Snap-action lever 5 Rollers 6 Roller hub 7 Mounting bolt 3.15 Metrohm CO2 Suppressor (MCS) 3.15.1 General information on MCS NOTE The Metrohm CO2 Suppressor (MCS) is used only in conjunction with conductivity detection with chemical suppression. The Metrohm CO2 Suppressor (MCS) removes the CO2 from the eluent stream.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.15 Metrohm CO2 Suppressor (MCS) ymer membrane. At the same time, the vacuum pump generates a vacuum and draws in the air from the outside. The pressure and concentration difference this creates in the degassing cell relative to inside the capillary causes the CO2 to be diffused out of the eluent stream. The ambient air is drawn in by a CO2 adsorption cartridge that filters out the CO2. 3.15.2 Connecting the MCS The MCS is connected between the suppressor and the detector.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Connection to the detector Connect the detector input capillary with a long pressure screw (6.2744.090) to the MCS output (labeled Out). CAUTION If the MCS is not used, the input and output must be sealed with the threaded stoppers (6.2744.220). 3.15.3 Installing adsorption cartridges The suctioned air has to have the lowest CO2 content possible in order for the CO2 to be removed from the eluent as efficiently as possible.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.15 Metrohm CO2 Suppressor (MCS) 1 Preparing the adsorption cartridge holder ￭ Push the 4 clips into the slots on the adsorption cartridge holder. 2 Connecting adsorption cartridges ￭ ￭ ￭ ￭ Attach the PVC tubing to the tip of the H2O adsorption cartridge. Insert the adapter into the other end of the PVC tubing. Insert the adapter into the star-shaped cap of the CO2 adsorption cartridge.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.16 3 Installation Installing the detector The 940 Professional IC Vario provides enough space for two detectors and additional accessories in the detector chamber. The detectors are available as accessories and are supplied with separate manuals. Placing the detector in the instrument Follow the instructions in the chapter Inserting the detector in the manual for the detector.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.17 Connecting the sample degasser (optional) Connecting the sample degasser CAUTION If the sample degasser is not used, the input and output must be sealed with threaded stoppers (6.2744.220). 1 Removing the threaded stoppers Remove and keep the threaded stoppers (6.2744.220) from the input and output of the sample degasser. 2 Connecting the output capillary ￭ ￭ Push a long pressure screw over the loose end of the capillary connected to port 1 of the injection valve.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.18 3 Installation Installing the high-pressure gradient module The outputs of the two high-pressure pumps are connected to the injection valve's eluent input using the mixing coil for high-pressure gradients (6.2758.000). Connect the two pieces of eluent aspiration tubing to an eluent bottle (see Chapter 3.7, page 23). 3.19 Connecting the instrument to a computer NOTE The instrument must be switched off when being connected to a computer.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.20 Connecting the instrument to the power supply Connecting the USB cable 1 Insert the USB cable into the computer connection socket on the rear of the instrument labeled PC. 2 Insert the other end into a USB port on the computer. 3.20 Connecting the instrument to the power supply WARNING The power supply unit must not get wet. Protect it from liquids. The power supply cable is three-core and provided with a plug with grounding.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Connecting the power supply cable 1 Inserting the power supply cable ￭ ￭ Insert the power supply cable into the instrument's power socket. Connect the power supply cable to the power supply. 2 Switching on the instrument Switch on the instrument using the power switch. After being switched on, the LED on the front of the instrument flashes. The instrument conducts a system test and establishes a connection to the software.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.21 Initial start-up Rinsing the IC system CAUTION The guard column and separation column must not be installed for the initial start-up. Make sure that a coupling (6.2744.040) is being used instead of the columns. 1 Preparing the software ￭ ￭ ￭ Start the MagIC Net computer program. Open the Equilibration tab in MagIC Net: Workplace ▶ Run ▶ Equilibration. Select (or create) a suitable method. Also see: MagIC Net tutorial and online help.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 4 Deaerating the high-pressure pump ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ Push the end of the purging needle (6.2816.040) over the end of the deaerating capillary on the purge valve. Insert the syringe (6.2816.020) in the Luer connector of the purging needle. Open the purge valve using the rotary knob (approx. ½ turn). Switch on the high-pressure pump in MagIC Net. Use the syringe to aspirate eluent until there are no more air bubbles in the aspirated eluent.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.22 Connecting and rinsing the guard column 3.22 Connecting and rinsing the guard column Guard columns protect the separation column and substantially increase its service life. Guard columns available from Metrohm are either actual guard columns or what are known as guard column cartridges that can be used together with a cartridge holder. The process for installing a guard column cartridge in the associated holder is described in the leaflet for the guard column.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ ￭ 3 Installation Guard column (suitable for the separation column) Connecting the guard column 1 Removing the coupling Remove the coupling installed between the column input capillary and the column output capillary for the initial start-up. 2 Preparing the guard column ￭ Remove the stopper and the sealing cap from the guard column.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.23 Connecting the separation column ￭ ￭ ￭ 3.23 Start manual control in MagIC Net and select the high-pressure pump: Manual ▶ Manual control ▶ Pump – Flow: in accordance with column leaflet – On Rinse the guard column with eluent for approx. 5 minutes. Use manual control in MagIC Net to stop the high-pressure pump again: Off. Connecting the separation column The intelligent separation column (iColumn) is the heart of the ion chromatographic analysis.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation NOTE The separation column may not be connected until after the instrument has already been put into operation once (see Chapter 3.21, page 53). The guard column and separation column have to be replaced by a coupling (6.2744.040) until then. 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.23 Connecting the separation column Connecting the separation column 1 Removing the stoppers CAUTION When inserting the column, ensure that it is inserted correctly based on the marked flow direction. ￭ Remove the stoppers from the separation column. 2 Installing the input of the separation column There are 3 possibilities: ￭ ￭ ￭ Attaching the bottom end of the separation column directly to the guard column.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 5 Installing the output of the separation column ￭ Fasten the column output capillary to the upper end of the separation column using a PEEK pressure screw (6.2744.070). 6 Inserting the separation column ￭ Insert the separation column with chip into the column holder until you hear it snap in place. The separation column is now detected by MagIC Net. 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.24 Conditioning ￭ Ensure that the eluent aspiration tubing is immersed in the eluent and there is enough eluent in the eluent bottle. 3 Starting equilibration ￭ ￭ Start the equilibration in MagIC Net: Workplace ▶ Run ▶ Equilibration ▶ Start HW. Visually inspect whether all capillaries and their connections from the high-pressure pump to the detector are leak-tight.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Operation 4 Operation The 940 Professional IC Vario – ONE/SeS/PP/HPG is operated solely using the MagIC Net software. You can find information on operating the software in the tutorial for MagIC Net or in the online help. 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1 IC system 5 Operation and maintenance 5.1 IC system 5.1.1 Operation CAUTION In order to avoid disruptive temperature influences, the entire system including the eluent bottle must be protected from direct sunlight. 5.1.2 Care The instrument requires appropriate care. Excess contamination of the instrument may result in functional disruptions and a reduction in the lifetime of the sturdy mechanical and electronic components.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1.4 5 Operation and maintenance Shutting down and starting back up If the instrument is not used for a long period, the whole IC system (without separation column) must be rinsed to be free of salts using methanol/ ultrapure water (1:4). This prevents eluent salts from crystallizing and then causing damage. Rinsing the IC system to be free of salts 1 In the software, stop the hardware and wait until the pressure in the high-pressure pump has dissipated.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.2 Capillary connections 5.2 Capillary connections All connections between injection valve, separation column and detector must be as short as possible, have a low dead volume and be completely leak-tight. The PEEK capillary downstream of the detector must be free of blockages. Only use PEEK capillaries with an inner diameter of 0.25 mm in the highpressure section between the high-pressure pump and the detector. 5.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 1 3 1 2 2 1 1 1 Figure 16 Column thermostat 1 Recesses For feeding capillaries into and out of the instrument. 3 Column holder With column recognition. For fastening the column. 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.1540) 2 Preheating grooves For regulating the temperature of the eluent.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 Handling the eluent Replacing the preheating capillaries In order to make threading the column input capillary into the column thermostat's preheating grooves easier, we recommend loosening the screws for the holder plate a little, but not removing them all the way. 1 Guide the column input capillary into the column thermostat via a suitable recess (16-1). 2 Push the column input capillary into the outermost of the two preheating grooves (16-2) from the bottom.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance The composition of the eluent plays a critical role in chromatographic analysis: 5.5.2 Concentration An increase in the concentration generally leads to shorter retention times and faster separation, but also to a higher background conductivity signal. pH pH changes lead to shifts in dissociation equilibria and thus to changes in retention times.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.7 Notes on operating the high-pressure pump Connecting the connecting tubing 1 ￭ ￭ 5.7 Insert the clamping screws on the connector and screw them in by hand. Tighten the clamping screws with the wrench. Notes on operating the high-pressure pump CAUTION The pump head is filled ex works with methanol/ultrapure water. Ensure that the eluent used is miscible with this solvent.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 5 Operation and maintenance Servicing the high-pressure pump Figure 17 High-pressure pump – parts 1 Pressure screw, short (6.2744.070) Fastened to the outlet valve holder. 2 Outlet valve holder 3 Fastening screw 4 Pump head 5 Inlet valve holder 6 Pressure screw, short (6.2744.070) Fastened to the inlet valve holder. 7 Eluent aspiration tubing connector Consists of a coupling with a pressure screw. 8 Pressure screw, short (6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump CAUTION Maintenance work on the high-pressure pump may not be carried out unless the instrument is switched off. Recommended procedure We recommend the following for the maintenance of the pump head: 1. 2. 3. 4. Service the inlet valve and the outlet valve. Remove the pump head. Service both pistons. Reinstall the pump head. You can find brief video sequences on the following maintenance steps on the Internet at http://www.metrohm.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Cleaning the outlet valve 1 Removing the outlet valve ￭ ￭ ￭ Unscrew the connection capillary to the auxiliary piston from the outlet valve holder (17-2) (1). Start by loosening the outlet valve holder with the adjustable wrench and then unscrew it by hand (2) and remove it. Remove the outlet valve from the outlet valve holder (3).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump NOTE The maximum treatment time in an ultrasonic bath is 20 s. Ultrasonic baths that last longer than this can damage the ruby ball inside the valve. The outlet valve has to be replaced if it remains clogged after the ultrasonic bath. 3 Reinserting the outlet valve into the pump head ￭ ￭ ￭ Insert the outlet valve into the outlet valve holder (the seal must be visible) (1).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Spare parts 5 Operation and maintenance If the inlet valve cannot be cleaned, you will need a new inlet valve (6.2824.170) for this step. Cleaning the inlet valve 1 Removing the inlet valve ￭ ￭ Start by loosening the inlet valve holder with the adjustable wrench (1) and then unscrew it by hand (2) and remove it. Remove the inlet valve from the inlet valve holder (3).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump NOTE The maximum treatment time in an ultrasonic bath is 20 s. Ultrasonic baths that last longer than this can damage the ruby ball inside the valve. The inlet valve has to be replaced if it remains clogged after the ultrasonic bath. 3 Reinserting the inlet valve into the pump head ￭ ￭ Insert the inlet valve into the inlet valve holder (the seal must be visible) (1).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Has the pressure dissipated? Is the instrument switched off? ￭ ￭ 1 Release the coupling from the pressure screw and seal it with a stopper. 2 Unscrew and remove the pressure screw on the pump head's output (17-8). 3 Unscrew and remove the four fastening screws (17-3) using the hex key. 4 Remove the pump head (17-4). Replacing the piston seal Carry out the following work on both pistons in turn. Servicing a piston consists of the following tasks: 1. 2.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump Figure 18 High-pressure pump – cross-section 1 Outlet valve holder 2 Outlet valve (6.2824.160) 3 Backup ring 4 Piston seal (6.2741.020) 5 Pump head 6 Inlet valve (6.2824.170) 7 Inlet valve holder 8 Zirconium piston (6.2824.070) 9 Piston cartridge Accessories For this step you need the following accessories: You can find these parts in the accessory kit: Vario/Flex Basic (6.5000.000). ￭ ￭ 78 ￭￭￭￭￭￭￭￭ Adjustable wrench (6.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 1 2 Figure 19 1 Tool for piston seal (6.2617.010) 2 Tip Spare part Sleeve For this step you need a new piston seal (6.2741.020). Replacing the piston seal Figure 20 1 Removing the piston cartridge from the pump head 2 Piston cartridge Backup ring 1 Removing the piston cartridge Loosen the piston cartridge (18-9) using the adjustable wrench and then unscrew it from the pump head by hand. Place it off to the side.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump 3 Removing the old piston seal CAUTION Screwing the (6.2617.010) special tool for the piston seal into the piston seal destroys this completely! CAUTION Avoid touching the sealing surface in the pump head with the tool! Only screw the tip (19-1) of the tool for piston seal far enough into the piston seal that the seal can be pulled out.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Optional: Cleaning/replacing the zirconium oxide piston If the baseline still remains unstable after replacing the valves and piston seal, the zirconium oxide piston in the high-pressure pump may be dirty or scratched. In that case, the zirconium oxide piston must have to be cleaned or replaced.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.8 Servicing the high-pressure pump Figure 22 Components of the piston cartridge 1 Piston cartridge screw 2 Retaining washer 3 Zirconium oxide piston (6.2824.070) 4 Spring retainer 5 Spring (6.2824.060) 6 Inner plastic sleeve Protects from metallic abrasion.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance You can find these parts in the accessory kit: Vario/Flex Basic (6.5000.000). 4 mm hex key (6.2621.030) ￭ Mounting the pump head NOTE To prevent the pump head from being mounted the wrong way, it has fastening bolts with different bore hole depths, i.e. one fastening bolt is longer than all others. The bore hole with the greatest depth must therefore be assigned to the longest bolt. 1 ￭ ￭ Push the pump head onto the four fastening bolts (1).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.9 Servicing the inline filter Connecting the input for the high-pressure pump 1 ￭ ￭ 5.9 Remove the stopper from the coupling. Tighten the coupling to the pressure screw located on the pump head input capillary (1). Reconnect the pump head output capillary to the pump head output (2). Servicing the inline filter NOTE You can find a video sequence for this task in the Multimedia Guide IC Maintenance or on the Internet at http://www.metrohm.com/com/ Support/Video/ICGeneral.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Removing the filter Figure 23 Inline filter – removing the filter 1 Filter housing Inline filter housing. Part of the accessories (6.2821.120). 3 Filter (6.2821.130) Packaging contains 10 pieces. 2 Filter screw Screw for the inline filter. Part of the accessories (6.2821.120). 1 Shutting off the flow Switch off the high-pressure pump in the software. 2 Removing the inline filter Unscrew the both pressure screws from the inline filter.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.9 Servicing the inline filter Inserting a new filter 1 Inserting a new filter ￭ Place the new filter flat in the filter housing (23-1) using tweezers. 2 Mounting the filter screw ￭ Screw the filter screw (23-2) back into the filter housing (23-1) and tighten by hand. Then use two adjustable wrenches (6.2621.000) to tighten it slightly. 3 Remounting the inline filter ￭ Screw pressure screws back onto the inline filter.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.10 5 Operation and maintenance Servicing the pulsation absorber CAUTION The pulsation absorber is maintenance-free and must not be opened. 5.11 Injection valve Maintenance on the injection valve is best performed by specialist personnel from Metrohm during annual service. 5.12 Suppressor 5.12.1 Notes for operating the suppressor To protect the suppressor against foreign particles or bacterial growth, a pump tubing connection with 6.2744.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12 Suppressor CAUTION The suppressor has to be regenerated (see Chapter 5.12.3.2, page 89), cleaned (see Chapter 5.12.3.3, page 90) or replaced (see Chapter 5.12.3.4, page 92) if the capacity of the suppressor is reduced or if the backpressure is high. 5.12.2 Taking care of the suppressor housing CAUTION The transparent suppressor housing can become fogged. The suppressor housing is made of PMMA (poly(methyl methacrylate)).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12.3.2 5 Operation and maintenance Regenerating the suppression rotor If the suppressor units are loaded with certain heavy metals (such as iron) or organic contamination for long periods, then the standard regeneration solution may no longer be able to completely remove them. This constantly reduces the capacity of the suppressor units, which results in reduced phosphate sensitivity in mild cases and a large increase in the baseline in severe cases.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12 Suppressor ￭ ￭ In the software, use the Step command to switch to the next suppressor unit and regenerate it as described above. As soon as all three suppressor units have been regenerated, disconnect the capillary labeled regenerant from the coupling. 3 Rinsing the suppressor After regeneration, each of the three suppressor units must be rinsed with degassed ultrapure water for about 15 minutes.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 2 Dismantling the suppressor ￭ ￭ ￭ Unscrew union nut (24-1) from the housing (24-4). Pull the connecting piece (24-2) out of the housing together with the the rotor (24-3). If rotor A gets stuck in the housing, you can knock it loose as follows: Put a pointed object into the slot in the housing and use it to knock rotor A loose. Detach the connecting piece from the rotor.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12 Suppressor ￭ ￭ Insert the rotor (24-3) into the housing (24-4) in such a way that the tubing connections on the rear of rotor fit into the corresponding recesses inside the housing and one of the three holes of the rotor is visible from below in the slot of the housing (24-5). The rotor's sealing surface is approximately 4 mm inside the housing if the rotor is inserted correctly.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Replacing parts of the suppressor 1 Disconnecting the suppressor from the IC system ￭ ￭ Switch off the instrument. Disconnect all capillaries of the suppressor from the IC system. 2 Dismantling the suppressor ￭ ￭ ￭ Unscrew union nut (24-1) from the housing (24-4). Pull the connecting piece (24-2) out of the housing together with the the rotor (24-3).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.12 Suppressor ￭ The rotor's sealing surface is approximately 4 mm inside the housing if the rotor is inserted correctly. If this is not the case, the rotor must be moved into the right position from below using a sharp object. 5 Cleaning the new connecting piece ￭ Clean the surface of the new connecting piece (24-2) with ethanol using a lint-free cloth.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 5.13 Peristaltic pump 5.13.1 Notes on operating the peristaltic pump The flow rate of the peristaltic pump depends on the drive speed (set using the software), the contact pressure and, above all, the inner diameter of the pump tubing. Depending on the application, different pump tubing is used. Select pump tubing that best matches your application (see Table 2, page 38).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.13 Peristaltic pump 5.13.2.2 Replacing the filter The filters inserted into the pump tubing connection with safety device and filter (6.2744.180) have to be replaced regularly. Maintenance interval We recommend replacing the filters (6.2821.130) (25-2) every 3 months. The filters have to be replaced more frequently if the backpressure is higher. Accessories For this step you need the following accessories: ￭ ￭ ￭ 1 filter from the spare filter set (6.2821.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance 5.14 Servicing the Metrohm CO2 Suppressor (MCS) 5.14.1 Replacing the CO2 adsorption cartridge Maintenance interval The CO2 adsorption cartridge (6.2837.000) loses capacity over time. In addition, it can become slightly clogged due to too much liquid. It has to be replaced regularly about every 6 months. Capacity loss The adsorption capacity of the CO2 adsorption cartridge is limited and decreases over time.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.15 Servicing the detector 2 Regenerating the adsorber material Allow the depleted adsorber material to dry overnight in an oven at 140 °C. The dried adsorber material once again has the original color and can be reused. Two H2O adsorption cartridges are provided so that the H2O adsorption cartridge can continue to work without interruption. 5.15 Servicing the detector Follow the maintenance instructions in the the detector's manual. 5.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance The time upon stopping the watch corresponds to the "transfer time". If the sample is injected automatically, the rinsing time has to be at least three times the transfer time. Checking the rinsing time You can determine if the applied rinsing time is sufficient by measuring the sample carry-over directly. Proceed as follows to do this: 1 Preparing two samples ￭ ￭ Sample A: A typical sample for the application. Sample B: Ultrapure water.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.17 Separation column representative) or on the Internet at http://www.metrohm.com in the Ion Chromatography product area. You can find information on special IC applications in the corresponding "Application Bulletins" or "Application Notes", which are available on the Internet at http:// www.metrohm.com in the Applications area or free of charge upon request from your responsible Metrohm representative. 5.17.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.18 5 Operation and maintenance Quality Management and qualification with Metrohm Quality management Metrohm offers you comprehensive support in implementing quality management measures for instruments and software. Further information on this can be found in the brochure "Metrohm Quality Management" available from your local Metrohm representative. Qualification Please contact your local Metrohm representative for support in qualification of instruments and software.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 6 Troubleshooting Problem Cause Remedy Marked drop in pressure. Leak in the system. Check all capillary connections and seal leaks, if necessary (see Chapter 3.2, page 15). The baseline has a large amount of noise. The eluent is not sufficiently degassed. Ensure that the connectors for the eluent degasser are firmly connected (see Chapter 5.6, page 69). The eluent path has a leak. Check the eluent path and fix the leak. High-pressure pump – Contaminated pump valves.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Problem 6 Troubleshooting Cause Remedy Note: 6.2821.180 pump tubing connection with filter must be used. Guard column – Blocked. Replace the guard column (see Chapter 3.22, page 56). Separation column – Blocked. ￭ ￭ Regenerate the separation column (see Chapter 5.17.4, page 100). Replace the separation column (see "Connecting the separation column", page 60). Note: Samples should always be microfiltered . The retention times in the chromatogram have changed unexpectedly.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Problem Cause Remedy Peristaltic pump – Pump tubing defective. Replace the pump tubing . Column chip contaminated. Clean the contact surfaces of the column chip with alcohol. Column chip is defective. 1. Save column configuration in MagIC Net™. 2. Notify Metrohm Service. Individual peaks are greater than expected. Sample – Sample carryover from the previous measurement. Check the rinsing time (see "Checking the rinsing time", page 99).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6 Troubleshooting Problem Cause Remedy ￭ Deaerate the high-pressure pump (see Chapter 3.21, page 53). Baseline increasing strongly. Suppressor – Reduced capacity. Regenerate the suppressor (see Chapter 5.12.3.2, page 89). SPM – Reduced capacity. Regenerate the SPM (see Chapter 5.12.3.2, page 89). Separation column – Diminished separating efficiency. ￭ Chromatograms have poor resolution Extreme spread of the peaks in the chromatogram.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.1 Reference conditions 7 Technical specifications 7.1 Reference conditions The technical specifications listed in this chapter refer to the following reference conditions: Ambient temperature +25 °C (± 3 °C) Instrument status Operating > 40 minutes (in equilibrium) 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.4 7 Technical specifications Housing Dimensions Width 365 mm Height 642 mm Depth 380 mm Operating elements Indicators LED standby indicator On/off switch On the rear of the instrument 7.5 Leak sensor Electronic, no calibration necessary Type 7.6 Column thermostat Type Thermostat using Peltier technology for two intelligent separation columns Adjustable temperature range 0 - +80 °C, in 0.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.7 Eluent degasser 7.7 Eluent degasser Material Fluoropolymer Resistance to solvents No restriction (except PFC) Time to establish vacuum < 60 s 7.8 High-pressure pump Type ￭ ￭ ￭ ￭ ￭ ￭ Serial dual-piston pump Intelligent pump head recognition Chemically inert Metal-free pump heads Materials in contact with the eluent: PEEK, ZrO2, PTFE/PE Self-optimizing flow and pressure Flow rate Adjustable flow range 0.001 - 20.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Gradient capability 7 Technical specifications Isocratic or gradient (extendable to quaternary) Profile Step, linear, convex and concave Resolution < 1 nL/min 7.9 Injection valve Actuator switching time typ. 100 ms Maximum operating pressure 35 MPa (350 bar) Material PEEK 7.10 Suppressor Resistance to solvents No restriction Switching time typ. 100 ms 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.12 Metrohm CO2 Suppressor (MCS) 7.12 Metrohm CO2 Suppressor (MCS) Material Fluoropolymer Resistance to solvents No restriction (except PFC) Underpressure Working range Microprocessor controlled/stabilized Time to establish after starting < 30 s Capillary volume 400 µL Recommended flow range 0.1 - 1.0 mL/min 7.13 Detector You can find the technical specifications for the detector in the detector's manual. 7.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.16 7 Technical specifications Interfaces USB Input 1 USB upstream, type B (labeled PC for connecting to a computer) Output 2 USB downstream, type A (labeled USB 1 and USB 2) MSB 2 MSB 8-pin mini-DIN (female) (labeled MSB 1 and MSB 2 for Dosino, stirrer, remote lines, etc.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 7.18 Electromagnetic compatibility (EMC) 7.18 Emission Electromagnetic compatibility (EMC) ￭ ￭ ￭ ￭ ￭ Immunity ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ ￭ 112 ￭￭￭￭￭￭￭￭ EN/IEC 61326-1 EN 55011 / CISPR 11 EN/IEC 61000-6-3 EN/IEC 61000-3-2 EN/IEC 61000-3-3 EN/IEC 61326-1 EN/IEC 61000-6-2 EN/IEC 61000-4-2 EN/IEC 61000-4-3 EN/IEC 61000-4-4 EN/IEC 61000-4-5 EN/IEC 61000-4-6 EN/IEC 61000-4-8 EN/IEC 61000-4-11 EN/IEC 61000-4-14 EN/IEC 61000-4-28 940 Professional IC Vario ONE/SeS/PP/HPG (2.940.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 8 Warranty (guarantee) 8 Warranty (guarantee) Metrohm guarantees that the deliveries and services it provides are free of defects in materials, design or manufacturing. The general warranty period is 36 months (exclusions below) from the date of delivery, or 18 months in the event of continuous operation. The warranty remains valid on the condition that the servicing is provided by a service organization authorized by Metrohm at defined intervals and with a defined scope.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ The same warranty periods that are specified for a corresponding new part apply to parts that are replaced or repaired within the above-mentioned warranty periods. However, replacement or repair of a part does not extend the warranty period of the entire system. Deficiencies arising from circumstances that are not the responsibility of Metrohm, such as improper storage or improper use, etc., are expressly excluded from the warranty.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 9 Accessories 9 Accessories Up-to-date information on the scope of delivery and optional accessories for your instrument can be found on the Internet. NOTE When you receive your new instrument, we recommend downloading the accessories list from the Internet, printing it out and keeping it together with the manual for reference purposes.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ The Partslists webpage will be displayed. 7 Select the desired output language. 8 With the article number entered, click on the command Generate PDF. The PDF file with the accessories data will be created in the language selected. Direct access for all instruments If you are unable to find your instrument using the search as described above, this may be due to the instrument not being sold anymore.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Index A Adsorption cartridges Connection ........................ 47 Ambient conditions ................ 106 Aspiration filter (6.2821.090) .... 68 Aspiration tubing for eluent ...... 23 B Baseline Conditioning ...................... 62 Unstable ............................. 71 C Capillaries Installation ......................... 15 Carry-over ................................ 98 Cartridges Connection ........................ 47 Clean Inlet valve ...........................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index L Leak ......................................... 71 Leak sensor Installation ......................... 22 Interface ........................... 111 Technical specifications ..... 107 Leak-tightness .......................... 55 Leaky piston seals ..................... 71 Loop See also "Sample loop" ....... 29 M Mains voltage ............................. 5 Maintenance High-pressure pump ........... 71 Injection valve .................... 87 Peristaltic pump ..................
