CH-9101 Internet E-Mail Herisau/Switzerland www.metrohm.com info@metrohm.com 6.6034.033 Metrodata IC Net 2.3 SR5 8.102.0043 Software Manual 06.
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Table of contents Table of contents 1 Introduction ...............................................................1 1.1 1.2 1.3 1.4 1.5 2 Fundamentals of the operation ................................9 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 3 3.3 3.4 Fonts............................................................................................. 17 Security system ........................................................................... 17 3.2.1 Security Options window..............................
Table of contents 4.2 4.3 4.4 4.5 5 Interfaces ................................................................40 5.1 5.2 5.3 5.4 5.5 5.6 II 4.1.3 Add devices to an existing window ....................................... 30 System file handling ................................................................... 30 System functions ........................................................................ 31 4.3.1 Connect and disconnect system .......................................... 31 4.3.
Table of contents 6 Devices ....................................................................63 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 6.10 6.11 6.12 IC Net 2.3 Device installation ....................................................................... 63 6.1.1 Install devices at system creation ......................................... 63 6.1.2 Install new devices in system window................................... 65 6.1.3 Install existing devices in system window............................
Table of contents 6.13 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 IV 6.12.1 Metrohm 709 SDU features................................................. 134 6.12.2 Metrohm 709 SDU icon ....................................................... 134 6.12.3 Metrohm 709 SDU window.................................................. 134 Metrohm 818 Solvent Delivery Unit ..........................................139 6.13.1 Metrohm 818 SDU features.................................................
Table of contents 6.23 6.24 6.25 6.26 6.27 6.28 6.29 6.30 7 Methods .................................................................268 7.1 7.2 7.3 IC Net 2.3 766 IC Sample Processor.......................................................... 177 6.23.1 766 IC Sample Processor features ..................................... 177 6.23.2 766 IC Sample Processor icon............................................ 177 6.23.3 766 IC Sample Processor window ......................................
Table of contents 7.4 7.5 7.6 8 Chromatograms.....................................................316 8.1 8.2 8.3 8.4 8.5 VI 7.3.5 Export................................................................................... 278 7.3.6 Math..................................................................................... 279 Integration...................................................................................281 7.4.1 General information .....................................................
Table of contents 8.6 9 Miscellaneous functions ........................................................... 330 8.6.1 Reintegration ....................................................................... 330 8.6.2 Recalibration........................................................................ 330 8.6.3 Electronic signature............................................................. 330 8.6.4 Subtraction of a chromatogram .......................................... 332 8.6.5 Compare chromatogram.....
Table of contents 11.3 VIII Index............................................................................................363 IC Net 2.
1.1 Purpose of program 1 Introduction 1.1 Purpose of program «IC Net 2.3» is the name of the data acquisition and control software for PC-controlled ion chromatographic systems consisting of Metrohm and Bischoff instruments. The current version is a true 32-bit application. It was designed specially to operate under Windows 2000, Windows XP ans Windows Vista and uses all its benefits. The «IC Net 2.3» program can be used to create systems for recording and evaluating chromatograms.
1 Introduction 1.2 What’s new in «IC Net 2.3 SR5»? New Operating system With Service Release 5 operation of IC Net 2.3 under Windows Vista is possible. IC Net 2.3 SR5 operating under Windows Vista does not comply to 21 CFR Part 11! Autodatabase cannot be used with Windows Vista. 2 IC Net 2.
1.3 Information about the software manual 1.3 Information about the software manual This 8.102.0001 Software Manual provides a comprehensive overview of the operation of the «IC Net» program.
1 Introduction The 8.732.2013 IC Applications Collection contains all the Application Notes on the subject of ion chromatography and can be updated at any time by downloading the latest applications from the Internet under «www.metrohm.com». Last, but not least, you will find detailed information on the separating columns available from Metrohm and on special IC applications in the relevant "Application Bulletins". 1.
1.5 Installation 1.5 Installation 1.5.1 Hardware requirements for the PC 1.5.
1 Introduction To change from an IC Net installation (without 21 CFR part 11 features) to an IC Net Compliant (with 21 CFR part 11 features) installation (and back) you have to remove the previous installation! Pay attention to the corresponding dialog in the setup program. The two software packages «IC Net 2.3» and «Autodatabase 1.0» will be installed in the desired directory. Icons are created in the program folder, in the startup folder, and on the desktop.
1.5 Installation Reports Folder for storage of report files (*) and graphic files (*.wmf). Services Folder for user administration. Systems Folder for storage of system files (*.smt). For Windows Vista a special update is required to display the online help of IC Net. For licensing reasons you have to download the update file from the Microsoft web page. On the installation CD in the folder "Vista Update for HLP help" you find the link "Download WinHelp.url" to the corresponding Microsoft web page.
1 Introduction 8. Copy the Autodatabase backup files of the folders Filters, Report templates, and User database into the folders ..\IC Net 2.3\Autodatabase\Filters, ..\IC Net 2.3\Autodatabase\Report templates, and ..\IC Net 2.3\Autodatabase\User database. The folder ..\IC Net 2.3\IC Net\Data is protected against unauthorized access and manipulation, if the 21 CFR Part 11 compliance is enabled. To copy the chromatograms from the backup Data folder into the ..\IC Net 2.3\IC Net\Data folder start IC Net 2.
2.1 Starting/closing the program 2 Fundamentals of the operation 2.1 Starting/closing the program 2.1.1 Start the «IC Net» program Start the program Double-click this icon or the ICNet.exe file to start the «IC Net 2.3» program. The IC Net login window appears: Enter your User name and your Password and click on . After software installation at the first start of the software, the Add User window opens (see section 3.2.1)and a user with Administrator access level is created.
2 Fundamentals of the operation 2.2 Glossary Batch reprocessing Batch reprocessing is understood to be the subsequent reprocessing of a series of chromatograms which have been loaded in a batch reprocessing queue. During reprocessing with a selected method the settings for calibration, integration, passport, appearance and report can be altered at will (for details, see section 10).
2.2 Glossary fluenced by the integration parameters and the optionally programmable integration events which are defined in the method. In addition, the integration can be manually corrected later with the aid of the peak editor (see section 8.4), for details see section 7. Interface The «IC Net» program supports remote control for interfaces available from Metrohm and Bischoff. Interfaces generally convert analog signals to digital form, which can be handled by the computer.
2 Fundamentals of the operation 2.3 Overview of program windows «IC Net 2.3» consists of different windows whose functionality is linked together. The different windows are: 2.4 IC NET Main program window with menus for file administration, printing, method modification, options, login and user rights, window handling. CHROMATOGRAM Chromatogram window for graphic plot of running or recorded chromatograms. SYSTEM System window for control of interfaces and devices.
2.5 Icons of the main window 2.5 Icons of the main window The following icons are displayed in the IC NET main window: Open chromatogram Save chromatogram Open last batch reprocessing file Make report Print preview Send chromatograms to Autodatabase Print chromatogram Passport Method setup Integration Components Cascade all opened chromatogram windows Vertical tiling of open chromatogram windows Horizontal tiling of open chromatogram windows Appearance Enable/disable peak editor mode.
2 Fundamentals of the operation 2.6 Overview of file types The following file types are produced by the «IC Net» software: 14 *.adb Autodatabase file Contains chromatogram and method data of all determinations. *.bar Batch reprocessing file (binary file) Contains batch reprocessing data. The *.bar file is stored automatically in the Data folder. *.cal Calibration file (binary file) Contains calibration data, which can be exported with IC Net / Method / Calibration / Export calibration. The *.
2.7 Context-sensitive menus 2.7 *.rtt Report file (ASCII file) Contains a report template. The *.rtt file is stored in the program folder. *.smt System file (ASCII file) Contains the system settings. The *.smt file is stored automatically in the Systems folder. *.dev Device file (ASCII file) Contains drivers for devices. The *.dev file is stored in the Devices folder.
2 Fundamentals of the operation Shifts a chromatogram on 1/10 part of a screen downwards. [ Shift ] + [ up ] Increases a distance between channels of a chromatogram. [ Shift ] + [ down ] Reduces a distance between channels of a chromatogram. [ 0 (Zero) ] Adjusts a zero on the last point of a chromatogram (running chromatogram) or its lowest level (finished run). Only part of the chromatogram is on screen: [ Ctrl ] + [ right ] Moves one window right (without change of scale on X and Y axes).
3.1 Fonts 3 Software settings 3.1 Fonts IC NET / Options / Fonts This option allows the selection of fonts used by the system. Font for dialogs... Selection of font used for dialog boxes. Default setting: MS Sans Serif / Standard / 8 pt. Font for reports... Selection of font used for report output to the screen or printer. Default setting: Courier New / Standard / 10 pt. Font for tables... Selection of font used for data presentation in tables on the screen.
3 Software settings User Window Possible actions Novice, Master New Password This window allows the user to change his Password. Administrator Security options 3.2.1 This window allows editing the User List and the Password Options, it is also possible for an administrator user to change his Password. Security Options window IC NET / Options / Security This window contains the user administration and password options. Only a user with Administrator access level can open it.
3.2 Security system Edit current user's settings (username, full name, access level, status), opens the Modify User window. Change the password of the administrator logged in. Opens the New Password window. Add/Modify User User User name of the user. Full Name Full name of the user, this name is displayed in the status bar of the main window throughout an IC Net session. Level Access level of the user. Restricted access to program functions.
3 Software settings failed Login attempts exceeds the limit set in the Password Options. The user can be set Active again by the Administrator. Removed The User is not able to work with «IC Net», he is removed from the system but he is still listed in the User List. The user can be set Active again by the Administrator. Password Options This tab allows to specify Password and Login related security settings. Minimum Password Length Minimum number of characters for the Password. Range: 2...10, default 6.
3.3 Audit Trail Automatic Logout After After the specified period of inactivity (in minutes) the system is locked, to protect it against unauthorized access. Range: 1,...999, default 30. The program prompts for username and password every time the system starts. This username stamps methods, chromatograms and reports, created during the working session. During the work it is possible to change the current user with the menu item IC NET / Options / Lock system. 3.
3 Software settings Audit Trail window The Audit Trail window displays the Audit Trail records of the IC Net or Chromatogram History in a table. Easy access to specific information is enabled by several sort and filter options of the log window. The Log Window can be sorted according to one of the six columns by clicking the title. A star ( * ) at the end of the title indicates the sorting criterion. Refresh window, not available for Chroma- togram History.
3.4 Global settings Sort window This window offers a more refined possibility to arrange log data by combining the column titles of the Audit Trail window to a Sort Sequence where the Audit Trail records are sorted. Sort Sequence Available Sort criteria of the Sort Sequence, the sort criteria are applied in the order of their entry in the list. Unused sort criteria. Select marked sort criterion. Deselect marked sort criterion. 3.
3 Software settings If method changed Don’t ask to save Ask to save Global settings for saving of method files: The method file is not saved automatically. It can be saved only with File / Save / Method. The user is asked if the method should be saved. Overwrite without asking Method files are overwritten without confirmation. If disk method is newer Ask to overwrite Global settings for overwriting of method files: The user is asked if the method should be overwritten.
3.4 Global settings Enable Always, if you use «IC Net» together with an Online IC. In This case Method/Method setup/Export/Send data to Online has to be checked too, for details see the Online IC instructions for use. User comments Changes on methods, systems, chromatograms,… : Require Comment on changes The user has to comment a change. 21 CFR Part 11 On Switch on/off global 21 CFR Part 11 settings. Is automatically activated and cannot be altered, if IC Net Compliant is installed.
3 Software settings Opening chromatograms Global settings for opening chromatograms: Ignore last data directory If this option is enabled, the directory is opened where the current selected chromatogram is saved. If this option is disabled, the directory is opened where the last opened chromatogram is saved. 26 IC Net 2.
4.1 System creation 4 Systems The combination of Metrohm and Bischoff devices connected to an interface is called a system. The system includes all the settings of the devices, their time program, the data acquisition parameters and the processing method which have been optimized for the specific separating column and the determination to be carried out with it. A system is used to start single determinations or determinations with the help of a sample queue. Systems are stored as system files (*.
4 Systems After clicking on , the window for addition of interfaces to the toolbar appears. This step can be skipped if the desired interface has been already added to the toolbar (see section 5.1.2). If this is not the case, the interface has to be selected from the Metrohm Interfaces or Bischoff Interfaces groups.
4.1 System creation For every device to be controlled with «IC Net», open the group which contains this device, select the device and the Serial port of the interface where the device is connected to. After clicking on , the device is added to the SYSTEM window. If desired, an additional data recorder and a timer of the More modules group can be added to the system.
4 Systems Watch window Data recorder Devices 4.1.3 Add devices to an existing window SYSTEM / Setup / New devices/ Install new device Add new devices to the open SYSTEM window. For every device to be added to the system, open the group which contains this device and select the device and the serial port of the interface where the device is connected to. After clicking on , the device is added to the SYSTEM window.
4.3 System functions Save the current system file (*.smt) in the desired folder of the Systems directory. SYSTEM / System / Close Close the selected system. Delete a system To delete an existing system, delete the *.smt file in the Systems folder. 4.3 System functions 4.3.1 Connect and disconnect system To make possible manual control of the system devices and start of determinations, the selected system must be connected to the workplace.
4 Systems • Start Bischoff detectors. • Switch Metrohm Autosamplers and Bischoff Autosamplers to remote control. • Start recording of the measurement signal using the method of the connected system. Independently of the set chromatogram Duration, the measurement signal is recorded until the data acquisition is stopped if a new determination is started with Start determination or by clicking the icon of the chromatogram window. In this case the user is asked if the recorded baseline should be saved or not.
4.3 System functions If the Auto restart option is enabled or if a sample queue is active, a new determination is started immediately after the running determination is stopped. If you want to avoid this, disable the Auto restart option or Pause the sample queue. SYSTEM / Control / Stop data acquisition Stop data acquisition of the running determination immediately and save the recorded chromatogram automatically if the Save chromatogram after the run option on the Passport / Processing tab is enabled.
4 Systems Calibration level Info 1 / Info 2 Volume Dilution Vial number Amount chromatogram window and in the Chromatogram open window. Calibration level (0 = sample; 1…n = calibration solutions). Sample description. Injected volume in µL. Dilution of the sample. Autosampler vial position to take sample from. Sample amount.
4.4 System settings 4.4 System settings 4.4.1 Modify system window SYSTEM / Setup / Drag icons If this option is enabled, the device icons can be resized and moved in the SYSTEM window and the SYSTEM window itself can be resized. To resize an icon or the window, move the cursor to the desired appears. Press the left mouse button and resize object until the object to the desired size. To move an icon, move the cursor to the desired object until appears.
4 Systems The time program of the loaded object or the data acquisition (RECORDER) is started at the moment the sample is injected. Start with inject The objects can be moved from one field to the other using the or 4.4.4 buttons. Install new devices SYSTEM / Setup / New devices/ Install new device Add new devices to the open SYSTEM window.
4.5 System state window 4.4.7 Show system parameters SYSTEM / Setup / Parameters / Startup hardware Display of the name of the method linked to the system, the main measurement channel, the configuration settings for the devices, and the system startup values. SYSTEM / Setup / Parameters / Start with determination Display of the objects defined in the Start mode window. The time programs are displayed if the programs are enabled.
4 Systems 4.5.2 Running Running program or chromatogram data acquisition. Running program (xxx min left) Running time program with time display. Waiting for INJECT Waiting for "INJECT" to start program and/or chromatogram data acquisition as defined in the Start mode. INJECT done Injection valve has been switched to the "INJECT" position. Stopping Determination has been stopped. Finished Program or chromatogram data acquisition has been finished. SHUTDOWN System is shutdown.
4.5 System state window IC Net 2.3 E295 Storage of memory values failed (repeat last action; if error reappears, call Metrohm service). E296 Instrument stopped (restart instrument; if error reappears, call Metrohm service). E297 Storage of remote line values failed (repeat last action; if error reappears, call Metrohm service). E298 Storage of flow correction value failed (repeat last action; if error reappears, call Metrohm service).
5 Interfaces 5 Interfaces The «IC Net» program supports remote control for interfaces available from Metrohm and Bischoff. Interfaces generally convert analog signals to digital form, which can be handled by the computer. Interfaces are stored as device files (*.dev) in the Devices directory. All Metrohm interfaces and Metrohm devices start with Me*.dev; all Bischoff interfaces and Bischoff devices start with Bi*.dev. 5.1 Interface installation 5.1.
5.1 Interface installation Metrohm Interfaces or Bischoff Interfaces groups. After selecting the serial port where the interface is connected to and clicking on , the interface icon will appear on the toolbar (PC Boards will not appear on toolbar). Details to the other steps of the system wizard procedure (installation of devices, processing method, and data source) see section 4.1.1. 5.1.
5 Interfaces Click on to open the Adding interfaces to your workplace window and proceed as described in section 5.1.1. 5.1.3 Delete interfaces IC NET / Options / Devices setup Open the WORKPLACE window which shows all interfaces and devices installed. This window can be opened only by the user with Administrator access level. Select the desired interface and click on to delete the interface from the workplace. 5.2 762 IC Interface 5.2.
5.2 762 IC Interface 5.2.3 762 icons in the system window The 762 System 1 and 762 System 2 icons are available in the SYSTEM window if the 762 System [1] or 762 System [2] have been installed with the Install existing devices option (see section 6.1.3). By clicking this icon using the right mouse button, the following menu items can be selected: Open Open the 762 System [#] window for programming the event output lines (this window can also be opened by double-clicking the icon).
5 Interfaces Measuring per second Number of data points measured per s. Entry range: 10, 20, 30, 50, 60 points/s Range Range for AD converter. Example: converts ± 2500 mV to 224 bits Entry range: 2500, 1250, 625, 312.5, 156.25, 78.125, 39.062 mV Second press on RUN/STOP means 'stop' when measuring If this option is enabled, the running data acquisition will be stopped if the RUN/STOP button on the 762 IC Interface front panel is pressed. This is equal to Stop determination.
5.2 762 IC Interface Protected Protocol Data transfer with Protected Protocol between 830 IC Interface and PC for enhanced data security. Data transfer without Protected Protocol between 830 IC Interface and PC, select this option if there is a problem with the RS communication using the Protected Protocol. Baud Rate Parity Handshake FIFO size Baud rate of the device. Selection: 1200...115200 Default value: 115200 Parity check.
5 Interfaces Put on desktop Change 5.2.5 Possibility for setting COM port parameters (details see on-line help). Possibility for changing the COM port. The following window is opened, where the COM port can be changed by clicking on the desired entry.
5.2 762 IC Interface The Program tab contains the two following subpages: Program Main time program with all program steps. Events configuration Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. First column Time at which program instruction is applied. Entry range: 0.0 ... 999.
5 Interfaces Program END, RESET The END flag can be used to end a program, especially if the program time should be longer than the chromatogram duration. Additional steps after this flag are not allowed. The RESET flag is used to reset the parameters to the system startup values. Events configuration On the Events configuration subtab user-defined event commands can be defined, which can be inserted into a time program after being activated with .
5.3 830 IC Interface Events setup The Events setup tab of the 762 System [#] window contains the startup values for the seven event output lines of system 1 or system 2 of the 762 IC Interface. These startup values are sent each time the system is connected or a determination is started. Each event output line can be set to the following values: 0 Line off, inactive (open) 1 Line on, active (0 V) For technical information see 762 Instructions for Use. 5.3 830 IC Interface 5.3.
5 Interfaces 5.3.2 830 icon on the toolbar The 830 icon is available on the toolbar if a 830 IC Interface has been installed with the new system wizard or by using the option of the WORKPLACE window (see section 5.1.2). By clicking this icon the 830 IC Interface window for parameter settings opens. 5.3.
5.3 830 IC Interface Label Optional label to name the interface with maximum 8 characters. ROM version Instrument program version number. Measuring per second Number of data points measured per s. Entry range: 10, 20, 30, 50, 60 points/s Range Range for AD converter. Example: converts ± 2500 mV to 224 bits Entry range: 2500, 1250, 625, 312.5, 156.25, 78.125, 39.062 mV Send current parameters to 830 IC Interface.
5 Interfaces Protected Protocol Data transfer with Protected Protocol between 830 IC Interface and PC for enhanced data security. Data transfer without Protected Protocol between 830 IC Interface and PC, select this option if there is a problem with the RS communication using the Protected Protocol. Display of the baud rate of the device. Parity Display of the parity check. Handshake Display of the software handshake mode. FIFO size Display of the intermediate memory in byte.
5.3 830 IC Interface Links The Links tab of the 830 IC Interface is used for COM port selection and settings. Alias Name of the interface or device. COM # If this entry is clicked with the right mouse button, the following menu appears: Possibility for setting COM port parameters (details see on-line help). Possibility for changing the COM port. The following window is opened, where the COM port can be changed by clicking on the desired entry. Put on desktop Change IC Net 2.
5 Interfaces 5.3.5 830 Event output lines 830 System [#] icon / Open The 830 System [#] window with programs and settings for the seven event output lines is opened by selecting this menu option with the right mouse button or by double-clicking the 830 icon in the SYSTEM window. It consists of the tabs Program and Events setup. Time program On the Program tab of the 830 System [#] window a user-defined time program for the event output lines of the 830 IC Interface can be entered.
5.3 830 IC Interface Program On the Program subpage, program steps including time, program instruction and parameter can be entered. First column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Second column Program instruction (see below).
5 Interfaces Events configuration On the Events configuration subtab user-defined event commands can be defined, which can be inserted into a time program after being activated with . Name (1st column) User-definable name of the event command (e.g. Start_766). Event command (2nd column) Setting the event output lines 1…7.
5.3 830 IC Interface Events setup The Events setup tab of the 830 System [#] window contains the startup values for the seven event output lines of system 1 or system 2 of the 830 IC Interface. These startup values are sent each time the system is connected or a determination is started. Each event output line can be set to the following values: 0 Line off, inactive (open) 1 Line on, active (0 V) For technical information see 830 Instructions for Use. IC Net 2.
5 Interfaces 5.4 771 Compact Interface 5.4.1 771 Compact Interface features The Metrohm 771 Compact Interface is a two channel Analogto-Digital Converter to acquire two analog signals from two different detectors simultaneously or time independent. It contains the following parts: • Two 24-bit Analog-to-Digital Converters (often abbreviated as ADC) to acquire two analog signals from two different detectors simultaneously (for example conductivity detection and UV detection). • Two external start inputs.
5.4 771 Compact Interface Range Range for AD converter. Example: converts ± 2500 mV to 224 bits Entry range: 2500, 1250, 625, 312.5, 156.25, 78.125, 39.062 mV Read current parameters from 771 Compact Interface. Send current parameters to 771 Compact Interface. RS settings The RS settings tab of the 771 Compact Interface window contains RS232 parameters for the 771 Compact Interface. Baudrate Parity Handshake IC Net 2.3 Baud rate of the device. Selection: 1200...
5 Interfaces Read current parameters from 771 Compact Interface. Send current parameters to 771 Compact Interface. Links The Links tab of the 771 IC Interface is used for COM port selection and settings (details see section 5.2.4 Links). 5.5 Metrohm PC Board The 2.714.0310 Metrohm PC Board Interface is a two channel Analog-to-Digital Converter (often abbreviated as ADC) to acquire two analog signals from two different detectors simultaneously or time independent (e.g.
5.6 Global timer 5.6 Global timer 5.6.1 Timer icon The global timer is used for programming system tasks which are started automatically daily or once at the desired time. The Global timer icon is available on the toolbar if the Timer has been installed with the new system wizard or by using the option of the Workplace window (see section 5.1.2). By clicking this icon the Timer window for parameter settings opens. 5.6.
5 Interfaces Add a new subtask for the selected daily task. The time and program instruction for this subtask has to be entered in the opened subwindow and the system file to which this instruction should be applied must be selected in the Systems folder. Delete the selected task from the timer program. The following program instructions can be used for userdefined event program tasks in the Timer program window: Open Close Startup Start Stop Shutdown 62 Open the selected system.
6.1 Device installation 6 Devices The «IC Net» program supports remote control for Metrohm and Bischoff instruments. Each instrument within a system is a device. Devices are stored as device files (*.dev) in the Devices directory. All Metrohm devices start with Me*.dev and all Bischoff devices start with Bi*.dev. 6.1 Device installation 6.1.1 Install devices at system creation IC NET / File / New / System The New system wizard window and a new SYSTEM window are opened.
6 Devices After clicking on , the window for addition of interfaces to the toolbar appears (details see section 4.1.1). After clicking on , the window for addition of devices to the system window appears. For every device to be controlled with «IC Net», open the group which contains this device, select the device and the Serial port of the interface where the device is connected to. After clicking on , the device is added to the SYSTEM window.
6.2 Watch window 6.1.2 Install new devices in system window SYSTEM / Setup / New devices/ Install new device Add new devices to the open SYSTEM window using the New system wizard window (see above). For every device to be added to the system, open the group which contains this device and select the device and the serial port of the interface where the device is connected to. After clicking on , the device is added to the SYSTEM window.
6 Devices 6.2.2 Watch window settings Watch window icon / Open The WATCH WINDOW displays all live parameters of the interfaces and devices included in the connected system. It can be set to be opened automatically if a determination or measure baseline is started with SYSTEM / Setup / Watch window (see section 4.4.2). The WATCH WINDOW can be configured manually by clicking the frame titles inside the window with the right mouse button.
6.2 Watch window measurement value is inside the normal range. Choose color / Background / Active Set display color for field background in the SYSTEM STATE window for running tasks. Choose color / Background / Passive Set display color for field background in the SYSTEM STATE window for finished tasks. The colors set for this fields are also applied to the measurement display fields in the control tabs of some interfaces and devices. IC Net 2.
6 Devices 6.3 732 IC Detector 6.3.1 732 IC Detector features The Metrohm 732 IC Detector is a conductivity detector for ion chromatography. The associated thermostatable detector block can be used for recording of chromatograms with and without chemical suppression and is normally installed in the 733 IC Separation Center. The two following versions are available: • 2.732.0010 IC Detector with standard detector block • 2.732.
6.3 732 IC Detector 6.3.3 732 IC Detector window 732 icon / Open The 732 IC Detector window for parameter settings is opened by selecting this menu option with the right mouse button or by double-clicking the 732 icon in the SYSTEM window. It consists of the four tabs Display, Method parameter, Program, and Links. Display The Display tab of the 732 IC Detector window is only available for a connected system. It displays the current measurement values and parameters settings of the 732 IC Detector.
6 Devices Range Full scale Cell const. Thermostat Offset Damping Display of measuring range in µS/cm. Display of full scale range in µS/cm. Display of cell constant of the conductivity cell in /cm. Display of temperature within in the detector block. Display of offset of the zero point. Display of electronic damping of the analog output. Zero Triggers the auto-zero function. Switch off the auto-zero function. Update all display values.
6.3 732 IC Detector Range Measuring range in µS/cm Selection: 100, 200, 500 µS/cm and 1, 2, 5, 10 mS/cm Full scale The full scale range (operating range) sets the desired sensitivity for the analog output. The possible values of the full scale range in µS/cm depend on the preset measuring Range. Ion Combination of polarity and temperature coefficient: Anion polarity = +, temperature coeff. = 2.5 Cation polarity = –, temperature coeff. = 1.
6 Devices The Program tab contains the two following subpages: Program Remote configuration Main time program with all program steps. Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. First column Second column Third column ENABLED Will be executed by 72 Time at which program instruction is applied. Entry range: 0.0 ... 999.
6.3 732 IC Detector List of program instructions The following program instructions can be added to the time program on the Program subpage: Instruction Parameter entry Meaning Remote 0, 1, * Set remote output lines 1...8 to the desired values. For entry of the first value, enter 1, 0 or *. For entry of the other values, move the cursor in front of the value to be changed and enter 1, 0 or *. Flag reset, end Set program flag.
6 Devices Pmax 0.0 ... 25.0 MPa Set maximum pressure limit for the high-pressure pump connected to the 732 IC Detector to the desired value. Pmin 0.0 ... 25.0 MPa Set minimum pressure limit for the high-pressure pump connected to the 732 IC Detector to the desired value. Remote configuration On the Remote configuration subtab user-defined remote commands can be defined, which can be inserted into a time program after being activated with .
6.4 819 IC Detector 6.4 819 IC Detector 6.4.1 819 IC Detector features The Metrohm 819 IC Detector is a conductivity detector for ion chromatography. The associated thermostatable detector block can be used for recording of chromatograms with and without chemical suppression and is normally installed in the 820 IC Separation Center. The two following versions are available: • 2.819.0010 IC Detector with standard detector block • 2.819.
6 Devices Display The Display tab of the 819 IC Detector window is only available for a connected system. It displays the current measurement values and parameters settings of the 819 IC Detector. Conductivity abs. Cond. Ion Range Full scale Cell const. Thermostat Offset 76 Live display of measured conductivity (auto-zero value) in µS/cm. This field is available for the WATCH WINDOW.
6.4 819 IC Detector Damping Remote lines Zero Zero Display of electronic damping of the analog output. Display value of remote line settings 1…8. Triggers the auto-zero function. Switch off the auto-zero function. Method parameter The Method parameter tab of the 819 IC Detector window contains the method startup values for the instrument. These values are sent and applied to the 819 IC Detector by Start determination and Startup hardware, or if the values are sent manually with .
6 Devices Custom independent selection of polarity and temperature coeff. Polarity Temperature coeff. Cell constant Thermostat Offset Damping Remote lines Polarity of the analog output signal: + positive polarity (e.g. for anions) – negative polarity (e.g. for cations) Converts automatically the conductivity from the operating temperature of the measuring cell to the reference temperature of 20°C. Selection: 1.5, 2.5 %/°C Cell constant of the conductivity cell in /cm. Entry range: 13 ...
6.4 819 IC Detector The Program tab contains the two following subpages: Program Remote configuration Main time program with all program steps. Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. First column Second column Third column ENABLED IC Net 2.3 Time at which program instruction is applied. Entry range: 0.0 ... 999.
6 Devices Add new program instruction. Delete selected program instruction. Test the time program (error messages are displayed if program is wrong). List of program instructions The following program instructions can be added to the time program on the Program subpage: Instruction Parameter entry Meaning Remote 0, 1, * Set remote output lines 1...8 to the desired values. For entry of the first value, enter 1, 0 or *.
6.4 819 IC Detector Remote configuration On the Remote configuration subtab user-defined remote commands can be defined, which can be inserted into a time program after being activated with . Name (1st column) User-definable name of the remote command (e.g. Start_766). Remote command (2nd column) Setting the remote output lines 1…8. 0 (line off, inactive, open) 1 (line on, active, 0 V) * (leave line in current status) For entry of the first value, enter 1, 0 or *.
6 Devices 6.5 761 Compact IC 6.5.1 761 Compact IC features The Metrohm 761 Compact IC is a PC-controlled system for ion chromatographic analyses. The two following versions are available: • 2.761.0010 Compact IC without suppressor module • 2.761.0020 Compact IC with suppressor module All the parts of the 761 Compact IC like injection valve, highpressure pump, conductivity detector, suppressor module and peristaltic pump, are normally fully controlled by the «761 Compact IC» program.
6.5 761 Compact IC If the system is connected and the 761 icon is clicked with the right mouse button, the following menu appears: Open Open the 761 Compact IC window for parameter settings (this window can also be opened by double-clicking the icon). 6.5.3 Hardware Open the hardware settings window. Diagnostics Open the diagnostics window (for service personal only). Unlink Delete the 761 icon from the system.
6 Devices Conductivity, µS/cm Live display of measured conductivity. Pressure, MPa Live display of measured pressure. The color settings for this two fields can be changed by clicking the fields with the right mouse button (see section 6.2.2). Display of actual values. Flow, mL/min Display of current flow rate of the highpressure pump. Pressure max, MPa Display of current maximum pressure limit for high-pressure pump. Pressure min, MPa Display of current minimum pressure limit for high-pressure pump.
6.5 761 Compact IC Full scale, µS/cm Remote lines even without connection to the PC. Entry range: 0.0 ... 25.0 MPa Startup value for full scale range. Selection: 50, 250, 1000 µS/cm Startup value for remote line settings 1…8. Selection: 0, 1 Initial The Initial tab of the 761 Compact IC window for settings of system startup values is only available for a disconnected system.
6 Devices The Program tab contains the two following subpages: Program Main time program with all program steps. Remote configuration Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. 86 First column Time at which program instruction is applied. Entry range: 0.0 ... 999.
6.5 761 Compact IC List of program instructions The following program instructions can be added to the time program on the Program subpage: Instruction Parameter entry Meaning Valve Inject, Fill Switch injection valve to "INJECT" or "FILL" position. FullScale 50, 250, 1000 µS/cm Set full scale range to the selected value. ICPump on, off Switch on or off the high-pressure pump. Flow 0.2 ... 2.5 mL/min Set flow rate of the high-pressure pump to the desired value. Pmax 0.0 ... 25.
6 Devices Remote configuration On the Remote configuration subtab user-defined remote commands can be defined, which can be inserted into a time program after being activated with . Name (1st column) User-definable name of the remote command (e.g. Start_766). Remote command (2nd column) Setting the remote output lines 1…8. 0 (line off, inactive, open) 1 (line on, active, 0 V) p (pulse) * (leave line in current status) For entry of the first value, enter 1, 0, p or *.
6.5 761 Compact IC 761 unit version Selection of instrument version: 761.0010 = 761 Compact IC without suppressor 761.0020 = 761 Compact IC with suppressor Suppressor Suppressor module: Automatic switching to the next position each time the injection valve is switched either to the Fill or to the Inject position. Autostep with Use peristaltic pump If this option is disabled, the peristaltic pump is not switched on automatically at the start of a determination or with Startup hardware.
6 Devices 6.5.4 Hardware settings Selection of the Hardware menu item of the 761 icon using the right mouse button opens the hardware settings window consisting of the three tabs Hardware, Outputs and Alarm stops. Hardware The Hardware tab of the hardware settings window defines general parameters which are set automatically at power on of the 761 Compact IC. Remote lines after power on The remote output lines 1…8 are set to this values after power on or a manual stop with Shutdown hardware.
6.5 761 Compact IC Cell constant Cell constant of the conductivity cell for correct display of the absolute conductivity. Enter the cell constant printed on the detector block into this field. Range: 0.1 ... 99.9 /cm For a precise determination of the cell constant, pump a calibration solution of known conductivity through the IC system, observe the displayed conductivity and change the cell constant until the correct conductivity value is displayed.
6 Devices Pump stop (P max/min) Pump stopped because pressure limits are exceeded. Hardware error Hardware error detected at the 761 Compact IC (high-pressure pump, injection valve, or suppressor not working correctly). Alarm leak detector Leak detector has detected solvent in the instruments interior. Remote line Set remote output lines 1…8.
6.5 761 Compact IC Event Pump stop Hardware error Leak Power on IC Net 2.3 Events for alarm stop: Pump stopped because pressure limits are exceeded. Hardware error detected at the 761 Compact IC (high-pressure pump, injection valve, or suppressor not working correctly). Leak detector has detected solvent in the instruments interior. This information is also stored in the instrument itself, so that it is stopped automatically even without connection to the PC.
6 Devices 6.6 817 Bioscan 6.6.1 817 Bioscan features The Metrohm 817 Bioscan is a pulsed amperometric detector for use with a modular IC system. The instrument is designed to carry out determination of carbohydrates but is also capable of analyzing alcohols (polyalcohols), sugar alcohols, as well as other oxidizable substances. The 817 Bioscan is fully operated using the «IC Net» program. It is normally connected directly to a PC COM port using the 6.2134.080 or 6.2134.090 cable and the 6.2125.
6.6 817 Bioscan Mode DC Pulse Scan Cell Oven Temperature Control Selection of detector mode: In DC mode, a constant potential is applied to the working electrode, the analytes are oxidized or reduced in accordance with their electrochemical properties. The Pulse mode works with three different working potentials, which are applied cyclically.
6 Devices Valve Analog output Actual Mode Cell Oven Control Manual switching of the injection valve at the 812 Valve Unit: Switch injection valve to "Fill" position (control line Cont1 is set to 0). Switch injection valve to "Inject" position (control line Cont1 is set to 1). Manual setting of the analog output signal to zero. Trigger the autozero function. Display of actual values. Display of current detector mode. Display of current cell state. Display of current ovent state.
6.6 817 Bioscan Method data Cell potential Oven Range Full scale Filter Offset Electric potential applied to the amperometric detector cell in V Range: -2 ... +2 V. Switching on/off column oven and setting operating temperature. Range: 15 ... 60 °C Selection of measuring range: pA 10 ... 5000 pA. nA 0.1 ... 50 nA. µA 0.01 ... 5 µA. The full scale range (operating range) sets the desired sensitivity for the analog output. The possible values of the full scale range depend on the preset measuring Range.
6 Devices E1, E2, E3 t1, t2, t3 Sample time Oven Range Full scale Offset 98 Working potentials. Range: -2 ... +2 V. Time duration to apply the working potential. Range: 0.01 ... 2 s Measuring time at the end of working potential E1. Selection: 20, 40, 60, 80, 100 ms Switching on/off column oven and setting operating temperature. Range: 15 ... 60 °C Selection of measuring range: pA 10 ... 5000 pA. nA 0.1 ... 50 nA. µA 0.01 ... 5 µA.
6.6 817 Bioscan Actual E1, E2, E3 t1, t2, t3 Sample time Oven Range Full scale Offset Cycle potentials Method Actual Display of current working potentials in V. Display of current time durations for working potentials in s. Display of current sample time in ms. Display of current oven state. Display of current measuring range. Display of current full scale range. Display of current offset. Graphical display of cycle potentials. Display of working potentials set in the Method data frame.
6 Devices Range Selection of measuring range: pA 10 ... 5000 pA. nA 0.1 ... 50 nA. µA 0.01 ... 5 µA. Full scale The full scale range (operating range) sets the desired sensitivity for the analog output. The possible values of the full scale range depend on the preset measuring Range. Offset of the zero point for the analog output signal. Selection: -50 ...
6.6 817 Bioscan Send current parameters immediately to the 817 Bioscan. Parameters are not stored in the system file (*.smt) as long as the file is not saved. Operations Caption State Possibility to enter a title which is written on top of the scan mode graphics window. The current date and time are added to the title defined in the Caption field. Display of current state. Start scan mode sweep. Stop scan mode sweep.
6 Devices 6.7 871 Advanced Bioscan 6.7.1 871 Advanced Bioscan features The Metrohm 871 Advanced Bioscan is a pulsed amperometric detector for use with a modular IC system. The instrument is designed to carry out determination of carbohydrates but is also capable of analyzing alcohols (polyalcohols), sugar alcohols, as well as other oxidizable substances. The 871 Advanced Bioscan is fully operated using the «IC Net» program.
6.7 871 Advanced Bioscan opened by selecting this menu option with the right mouse button or by double-clicking the 871 icon in the SYSTEM window. It consists of the four tabs Manual, Method parameters, Program, and Links. Manual This tab of the 871 Advanced Bioscan window allows manual control of the 871 Advanced Bioscan for a connected system. It displays the following current measurement values and parameter settings of the 871 Advanced Bioscan: Mode DC Pulse IC Net 2.
6 Devices Scan Cell Oven Temperature Control The Scan mode allows potential/current curves to be recorded. Manual control of amperometric detector cell: Switch on detector cell. Switch off detector cell. Manual control of column oven (heater): Switch on oven. Switch off oven. Temperature of column oven. Range: 15 ... 45 °C Manual setting of 4 control lines. Set control lines to selected states. Aux1, Aux2, Cont1, Cont2 Setting of 4 control lines.
6.7 871 Advanced Bioscan Mode DC Pulse Scan Oven Temperature Selection of detector mode: In DC mode, a constant potential is applied to the working electrode, the analytes are oxidized or reduced in accordance with their electrochemical properties. The Pulse mode works with three different working potentials, which are applied cyclically. This frees the electrode surface from any adhering reaction products after each sweep and an activated surface is produced for the next measurement.
6 Devices Actual Mode Oven Control Display of actual values. Display of current detector mode. Display of current ovent state. Display of current control line settings. Send current parameters immediately to the 871 Advanced Bioscan. Parameters are not stored in the system file (*.smt) as long as the file is not saved. DC mode This subtab of the Method parameters tab in the 871 Advanced Bioscan window contains the method parameters for the DC mode.
6.7 871 Advanced Bioscan Startup values E Range Filter Offset Data rate Polarity Electric potential applied to the amperometric detector cell in V Range: -2 ... +2 V. Selection of measuring range: pA 10 ... 500 pA. nA 1 ... 500 nA. µA 1 ... 200 µA. Electronic damping of the analog output signal. Selection: off, 0.2, 0.5, 1, 2, 5 s Offset of the zero point for the analog output signal. Selection: -50 ... +50 % (in steps of 10 %) Setting of the data acquisition rate.
6 Devices tsample Range Offset Data rate Polarity Measuring time at the end of working potential E1. Selection: 20 … (t1 - 60) ms (in steps of 20) Selection of measuring range: nA 10 ... 500 nA. µA 1 ... 200 µA. Offset of the zero point for the analog output signal. Selection: -50 ... +50 % (in steps of 10 %) Setting of the data acquisition rate. Selection: -1, 2, 5, 10 Hz Set sign: + or - Actual E1, E2, E3 t1, t2, t3 tsample Range Filter 108 Display of current working potentials in V.
6.7 871 Advanced Bioscan Offset Data rate Polarity Cycle potentials Method Actual Display of current offset. Display of current data rate. Display of current polarity. Graphical display of cycle potentials. Display of working potentials set in the Method data frame. Display of current working potentials. Send current parameters immediately to the 871 Advanced Bioscan. Parameters are not stored in the system file (*.smt) as long as the file is not saved.
6 Devices E1 E2 Rate Cycle mode Range Offset Data rate Start potential for sweep. Range: -2 ... +2 V. End potential for sweep. Range: -2 ... +2 V. Definition of scan rate in mV/s. Selection: 1, 2, 5, 10, 20, 50 mV/s Definition of scan cycle: full double scan E1 ... E2 ... E1 half single scan E1 ... E2 Selection: full, half Selection of measuring range: nA 10 ... 500 nA. µA 1 ... 200 µA. Offset of the zero point for the analog output signal. Selection: -50 ...
6.7 871 Advanced Bioscan Scan shell Clear display. Closes the scan file without saving. Load voltammogram. To open a saved voltammogram file (.sca). Save voltammogram. Saves the currently displayed voltammogram(s) as a voltammogram file (.sca). Export voltammogram. Saves the currently displayed voltammogram(s) as a txt.file. Print voltammogram. Prints the currently displayed voltammogram(s). Copy to clipboard. Copies the currently displayed voltammogram(s) to the clipboard.
6 Devices Stop voltammogram acquisition. Open Help file. Links The Links tab of the 871 Advanced Bioscan is used for COM port selection and settings (details see section 5.2.4 Links). 112 IC Net 2.
6.8 733 IC Separation Center 6.8 733 IC Separation Center 6.8.1 733 IC Separation Center features The Metrohm 733 IC Separation Center is a thermally and electronically isolated wet part which accommodates injectors, columns, detectors, suppressor module and pulsation dampener and is controlled by and connected to the 732 IC Detector or the 762 IC Interface. The following versions are available: • 2.733.0010 IC Separation Center with 1 injector • 2.733.
6 Devices with the 733 IC Separation Center, the 732 IC Detector and the 762 IC Interface can be found in the 762 Instructions for Use. 6.8.2 733 IC Separation Center icon The 733 icon is available in the SYSTEM window if a 733 IC Separation Center has been installed with the New system wizard or by using the Setup/New devices/Install new device option of the SYSTEM window (see section 6.1.2).
6.8 733 IC Separation Center 733 IC Separation Center connected to 732 Configuration valve A valve A + valve B 733.0130 Separation Center connected to 762 Display of current instrument configuration.* 733 IC Separation Center with 1 injector. 733 IC Separation Center with 2 injectors. valve A + suppressor 733 IC Separation Center with 1 injector and 1 suppressor module. Valve A fill/inject Display of current valve position.* Switch valve A to "fill" position.
6 Devices Set automatic switching of the suppressor module after each "fill" or "inject" of valve A. Selection: fill, inject, off * only available for 733 IC Separation Center connected to 732 Entry field Time program The Program tab is only available for a 733.0XX0 Separation Center connected to a 762 IC Interface. The 733 IC Separation Center that is connected to a 732 IC Detector is controlled by the time program of the detector. On the Program tab of the 733.
6.8 733 IC Separation Center Add new program instruction. Delete selected program instruction. Test the time program (error messages are displayed if program is wrong). List of program instructions The following program instructions can be added to the time program on the Program page: Instruction Parameter entry Meaning ValveA fill, inject Switch injection valve A to “fill” or “inject” position. ValveB fill, inject Switch injection valve B to “fill” or “inject” position.
6 Devices 6.9 820 IC Separation Center 6.9.1 820 IC Separation Center features The Metrohm 820 IC Separation Center is a thermally and electronically isolated wet part which accommodates injectors, columns, detectors, suppressor module, column heater and pulsation dampener. It is controlled by and connected to the 819 IC Detector or the 830 IC Interface. The following versions are available: • 2.820.0210 IC Separation Center with 1 injector and column heater, metal-free • 2.820.
6.9 820 IC Separation Center stalled Separation Center, if two detectors are connected to a 820 IC Separation Center with two injection valves. If the Separation Center is connected to the 830 IC Interface, one 6.2115.070 cable for every event line is needed. The number depends on the type of Separation Center, e.g. for the 820.0X30 IC Separation Center three event lines are necessary to control the valve (fill, inject) and the suppressor (step). If the Separation Center is equipped with a column heater (2.
6 Devices 6.9.3 820 IC Separation Center window 820 icon / Open The 820 IC Separation Center window for parameter settings is opened by selecting this menu option with the right mouse button or by double-clicking the 820 icon in the SYSTEM window. It consists of the three tabs Manual, Program, Thermostat and Links. The title bar of the window displays 820 IC Separation Center, (if connected to IC Detector) or 820.
6.9 820 IC Separation Center Valve A fill/inject Display of current valve position.* Switch valve A to "fill" position. Switch valve A to "inject" position. Valve B fill/inject Display of current valve position.* Switch valve B to "fill" position. Switch valve B to "inject" position. Suppressor in position Display of current suppressor status.* Switch suppressor module to next position. * fill, inject, off Display of current auto step status.
6 Devices First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6.9 820 IC Separation Center Thermostat The Thermostat tab of the 820 IC Separation Center or 820.02X0 IC Separation Center window contains the temperature control of the column heater. Only available for a 2.820.02X0 Separation Center that is connected either to the 819 IC Detector and installed as 820.02X0 to IC Detector, or to the 830 IC Interface and installed as 820.02X0 to Interface. Actual temperature Set point IC Net 2.
6 Devices Start with startup hardware Enabled Start the Thermostat with the startup of the system hardware. The temperature of the column heater is monitored by «IC Net», an injection is triggered, if the set operating temperature of the column heater is reached. The operating temperature is reached, when the actual temperature is within ± 1 °C of the temperature set above. Disabled The temperature of the column heater is not monitored by «IC Net».
6.10 709 IC Pump 6.10 709 IC Pump 6.10.1 709 IC Pump features The Metrohm 709 IC Pump is a serial dual piston high pressure pump for ion chromatography. The two following versions are available: • 2.709.0010 IC Pump (standard version) • 2.709.0110 IC Pump (metal-free version) The 709 IC Pump, which is normally operated using the keypad of the instrument or via 732 IC Detector, can be fully operated using the «IC Net» program. It can be connected either to the 732 IC Detector using the 6.2125.
6 Devices 6.10.3 709 IC Pump window 709 icon / Open The 709 IC Pump window for parameter settings and manual con- trol is opened by selecting this menu option with the right mouse button or by double-clicking the 709 icon in the SYSTEM window. It consists of the three tabs Settings, Links and Advanced. Settings The Settings tab of the 709 IC Pump window is only available for a connected system. Pressure Flow 126 Live display of current pressure in MPa.
6.10 709 IC Pump value during operation, it is switched off immediately. At the same time, the Pressure field color in the 709 settings window and in the WATCH WINDOW is changed to the value set for Out of range. The set minimum limit value should lie far enough below the particular operating pressure.
6 Devices Correction factor Flow correction factor to adjust the displayed flow to the actual flow measured with the aid of a measuring cylinder. Entry range: 0.90 ... 1.10 Read current Correction factor value. Send current Correction factor value to the 709 IC Pump. This parameter is not stored in the system file (*.smt) as long as the file is not saved. 128 IC Net 2.
6.11 818 IC Pump 6.11 818 IC Pump 6.11.1 818 IC Pump features The Metrohm 818 IC Pump is a metal-free serial dual piston high pressure pump for ion chromatography. The 818 IC Pump is completely remote controlled and can be operated using the «IC Net» program. The 818 IC Pump can be connected to the 830 IC Interface using the 6.2134.080 or 6.2134.090 cable, for direct connection of the 818 IC Pump to a PC COM port, use the 6.2134.040 or 6.2134.100 cable.
6 Devices 6.11.3 818 IC Pump window 818 icon / Open The 818 IC Pump window for parameter settings and manual con- trol is opened by selecting this menu option with the right mouse button or by double-clicking the 818 icon in the SYSTEM window. It consists of the three tabs Settings, Program, Links and Advanced. Settings The Settings tab of the 818 IC Pump window is only available for a connected system. Pressure Flow 130 Live display of current pressure in MPa.
6.11 818 IC Pump used. If the pump exceeds the preset limit value during operation, it is switched off immediately. At the same time, the Pressure field color in the 818 settings window and in the WATCH WINDOW is changed to the value set for Out of range. The set minimum limit value should lie far enough below the particular operating pressure.
6 Devices First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6.11 818 IC Pump Pmax 0.0 ... 30.0 MPa Set maximum pressure limit for the high pressure pump to the desired value. Pmin 0.0 ... 30.0 MPa Set minimum pressure limit for the high pressure pump to the desired value. Links The Links tab of the 818 IC Pump is used for COM port selection and settings (details see section 5.2.4 Links). Advanced The Advanced tab of the 818 IC Pump window is only available for a connected system. IC Net 2.
6 Devices 6.12 Metrohm 709 Solvent Delivery Unit 6.12.1 Metrohm 709 SDU features A Metrohm 709 SDU (Solvent Delivery Unit) system can be used for high-pressure gradient mixture of one to four eluents. For each eluent, a 709 IC Pump is used. Each 709 IC Pump must be connected to the 762 IC Interface using a 6.2134.080 or 6.2134.090 cable. Examples for interconnections with the 709 IC Pump can be found in the 762 Instructions for Use.
6.12 Metrohm 709 Solvent Delivery Unit Manual The Manual tab of the Pump Control Unit window is only available for a connected system. Pressure Flow New flow A new flow can be set by moving the scroll bar, by entering a value or by changing the value using the up and down arrows. Entry range: 0.01 ... 20.00 mL/min (depending on number of pumps) Min/Max pressure The minimum and maximum pressure limit for the individual pump units can be set in steps of 0.1 MPa. Entry range: 0.0 ... 50.0 MPa.
6 Devices Stop after Stop time for all pump drives. Entry range: 0 ... 99999 min Send current parameters immediately to the pump control system. Parameters are not stored in the system file (*.smt) as long as the file is not saved. Start all pump drives. Stop all pump drives. Program The Program tab of the Pump Control Unit window contains a time program for the pump control system to define eluent gradients. Flow Overall flow of pump control system in mL/min.
6.12 Metrohm 709 Solvent Delivery Unit Program table Number Time Flow %B %C %D Enabled Enable time program start (a disabled program is not started). Show flow column Show Flow column in the program table. Add a new line to the time program. Delete the selected line from the time program. Start all pump drives. IC Net 2.3 Row number in the program table (readonly). Time at which the flow and/or gradient is changed. Entry range: 0.0 ...
6 Devices Interfaces The Interfaces tab of the Pump Control Unit window is only available for a connected system. Number of eluents Number of eluents used for gradient mixing. Pumps List of used pumps for gradient mixing. Clicking on , , , or opens the 709 IC Pump window for parameter settings and manual control of the selected pump. Pressure checking Selection of the pump whose pressure is checked using the Min/Max pressure limits.
6.13 Metrohm 818 Solvent Delivery Unit 6.13 Metrohm 818 Solvent Delivery Unit 6.13.1 Metrohm 818 SDU features A Metrohm 818 SDU (Solvent Delivery Unit) system can be used for high-pressure gradient mixture of one to four eluents. For each eluent, a 818 IC Pump is used. Each 818 IC Pump must be connected to the 830 IC Interface using a 6.2134.080 or 6.2134.090 cable. Examples for interconnections with the 818 IC Pump can be found in the 830 Instructions for Use.
6 Devices The Manual tab of the Pump Control Unit window is only available for a connected system. Pressure Flow New flow A new flow can be set by moving the scroll bar, by entering a value or by changing the value using the up and down arrows. Entry range: 0.01 ... 20.00 mL/min (depending on number of pumps) Min/Max pressure The minimum and maximum pressure limit for the individual pump units can be set in steps of 0.1 MPa. Entry range: 0.0 ... 50.0 MPa.
6.13 Metrohm 818 Solvent Delivery Unit Stop after Stop time for all pump drives. Entry range: 0 ... 99999 min Send current parameters immediately to the pump control system. Parameters are not stored in the system file (*.smt) as long as the file is not saved. Start all pump drives. Stop all pump drives. Program The Program tab of the Pump Control Unit window contains a time program for the pump control system to define eluent gradients.
6 Devices Flow %B %C %D Overall flow of pump control system in mL/min. Entry range: 0.01 ... 20.00 mL/min (depending on number of pumps) Individual flow rate for pump B in percent of total flow. Entry range: 0 ... 100 % Individual flow rate for pump C in percent of total flow. Entry range: 0 ... 100 % Individual flow rate for pump D in percent of total flow. Entry range: 0 ... 100 % Enabled Enable time program start (a disabled program is not started).
6.13 Metrohm 818 Solvent Delivery Unit Pumps List of used pumps for gradient mixing. Clicking on , , , or opens the 818 IC Pump window for parameter settings and manual control of the selected pump. Pressure checking Selection of the pump whose pressure is checked using the Min/Max pressure limits. Time reaction for press. min/max overflow Time delay for pump stopping at Min/Max pressure limits.
6 Devices 6.13 6.13.1 752 Pump Unit 752 Pump Unit features The Metrohm 752 Pump Unit is a 2-channel peristaltic pump which is normally used for operating the suppressor module in the 733 IC Separation Center by supplying regeneration and rinsing solutions. The 752 Pump Unit, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6.13 752 Pump Unit Manual The Manual tab of the 752 Pump Unit window for manual operation is only available for a connected system. Pump Start pump drive. Stop pump drive. Start pump with startup hardware Automatic start of pump drive with Startup hardware. Time program On the Program tab of the 752 Pump Unit window a user-defined time program can be entered. This program is started automatically as defined in the Start mode window (see section 4.4.
6 Devices ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. Test the time program (error messages are displayed if program is wrong). List of program instructions The following program instructions can be added to the time program on the Program page: Instruction Parameter entry Meaning Program RESET, END Set program flag.
6.14 753 Suppressor Module 6.14 6.14.1 753 Suppressor Module 753 Suppressor Module features The Metrohm 753 Suppressor Module consists of a control unit with a 2-channel peristaltic pump and a block containing a suppressor module. The 753 Suppressor Module, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6 Devices Manual The Manual tab of the 753 Suppressor Module window for manual operation is only available for a connected system. Pump Start pump drive. Stop pump drive. Suppressor Rotate suppressor to the next position. The time since the last step action is displayed in the field beside the button. Start pump with startup hardware Automatic start of pump drive with Startup hardware.
6.14 753 Suppressor Module First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6 Devices 6.15 6.15.1 754 Dialysis Unit 754 Dialysis Unit features The Metrohm 754 Dialysis Unit is a unit for on-line sample dialysis before sample injection. It consists of a 2-channel peristaltic pump and a dialysis cell. The 754 Dialysis Unit, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6.15 754 Dialysis Unit Manual The Manual tab of the 754 Dialysis Unit window for manual operation is only available for a connected system. Pump Start pump drive. Stop pump drive. Start pump with startup hardware Automatic start of pump drive with Startup hardware. Time program On the Program tab of the 754 Dialysis Unit window a user-defined time program can be entered. This program is started automatically as defined in the Start mode window (see section 4.4.
6 Devices Third column Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. Test the time program (error messages are displayed if program is wrong). List of program instructions The following program instructions can be added to the time program on the Program page: Instruction Parameter entry Meaning Program RESET, END Set program flag.
6.16 793 Sample Prep Module 6.16 6.16.1 793 Sample Prep Module 793 Sample Prep Module features The Metrohm 793 Sample Prep Module consists of a control unit with a 2-channel peristaltic pump and a block containing the sample preparation module. The 793 Sample Prep Module, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6 Devices Manual The Manual tab of the 793 Sample Prep Module window for manual operation is only available for a connected system. Pump Start pump drive. Stop pump drive. Actuator Rotate sample preparation module to the next position. The time since the last step action is displayed in the field beside the button. Start pump with startup hardware Automatic start of pump drive with Startup hardware.
6.16 793 Sample Prep Module First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6 Devices 6.17 6.17.1 812 Valve Unit 812 Valve Unit features The Metrohm 812 Valve Unit is a control unit with one or two built-in injection valves. The following versions are available: • 2.812.0010 IC Valve Unit with 1 injector • 2.812.0020 IC Valve Unit with 2 injectors The 812 Valve Unit, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6.17 812 Valve Unit 6.17.3 812 Valve Unit window 812 icon / Open The 812 Valve Unit window for parameter settings is opened by se- lecting this menu option with the right mouse button or by doubleclicking the 812 icon in the SYSTEM window. It consists of the three tabs Manual, Program, and Links. Manual The Manual tab of the 812 Valve Unit window for manual operation is only available for a connected system. Valve A Switch valve A to "FILL" position. Switch valve A to "INJECT" position.
6 Devices First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6.18 816 IC Eluent Selector 6.18 6.18.1 816 IC Eluent Selector 816 IC Eluent Selector features The Metrohm 816 IC Eluent Selector is a step-gradient 3-port selector, which can select one of three solutions (eluents). The 816 IC Eluent Selector, which is normally operated manually using the keypad of the instrument, can be fully operated using the «IC Net» program. The 816 IC Eluent Selector is normally connected to the 762 IC Interface using the 6.2134.080 or 6.2134.090 cable and the 6.2125.150 Adapter.
6 Devices Manual The Manual tab of the 816 IC Eluent Selector window for manual operation is only available for a connected system. Current eluent Selection of Eluent A, Eluent B, or Eluent C. Switch valves to feed the selected eluent. Flow A Possibility to rename Eluent A. Possibility to rename Eluent B. Possibility to rename Eluent C. Aliases Flow B Flow C Time program On the Program tab of the 816 IC Eluent Selector window a userdefined time program can be entered.
6.18 816 IC Eluent Selector First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). Enabled Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6 Devices 6.19 6.19.1 828 IC Dual Suppressor 828 IC Dual Suppressor features The 828 IC Dual Suppressor is a continuous, regeneration-free solid phase suppressor which is used to increase the sensitivity of the detection of anions in ion chromatography. The 828 IC Dual Suppressor, which is normally operated manually using the switches of the instrument, can be fully operated using the «IC Net» program. The 828 IC Dual Suppressor is normally connected to the 762 IC Interface using the 6.2128.180 cable.
6.19 828 IC Dual Suppressor Manual The Manual tab of the 828 IC Dual Suppressor window for manual operation is only available for a connected system. Suppressor Switch the Suppressor on. Switch the Suppressor off. Start with startup hardware Start the Suppressor with the startup of the system hardware. Time program On the Program tab of the 828 IC Dual Suppressor window a userdefined time program can be entered.
6 Devices First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). Enabled Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6.20 833 IC Liquid Handling Unit 6.20 6.20.1 833 IC Liquid Handling Unit 833 IC Liquid Handling Unit features The Metrohm 833 Liquid Handling Unit consists of a control unit with a 2-channel peristaltic pump and depending on the version of different additional modules. The peristaltic pump and one additional module can be remote controlled. The following versions are available: • 2.833.0010 IC Liquid Handling Pump Unit with peristaltic pump • 2.833.
6 Devices 6.20.3 Open Open the 833 Liquid Handling Unit window for parameter settings (this window can also be opened by double-clicking the icon). Unlink Delete the 833 icon from the system. 833 IC Liquid Handling Unit window 833 icon / Open The 833 IC Liquid Handling Unit window for parameter settings is opened by selecting this menu option with the right mouse button or by double-clicking the 833 icon in the SYSTEM window. It consists of the three tabs Manual, Program, and Links.
6.20 833 IC Liquid Handling Unit Suppressor Actuator (available only for 833 IC Suppressor Unit) Rotate suppressor to the next position. The time since the last step action is displayed in the field beside the button. (available only for 833 IC Ultra Filtration Unit) Rotate sample preparation module to the next position. The time since the last step action is displayed in the field beside the button.
6 Devices Second column Third column If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. Test the time program (error messages are displayed if program is wrong).
6.21 837 IC Degasser 6.21 6.21.1 837 IC Degasser 837 IC Degasser features The 837 IC Degasser is an instrument for continuously degassing sample or eluent flows. Gas bubbles, excess carbon dioxide or nitrogen are reliably removed. Three versions are available: • 2.837.0010 IC Eluent Degasser for two eluent flows • 2.837.0020 IC Sample Degasser for one sample flow • 2.837.0030 IC Combi Degasser for one sample flow and two eluent flows The 837 IC Degasser can be fully operated using the «IC Net» program.
6 Devices 6.21.3 837 IC Degasser window 828 icon / Open The 837 IC Degasser window for parameter settings is opened by selecting this menu option with the right mouse button or by double-clicking the 837 icon in the SYSTEM window. It consists of the four tabs Manual, Program, and Links.
6.21 837 IC Degasser Time program On the Program tab of the 837 IC Degasser window a user-defined time program can be entered. This program is started automatically as defined in the Start mode window (see 4.4.3) either at the moment the determination is started (Start with determination) or at the moment the sample is injected (Start with inject). First column Second column Third column Enabled Enable program start (a disabled program is not started). Add new program instruction.
6 Devices List of program instructions The following program instructions can be added to the time program on the Program page: Instruction Parameter entry Meaning Degasser on, off Switch the Degasser on/off. Links The Links tab of the 833 IC Degasser is used for Event line selection. 172 IC Net 2.
6.22 750 Autosampler 6.22 6.22.1 750 Autosampler 750 Autosampler features The Metrohm 750 Autosampler is a sampler for automatic sample injection for ion chromatography. It has a capacity of up to 128 samples each with an effective volume of ca. 700 µL. The 750 Autosampler, which is normally operated using the keypad of the instrument, can be fully operated using the «IC Net» program. The 750 Autosampler is normally connected to the 762 IC Interface using the 6.2134.
6 Devices 6.22.3 750 Autosampler window 750 icon / Open The 750 Autosampler window for parameter settings is opened by selecting this menu option with the right mouse button or by double-clicking the 750 icon in the SYSTEM window. It consists of the four tabs Manual, Injection, Diagnostics and Links. Manual The Manual tab of the 750 Autosampler window for manual operation is only available for a connected system.
6.22 750 Autosampler Loop size is Size of the sample loop at the 733 IC Separation Center. If the loop size is > 20 µL, only 3 injections per sample vial are possible. Move transfer needle to the desired vial position (1...128 or Home). Lower transfer needle to the desired position (1...4). Start manual rinsing using the desired vial (1...128).
6 Devices Manual injection Sample vial number
6.23 766 IC Sample Processor 6.23 6.23.1 766 IC Sample Processor 766 IC Sample Processor features The Metrohm 766 IC Sample Processor is a sampler for automatic sample injection for ion chromatography. It has a capacity of up to 127 samples each with an effective volume of up to 11 mL. The 766 IC Sample Processor, which is normally operated using the keypad of the instrument, can be fully operated using the «IC Net» program.
6 Devices If the system is connected and the 766 icon is clicked with the right mouse button, the following menu appears: 6.23.3 Open Open the 766 IC Sample Processor window for parameter settings (this window can also be opened by double-clicking the icon). Timeout Open a window for setting the timeout time (in s) for the scan command. Unlink Delete the 766 icon from the system.
6.23 766 IC Sample Processor Position Stop moving the rack to place the specified vessel at the needle. Selection of the vessel to be placed at the needle. Selection: sample, sample+1, spec.#, 1...n Lift Position Start raising or lowering the lift to the specified position. Stop raising or lowering the lift to the specified position. Selection of the position. Selection: shift, work, rinse, special, 0, 1...
6 Devices Status display The current status of the 766 IC Sample Processor can be displayed on the Current state subtab of the Manual tab which is only available for a connected system. Sample Vial position Lift position Pump state Display of current sample position. Display of current beaker position. Display of current lift position in mm. Display of current pump state (on, off). Status of the 8 input lines Status of the remote input lines.
6.23 766 IC Sample Processor Num Task Parameter Row number (read-only). Program instruction (see below). Parameter for program instruction (see below). Enabled Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. List of program instructions The following program instructions can be added to the time program on the Program tab: IC Net 2.3 Instruction Parameter entry Meaning Move sample, sample+1, spec.#, 1...
6 Devices Ctrl "control command" Set the specified remote output signal. In addition to the normal control commands (details see 766 Instruction for Use) special commands defined on the Configuration tab can be used. Wait 1...9999 s Waiting time. StartDA Start data acquisition. Configuration The Configuration tab of the 766 IC Sample Processor window contains parameters settings on the five subtabs Rack, Vial positions, Needle positions, Control, and Scan.
6.23 766 IC Sample Processor Vial positions Rack type Current rack type (read-only). Parameter Name for special vial position. Number of special vial position. Pattern Add a new special vial position. Delete the selected special vial position. Needle positions Rack type Current rack type (read-only). Parameter Name for special needle position. Lift position of special needle position in mm. Pattern IC Net 2.3 Add a new special needle position.
6 Devices Control Pattern Name for special control command. Remote line settings for special control command. Add a new special control command. Delete the selected special control command. Parameter 184 IC Net 2.
6.23 766 IC Sample Processor Scan Pattern Name for special scan command. Remote line settings for special scan command. Add a new special scan command. Delete the selected special scan command. Parameter Links The Links tab of the 766 IC Sample Processor is used for COM port selection and settings (details see section 5.2.4 Links). IC Net 2.
6 Devices 6.24 6.24.1 788 IC Filtration Sample Processor 788 IC Filtration Sample Processor features The Metrohm 788 IC Filtration Sample Processor is a sampler for automatic filtration and injection of samples. It has a capacity of up to 127 samples each with an effective volume of up to 11 mL. The 788 IC Filtration Sample Processor, which is normally operated using the keypad of the instrument, can be fully operated using the «IC Net» program.
6.24 788 IC Filtration Sample Processor If the system is connected and the 788 icon is clicked with the right mouse button, the following menu appears: 6.24.3 Open Open the 788 IC Filtration Sample Processor window for parameter settings (this window can also be opened by double-clicking the icon). Timeout Open a window for setting the timeout time (in s) for the scan command. Unlink Delete the 788 icon from the system.
6 Devices Position Stop moving the rack to place the specified vessel at the needle. Selection of the vessel to be placed at the needle. Selection: sample, sample+1, spec.#, 1...n Lift Position Start raising or lowering the lift to the specified position. Stop raising or lowering the lift to the specified position. Selection of the position. Selection: shift, work, rinse, special, 0, 1...
6.24 788 IC Filtration Sample Processor Status display The current status of the 788 IC Filtration Sample Processor can be displayed on the Current state subtab of the Manual tab which is only available for a connected system. Sample Vial position Lift position Pump state Display of current sample position. Display of current beaker position. Display of current lift position in mm. Display of current pump state (on, off). Status of the 8 input lines Status of the remote input lines.
6 Devices Num Task Parameter Row number (read-only). Program instruction (see below). Parameter for program instruction (see below). Enabled Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. List of program instructions The following program instructions can be added to the time program on the Program tab: 190 Instruction Parameter entry Meaning Move sample, sample+1, spec.#, 1...
6.24 788 IC Filtration Sample Processor Ctrl "control command" Set the specified remote output signal. In addition to the normal control commands (details see 788 Instruction for Use) special commands defined on the Configuration tab can be used. Wait 1...9999 s Waiting time. StartDA Start data acquisition. Configuration The Configuration tab of the 788 IC Filtration Sample Processor window contains parameters settings on the five subtabs Rack, Vial positions, Needle positions, Control, and Scan.
6 Devices Vial positions Rack type Current rack type (read-only). Parameter Name for special vial position. Number of special vial position. Pattern Add a new special vial position. Delete the selected special vial position. Needle positions Rack type Current rack type (read-only). Parameter Name for special needle position. Lift position of special needle position in mm. Pattern 192 Add a new special needle position. Delete the selected special needle position.
6.24 788 IC Filtration Sample Processor Control Pattern Name for special control command. Remote line settings for special control command. Add a new special control command. Delete the selected special control command. Parameter IC Net 2.
6 Devices Scan Pattern Name for special scan command. Remote line settings for special scan command. Add a new special scan command. Delete the selected special scan command. Parameter Links The Links tab of the 788 IC Filtration Sample Processor is used for COM port selection and settings (details see section 5.2.4 Links). 194 IC Net 2.
6.25 Metrohm Spark Triathlon Autosampler 6.25 6.25.1 Metrohm Spark Triathlon Autosampler Triathlon Autosampler features The Metrohm Spark Triathlon Autosampler is a metal-free sampler with a built-in injection valve for automatic sample injection. It can handle sample volumes down to 5 µL, provides multiple injections from small sample volumes, ‘partial loop’ injections and automatic dilution.
6 Devices 6.25.3 Triathlon Autosampler window Triathlon icon / Open The Triathlon Autosampler window for parameter settings and manual control is opened by selecting this menu option with the right mouse button or by double-clicking the Triathlon icon in the SYSTEM window. It consists of the four tabs Manual, System settings, Methods programming and Links. Manual This tab contains the manual control for the Triathlon Autosampler which is organized in the three subtabs Main, Advanced and Actual states.
6.25 Metrohm Spark Triathlon Autosampler Rotate Sample Tray Rotate sample tray to access the tray segments. The segments can be arranged in any order on the tray, they will be recognized automatically if type and number of the segments are specified. Actual analysis time Rotate sample tray. Stop rotation of sample tray. Display of the time of a running analysis, starts with inject. Injector valve Switch the injector valve to the ‘load’ position.
6 Devices Advanced The manual control of the advanced features can be done on the Advanced subtab of the Manual tab. Code out AUX 4,3,2,1 Combo box to set the HEX output at the connector P3 TIMED OUTPUTS of the Triathlon Autosampler. Entry range: 0…15. Set the value specified above. Set the contact closures at the connector P5 AUXILLIARIES of the Triathlon Autosampler for remote control of four external devices. Value: 0 normally open 1 normally closed. Set the value specified above.
6.25 Metrohm Spark Triathlon Autosampler Tray cooling The tray cooling is normally not included with the Metrohm Spark Triathlon Autosampler, but it is available as an option. Current Tray temperature, °C Tray cooling Display of the actual tray temperature. Display of the cooling state. Only available if the tray cooling is installed. Method values Tray temperature, °C Tray cooling Combo box to select the tray temperature. Entry range: 4…40. Switch the tray cooling On or Off.
6 Devices For detailed information about the Triathlon Autosampler see Spark Triathlon user manual. 200 IC Net 2.
6.25 Metrohm Spark Triathlon Autosampler System settings This tab contains the configuration of the Triathlon Autosampler which is organized in the two subtabs General and Tray configuration. General The configuration of general system settings can be done on the General subtab of the System settings tab. Current Displays the actual value of each item. Method values Combo box or check box to select the value for each item. Loop volume, µL Volume of the sample loop. Entry range: 10…1000.
6 Devices Syringe volume, µL Volume of the syringe. Entry range: 100, 250, 500, 1000, 10000. Use the 10000 µL syringe only if the User Program is enabled on the Methods Programming/Template tab. The 10000 µL syringe is not supported by the predefined Triathlon methods. Syringe speed Aspirating speed of the syringe in injection methods, can be adapted to the viscosity of the sample. The syringe speed during wash or rinsing procedures are not affected by this setting. Entry range: LOW, NORMAL, HIGH.
6.25 Metrohm Spark Triathlon Autosampler Set the specified values. For detailed information about the Triathlon Autosampler see Spark Triathlon user manual. Tray configuration The tray configuration of the Triathlon Autosampler can be done on the Tray configuration subtab of the System settings tab. The sample tray is divided into eight segments. Each segment can be independently configured.
6 Devices
6.25 Metrohm Spark Triathlon Autosampler Methods programming This tab contains the configuration of pre-defined Triathlon methods and the programming of a free definable User program, it is organized in the four subtabs Template, Triathlon methods, User program, and Vials for Userprog and Mix method. Template On the Methods programming/Template subtab of the Metrohm Spark Triathlon Autosampler window method templates can be selected that are executed by the Autosampler during a determination.
6 Devices User program Enable the execution of the free programmable User program, the Triathlon methods are disabled. User specific program, all functions of the Triathlon Autosampler are available and can be combined to a program sequence. Notify system about injection Check box has to be activated to synchronize the injection on the Metrohm Spark Triathlon Autosampler with the recording of the chromatogram in the IC Net software.
6.25 Metrohm Spark Triathlon Autosampler Triathlon methods The Methods programming/Triathlon methods tab of the Metrohm Spark Triathlon Autosampler window contains the configuration of the four pre-defined Triathlon methods. Injection method The Methods programming/Triathlon methods/Injection/Injection subtab of the Triathlon Autosampler window contains the general settings of the pre-defined Injection method. Current Displays the actual settings of the Injection method.
6 Devices Entry range: 0…999 µL, default: 30 (with air segment) The minimum flush volume depends on the volume of needle and needle tubing, flush volumes of less than twice the volume of needle and tubing will result in decreased performance. Inj. volume, µL Programmable only for Partial and Pick-up modes, is equal to the Loop volume in Flushed mode. Is loaded from the sample queue.
6.25 Metrohm Spark Triathlon Autosampler The Methods programming/Triathlon methods/Injection/Pick-up meode’s options subtab of the Triathlon Autosampler window contains the definition of the transport vials required for the Pick-up mode. Transport Vials Basic settings for the transport vials. Transport Vial type Combo box to specify the type of segment that contains the transport vials.
6 Devices Last Transport Vial Combo box to specify the last transport vial position. Only active if Transport Vial = specified sample Vial + x is disabled. Position number range for the transport vials, the vial positions are used successively as a sample queue is processed. Transport Vial = specified sample Vial + Combo box to specify the position of the transport vial relative to the actual sample vial position. If activated, First Transport Vial and Last Transport Vial are disabled.
6.25 Metrohm Spark Triathlon Autosampler Method values Combo box to select the parameters of the Wash method. Method Parameter Wash volume, µL Entry range: 300…9999 µL Wash between Setting when the washing step takes place. Vials wash after a sample vial is processed Injections wash after every injection Apply parameter values selected under Method values to the Wash method.
6 Devices tor P5 AUXILLIARIES. The time program for each control line is arranged on a separate sheet. AUX-1,…AUX-4 Sheet for the time program of the corresponding control line. For each control line four events to set the contact closure to normally open (Aux-X OFF) and four events to set the contact closure to normally closed (Aux-X ON) can be programmed. Current Displays the actual settings of the Timebase program. Method values Check box to enable/disable the corresponding event.
6.25 Metrohm Spark Triathlon Autosampler IO-output codes Subtab for the settings to control the hexadecimal output at conncetor P3 TIMED OUTPUTS. Up to eight events 1…8 Code out can be defined. Current Displays the actual settings of the corresponding Code out event. Method values Field to select the settings for the corresponding Code out event. grey background field is inactive yellow background field is active Open the Code-out window to enter the values of the marked Code-out event.
6 Devices End time Subtab for the end time settings of the timebase program. Current Displays the current End time of the Triathlon autosampler. Method value Manual setting of the end time. The end time is sent to the Triathlon Autosampler with .
6.25 Metrohm Spark Triathlon Autosampler Apply values selected under Method values to the End time, the set values are displayed under Current. For detailed information about the I/O connections of the Metrom SparkTriathlon Autosampler see chapter 6 of the Spark Triathlon user manual. Mix method / User Program The Methods programming/Triathlon methods/Mix and the Methods programming/User program tab are structured the same way. They both allow configuring of liquid handling methods.
6 Devices Current Displays the current action sequence of the Triathlon Autosampler. The method’s action lot Programmable action sequence for the Mix method /User program. This sequence is send to the Triathlon Autosampler with . Syringe speed Speed of the syringe, 1 – 9. The resulting flow depends on the syringe size used. Load actual settings to the Triathlon Autosampler. Add an action to the method. Opens the Select action window. Edit a selected action of the method.
6.
6 Devices Vials for Userprog and Mix Method The Methods programming/Triathlon methods/Vials for Userprog and Mix method tab of the Triathlon Autosampler window contains the configuration of special vials for liquid handling methods. First destination vial 218 Specifies the transport vial for the liquid handling method. Use Enable/disable the usage of the destination vial in a User Program/Mix method. Segment type Define the type of tray segment that contains the first transport vial.
6.25 Metrohm Spark Triathlon Autosampler Position number of the transport vial, is successively increased by one as a sample queue is processed. Transport Vial = specified sample Vial + Combo box to specify the position of the transport vial relative to the actual sample vial position. If activated, First Transport Vial is disabled. Entry range: max. -95…+95, depends on type of tray segment. Reagent A…D Use Specifies the reagent vials, up to four reagents can be defined.
6 Devices 6.26 6.26.1 838 Advanced Sample Processor 838 Advanced Sample Processor features The Metrohm 838 Advanced Sample Processor is an autosampler for automatic sample injection in ion chromatography. Up to three dosinos can be connected to it via MSB-Connection. Besides the standard functions of an IC Sample Processor, it can handle automatic dilution/enrichment, inline calibration, partial loop injections and injection of very small sample volumina.
6.26 838 Advanced Sample Processor Unlink 6.26.3 Delete the 838 icon from the system. 838 Advanced Sample Processor window 838 icon / Open The 838 Advanced Sample Processor window for parameter settings and manual control is opened by selecting this menu option with the right mouse button or by double-clicking the 838 icon in the SYSTEM window. It consists of the four tabs Manual, Program, Configuration and Links.
6 Devices Selection: sample, sample+1, ext.#, next, prev, +rotate, -rotate, +swing, -swing, 1...151 move to the position set for parameter Sample at the bottom of this tab. sample+1: move to the position sample + 1. ext.1..4: move to the position ext.1..4 defined on the subtab Vial positions of the Configuration tab. sample: next: move the rack to the next position (+1). move the rack to the previous position (-1).
6.26 838 Advanced Sample Processor Number Selection of the rack position. Selection: 1...n For detailed information about the 838 Advanced Sample Processor see 838 Instructions for Use. Periphery Dosino Selection: Dosino MSB1, Dosino MSB2, Dosino MSB3 Choose dosino (connected to MSB1, MSB2 or MSB3). Selection: Dos, fill, release, prepar., empty, endVol, compen., port Dos: Dose volume. Selection: ± 0.001 ... 999.999 mL Selection of dosing volume. IC Net 2.3 fill: Fills the dosino cylinder.
6 Devices empty: Tubing system and dosino cylinder are emptied. endVol: Dosino cylinder contents are ejected. compen.: Cancels the mechanical play between the dosing piston and spindle. port: Choose port. Selection: 1, 2, 3, 4 Selection of the dosino port. Start function. Stop function. The dosing rate can be set in the DOSINO window, see 'Periphery Configuration' in this chapter.
6.26 838 Advanced Sample Processor Status display The current status of the 838 Advanced Sample Processor can be displayed on the Current state subtab of the Manual tab which is only available for a connected system. Autosampler Vial position Lift position Pump rate Valve position Input lines Output lines Display of current vial position. Display of current lift position in mm. Display of current pump rate. Display of current valve position. Display of current state of the 8 input lines.
6 Devices For detailed information about the 838 Advanced Sample Processor see 838 Instructions for Use. Time program On the Program tab of the 838 Advanced Sample Processor window a user-defined time program can be entered. This program is started automatically as defined in the Start mode window (see section 4.4.3) either at the moment the determination is started (Start with determination) or at the moment the sample is injected (Start with inject).
6.26 838 Advanced Sample Processor List of program instructions The following program instructions can be added to the time program on the Program tab: Instruction Parameter entry Meaning Move sample, sample+1, ext.#, +rotate, rotate, +swing, swing, 1...n Move rack to place the specified vessel at the needle (see 'Autosampler' in this chapter for an explanation of the positions). Lift shift, 0, 1...125 mm Raise or lower the lift to the specified position. Pump on (infinite), off, 1...
6 Devices lution (D) in the sample queue: Concentr. vol. = MV/D Dosino MSB1..3 rate 228 Dilution vol. Dose Dilution vol. (DV) = MV * (D-1)/D Reagent vol. Dose Reagent volume (R) defined on the tab Configuration/General. -Reagent vol. Aspirate Reagent volume (R) defined on the tab Configuration/General. fill Fills the dosino cylinder. release Prepares the dosino for changing the dosing unit. The dosing cylinder is filled via the rinse port.
6.26 838 Advanced Sample Processor Configuration The Configuration tab of the 838 Advanced Sample Processor window contains parameters settings on the six subtabs General, Vial positions, Needle positions, Control, Scan and Periphery. General Rack Name Code Vials IC Net 2.3 Choose the ordering number of the rack you are using. Binary ID code for rack identification (readonly). Number of maximum vials for the chosen rack type (read-only). Add a new rack to the list.
6 Devices Click button to open the ADD NEW RACK DATA window and add a new rack. Settings Rotate angle Swing angle Mixing volume Reagent volume Definition of the rotate angle needed for the command Move +/- rotate. Entry range: 0..270 °, default 5 ° Definition of the swing angle needed for the command Move +/- swing. Entry range: 0..180 °, default 5 ° Definition of the total solution volume after dilution. Entry range: 0.001…1000 mL, default 10 mL Definition of the volume of the reagent added.
6.26 838 Advanced Sample Processor Vial positions Special positions Vial number External positions Extern 1…4 Definition of special positions. Number of the special vial position. Add a new special vial position. Delete the selected special vial position. Definition of external positions. Definition of the rotation angle of the swing head for the external positions (1…4).
6 Devices Needle positions Needle positions Height, mm Lift position of special needle position in mm. Add a new special needle position. Delete the selected special needle position. Settings Lift rate Maximum lift way Lifting rate for the needle in mm/s. Lowest lift position possible in mm (readonly). Entry range: 0..235 mm, default 125 mm Maximum swing angle 232 Maximum swing angle possible in ° (readonly). Entry range: 0..
6.26 838 Advanced Sample Processor Control Pattern Name for special control command. Remote line settings for special control command. Add a new special control command. Delete the selected special control command. Parameter IC Net 2.
6 Devices Scan Pattern Name for special scan command. Remote line settings for special scan command. Add a new special scan command. Delete the selected special scan command. Parameter 234 IC Net 2.
6.26 838 Advanced Sample Processor Periphery configuration To edit the configuration of dosinos, stirrers and membrane pumps, click on the corresponding button to open the configuration window. Dosino IC Net 2.
6 Devices Define the alias name which appears in the rest of the driver. Default: Dosino MSB1…3 Dos port Select Dosino port for dosing. Entry range: 1, …, 4 , default 1 Fill port Select Dosino port for filling. Entry range: 1, …, 4 , default 2 Port 1...4 Configure dosino port Rate, mL/min Dosing rate of the corresponding dosino port. Entry range: 0.01 … 160 mL/min (can be max. the 3.
6.26 838 Advanced Sample Processor Alias Rate Define the alias name which appears in the rest of the driver. Default: Stirrer MSB1…3, Tower Stirrer Define stirring rate. Entry range: 1, … , 15 default 3 Send parameters to the devices. Membrane pumps EP1 Alias Configure membrane pumps. Define the alias name which appears in the rest of the driver.
6 Devices Links The Links tab of the 838 Advanced Sample Processor is used for COM port selection and settings (details see section 5.2.4 Links). 6.27 6.27.1 861 Advanced Compact IC 861 Advanced Compact IC features The Metrohm 861 Advanced Compact IC is a PC-controlled system for ion chromatographic analyses. The three following versions are available: • 2.861.0010 Compact IC without suppressor • 2.861.0020 Compact IC with suppressor module «MSM II» • 2.861.
6.27 861 Advanced Compact IC The 861 icon is available in the system window if a 861 Advanced Compact IC has been installed with the New system wizard or by using the Setup/New devices/Install new device option of the SYSTEM window (see section 6.1.3). If the system is connected, the 861 icon contains two buttons for manual control of the injection valve at the 861 Advanced Compact IC: Switch the injection valve to the "Inject" position. Switch the injection valve to the "Fill" position.
6 Devices Conductivity, µS/cm Live display of measured conductivity. Pressure, MPa Live display of measured pressure. The color settings for this two fields can be changed by clicking the fields with the right mouse button (see section 6.2.2). Display of actual values. Flow, mL/min Display of current flow rate of the highpressure pump. Pressure max, MPa Display of current maximum pressure limit for high-pressure pump.
6.27 861 Advanced Compact IC Peristaltic CO2 Suppressor Peristaltic pump. (inactive with version 2.861.0010) Start peristaltic pump. Stop peristaltic pump. 853 CO2 Suppressor (only displayed with version 2.861.0040) Start 853 CO2 Suppressor. Stop 853 CO2 Suppressor. System startup values The system startup values are sent and ap- plied to the 861 Advanced Compact IC each time the system is connected, a determination is started, or the values are sent manually with .
6 Devices The Program tab contains the two following subpages: Program Main time program with all program steps. Remote configuration Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. First column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry.
6.27 861 Advanced Compact IC Instruction Parameter entry Meaning Valve Inject, Fill Switch injection valve to "INJECT" or "FILL" position. FullScale 50, 250, 1000, 5000 Set full scale range to the seµS/cm lected value. ICPump on, off Switch on or off the high-pressure pump. Flow 0.2 ... 2.5 mL/min Set flow rate of the high-pressure pump to the desired value. Pmax 0.0 ... 35.0 MPa Set maximum pressure limit for the high-pressure pump to the desired value. Pmin 0.0 ... 35.
6 Devices Name (1st column) User-definable name of the remote command (e.g. Start_838). Remote command (2nd column) Setting the remote output lines 1…8. 0 (line off, inactive, open) 1 (line on, active, 0 V) p (pulse) * (leave line in current status) For entry of the first value, enter 1, 0, p or *. For entry of the other values, move the cursor in front of the value to be changed and enter 1, 0, p or *. Add new remote command. Delete selected remote command.
6.27 861 Advanced Compact IC 861 unit version Selection of instrument version: Suppressor Suppressor module: Automatic switching to the next position each time the injection valve is switched either to the Fill or to the Inject position. Autostep with Use peristaltic pump If this option is disabled, the peristaltic pump is not switched on automatically at the start of a determination or with Startup hardware. Use CO2 Suppressor (only displayed with version 2.861.
6 Devices The Hardware tab of the hardware settings window defines general parameters which are set automatically at power on of the 861 Advanced Compact IC. Remote lines after power on The remote output lines 1…8 are set to this values after power on or a manual stop with Shutdown hardware. Selection: 0, 1 Operating temperature of the conductivity cell.
6.27 861 Advanced Compact IC For a precise determination of the cell constant, pump a calibration solution of known conductivity through the IC system, observe the displayed conductivity and change the cell constant until the correct conductivity value is displayed. Possibility for changing the break times for injection valve Valve and suppressor module Suppressor.
6 Devices 1 (line on, active, 0 V) p (pulse output) * (leave line in current status) PowerOn values Display of the power on startup values for remote output lines set on the Hardware tab. Pulse length Length of a pulse in ms. Alarm stops The Alarm stops tab of the hardware settings window defines the events for which the 861 Advanced Compact IC is stopped immediately.
6.28 844 UV/VIS Compact IC 6.28 6.28.1 844 UV/VIS Compact IC 844 UV/VIS Compact IC features The Metrohm 844 UV/VIS Compact IC is a PC-controlled system for ion chromatographic analyses with UV/VIS detection. The four following versions are available: • 2.844.0010 • 2.844.0020 • 2.844.0110 • 2.844.
6 Devices Controls On the subtab Controls injection valve, peristaltic pump, highpressure pump, column heating and remote lines can be manually controlled. In addition, the parameters for the high-pressure pump and column heating temperature can also be set: Valve Peristaltic pump Injection valve Switch injection valve to "Inject" position. Switch injection valve to "Fill" position. Peristaltic pump Start peristaltic pump (only enabled, with versions 2.844.0020 or 2.844.120).
6.28 844 UV/VIS Compact IC 25.0 MPa (250 bar). Pressure min, MPa Value for minimum switch-off pressure for high-pressure pump. Once it has been set this value will be monitored, even if there is no connection to the PC (units depend on the settings made under IC NET / Global preferences). Entry range: 0.0 ... 35.0 MPa Column oven Column oven Turn column oven on (only enabled, if a column oven is connected). Turn column oven off (only enabled, if a column oven is connected).
6 Devices Turn VIS lamp off. Records a new zero spectrum. The zero spectrum is a reference spectrum. It should be recorded at a time at which there is no sample in the measuring cell. It is used for calculating the absorption spectrum. Opens the SPECTRUM window (see Section 6.28.5). Wavelength, nm Sets the wavelengths at which the chromatograms are to be recorded (for max. three measuring channels). Sets the bunching of the wavelengths defined above.
6.28 844 UV/VIS Compact IC Current state Channel 1 abs, mAU Live display of the absorption in channel 1. Channel 2 abs , mAU Live display of the absorption in channel 2. Channel 3 abs, mAU Live display of the absorption in channel 3. Ch1/Ch2 Live display of the absorption ratio of channel 1 and channel 2. VIS Lamp Live display of the status of the VIS lamp. UV Lamp Live display of the status of the UV lamp.
6 Devices determination) or at the moment the sample is injected (Start with inject). The Program tab contains the two following subpages: Program Main time program with all program steps. Remote configuration Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. 254 First column Time at which program instruction is applied. Entry range: 0.0 ... 999.
6.28 844 UV/VIS Compact IC Delete selected program instruction. Test the time program (error messages are displayed if program is wrong). List of program instructions The following program instructions can be added to the time program on the Program subpage: Remote 0, 1, *, p Set remote output lines 1...8 to the desired values. For entry of the first value, enter 1, 0, p or *. For entry of the other values, move the cursor in front of the value to be changed and enter 1, 0, p or *.
6 Devices Remote configuration On the Remote configuration subtab user-defined remote commands can be defined, which can be inserted into a time program after being activated with . Name (1st column) User-definable name of the remote command (e.g. Start_838). Remote command (2nd column) Setting the remote output lines 1…8. 0 (line off, inactive, open) 1 (line on, active, 0 V) p (pulse) * (leave line in current status) For entry of the first value, enter 1, 0, p or *.
6.28 844 UV/VIS Compact IC Startup with hardware The activated devices will be started with Startup hardware or at the start of a determi- nation. UV lamp If this option is switched off the UV lamp will not be switched on at Startup hardware or at the start of a determination. VIS lamp If this option is switched off the VIS lamp will not be switched on at Startup hardware or at the start of a determination.
6 Devices 6.28.4 Hardware settings Selection of the Hardware menu item using the right mouse button opens the hardware settings window consisting of the four tabs Outputs, Alarm stops, System settings and Detector settings. Outputs The Outputs tab of the hardware settings window defines remote output signals to be set automatically if specific events occur. Event Inject Fill Events for automatic remote signal output: Switching of the injection valve to the "INJECT" position.
6.28 844 UV/VIS Compact IC are stopped immediately, the running determination and the active sample queue are also stopped. Shutdown hardware after.. Pump stop Hardware error Leak Power on IC Net 2.3 Events for alarm stop: Pump stopped because pressure limits are exceeded. Hardware error detected at the 844 UV/VIS Compact IC (high-pressure pump, injection valve, or suppressor not working correctly). Leak detector has detected solvent in the instruments interior.
6 Devices System settings On the tab System settings break times for the injection valve and flow rate corrections for the high-pressure pump are defined. Valve Break time, ms Pump Flow correction Injection valve. Modification of the break time for the injection valve. Entry range: 1 ... 99 ms High pressure pump. Factor for correction of the difference between displayed and actual flow rate of the high-pressure pump. Range: 0.9 ... 1.
6.28 844 UV/VIS Compact IC Detector settings On the tab Detector settings measuring settings are defined. Detector settings VIS lamp T int, ms T out, s Data rate Settings for UV/VIS detector. Display of the voltage of the VIS lamp. Integration time. Entry range: 10 ... 254 ms Measuring time per data point. Entry range: 0.05 ... 2.00 s Effective measuring time per data point. The Data rate is the particular whole number multiple of the value T int that is closest to the value T out.
6 Devices Operating hours UV lamp, h VIS lamp, h 6.28.5 Operating hours of the lamps. Display of the operating hours of the UV lamp. Set operating hours of the UV lamp to 0. Click it after replacing the UV lamp. Display of the operating hours of the VIS lamp. Set operating hours of the VIS lamp to 0. Click it after replacing the VIS lamp.
6.28 844 UV/VIS Compact IC ample for the wavelength set for measuring channel 1: Abs = log Int λ1zero Int λ1akt where: Intλ1zero The intensity measured when recording the zero spectrum at the wavelength set for measuring channel 1. Intλ1akt The actual intensity measured at the wavelength set for measuring channel 1.
6 Devices Opens the dialog for entering the path and file name. Saves the last recorded spectrum in the selected .cvs-file. Format: 2 lines of floating numbers (first line: wavelength, second line: absorption or intensity). Copies plot into clipboard. Closes window. If a right-hand mouse click is made in the spectrum window the wavelength Current wavelength and the corresponding absorption value Current spec. value will be shown. 6.29 6.29.
6.29 Data recorder 6.29.2 Select processing method and data source The Data recorder icon / Setup menu item opens the following window: Processing method Name of the method file (*.mtw) linked to the system. Open processing method. Select the method file (*.mtw) to be linked to the system. Connected data source Data source for main measurement chan Connected nel. Open the Data source window for selection of the data source: Connected data source.
6 Devices # ch1 and ch2 761 Compact IC # Cond # Press 732 IC Detector # Cond # Abs.Cond PC Board# # ch1 and ch2 817 Bioscan # Cell Data source channels of the 771 Compact Interface. Conductivity channel of the 761 Compact IC. Pressure channel of the 761 Compact IC. Conductivity channel of the 732 IC Detector. Absolute conductivity channel of the 732 IC Detector. Data source channels of the PC Board 1 or PC Board 2. Cell potential channel of the 817 Bioscan. Temperature channel of the 817 Bioscan.
6.30 System timer 6.30 6.30.1 System timer Timer icon The system timer is used for programming system tasks which are started automatically daily or once at the desired time. The System timer icon is available in the SYSTEM window if a Timer has been installed with the New system wizard or by using the Setup/New devices/Install new device option of the SYSTEM window (see section 6.1.2). 6.30.2 Timer program The timer program of the system program is identical to the global timer program (see section 5.
7 Methods 7 Methods A method contains all information necessary for data acquisition, integration, peak evaluation and quantification. It can be considered as the chromatogram template, i.e. chromatogram without raw data. Methods are stored as method files (*.mtw) in the Methods directory. Each system is linked to a method. This method is called processing method and is opened automatically at the start of a new determination. 7.
7.2 Passport 7.2.1 General Tab General of the passport with general description of the method and determination. Ident Duration METHOD DATA Date/time Last update Calibration level User Detector Run IC Net 2.3 User defined identifier (title) for the chromatogram to be displayed in the title bar of the chromatogram window and in the Chromatogram open window. Duration of the chromatogram in min. You can modify this value when the chromatogram is running.
7 Methods To reset the Run number press [Ctrl] [F8]. The Analysis number window is opened where the run number for the next run can be modified to the desired value. Batch 7.2.2 X/Y: total number of started sample queues / current analysis number in the sample queue (read-only). Sample Tab Sample of the passport with sample information. Info 1 Info 2 Volume Dilution Vial number Amount 270 First sample description (max. 256 characters). Second sample description (max. 256 characters).
7.2 Passport Internal standard amount Concentration of the internal standard component for relative concentration calculations. Date/time when... 7.2.3 Date and time of sample collection (the default values are equal to the date and time when the chromatogram starts). Column Tab Column of passport with column information. Number ID Length Serial number of column (max. 256 characters). Internal diameter of the column in mm. Length of the column in mm. This parameter is used to calculate Linear flow.
7 Methods 7.2.4 Eluent Tab Eluent of passport with eluent information. Eluent A Eluent B Eluent C Flow Pressure Temp. 272 Description of eluent composition (max. 256 characters). Description of eluent composition (max. 256 characters). Description of eluent composition (max. 256 characters). Flow rate of the high-pressure pump in mL/min or µL/min. The flow rate is used to recalculate the time axis into volumetric units.
7.2 Passport 7.2.5 Comment Tab Comment of passport for entry of free-text user comments into the chromatogram description. Use this feature to enter any additional information about the chromatogram not included in other sections of the method. IC Net 2.
7 Methods 7.3 Method setup IC NET / Method / Method setup This menu item opens the Method setup window which includes the most common parameters for data acquisition and evaluation of the method and consists of the following tabs: General Measure Filters Processing Export Math 7.3.1 General information on determination. Important data acquisition parameters. Parameters for noise filtration. Set actions that are performed when the chromatogram finishes. Export options of the chromatogram.
7.3 Method setup Start delay Time delay before starting data acquisition in min. User-defined detector name. Data acquisition source Measuring channel (read-only). Detector name 7.3.3 Filters Tab Filters of Method setup window with parameters for noise filtration of raw data. Three different filtration algorithms are available to reduce noise and increase apparent signal-to-noise ratio. Spikes Slit Median Slit Gaussian IC Net 2.3 Spikes filter.
7 Methods Raw data points Smallest peak has ... 7.3.4 bit smaller and wider after smoothing, but their area does not change. Number of data points per chromatogram (read-only). Number of data points per smallest peak on the chromatogram (read-only). This information is valuable to perform the Compress procedure (data compression). Processing Tab Processing of Method setup window for definition of actions that are performed during the run or when the chromatogram finishes.
7.3 Method setup Optimize your analysis first to get stable measuring conditions without a retention time drift. In any case it is better to have stable conditions, use this feature only as a last possibility. Calibration All Show all Make report Close window If checked, the retention times will be updated in the peak table after every calibration. If checked, the retention times will be updated in the peak table after every determination.
7 Methods 7.3.5 Export The tab Export of Method setup window contains the export options for chromatograms that are performed during the run or when the chromatogram finishes. The export to the target application selected is performed when the chromatogram is saved, which is set at IC Net / Method / Method setup / Processing under Save chromatogram after the run or while running save every ... min.
7.3 Method setup to use this feature. Depending on the Excel version in use, settings of Excel have to be modified. For detailed information how to configure Excel, see the file ICNet_ExcelReport_ ExcelSettings.pdf in the folder ..\IC Net\ExcelReport\. 7.3.6 Math Tab Math of Method setup window with parameters that are used for various types of calculations. Parameter Formula set Theoretical plates Resolution Asymmetry Formula Custom formulae IC Net 2.
7 Methods European Pharmacopoeia Formula setting according to European Pharmacopoeia. US Pharmacopoeia Formula setting according to US Pharmacopoeia. Calculation of void time/void volume: Calculation method Selection of the method for void time calculation: None Manual entry in the Void time field. 1st component The peak corresponding to the first component is selected as a void time marker and its retention time replaces the previous value of the void time.
7.4 Integration 7.4 Integration 7.4.1 General information IC NET / Method / Integration This menu item opens the Integration parameters window which contains parameters and events for peak integration and consists of the two tabs Setup (see section 7.4.2) and Events (see section 7.4.3). The «IC Net» software includes a built-in automatic integration algorithm to detect peaks on the chromatographic curve and to evaluate them using calculated baselines.
7 Methods 7.4.2 Setup Tab Setup of Integration parameters window with integration parameters. The two most important of these parameters for peak recognition are Width and Slope. If the signal-to-noise ratio of the chromatogram is high enough (e.g. 100:1 or higher), the integration algorithm is not too sensitive to changes in integration parameters. On the contrary, if the baseline noise is high, careful tuning of these parameters may be needed. Number of peaks Number of peaks detected (read-only).
7.4 Integration Broadening Slope Asymmetry Min area Min height Rider ratio Negative peaks width of the narrowest peak on the chromatogram (normally 2…10 s). Entry range: 0.1…480 s Broadening for peaks in the end of the chromatogram compared to peaks in the beginning. This parameter is used for automatic adjustment of Width. Entry range: 0.1…100 Threshold for peak recognition.
7 Methods 7.4.3 This function sets up reasonable integration parameters in the following way: • Slope is set to a reasonable value. • Asymmetry is set to a reasonable value. • Width and Broadening are calculated by fitting a straight line through the measured peak halfwidth values vs. retention time. Width is determined as the y value of this line at the Delay time, Broadening is calculated as the y value at the end of the chromatogram divided by the y value at the Delay time.
7.4 Integration If an integration event is added or modified, the Edit integration event window is opened where the following parameters can be set: Time Event Value Start time for integration event in min. List box with integration events to be selected (see below). Parameter value for selected integration event. This field appears for events that demand input of an additional parameter. Integration events for peak detection Set this mode to stop detection of new peaks.
7 Methods Integration events for peak start/end Force the beginning of a new peak. If a peak is available at the moment of the event it is either rejected (upslope) or terminated (downslope). Set peak end Force peak end at the time of the event. Upslope peaks are rejected (except those born by Set peak start event), downslope peaks are terminated. Stop single peak mode Disable peak end detection. All peaks of any group after this event will be treated as one peak. The group end will be treated as peak end.
7.4 Integration Set horizontal baseline Set horizontal baseline for all peaks except the last one of adjacent peaks that are not separated. The baseline is drawn forwards from the peak start point. Set back horizontal base Set normal baseline Set horizontal baseline for all peaks except the first one of adjacent peaks that are not separated. The baseline is drawn backwards from the peak end point. Set default baseline detection mode. Set baseline point at 4.5, 6, 7.2, 8.9 and 9.
7 Methods Force horizontal base back Cancel horizontal base back Force horizontal base back Set horizontal baseline for a single peak. The baseline is drawn backwards from the peak end point. The intersection of the baseline with the signal is defined as peak start point. Cancel horizontal base back Clear Force horizontal base back mode. Enable baseline penetration Disable baseline penetration Enable baseline penetration Allow crossing of the signal by the baseline.
7.5 Calibration and quantification 7.5 Calibration and quantification 7.5.1 General information The aim of any chromatographic analysis is to answer the question "What components are present in the sample and what are their concentrations?". Two procedures are used to achieve this goal: the first step is called calibration, the second step includes quantification.
7 Methods 7.5.2 Notations R Stands for response value, either area or height, depending on setting selected in the Calibration graphs window. V Sample Volume injected. D Dilution coefficient, shows number of times to which the initial solution is dissolved before injection. V' = V / D Adjusted volume of injected sample. A correction is made for the dilution coefficient. C Concentration of the component in the initial solution (before dilution).
7.5 Calibration and quantification 7.5.3 External standard calibration External standard calibration (absolute calibration) is a key way of calibration. Basically this procedure calculates the dependence of injected component's quantity versus area (or height) of the corresponding peak. This dependence is showed as a calibration plot with injected quantity along the Y axis and peak area (or height) along the X axis.
7 Methods 292 Number Row number (read-only). Peak Number of the peak in the chromatogram that corresponds to the given component. If this field contains a 0, the peak number must be entered manually. Time Expected retention time of the component in the calibration run. After a new entry of the peak number the corresponding retention time is entered automatically as soon as this field is clicked on with the mouse. Wind.% Identification window of the component.
7.5 Calibration and quantification min C (max C) Minimum (Maximum) concentration value for the component. Components whose concentration leaves the range min C…max C are marked in the peak table by the sign " ! ". Additionally, the component table contains the following buttons: Add a new component (an empty column) to the component table. Delete the current component from the component table. Open the Peak identification window (see section 7.5.5).
7 Methods 7.5.5 Peak identification IC NET / Method / Calibration / Identification Selecting this menu item or clicking on in the component table opens the Peak identification window with parameters for tuning the peak identification procedure. Number of components Number of components in the component table (read-only). Scheme Standard Nonstandard Identification Reference peaks Other peaks Identification parameters. Identification parameter for reference components.
7.5 Calibration and quantification Worst case... Information on the component with the worst (largest) deviation of actual and expected retention time. Deviation is given as a part of component’s identification window. Average relative deviation Relative deviation averaged for all components. 7.5.
7 Methods Level 1...Level N Contains concentrations (or other chosen values) of components for corresponding Calibration level. Add a new Calibration level to the concentration table. The following window appears: Create calibration level Number of calibration level to add. Fill level with concentration Concentration to be filled in for all components. Copy concentrations from level Number of calibration level from which concentrations are copied.
7.5 Calibration and quantification 7.5.7 Calibration curve IC NET / Method / Calibration / Graphs Selecting this menu item or clicking on in the component table opens the Component window. In this window the results of the calibration are shown for each component together with the calibration curve. The parameters for calculating the calibration curve can also be entered here.
7 Methods Calibration points table This table contains the basic information used to construct the calibration curve: Level Number of Calibration level. Conc. Concentration of the current component in the calibration sample. It is taken from the concentration table. Area (or Height) Peak height or area of the current component, depending on calibration base. File File name of calibration chromatogram that stores data on the given calibration level.
7.5 Calibration and quantification Base Base for calculations (Area or Height) in quantification and calibration procedures. Indicates, which parameter is to be used as a peak response. Reference channel Channel which is used to measure area or height (read-only). Formula Formula for calibration function. There are six possible calibration dependencies for linear and nonlinear calibration curves.
7 Methods amount of the injected components becomes equal to the declared value. This declared summary amount is entered into the Concentration field of the universal component for This run level. 7.5.9 Calibration data handling Load and save calibration data IC NET / Method / Calibration / Load from method Load the calibration from the method. This option is used to update the calibration for the current run by the one taken from the method used for acquisition.
7.6 Report output 7.6 Report output 7.6.1 Report options window IC NET / Method / Report options IC NET / Process / Make report The Report options window is divided into several regions that combine parameters for report output on their functionality and includes several different areas. The report output is started by clicking on . IC Net 2.
7 Methods 7.6.2 Items to report This part of the Report options window contains a list of important report items that can be included into the report on the user's choice: General Report date: Printed by: General description on the analysis from the Passport. The Report date and the logged-in user Printed by are also put out. 07/12/1999 15:59:10 Roland Dörig Ident: Tap water Analysis from: 10/11/1999 16:17:02 File: jb101617.chw 16/11/1999 15:02:14 Modified! Method: Multicalibration.
7.6 Report output Chromatogram plot Plot of the chromatogram (only for Printer or File destination). Peak table Peak table with results. The structure of the peak table depends on the selected Quantification method. The following report is put out as default: Quantitation method: Custom No Retention Height Area Conc. min mV mV*sec mg/L 1 3.04 1.85 21.062 0.079 2 5.91 82.44 1703.350 7.173 3 6.83 3.58 97.495 0.000 4 14.29 29.27 969.705 7.561 5 21.21 19.34 935.179 5.
7 Methods 7.6.3 More items to report This part of the Report options window contains a list of rarely used report items that can be included into the report on the user's choice: Acquisition Parameters used for data acquisition from the from the General, Measure and Filters tabs of the Method setup window. ACQUISITION PARAMETERS Channels: Method duration: Run duration: Measurements (method): Measurements (run): Freq.
7.6 Report output Component table No Retention Window% 1 2 3 4 5 6 7 3.05 5.92 8.70 11.19 14.60 16.80 21.26 5.0 5.0 5.0 5.0 5.0 5.0 5.0 RF Conc. 2.623e-03 4.356e-03 6.691e-03 9.933e-03 7.912e-03 1.556e-02 6.021e-03 0.08 7.17 0.00 0.00 7.56 0.00 5.57 Calibration results CALIBRATION OF COMPONENT Method: Equation: RSD: Correlation Component table. Index Type Group 0.000 0.000 0.000 0.000 0.000 0.000 0.
7 Methods Name Units Input Minimum Zero Maximum Range Coefficient Noise Shift (multi)Channel ratio 306 Channel’s name. This text appears as Channel label in the chromatogram. User-defined units of detector response. Number of data acquisition channel. Minimum value of the linear range of the AD converter (in ADC conversion units). This parameter is used to detect an underflow condition. A signal level on a baseline (in ADC conversion units). This parameter is a result of the ADC calibration.
7.6 Report output 7.6.4 Report destination The following output devices can be selected simultaneously in any combination as targets for report output: Screen Printer File 7.6.5 Output report (without curves) to screen. Output report to printer. Output report to file (see section 7.6.7).
7 Methods As long as no universal component is defined, only peaks with non-zero concentrations are listed. The peak table includes the following columns: Peak number, Retention time, Height, Area, Response factor, Concentration, Concentration%, Name Relative concentration Index Column test Custom Standard component 308 This quantification method uses the internal standard method to calculate the relative concentrations of the components.
7.6 Report output Concentration of std Concentration of the internal standard component for relative concentration calculations. This value is stored in This run column of the concentration table. Total % for normalization A value to which the sum of concentrations is normalized. It is used for Response normalization and Normalized concentrations quantification methods. The default value is 100. Printing order By peaks By components IC Net 2.3 Determines the order of the components in the peak table.
7 Methods 7.6.6 Template options For report output using a report template the following parameters can be modified: 7.6.7 Template Selection of the report template file *.rtt. Several templates for different languages and applications are available. Separator Defines which character separates report columns in tables. A separator other than Space is useful when the report is printed to a file and this file is imported into a spreadsheet software. Tab size Sets tab stop value for the screen window.
7.6 Report output 7.6.8 Report elements If Quantification method = Custom has been selected, the peak table can be customized on the user's choice. After clicking the button, the following columns can be included in the peak table. number Peak number. retention time Retention time of the component (in minutes, irrespective of the chosen retention units on the chromatogram graph axes). The total value in the column is equal to the chromatogram duration.
7 Methods where i and i+1 indexes refer to the neighboring peaks, and w0.607 stands for the peak width at 60.7 % of the peak height. effectivity, TP Effectivity for the peak in number of theoretical plates. The number of theoretical plates Ni per column for a chosen peak is calculated for a chromatographic peak by one of two formulas: Ni = 2 PI (ti • Hi / Ai)2, where PI = 3.1415926..., ti = retention time, Hi = height, Ai = area of the peak. The more commonly used formula is: Ni = 5.
7.6 Report output asymmetry PGF = 1 for an ideal peak with gaussian shape. Peak asymmetry As is calculated at 1/10 of the peak height as a ratio of width after the top of the peak w2 to the width before the top w1. As = w2 / w1 response factor Coefficient k1 of the calibration curve. raw concentration Absolute concentrations (raw quantity) of the components, calculated as Ci = Wi(Ri) / V' Total value for the column is the sum of concentrations for all components.
7 Methods peak amount Absolute amount of substance which is contained in the sample loop volume. It is the product of the absolute concentration C(i) multiplied to the volume of the sample loop. The amount is displayed without unit in the report, pay attention to the order of magnitude of the displayed value! index Linear or logarithmic retention index for identified components. Total value for the column is a weighted index average with absolute concentration as weight. I = ∑ { (Ii Ci) / ∑ Ci }.
7.6 Report output The complete component type may look like: BBD : !R. IC Net 2.3 group Number of the group for the component. spectral ratio Ratio of responses on different channels for multi-channel chromatograms. name Name of the component. file name File name of chromatogram. This column is very useful for the purpose of processing of exported data. ident Title (identifier) of the chromatogram. This column is useful for the processing of exported data.
8 Chromatograms 8 Chromatograms 8.1 Chromatogram window The CHROMATOGRAM window is used to show a running or recorded chromatogram. Title bar Status bar Chromatogram The title bar of the chromatogram window contains buttons for minimizing, maximizing and closing the window. The window name consists of the elements "Ident (method name) chromatogram name". A star ( * ) at the end of the name indicates that the chromatogram has been changed since the last saving. The status bar contains two fields.
8.2 Chromatogram file handling During active data acquisition the elapsed time, the duration of the chromatogram or program and the current X and Y values are shown in the second field of the status bar. If the peak editor is switched on then the position of the cursor will be shown. A chromatogram can be scaled with the help of keyboard or mouse functions or through the Chromatogram axes window opened with IC NET / View / Appearance / Chromatogram axes.
8 Chromatograms Filename Selected Directory Filename (common wildcards as * and ? are possible). Number of selected chromatograms and their total size. Path name of the working directory File window Box with the list of files residing in the working directory sorted by time (the last recorded chromatogram is at the top of the list). As additional short information on the chromatogram the two parameters Ident and Method are also displayed.
8.2 Chromatogram file handling 8.2.2 Save chromatogram IC NET / File / Save / Chromatogram Save the selected chromatogram in a chromatogram file (*.chw) in the working directory. If this chromatogram has been already stored in this directory, the message File ... exists. Overwrite? appears. If it is preferable to overwrite the previous copy (for example, after the reprocessing), press the button. Pressing saves the chromatogram into the new file (a new copy of the chromatogram is created). 8.2.
8 Chromatograms Represent raw data as Output of raw data: Default units (float) Y values are exported in default units for the channel. ADC response (integer) Y values are exported in bits. Include retention time In seconds (float) As point number 8.2.6 X values are exported in seconds. X values are exported as point number. Import chromatogram IC NET / File / Import / Chromatogram Import chromatogram of the following formats: *.chm Chromatogram files of the «Chrom&Spec» program (DOS version). *.
8.3 Graphical representation 8.3 Graphical representation 8.3.1 Appearance IC NET / View / Appearance This menu item opens the Appearance window. It determines the appearance of the chromatogram and consists of four tabs: Chromatogram axes Labels Select channel Colors Scaling of chromatogram axes. Settings for peak labels and baseline drawing. Select channels to be displayed (only available for multi-channel chromatograms). Color settings for chromatogram elements.
8 Chromatograms Response axis Y from Y to Tic marks Beginning of the window on Y axis. End of the window on Y axis. Plot axes labels and tic marks on Y axis with no, relative or absolute scaling. View all Set scales on X and Y axes so that the whole chromatogram is visible. Drift compensation Turns the chromatogram screen image so that the last and first points of the chromatogram are on the same level. This option is useful for gradient chromatograms.
8.3 Graphical representation Component name Name of the component. Component name + Quantity Name and quantity of the component. Channel labels At start In corner Label for data acquisition channel. Channel label is shown at the start of the chromatogram. Channel label is shown in the upper right corner of the chromatogram. Show baseline and peaks Baselines are shown. Baseline marker Start and end of baselines are marked. Label always visible Label is shown always with zooming.
8 Chromatograms Colors Tab Colors of the Appearance window with color settings parameters for chromatograms. Hair Background Axes Baseline AlterGround Channel 1...8 Line width Line width for the selected element (Axes or Channel 1...8) in % of selected plot font height. Range: 0 ... 250 Set all Set colors of all opened chromatograms automatically to the settings in the selected chromatogram window. Set colors in the selected chromatogram window to the set defaults colors.
8.3 Graphical representation By selecting this menu item or pressing [ Ctrl ] + [ Home ] the X axis is scaled so that the chromatogram perfectly fits the window horizontally. IC NET / View / Y full scale By selecting this menu item or pressing [ Ctrl ] + [ End ] the Y axis is scaled so that the chromatogram perfectly fits the window vertically.
8 Chromatograms 8.4 Peak editor 8.4.1 Switching on/off the peak editor IC NET / Process / Peak editor The peak editor mode is used for subsequent manual correction of the automatic integration for chromatogram peaks (see section 7.4). It can be used to select the most important points for the peak evaluation (start, end and top of peak, valley between peaks) and move them to the required position. If the peak editor mode is enabled, the vertical bar cursor appears in the chromatogram window.
8.4 Peak editor IC NET / Peak / Select end point Move the cursor to the end of the nearest peak and select the peak. IC NET / Peak / Select valley point Move the cursor to the valley of the two nearest peaks. IC NET / Peak / Unselect peak Delete the selection. [–] Move a selected point (start, top, end, valley) to the cursor position. IC NET / Peak / Move selected point [+] Merge two neighboring peaks into a single peak.
8 Chromatograms 8.5 Printing 8.5.1 Page layout for printing IC NET / File / Page layout The selection of this menu item or clicking on in the Report options window opens the Page layout window for page layout parameters. Length units inches centimeters Page margins Left Right Top Bottom Selection of length units. Inches. Centimeters. Selection of page margins. Left margin. Right margin. Top margin. Bottom margin.
8.5 Printing 8.5.2 Save set page layout parameters as default values. Printer settings IC NET / File / Printer setup By selecting this menu item the Print setup window is opened where printer, paper size and format can be defined. 8.5.3 Print preview IC NET / File / Preview By selecting this menu item the Preview window appears on the screen where the report is shown in the appearance formatted for the desired printer as defined in the Report options window. 8.5.
8 Chromatograms 8.6 Miscellaneous functions 8.6.1 Reintegration IC NET / Process / Reintegrate This menu item opens the Integration parameters window where the reintegration of the chromatogram can be started (details see section 7.4). 8.6.2 Recalibration IC NET / Process / Calibrate This menu item opens the Recalibration window for entry of the calibration level and recalibration of the chromatogram using this level (details see section 7.5). 8.6.
8.6 Miscellaneous functions Meaning Meaning of the signature, can be selected from a pop-up list that is predefined in the Meaning set window. Actions Sign the chromatogram. Revoke the signature. Once a chromatogram is signed it cannot be altered. To alter a signed chromatogram the signature has to be revoked. Close the Electronic signature window . Opens the Meaning set window.
8 Chromatograms IC NET / Process / Electronic signature This window displays the signatures of the chromatogram in the active window. The signature table can be sorted according to one of the five columns by clicking the title. A star ( * ) at the end of the title indicates the sorting criterion. 8.6.
8.6 Miscellaneous functions 8.6.5 Compare chromatogram IC NET / Process / More / Compare This menu item opens the Compare with window that allows the comparison of the active open chromatogram to any other opened chromatogram or method. The chromatogram (or method) that is to be compared has to be the active window. Compare the selected file of the list to the chromatogram (or method) of the active window and display the result in the Differences window.
8 Chromatograms File Close Close window and return to Compare with window. Edit Copy Left frame Right frame 8.6.6 Copy the data of the comparison to the clipboard. Overview of the found differences, arranged in a menu tree. Click on the interesting menu point to display its properties in the Right frame. The displayed menu point is inverted. Table of the property values of the selected menu point.
8.6 Miscellaneous functions 8.6.7 Invert chromatogram IC NET / Process / More / Invert! This menu item inverts the response curves for all channels of the chromatogram so that negative peaks become positive and vice versa (useful for chromatograms with wrong input polarity). 8.6.8 Autodatabase IC NET / Method / Autodatabase The Autodatabase options window contains parameters and settings for sending chromatograms to the Autodatabase program. Database file Definition of the database file (*.
8 Chromatograms Close the window and abandon changes. For details on the «Autodatabase 1.0» program, open this program and call on-line help. IC NET / Options / Indicate AutoDB server Indicate Autodatabase program icon on the task bar if it has been already activated in the background by sending data. 8.6.9 Excel report IC NET / Method / Method setup / Export / Export data to Excel … / Report options / Template: Excel.
8.6 Miscellaneous functions Excel file The Excel file ExcelReport.xls is located in the folder ..\IC Net\ExcelReport\. Formatting, how the data is displayed is done in this file. The file is saved every time new data is written in. It consists of the following components: Charts Import worksheet Calculation worksheet Import worksheet data Worksheet where data from «IC Net» is filled in.
8 Chromatograms Make sure in «IC Net» that the item corresponding to the first column always contains information, otherwise a line with no entry in the first column may be overwritten. Charts Chart name 1-n (concentration fluoride,….concentration sulphate on the default files) The number of charts, the name of the charts and which data are displayed in the charts are specified in the [Header] section of ExcelReport.ini, the charts are built from the data of the data worksheet.
8.6 Miscellaneous functions IC Net 2.3 [Items] Specifies columns, their headers and the order of the columns in the data worksheet. [Ions] Specifies the ions for which data is reported in the Excel report. [Charts] Specifies the charts that are displayed using the data of the data worksheet. [Files] General settings for the Excel report.
8 Chromatograms [Item] TableStart = A6, Specifies the cell in the data worksheet of the ExcelReport.xls where the header of the first column is located. Defines the position of the data cells of the data worksheet. For correct display of the data in the ExcelReport.xls the formatting of the cells has to match the containing data type. Remember to adjust the formatting when changing the value of TableStart, important especially for correct display of DateTime data.
8.6 Miscellaneous functions Rel.conc. Relative (Internal Standard) concentration. Index Linear or logarithmic index. Type Marks some properties of the peak. Group Number of the group for the component. File File name of chromatogram. Ident Ident (title) of the chromatogram. DateTime Date and time when the chromatogram was recorded. Pressure Pump pressure at the beginning of the determination. Sample1 Sample Info 1 specified in the Passport. Sample2 Sample Info 1 specified in the Passport.
8 Chromatograms The names of the components for which data is to be exported to the Excel file have to be exactly the same (case sensitive!) in the component table of the «IC Net» method and here. [Charts] NoCharts = n, number of charts that created from the data worksheet. X1 = Y1 . = . . . . Xn = Yn = n, pair of axes of the chart 1 – n., the number after = specifies the header number of the 1st – nth column of the data worksheet and therefore defines which data is assigned to each axis.
9.1 Sample queue file handling 9 Sample queue A sample queue is a table containing sample-specific data which is used to facilitate work with autosamplers and multiple analyses. The number of rows of the sample queue table defines the number of determinations which are run automatically. Once the queue has been started, the sample-specific data are transferred line-by-line to the running determination overwriting the corresponding fields of the method. A sample queue is stored in a sample queue file *.
9 Sample queue 9.2 Sample queue control 9.2.1 Sample queue overview table IC NET / File / Open / Sample queue SYSTEM / System / Sample queue These two menu items open the OPEN window for opening an existing sample queue file *.que or creation of a new sample queue file *.que by entering a new name. After confirmation with the sample queue overview table is opened showing the current status of the sample queue: No System Ident Vial Inj-s Started 344 Row number.
9.2 Sample queue control Level Volume Amount Calibration level (see section 7.5) for the sample: Level 0 stays for normal analysis, levels 1 and greater for calibration runs. Correct filling of this column enables to calculate calibration coefficients automatically. Injected volume of sample in µL. Sample amount.
9 Sample queue Interrupt execution of the sample queue after the current determination has been finished. A running sample queue can be interrupted with . When the current determination has been finished, the sample queue can be restarted with or stopped with Control / Stop determination in the SYSTEM window. 9.2.4 Cancel last run SAMPLE QUEUE OVERVIEW / Control / Cancel last run Cancel the last run (set Started = 0 for this row). If the queue is restarted, this row is executed again.
9.3 Sample queue editor All system files of the sample queue must be stored in the same system subfolder. Ident Vial Volume Dilution Amount User defined identifier for chromatogram. Will be placed into appropriate passport field when starting the chromatogram. Autosampler vial position to take sample from. Injected volume of sample in µL. Sample dilution prior to injection. Sample amount.
9 Sample queue Cut the selected rows of the sample queue table and copy them into the clipboard. QUEUE EDITOR / Edit / Copy row(s) Copy the selected rows of the sample queue table into the clipboard. QUEUE EDITOR / Edit / Paste row(s) Paste the rows from the clipboard into the sample queue table. QUEUE EDITOR / Edit / Delete row(s) Delete the selected rows from the sample queue table. QUEUE EDITOR / Edit / Duplicate row(s) Duplicate the selected rows of the sample queue table.
10.1 Batch reprocessing queue file handling 10 Batch reprocessing Reprocessing is understood to be the subsequent reprocessing of a series of chromatograms which have been loaded in a queue (Batch reprocessing queue). For reprocessing according to a selected method the settings for calibration, integration, passport, appearance and report can be altered at will. A batch reprocessing queue is stored in a batch reprocessing queue file *.bar in the Data directory. 10.
10 Batch reprocessing 10.1.3 Save batch reprocessing queue QUEUE EDITOR / File / Save Save the batch reprocessing queue in a batch reprocessing queue file (*.bar) in the working directory. The batch reprocessing queue editor window remains open. QUEUE EDITOR / File / Save & exit Save the batch reprocessing queue in a batch reprocessing queue file (*.bar) in the working directory and close the batch reprocessing editor window. 10.2 Perform batch reprocessing 10.2.
10.2 Perform batch reprocessing Reprocess calibration runs Reprocess all calibration chromatograms (calibration level > 0). Update method file in directory after reprocessing Save the method file *.mtw after reprocess- ing if the method is changed. Reintegrate Reintegrate chromatograms according to the current settings of the integration parameters and integration events.
10 Batch reprocessing Default scheme switched on) by updating the concentration table. Default setting for recalibration reprocessing. The two options Apply final calibration... and Forget calibration points... are switched on. A new calibration is performed with the calibration runs and the resulting new calibration parameters (component table, concentration table and calibration curve) are applied to all sample runs.
10.2 Perform batch reprocessing Internal standard amount entered in the batch reprocessing table. The recalculation is done automatically if the Reintegrate and/or Recalculate options are enabled. If the Recalculate only option is enabled, the Reintegrate and Recalculate options are disabled automatically. Change passport If this option is enabled, those parameters of the passport which have been changed after clicking the button are applied to all chromatograms.
10 Batch reprocessing Send to Autodatabase Send chromatogram data to specified Autodatabase file. Open the Autodatabase options window for modification of the Autodatabase settings, see section 8.6.8. Start reprocessing. Combine all chromatograms of the batch reprocessing queue into a single multichannel chromatogram. Close the Reprocess window.
10.2 Perform batch reprocessing rel. concentration rel. concentration% peak amount Statistics Component Group Select the component which is to be evaluated statistically. It can be selected by clicking the pop-up list. default value: All Select the component group which is to be evaluated statistically. It can be selected by clicking the pop-up list. default value: All Open the Advanced window for export settings of the statistics.
10 Batch reprocessing Setting for the saving of the report file. Overwrite old report Write in new file (default) Ask before writing Path to viewer Type in the path of the Viewer program of the statistics output file or select it with the button. Open the Open window the MS Windows Explorer to select the viewer program for the statistics output file. recommended viewers: MS Excel, any ASCII editor e.g. MS Notepad The statistics output file is a ASCII file.
10.2 Perform batch reprocessing 10.2.2 Merge chromatograms REPROCESS / Combine all chromatograms of the batch reprocessing queue into a single multi-channel chromatogram. The chromatograms are displayed in the same order as in the batch reprocessing table slightly displaced one upon the other. The distance between the curves can be increased by pressing [ Shift ] + [ ↑ ] and decreased by pressing [ Shift ] + [ ↓ ]. The chromatogram axes, labels and colors can be set in the Appearance window.
10 Batch reprocessing 10.3 Batch reprocessing queue editor 10.3.1 Open batch reprocessing queue editor window REPROCESS / This menu item opens the batch reprocessing editor program for editing the batch reprocessing table. No File name Method Ident Vial Volume Dilution Amount Row number. Name of the chromatogram file (read-only). Name of the method file *.mtw used for recording the chromatogram (read-only). User defined identifier for chromatogram.
10.3 Batch reprocessing queue editor Sample Info 1 Sample Info 2 10.3.2 First sample description. Will be placed into appropriate passport field. Second sample description. Will be placed into appropriate passport field. Batch reprocessing queue editor functions The functions in the editor window for the batch reprocessing queues can be triggered with the corresponding menu items of the Edit menu or with the corresponding symbols in the symbol bar.
11 Appendix 11 Appendix 11.1 Software license The use of the Software is subject to this License Agreement between you and Metrohm AG. With the offer, you have received this License Agreement and taken note of it. You have already accepted this License Agreement upon the placement of your order with Metrohm AG or one of its distributors or upon confirmation of the order by Metrohm or one of its distributors.
11.1 Software license 5. Metrohm and its distributor disclaim all other warranties and liability. In particular, Metrohm and its distributor assume no liability for third-party damage or consequential damage, loss of data, lost profits, interruption of business, etc. 6. This Agreement applies until it is terminated. You can terminate this Agreement by destroying the Software and every copy of it.
11 Appendix 11.2 Declaration of conformity – Software validation The software "Metrodata IC Net 2.3" was developed in accordance with the requirements of the ISO 9001 quality system regarding the design, testing and servicing of Metrodata software. The relevant procedures are described in the document "Project procedure for creating Metrodata software" which is available at your Metrohm agency on request.
11.3 Index 11.3 Index % %B %C %D 135,137,140,142 135,137,140,142 135,137,140,142 ( (multi)Channel ratio 306 < 264 264 229 250 261 264 106 262 1 1st component 1st peak 280 280 2 21 CFR Part 11 21 CFR Part 11 On 25 25 7 709 IC Pump advanced features icon links settings window 732 IC Detector display features icon links method parameter program program instructions window 733 IC Separation Center features IC Net 2.
11 Anhang window 766 IC Sample Processor configuration features icon links manual control program program instructions status display window 771 Compact Interface data acquisition parameters features icon init links RS settings window 788 IC Filtration Sample Processor configuration features icon links manual control program program instructions status display window 793 Sample Prep Module features icon links manual settings program program instructions window 43 182 177 177 185 178 180 181 180 178 58 58 5
11.
11 Anhang Add> 47,48,55,56,72,74,80,81,86,88,117,122,132,1 37,142,146,149,152,155,158,161,164,168,17 1,181,183,184,185,190,192,193,194,216,226, 233,234,242,244,254,256,331 Adjusted volume 290 Administrator 18,19 Advanced 198 Advanced> 355 AIA 319,320 Air segment 202 Alarm buzzer 202 Alarm leak detector 92,247,258 Alarm stops 92,248,258 Alias 45,53,236,237 All 277,352 AlterGround 324 Amount 34,270,345,347,358 amount of substance 314 Analog output 96 Analog Outputs 260 Analysis number 270 Analysis time 208 Ap
11.
11 Anhang Coefficients 299 Colors 324 display settings 66 settings 324 system state window 66 watch window 66 Column 271,302 length 290 report 302 test results 308 Column heater 123 Column heating Deactivate 257 Column oven 251 Temperature 251 Column temp.
11.
11 Anhang ENABLED 47,55,72,79,86,116,122,132,146,149,152,155 ,158,168,242,254 END 48,55,87,243 End time 214 End> 197 Error messages 38 European Pharmacopoeia 280 Event 91,93,247,248,258,259,285 Events 47,55,284 Events configuration 48,56 Excel 336 configuration file 338 file 337 report 336 ExcelReport.ini 336 ExcelReport.
11.3 Index Group Groups 292,355 309 H Hair 324 halfwidth 311 Handshake 45,52,59 Hardware 90,246,249 Hardware error 92,93,247,248,258,259 Hardware requirements 5 Hardware? 258 Headspace pressure 202 height 311 Height 298 Height of theoretical plate 312 height% 311 Help 16 HETP/dp 312 Hide all> 323 High-pressure pump Program instruction 255 History 21 Home> 197 I IC Applications Collection (8.732.
11 Anhang Insert> 216 Installation 5 Integration algorithm 281 definition 10 events 284 parameters 282 reintegration 330 Integration parameters 281,351 Integration# 304 Intensity 263 Intensity spectrum 262 Interface 761 Compact IC 82 762 IC Interface 42 771 Compact Interface 58 830 IC Interface 49 844 UV/VIS Compact IC 249 861 Advanced Compact IC 238 add to system window 36 add to toolbar 28,40,41,64 definition 11 delete 42 install 40 Metrohm PC Board 60 new 40 Internal 280 Internal standard amount 34,271,3
11.
11 Anhang On-line help 16 Open 249 Open all files> 350 Open example> 350 Opening chromatograms 26 Operating hours 262 Ordering designations 362 Ordinary components 292 Other peaks 294 OUT 199 Output lines 225 Outputs 91,247 Oven 95,97,98,99,104,105 Overview file types 14 program windows 12 Overwrite old report 356 P Packing material 271 Page layout 328 Page margins 328 Paper size 329 Parameter 181,183,184,185,190,192,193,194,226,233,2 34,279 Parity 45,52,59 Particle size 271 Passport 268,353 Password 9,17
11.
11 Anhang calibration curve 305 component table 305 directory for report output 310 elements 311 file name 310 file output 307 font 17 integration 304 options 301 order of components 309 output 301 peak table 303,307 print 307,353 print preview 329 printing 329 results 303 screen output 307 statistics 353 system parameters 36 target 307 template 310 Report all peaks 309 Report date 302 Report destination 307 Report options 301,353 Represent raw data as 320 Reprocess 350 Reprocess calibration runs 351 Reproc
11.
11 Anhang file handling folder modify system window new open overwrite print system parameters save settings show system parameters system functions system state window system state window colors system window System file overwrite System settings System startup values System state System timer icon program System# 30 27,40,63 35 27 30 24 36 31 35 37 31 37 66 29 24 196,260 32,84,241 37 267 267 344,346 T T int 261 T out 261 t1 98,107 t2 98,107 t3 98,107 Tab size 310 Tabulated calibration 289,298 Task 181,1
11.3 Index Used User User administration User comments User List User Name User name> User program User program method UV lamp Deactivate UV Lamp UV_Lamp 298 19,269 17 25 18 17 330 206 215 251,262 257 253 255 display icon settings Wavelength Wavelength_1..3 What’s new while running save every ... min Width Will be executed by Wind.% Working directory Workplace definition Worst case...
