IC Equipment set Variocell for Bioscan – 6.5331.1X0 Manual 8.110.
Metrohm AG CH-9101 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com IC Equipment set Variocell for Bioscan – 6.5331.1X0 Manual 8.110.8009EN 10.
Teachware Metrohm AG CH-9101 Herisau teachware@metrohm.com This documentation is protected by copyright. All rights reserved. Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents Table of contents 1 Introduction 1.1 1 Description ............................................................................ 1 1.2 About the documentation ................................................... 1 1.2.1 Symbols and conventions ........................................................ 1 2 Components and connectors 3 3 Installation 5 3.1 Installing the Variocell ......................................................... 5 3.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures Table of figures Figure 1 Figure 2 IV ￭￭￭￭￭￭￭￭ Components and connectors of the Variocell .................................... 3 Remove the working electrode ..........................................................
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction 1 Introduction 1.1 Description The Variocell is an electrochemical flow cell with an exchangeable working electrode for usage in Bioscan instruments. The Variocell is constructed in such a way that both of the eluent outlets can serve as either inlets or outlets. The Variocell is available in four versions: ￭ ￭ ￭ ￭ Gold Variocell 6.1254.110 Glassy-Carbon Variocell 6.1254.120 Platinum Variocell 6.1254.130 Silver Variocell 6.1254.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.2 About the documentation Warning This symbol draws attention to a possible hazard due to electrical current. Warning This symbol draws attention to a possible hazard due to heat or hot instrument parts. Warning This symbol draws attention to a possible biological hazard. Caution This symbol draws attention to a possible damage of instruments or instrument parts. Note This symbol marks additional information and tips.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Components and connectors 2 Components and connectors Note The Variocell is supplied fully mounted. Immediately after receipt, check whether the shipment has arrived complete and without damage by comparing the delivery note with the list of accessories (see Chapter 6.1, page 15). 1 2 4 5 3 6 7 8 10 9 11 12 Figure 1 Components and connectors of the Variocell 1 Reference electrode connector For connecting the black 6.2156.000 electrode cable.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Reference electrode reservoir 6 Eluent inlet For connecting the connection capillary using one of the 6.2744.014 pressure screws provided. Can also be used as eluent outlet. 7 Eluent outlet For connecting the 6.1803.030 PTFE capillary using one of the 6.2744.014 pressure screws provided. 8 Clamping screw For adjusting the auxiliary electrode holder when inserting the working electrode. Can also be used as eluent inlet.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation 3 Installation 3.1 Installing the Variocell Caution Never switch the Variocell on, when... ￭ ￭ … it is not completely connected, or … if it is not simultaneously being rinsed by a conductive eluent. The working electrode or other electronic components may be damaged. Installing the Variocell 1 Removing the reference electrode Unscrew the reference electrode.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.2 Exchanging the auxiliary electrode ￭ Guide the free end of the drainage capillary into a sufficiently large waste container and fasten it there. 4 Inserting the reference electrode ￭ Switch on the high pressure pump of the chromatography system. This will cause the reservoir of the reference electrode (1-5) to fill up. Note The reservoir will overflow when the reference electrode is inserted. Keep paper towels close to hand.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation Dismantling the Variocell 1 Dismantling the Variocell ￭ ￭ ￭ ￭ In the software, switch off the Variocell and the high pressure pump. Dismantle all three electrode cables. Remove the Variocell from the holder in the instrument. Unscrew the eluent inlet and eluent outlet. 2 Removing the auxiliary electrode holder ￭ ￭ Unscrew the fastening ring (1-11) on the working electrode holder and remove it. Carefully pull the working electrode holder (1-10) out of the Variocell.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3.2 Exchanging the auxiliary electrode 2 Inserting new working electrode ￭ ￭ ￭ Insert new working electrode (1-9) into the silicone holder (2-2) of the working electrode holder (1-10) with the polished side facing outward. Remove fingerprints with acetone or methanol. Secure the working electrode with the mounting ring (2-1).
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Maintenance - Taking out of operation 4 Maintenance - Taking out of operation In principle, the Variocell is maintenance-free. Contamination appearing after prolonged utilization could however influence the measuring result. If the measuring signal should exhibit excessive noise, then you can quite easily remove the Variocell and clean it. To do so, proceed in accordance with the following instructions: 4.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.1 Maintaining the Variocell Cleaning the components of the Variocell 1 Cleaning the spacer Remove the spacer. Rinse with ultra pure water and dry. ￭ ￭ 2 Clean the auxiliary electrode Carefully clean the auxiliary electrode (1-3) with a soft cloth soaked with acetone or methanol. Avoid any use of force while doing so; the electrode could become damaged. Rinse off the interior side of the cell with ultra pure water and dry thoroughly.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Maintenance - Taking out of operation ￭ ￭ Insert spacer. Screw together the two pieces of the Variocell. 2 Inserting the working electrode Note Working electrodes have a front side and a rear side. The front side is polished smooth and glossy, while the rear side is more matte. "Au", "Ag" or "Pt" is also engraved on the rear side of working electrode plates made of gold, silver or platinum, respectively.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.1 General 5 Technical specifications 5.1 General Structure Flow cell with working, reference and auxiliary electrode. Cell volume 0.7 µL with the supplied 50 µm spacer. Working temperature The cell should not be operated continuously over 45 °C. Reference electrode Type Solid phase reference electrode Conversion to Ag/AgCl E Hy-REF /mV = E Ag/AgCl /mV – 328 + 29.9 pH (mV) 5.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.3 5 Technical specifications Glassy-Carbon Variocell Working electrode Glassy-Carbon Material Aromatics and amines ￭ Catecholamines, aromatic amines ￭ Inorganic ions (nitrite, sulfite, ...) ￭ Phenols ￭ Vitamins ￭ Some amino acids Applications Working area Acid medium -0.8 V… +1.3 V Alkaline medium -1.5 V… +0.6 V 5.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.5 Silver Variocell 5.5 Silver Variocell Working electrode Material Applications Silver Applications relevant to the environment Halogenides ￭ Cyanide, sulfide ￭ Thiosulphate ￭ Pharmaceutics ￭ Working area Acid medium -0.55 V… +0.4 V Alkaline medium -1.2 V… +0.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6 Accessories 6 Accessories 6.1 Scope of delivery 6.1.1 Equipment with Au Variocell for Bioscan 6.5331.110 Qty. 1 IC Equipment set Variocell for Bioscan Order no. Description 6.1254.110 Gold Variocell for Bioscan Gold measuring cell with exchangeable working electrode. 1 6.1254.410 Spacer washer for Variocell, 50 µm 50 µm spacer washer for 6.1254.1X0 Variocell 1 6.1803.030 PTFE capillary 0.5 mm i.d.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Scope of delivery Qty. 1 Order no. Description 6.2744.014 Pressure screw 2x With UNF 10/32 connection. For the connection of PEEK capillaries Material: PEEK Length (mm): 26 1 6.1.2 8.110.8009DE Equipment with GC Variocell for Bioscan 6.5331.120 Qty. 1 Manual for 6.5331.1X0 equipment with Variocell for Bioscan, English IC Equipment set Variocell for Bioscan Order no. Description 6.1254.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 6 Accessories Order no. Description 6.1254.410 Spacer washer for Variocell, 50 µm 50 µm spacer washer for 6.1254.1X0 Variocell 1 6.1803.030 PTFE capillary 0.5 mm i.d. / 3 m Capillary for Inline Dialysis, for Dialysis Unit, IC Dialysis Sample Processor, IC Liquid Handling Unit Dialysis Material: PTFE Outer diameter (inches): 1/16 Inner diameter (mm): 0.5 Length (m): 3 1 6.2744.014 Pressure screw 2x With UNF 10/32 connection.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Scope of delivery 6.1.3 Equipment with Pt Variocell for Bioscan 6.5331.130 Qty. 1 IC Equipment set Variocell for Bioscan Order no. Description 6.1254.130 Platinum Variocell for Bioscan Platinum measuring cell with exchangeable working electrode. 1 6.1254.410 Spacer washer for Variocell, 50 µm 50 µm spacer washer for 6.1254.1X0 Variocell 1 6.1803.030 PTFE capillary 0.5 mm i.d.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Qty. 1 6 Accessories Order no. Description 6.2744.014 Pressure screw 2x With UNF 10/32 connection. For the connection of PEEK capillaries Material: PEEK Length (mm): 26 1 6.1.4 8.110.8009DE Equipment with Ag Variocell for Bioscan 6.5331.140 Qty. 1 Manual for 6.5331.1X0 equipment with Variocell for Bioscan, English IC Equipment set Variocell for Bioscan Order no. Description 6.1254.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6.1 Scope of delivery Qty. 1 Order no. Description 6.1254.410 Spacer washer for Variocell, 50 µm 50 µm spacer washer for 6.1254.1X0 Variocell 1 6.1803.030 PTFE capillary 0.5 mm i.d. / 3 m Capillary for Inline Dialysis, for Dialysis Unit, IC Dialysis Sample Processor, IC Liquid Handling Unit Dialysis Material: PTFE Outer diameter (inches): 1/16 Inner diameter (mm): 0.5 Length (m): 3 1 6.2744.014 Pressure screw 2x With UNF 10/32 connection.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Index A Assembly .................................. 10 Auxiliary electrode ...................... 3 Holder .................................. 4 C Cleaning ................................... 10 Auxiliary electrode .............. 10 Spacer ................................ 10 Working electrode .............. 10 D Dismantling ............................ 7, 9 E Electrode Exchanging ...........................
