Instruction manual

Section 5

Buffer Temperature

5.1 Temperature Controller

The temperature controller maintains the desired buffer temperature in the D GENE

system (Figure 5.1). The actual and set buffer temperatures are displayed in °C. The set

temperature can be adjusted by using the raise and lower buttons. The maximum buffer

temperature that can be set is 65 °C. The heater light will come on when the heater is on.

Fig. 5.1. The Temperature Controller displays the actual and set temperatures.

5.2 Preheating the Running Buffer

1. Pour 1x TAE buffer into the D GENE chamber up to the Fill line. It takes approximately

7 liters of 1x TAE buffer to fill the chamber.

2. Place the D GENE lid onto the chamber. Attach the power cord. Turn the power and

heater switch on. The loading lid should be on the D GENE lid during preheating.

3. Set the temperature 2–4 °C above the desired temperature. During sample loading the

buffer temperature can drop 2–4 °C.

4. It can take about 1 to 1.5 hours for the D GENE system to heat the buffer up to the desired

temperature (56–60 °C). Heating the buffer in a microwave can reduce the time it takes

to get to the desired temperature.

ACTUAL

SET

HEATER