Instruction manual

7. Remove the alignment card. Remove the sandwich assembly from the casting stand and

check that the plates and spacers are flush at the bottom. If the spacers and glass plates are

not flushed, realign the sandwich and spacers to obtain a good seal (Repeat steps 5-7).

8. Once a good alignment and seal are obtained, tighten the clamp screw until it is finger-

tight. Do not over tighten, plates may crack.

9. For assembling a 16 x 16 cm or 16 x 10 cm CDGE or parallel gel, go to step 16. If you

are assembling a 16 x 16 cm or 7.5 x 10 cm perpendicular gradient gel, go to step 10.

10. Place the proper comb in the sandwich and set it against the special notches provided on

the spacers. For the 7.5 x 10 cm perpendicular gel, insert the middle spacer on the center

of the sandwich until it touches the middle notch on the comb and straighten the spacer.

The bottom of the middle spacer should also be flushed against the glass plates without

any snags (Figure 6.4).

Note: If casting a single 7.5 x 10 cm gel, use the filler spacer, which fills the glass plate

on the side that is not being used.

Fig. 6.4. Positioning the middle spacer in the sandwich assembly.

11. Set the sandwich standing upright on a flat surface. Loosen the comb gasket screws

(located on the back, Figure 6.5). Mark a straight line on the middle of the screw head

using a permanent marker (this will be the marker for the turns needed for the screws).

Carefully place the comb gasket on top of the comb with the screws facing towards the

large plate. Position the comb gasket so that the notched steps at the top of both ends of

the soft part of the comb gasket is against the notches at the top of the spacers. Twist the

screws until it makes contact with the glass plate. Twist the screws an additional

⁄4 turn.