Instruction manual

Note: The lid can be attached to the buffer chamber in only one orientation, so that the

anode and cathode connections cannot be reversed.

4. Remove the D GENE loading lid from the D GENE lid.

5. Load the samples into the wells using a pipet and a sequencing loading tip. Be careful not

to not pierce the gel during sample delivery. A multichannel pipet with 8 tips can be used.

Note: The loading volume for the single well comb that is used with the perpendicular gels

is about 200–400 µl.

6. Place the D GENE loading lid back onto the D GENE lid. Turn the pump on. Adjust to

the correct temperature.

7.3 Running the Gel

1. Attach the electrical leads to a suitable DC power supply with the proper polarity

(this connection could accidentally be reversed).

2. Apply power to the D GENE system and begin electrophoresis. You can also run the

samples into the gel before turning on the pump. As a safety precaution always set

voltage, current, and power limits when possible.

7.4 Removing the Gel

1. After electrophoresis is complete, turn the power supply and D GENE system (heater,

pump, and main power) off. Disconnect the power cord and electrical leads. Let the heater

cool for about 15 seconds in the buffer.

2. Remove the D GENE electrophoresis system and place it on the holder. CAUTION: The

heater is still hot. Do not touch. Carefully pull the core out of the lower buffer chamber.

Pour off the upper buffer into the lower buffer chamber by tilting the core into the chamber.

3. Lay the core and gel sandwiches on a padded surface.

a. For 16 x 10 cm and 7.5 x 10 cm gels, remove the sandwich assembly with your index

fingers on the sandwich clamps and your thumbs resting on the latches on the core.

Gently remove the assembly by pushing the latch away from the gel sandwich. Pry the

sandwich upward and pull (Figure 7.3).

b. For 16 x 16 cm gels, remove the sandwich assembly with your index fingers below the

sandwich clamps and your thumbs resting on the latches on the core. Gently remove

the assembly by pulling up toward you (in a manner opposite to the way it was

attached). Pull the sandwich assembly off the locating pins on the top of the core.