Instruction manual

Section 1

Equipment Overview

1.1 Safety

Read the manual before using the D GENE system. For technical assistance, contact your

local Bio-Rad Office or, in the U.S., call technical services at 1-800-4BIORAD (1-800-424-

6723).

This instrument is intended for laboratory use only.

This product conforms to the “Class A” standards for electromagnetic emissions intend-

ed for laboratory equipment applications. It is possible that emissions from this product may

interfer with some sensitive appliances when placed nearby or in the same circuit as thise

appliances. The user should be aware of this potential and take asppropriate measures to avoid

interference.

DC power to the D GENE system is supplied by an external DC voltage power supply.

This power supply must be ground isolated so that the DC voltage output floats with

respect to ground. All of Bio-Rad’s power supplies meet this important safety requirement.

Regardless of which power supply is used, the maximum specified operating parameters for

the system are

Maximum voltage limit 300 VDC

Maximum power limit 50 watts

AC current for controlling temperature to the system, and DC current for electrophoresis,

provided from the external power supply, enter the unit through the lid assembly, which pro-

vides a safety interlock. DC current to the cell is broken when the lid is removed. Do not

attempt to circumvent this safety interlock. Always disconnect the AC cord from the

unit and the coiled cord from the DC power supply before removing the lid, or when

working with the cell.

Definition of Symbols

Caution, risk of electric shock

Caution (refer to accompanying documents)

Important precautions during set up

1. Always inspect D GENE system and replace any damaged components before use.

2. Place the D GENE system on a steady, level bench top.

3. Do not use near flammable materials.

4. Do not use buffers which are not compatible with construction material of the system.

5. Always place the lid assembly on the buffer tank with the AC and DC power cords dis-

connected.

6. Always connect the system to correct the AC and DC power sources.

Important precautions during the run

1. Always add buffer to the “Fill” line on the tank when preheating; always keep the buffer

below “Max” level during electrophoresis.