Instruction manual

the core to provide a greaseless, leak-free seal for the upper buffer. Each sandwich forms one
side of the cathode chamber. If only one gel is to be run, use a set of glass plates without a spac-
er to form a buffer dam sandwich. In addition, the core has built-in passage for upper and
lower buffer circulation by the pump.
4. Comb Gasket Holder
The comb gasket holder seals the top edge of the glass sandwich for casting perpendicu-
lar gradient gels. Align the comb gasket so that the notched steps on both ends of the soft part
of the comb gasket is against the notches at the top of the two spacers. A proper alignment is
required for a good seal.
5. Sandwich Clamps
The patented sandwich clamps consist of a single screw mechanism which makes assem-
bly, alignment, and disassembly of the gel sandwich an effortless task. The clamps exert an
even pressure over the entire length of the glass plates. Each pair of clamps consists of a left
clamp and a right clamp. Each sandwich can hold a gel up to 1.5 mm thick.
6. Casting Stand with Sponge
The casting stand is separated from the cell so that gels can be prepared for the next run
while others are running.
7. Stopcock and Air Vent Plugs
Stopcocks are used at the inlet ports when casting a gradient perpendicular gel. The air vent
plugs are used in the comb gasket to close the gel sandwich after casting.
8. Prep Comb and Spacer Set
The prep comb works with spacer sets to form a single sample well or dual sample wells
per gel.
9. Alignment Card
The Alignment Card simplifies sandwich assembly by keeping the spacers upright dur-
ing sandwich alignment.
10. Model 475 Gradient Delivery System
The Model 475 Gradient Delivery System is an innovative cam-driven module for form-
ing accurate, reproducible gradient gels.
11. Filler spacer (optional)
Allows casting of single mini-gel.
12. Pressure Clamp (not shown in photo)
The pressure clamp provides equal pressure to the comb gasket for a good seal.
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