www.milwaukeetesters.com INSTRUCTION MANUAL Milwaukee W ine LLab ab Minititrator Wine Mi455 Free & TTotal otal Sulphur Dioxide www.milwaukeetesters.
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis TABLE OF CONTENTS FUNCTIONAL DESCRIPTION ............................................................................... 3 GENERAL DESCRIPTION ..................................................................................... 4 PRINCIPLE OF OPERATION .................................................................................. 6 SPECIFICATIONS ........................................................................
www.milwaukeetesters.com FUNCTIONAL DESCRIPTION DISPLAY A. HOURGLASS ICON B. BATTERY STATUS ICON C. ERROR MESSAGE D. MEASURE STATUS E. CALIBRATION MODE INDICATOR F. MAIN DISPLAY G. MEASUREMENT UNIT H. TIMER MODE INDICATOR I. SECONDARY DISPLAY FRONT PANEL A. TITRANT BOTTLE B. LIQUID CRYSTAL DISPLAY (LCD) C. KEYPAD D. ELECTRODE HOLDER REAR PANEL E. ORP ELECTRODE F. PERISTALTIC PUMP TUBE G. BEAKER H. PERISTALTIC PUMP I. BNC ELECTRODE CONNECTOR J. FUSE K. POWER CABLE CONNECTOR L.
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis KEYPAD A. PUMP - to start/stop the pump B. CAL - to enter pump calibration mode C. STIR - to start/stop the stirrer while in measurement or purging mode D. START STOP - to start/stop titration or pump calibration GENERAL DESCRIPTION The Mi455 is a low-cost, easy to use, microprocessor-based automatic titrator. It has a simple and reliable peristaltic pump that ensure high dosing repeatability.
www.milwaukeetesters.com “bisulphite” (HSO3-). The smaller part of the free is the active molecular SO2. The amount of molecular SO2 in your wine depends both on the level of free SO2 present as well as pH. For instance, at pH 3.2, the amount of free SO2 for 0.8 ppm molecular SO2 is 22 ppm. At pH 3.5, you will need 43 ppm free - essentially double. Free SO2 concentration (ppm) for 0.8 ppm molecular SO2: pH 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis PRINCIPLE OF OPERATION Determination of sulphur dioxide in wine samples is made by titration of the sulphur dioxide present in wine with iodate. In this procedure an excess of iodine is added to the wine sample and it is titrated with iodate. For precise results it is very important to know the exact sample volume, titrant volume and concentration.
www.milwaukeetesters.com SPECIFICATIONS Range Resolution Accuracy Method Principle Sample volume ORP Electrode Pump debit Stirring speed Environment Power supply Dimensions Weight 0 to 400 ppm SO2 1 ppm 5% of reading Ripper titrimetric method Equivalence point redox titration 50 mL MA924B/1 (included) 0.5 mL/min 1500 rpm 0 to 50 °C (32 to 122 °F) ; max 95% RH non-condensing 220V/50Hz; 10VA 208 x 214 x 163 mm (8.2 x 8.4 x 6.4") (with beaker) 2200 g (77,6 oz.).
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis STARTUP • Place the instrument on a flat table. Do not place the instrument on direct sun light. • Connect the titrator to mains socket with ground connection and the correct voltage and frequency. See the label on the instrument rear for this. • Place the peristaltic pump tube on the pump. See the Pump Tube Replacement section for the procedure. • Remove the reagent bottle cap and place the bottle cap of the tubes set.
www.milwaukeetesters.com PROBEGUIDE FUNCTIONAL TO DISPLAYDESCRIPTION CODES This screen appears for a few seconds each time the instrument is turned ON. Main screen display. Main screen display with stirrer active. Purging mode message. PUMP CALIBRATION MESSAGES This screen appears each time the meter enters pump calibration mode. The meter is ready to start pump calibration by pressing the START STOP button. This screen appears while pump calibration is in progress.
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis This error message appears when the sample concentration exceeds 400 ppm. The used standard solution is wrong. This error message appears when the input readings (mV) exceed the input limits (0÷1000mV). TITRATION MESSAGES This screen appears each time a titration is running. When the START STOP button is pressed the minititrator returns to the main screen.
www.milwaukeetesters.com GENERAL TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be carefully followed during testing to ensure best accuracy. • Purge the peristaltic pump to have fresh titrant when starting a new analysis or calibration. • Calibrate the peristaltic pump before performing an analysis. • Analyze the wine immediately after the sample is obtained.
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis • Open refill cap on the ORP electrode and immerse the electrode approximately 2 cm (0.8”) into the sample to be tested. Do not touch stir bar with the tip of the electrode. • Ensure there is titrant in the dosing tip. If not, hold the dosing tip over an empty container and press PUMP for a few seconds until the titrant is in the dosing tip. • Add the contents of one powder packet of Mi555-006 Stabilizer into the beaker.
www.milwaukeetesters.com FREE SO2 MEASUREMENT PROCEDURE • Using a clean pipette, fill the 50 mL beaker up to 50 mL mark with the wine sample. Note: It is important to be accurate in your measurements of Wine sample. Pipettes are recommended. • Place the stir bar in the beaker and put the beaker in the minititrator top. • Fill the 20 mL beaker up to the 5 mL mark with the Mi555-005 Acid Reagent and add the content to the 50 mL beaker.
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis • Press START STOP in order to start titration. The display will show “----” during titration, along with stirrer and pump tags blinking on the LCD. • At the end of the titration, the Sulphur Dioxide concentration is displayed in mg/L (ppm). TOTAL SO2 MEASUREMENT PROCEDURE • Fill the 50 mL beaker up to 50 mL mark with the wine sample. Note: It is important to be accurate in your measurement of wine sample.
www.milwaukeetesters.com • Insert dosing tip into holder. Ensure there is titrant in the dosing tip. If not, hold the dosing tip over an empty container and press PUMP for a few seconds until the titrant is in the dosing tip. The tip should be below the pump to ensure gravity flow and minimize the chance of air bubbles in the tube. • Add the content of one powder packet of Mi555-006 Stabilizer into the beaker. • Press START STOP button to start titration.
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis FUSE REPLACEMENT To change the fuse follow next steps: • Disconnect the power cord from the rear panel of the instrument. • Pull out the fuse holder located near the power cord connector. • Replace the fuse with a similar one. • Push the fuse holder with the fuse in the appropriate place. ELECTRODE CONDITIONING & MAITENANCE PREPARATION PROCEDURE Remove the protective cap of the ORP electrode (MA924B/1).
www.milwaukeetesters.com than 2½ cm (1”) below the fill hole, add MA9011 3.5M KCl Electrolyte Solution. For faster response, unscrew the fill hole screw during measurements. STORAGE PROCEDURE To minimize clogging and assure a quick response time, the glass bulb and the junction of the electrode should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of MA9015 Storage Solution or, in its absence, Filling Solution (MA9011).
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis ACCESSORIES REAGENT SETS MA9011 Electrode filling solution (230 mL) MA9015 Electrode storage solution (220 mL) MA9016 Cleaning solution (220 mL) Mi555-001 Calibration standard (500 mL) Mi555-002 Titrant solution (100 mL) Mi555-003 Alkaline reagent (100 mL) Mi555-004 Acid reagent for Total SO2 determination (100 mL) Mi555-005 Acid reagent for Free SO2 determination (100 mL) Mi555-006 Stabilizer reagent (40
www.milwaukeetesters.com For your Safety don’t use or store the instrument in hazardous environments. To avoid damages or burns, do not perform any measurement in microwave ovens. WARRANTY This instrument is warranted against defects in materials and manufacturing for a period of 2 years from the date of purchase. Electrodes are warranted for 6 months.
Instruction Manual Mi455 FREE & TOTAL SULPHUR DIOXIDE Minititrator for wine analysis THANK YOU FOR CHOOSING Sales and Technical Service Contacts: Milwaukee s.r.l. C.so Leonardo da Vinci 48/50 21013 Gallarate (VA) ITALY Tel: +39 0331 26 80 09 Fax: +39 0331 26 80 33 e-mail: sales@milwaukee.191.it Milwaukee Instruments, Inc. 2950 Business Park Drive Rocky Mount, NC 27804 USA Tel: +1 252 443 3630 Fax: +1 252 443 1937 e-mail: milwaukee@vol.com www.milwaukeetesters.
