User's Guide
7 • Miro Canvas • User Guide
2. PROCEDURE
• Tube Recommendations
The table below shows tubes that are known to be both compatible and incompatible with Miro Cartridge.
This list is not comprehensive.
• Reagent Preparation
i. i. Prepare reagents as required for desired workow
ii. Keep Dropgloss at room temperature (19-25°C). Vortex Dropgloss well in the original container before use.
Dropgloss will solidify below 18°C. If this occurs, hold the tube in your hand until completely thawed,
then vortex well. Visually inspect the Dropgloss in your pipette tip to ensure that it has completely
thawed before adding to the reaction.
Dropgloss is light-sensitive and may also react with alternative storage plastics, resulting in
diminished performance; it should never be aliquoted.
iii. If desired, shear input DNA in an appropriate buffer (e.g. 10 mM Tris-HCl).
iv. Barrier pipette tips should be used when preparing, loading, and recovering reagents and samples.
v. Assemble reaction mixes in separate 0.2 ml PCR tubes:
1. Add reagent(s) to each tube, then add Dropgloss to required reagents. Dropgloss will naturally
separate from reagents in the tube.
a. Dropgloss will solidify below 18°C.
While original enzyme stocks should
be kept on ice, reaction mixes should
be kept at room temperature on the
benchtop until you are ready to load
the cartridge. Do NOT keep reaction
mixes on ice.
Compatible
N8010580
PCR-02-C
86403
87003-294
T320-3N
Axygen
Axygen
Eppendorf NA
VWR
Incompatible
MicroAmp 8-Tube Strip; 0.2ml
Maxymum Recovery PCR Tube; 0.2ml
Microcentrifuge Tubes, Polypropylene; 1.7ml
Amplitube PCR Reaction Strips, 8x0.2 ml
CAT. NO.SUPPLIER PRODUCT DESCRIPTION