Technical Publication No. 71100-852 11 /9Ö OPERATING MANUAL FOR MISTRAL 10OO & 2 0 0 0 CENTRIFUGES PLEASE READ CAREFULLY MSE Bishop Meadow Road, Loughborough, Leicestershire, LE11 ORG England.
Contents Section 1 2 3 4 5 6 7 8 9 10 11 12 13 14 General Description Important Information for Good Operator Practice Installing the Centrifuge Rotors and Accessories - Fitting the Rotor - Loading the Rotor - Balancing the Rotor - Removing the Rotor How to operate your Mistral 1000 and 2000 Centrifuges Opening the Lid Parameter Entry Starting the Run Error Conditions Maintenance Specifications Explanation of Error Messages Rotors and Accessories Service Instructions Page 1 2 3 6 6 6 6 6 7 8 9 11 12 13 2
Thank you for buying an MSE centrifuge. Please read the enclosed operating manual before using your centrifuge; it will provide you and your colleagues with useful information on all aspects of the centrifuge and its accessories. As our customer, we should like to ensure that you are totally satisfied at all times. Do not hesitate to contact MSE - your views are very important to us.
1. General Description The MISTRAL 1000 and 2000 high performance bench top centrifuges join the well established range of MSE MISTRAL centrifuges. The MISTRAL range now consists of: MISTRAL 1000 MISTRAL 2000 MISTRAL 3000i MISTRAL 3000E MISTRAL 6000 The entire MISTRAL range of centrifuges has been designed to meet the present and future demands of routine and research laboratories.
2. Important Information for Good Operator Practice Spillages In the event of liquid spillage, the affected surfaces should be cleaned immediately. The rotor and accessories should be removed and, if necessary, the inner bowl should be removed for thorough cleaning, (see Section 10). Regular cleaning of the centrifuge is highly recommended to avoid the build up of contamination. Materials with specific gravity in excess of 1.
3. Installing the Centrifuge IMPORTANT: THE ROTOR MUST NOT BE FITTED WHEN MOVING OR DURING TRANSIT Preparation Remove the centrifuge from its packing and place it on a rigid Ievel surface. The location of the control panel, lid lock access hole, mains power switch and mains fuses are shown in Fig. 1A. Check that the available power supply corresponds to that stated on the rating plate located at the rear of the instrument, (see Fig. 1B).
COOLING AIR INLET SLOTS IN LID (DO NOT OBSTRUCT) CONTROL PANEL LID LOCK OVERRIDE ACCESS HOLE (SEE PAGE 12) MAINS POWER SWITCH FIG.1A. MAINS FUSES SEE FIG. 13 FOR DETAILS PLUG COVERING LID FIXING SCREWS RATING PLATE & SERIAL NUMBER MAINS CABLE FIG.1B. COOLING AIR EXIT SLOTS (DO NOT OBSTRUCT) Fig. 1AandFig.
/ / / S / / / / / / / / / S / / / / / / / / / S / / / / / / / / / / / / / / S / / / / / / / / S . 75 mm 75 mm Fig. 2. MINIMUM OPERATING CLEARANCES A TEMPLATE IS SUPPLIED WITH THE BRACKETS TO AID ACCURATE POSITIONING ON THE BENCH Fig.
4. Rotors and Accessories Tables 3 to 13 on pages 24 to 34, give details of the range of rotors and accessories available for use on the Mistral 1000/2000 centrifuges. The drive head design and rotor identification system is common to both instruments.,The rotors are therefore interchangeable between the models, with the following exceptions: IMPORTANT: - The 4 x 200 rotor (cat no 43124-134) and Microtitration rotor (cat no 43121 -117) MUST NOT BE FITTED TO THE MISTRAL 1000.
5. How to Operate your Mistral 1000 and 2000 Centrifuges Switching on The key pad Connect to the power supply. Press the POWER ON switch at the front of the instrument, (0 = OFF, 1= ON). The switch will light up and you will hear an audible signal. LIQUID CRYSTAL DISPLAY NUMERIC KEYS L CANCEL ENTRY KEY L MULTIFUNCTIONAL KEYS STOP KEY _ START KEY Fig.4 CONTROL PANEL AND DISPLAY Understandinq the The liquid crystal display shows two lines of information. (See Fig.
6. Opening the Lid Switching on Connect the power supply and press the POWER ON switch. The POWER ON switch is illuminated green when activated. The liquid crystal display will become active and an audible signal informs the user that the instrument is ready for use. The display will settle to that shown in Fig. 5. The instrument is equipped with battery back-up and, on power up, will display the values current when the instrument was last switched off.
7. Parameter Entry Upon closing the lid, the display will revert to that shown in Fig. 5. To start the machine using the current parameters shown on the top display line, press <]> (START). To alter the parameters press RHK A indicated by 'ALTER' on the prompt line. The display will change to that shown in either Fig. 7 or Fig. 8. The operator can now enter speed or RCF(g). To move between speed and RCF(g) press the LHK • indicated by 'RPM/G' on the prompt line.
Entering run time The display will change to that shown in Fig. 9. SETTII1E10niIlUTES tTME/HOLD IÏEXT+ Fig. 9 The current run time is displayed and may be altered within the following ranges: 0-9.9 minutes and 10-99 minutes, using the numeric key pad. During the run, the time remaining will be displayed. If time hold is required, press the LHK A indicated by the prompt 'TIME/HOLD'. In this case, the time elapsed from the start of the run is indicated.
8. Starting the Run Ensure that the rotor is correctly loaded and fitted. Close the lid and press down firmly to engage the lid lock. Press (START). The display will now change to show actual values of speed and run time. After a short delay the motor will engage. Fig. 11 shows a typical display during the run. The run status of the machine is indicated by the messages RUNNING or AT SPEED or STOPPING. This is displayed intermittantly with the SHOW SET message. 9
9, Error Conditions Error m e s s a g e s Any error message is intermittentiy displayed on the bottom line of the display, as indicated in Fig. 12. The display is accompanied by an audible alarm. 32 runs 11/C HRS ItlBRLRtiCED Fig. 12 Please refer to Table 2 on page 21 for a full explanation of each message and the correct course of action.
10. Maintenance This section describes the basic maintenance procedures, in particular, the methods and materials used for cleaning the centrifuge, rotors and accessories. Cleanliness To maintain a good appearance and to prevent dirt build-up, the casing and the inside of the bowl shouid be cleaned regularly using a soft cloth dampened with a neutral detergent and warm water. The key pad and display may also be cleaned in this manner, but shouid be wiped dry immediately.
Sterilisation All of the rotors, buckets and sealing caps (with O-Rings removed) and the bowl may be sterilised by autoclaving at 120°C, (see page 16 for bowl removal procedure). If the means available for disinfection of certain microbiological agents are inadequate, the safety officer should be consulted and appropriate steps taken. Lid seal Lightly coat the lid seal with French chalk occasionally and in particular after cleaning.
MAINS FUSE HOLDERS SEE TABLE 1 FOR CORRECT FUSE RATING PULL INSTRUMENT TOWARDS FRONT OF THE BENCH Fig. 13 MAINS FUSE ACCESS AND REPLACEMENT LID SEAL PUSH DOWN AT BASE BOWL MOULDED CASE CORRECT POSITION OF LID SEAL Fig.
Removing and re-fitting the bowl TO BE CARRIED OUT BY QUALIFIED PERSONNEL ONLY. _ #. . ., . . . ... .. . . .. To remove the bowl for cleaning or stenlisation, proceed as follows: (i) Open the lid, disconnect the power supply and remove the rotor assembly. (ii) Turn the machine around and remove the four hole-blanking plugs covering the lid fixing screws, (see Fig. 1B). With the lid fully open (vertical), remove the screws using the M5 Allen key provided and lift the lid assembly clear.
Replacing the motorbrushes TO BE CARRIED OUT BY QUALIFIED PERSONNEL ONLY. ... _ (i) Open the lid, disconnect the power supply and remove the rotor assembly. Remove the bowl as described earlier, (see page 16). t. ... .. .t. . (ii) Pinch the top section of the rubber motor gaiter and pull up and over the plastic moulding. iU NB - Do not pull the gaiter up more than is necessary to gain access with a 'Posidrive' screwdriver as shown in Fig. 15A.
Fig. 15A REMOVING THE MOTOR ASSEMBLY PIGTAIL MUST BE COILED BEHIND SPRING BRUSH UNIT HOLDER BRUSH SPRING MISTRAL 1000 - 120V MISTRAL 1000 - 220/240V MISTRAL 2000 BRUSH ASSEMBLIES Fig.
It is recommended that a regular inspection is made (after each 250 hours of operation time or every 6 months, whichever is the sooner) of the centrifuge, inciuding rotors and accessories, to ensure reliability and safety. For service information contact: (Under guarantee): Sanyo Gallenkamp PLC, Technical Sales Support, Bishop Meadow Road, Loughborough, Leicestershire. LE11 ORG Tel: (0509) 237371 (Out of guarantee): Fisons Instruments, Sussex Manor Park, Gatwick Road, CRAWLEY, West Sussex. RH10 2QQ England.
11. Specifications MISTRAL 1000 MISTRAL 2000 Power consumption at full load, (Acceleration Power shown in brackets) 120V 50/60Hz single phase 220V 50/60Hz single phase 240V 50/60Hz single phase 120V- 550 watts (1000) 600 watts (1000) 220V - 650 watts (1100) 700 watts (1300) 240V - 600 watts (1000) 700 watts (1300) Speed range 200-6000 rpm 200-6500rpm RCF range 5-4750 5-5575 Power Supply options Speed control accuracy ± 10 rpm Speed display ± 25 rpm Timing accuracy 0.
12. Explanation of Error Messages TABLE2 Explanation of Error Messages Note 1: In the event that the lid cannot be opened electronically, open it manuaily (see page 12), power up and press CE. Note 2:lf an error is not rectified by taking the suggested action, or if it is erratic or persists without apparent cause, disconnect the power supply and contact the service engineer. ERROR MESSAGE EXPLANATION EFFECT ON MACHINE OPERATION Data loss Data loss Machine will not run or will stop.
TABLE 2 (continued) ERROR MESSAGE EXPLANATION EFFECT ON MACHINE OPERATION OPERATOR ACTION Tacho signal loss No speed signal feedback. Machine will stop. 1. Wait for 3 minutes or until you are sure the rotor has stopped turning. Follow "Emergency Lid Release" procedure on page 12 to open the lid. (SEE NQTE 1). Lid Lock disabled preventing normal lid operation. Tacho signal erratic 2. Check that a rotor is fittéd. Fault in tacho signal. 3.
TABLE 2 (continued) ERROR MESSAGE EXPLANATION EFFECT ON MACHINE OPERATION OPERATOR ACTION Loss of Drive Speed is reducing at a time when the control system is calling for acceleration or constant speed. Machine will stop. 1. Check that the cooling airflow is not obstructed. (Refer to figures 1A, 1B and 2). A qualified person should check that the motor gaiter is fitted correctly. Allow motor to cool before re-starting the instrument (SEE NOTE 1). Lid Lock disabled preventing normal lid operation.
TABLE 3 4 x 100 SWING OUT ROTOR (MSE 43121-302).3600 R.P.M. (MAX SPEED) ACCESSORIES 100ml TRUNNION (34134-611), 100ml SHORT BUCKET (34411 -912) SEALED CAP ASSEM BLY (MSE 43561 -607. CLEAR PLASTIC) SEALED BUCKET ADAPTOR TUBE CAPACITY COLOUR CAP RADIUS (MM) CAT. NO.
TABLE 4 4 x 100 SWING OUT ROTOR (MSE 43121-302).3600 R.P.M. (MAX SPEED) ACCESSORIES 10Oml TRUNNION (34134-611), 100ML SHORT BUCKET (34411 -912) SEALED CAP ASSEMBLY (MSE 43561-607. CLEAR PLASTIC) SEALED BUCKET ADAPTOR TUBE CAPACITY COLOUR CAP CAT. NO.
TABLE 5 4 x 100 SWING OUT ROTOR (MSE 43121-302).3600 R.P.M. (MAX SPEED) ACCESSORIES 10Oml TRUNNION (34134-611), 10OML SHORT BUCKET (34411 -912) SEALED CAP ASSEMBLY (MSE 43561-607.
TABLE 6 4 x 100 SWING OUT ROTOR (MSE 43121-302).3600 R.P.M. (MAX SPEED) ACCESSORIES 4 PLACE 15ml TRUNNION (34136-103), 15ml SPHERICAL END BUCKET (MSE 43156-601) SEALED BUCKET ADAPTOR TUBE CAPACITY 16 x 15ml j COLOUR CAP OPEN BUCKET MAX TUBE SIZE CUSHION RADIUS (MM) CAT. NO.
TABLE 7 4 x 200 SWING OUT ROTOR (MSE 43124-134). 4500 R.P.M. (MAX SPEED) (USED IN MISTRAL 2000) ACCESSORIES 200ml SEALED BUCKET (43551-121) SEALED BUCKET ADAPTOR TUBE CAPACITY COLOUR CAT. NO. 200ml BOTTLE 43551-103 4 x 200ml IV) CO CAP MAX TUBE SIZE OPEN BUCKET CUSHION DIA (MM) LENGTH (MM) 3631 57.7 120.2 RADIUS (MM) RCFxg 160.4 RADIUS (MM) RCFxg 4 x 100ml BROWN 34159-302 160.5 3637 39 113 4 x 50/1OZ YELLOW 34159-301 160.5 3637 29 113 28 x 15ml GREEN 34159-304 160.
TABLE 8 4 x 200 SWING OUT ROTOR (MSE 43124-134). 4500 R.P.M. (MAX SPEED) (USED IN MISTRAL 2000) ACCESSORIES 4 x 50ml UNIVERSAL BUCKET (43551-125) ADAPTOR SEALED BUCKET TUBE CAPACITY 16 x 50ml COLOUR CAP RADIUS (MM) CAT. NO.
TABLE 9 4 x 200 SWING OUT ROTOR (MSE 43124-134). 4500 R.P.M.
TABLE10 6 x 50 ANGLE ROTOR (MSE 43117-608). 6000 R.P.M. (MAX SPEED) MISTRAL 1000 (6500 RPM MAX SPEED MISTRAL 2000 [FIGURES IN BRACKETS]) ACCESSORIES 50ml SPHERICAL END BUCKET (MSE 43156-602) (6500 RPM MAX SPEED MISTRAL 2000) SEALED CAP ASSEMBLY (MSE 43561-162) SEALED BUCKET ADAPTOR TUBE CAPACITY COLOUR CAP CAT. NO.
TABLE11 6 x 50 ANGLE ROTOR (MSE 43117-608). 6000 R.P.M. (MAX SPEED) MISTRAL 1000 (6500 RPM MAX SPEED MISTRAL 2000 [FIGURES IN BRACKETS]) ACCESSORIES 50ml SPHERICAL END BUCKET (MSE 43156-602) (6500 RPM MAX SPEED MISTRAL 2000) SEALED CAP ASSEMBLY (MSE 43561-162) ADAPTOR SEALED BUCKET TUBE CAPACITY 6 x 15ml CO COLOUR CAP CAT. NO.
TABLE12 1 2 x 1 5 ANGLE ROTOR MSE 43117-607 6000 R.P.M. (MAX SPEED) MISTRAL 1000 (6500 RPM MAX SPEED MISTRAL 2000 [FIGURES IN BRACKETS]) ACCESSORIES 15ml SPHERICAL END BUCKET (MSE 43156-601) (6500 RPM MAX SPEED MISTRAL 2000) ADAPTOR SEALED BUCKET TUBE CAPACITY 12 x 15ml CO COLOUR CAP OPEN BUCKET MAX TUBE SIZE CUSHION RADIUS (MM) CAT. NO.
TABLE13 MICROTITRATION ROTOR (MSE 43121-117). 2600 R.P.M. (MAX SPEED) (USED IN MISTRAL 2000) ACCESSORIES SUPPORT TRAY (96500-345) MID TRAY CORNER TRAY NO.
14. Service Instructions HOW TO OBTAIN SERVICE ON YOUR MISTRAL 1000 OR 2000 CENTRIFUGE MSE is committed to giving our customers the best possible service. If your centrifuge should require service at any time please follow these procedures: - All countries except USA Contact your local MSE distributor. USA only For all technical enquiries contactFisons Instruments (ARL) Repair Center 15300 Rotunda Drive, Suite 301 Dearborn Michigan 48120.
WHAT YOU MUST DO 1. Contact our repair center - have the model, serial number, date of purchase and fault description available. 2. You will be given a return goods authorization number and directions for shipping. 3. Remove all rotors, buckets and adaptors. Do not ship these items - only the centrifuge. 4. Thoroughly clean and disinfect the centrifuge. 5. Fill out the attached service request form and place inside the centrifuge. 6.
MISTRAL SERVICE REQUEST We are sorry it is necessary to have your MSE centrifuge repaired. Please take a few moments to fill out this form which wil) help us ensure you receive the best and fastest service possibie. Model: Serial number (on plate at rear of unit): Date purchased: From: Brief description of fault: Date first noticed: Date Repair Center first contacted: ...
