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CE Conformity The following Gel Imaging Digital Systems, Models: ImageQuant-100, ImageQuant-300, ImageQuant-400, ImageQuant-ECL Are in conformity with the provisions of the following EC Directives, including all amendments, and national legislation implementing these directives: Low Voltage Directive 73/23/EEC EMC Directive 89/336/EEC And that the following harmonized standards have been applied: EN61010-1: 2001 EN61326-1: 1997+A1:1998+A2:2001 Class A EN55011 Class A, EN61000-3-2, EN61000-3-3, EN61000-4-2,
Table of Contents Chapter 1: Introduction and Setup ............................................................................................. 1 1.1 ImageQuant Capture Imaging System........................................................................ 1 1.1.1 Mouse Functions................................................................................................ 2 1.1.2 About This Manual ............................................................................................ 2 1.1.
2.1.8 Making Linear, Log, or Equal Adjustments .................................................... 41 2.1.9 Using the Contrast Adjustment Tools for Color Images ................................. 42 2.2 Tool Bar.................................................................................................................... 43 2.3 Tool Box................................................................................................................... 46 2.4 Status Bar....................................
4.1 The Zoom Tool......................................................................................................... 72 4.2 The Rotate / Flip Tool .............................................................................................. 73 4.3 Histogram ................................................................................................................. 74 4.4 Automatic Enhancement .......................................................................................... 76 4.
ImageQuant 300 Capture User Manual Chapter 1: Introduction and Setup 1.1 ImageQuant Capture Imaging System The ImageQuant Imaging System is a powerful digital imaging system, ideal for instant photography of a wide variety of samples. The CCD camera allows imaging of low-light samples in UV-illuminated, and fluorescent applications. The instrument is controlled by ImageQuant™ Capture software, which is designed with ease-of-use in mind.
ImageQuant 300 Capture User Manual 1.1.1 Mouse Functions The mouse supplied with the ImageQuant System has two buttons. The left button is used to activate functions and otherwise make selections when using the software. In some cases, the right mouse button is used to recall or reactivate the function that was most recently assigned to the left button. 1.1.
ImageQuant 300 Capture User Manual 1.2 ImageQuant Imaging System Setup 1.2.
ImageQuant 300 Capture User Manual 1.3 Computer, Camera and Peripheral Installation All software, peripheral drivers and operating systems have been pre-installed at the factory. During the system installation all components should only need to be plugged into the correct ports 1.3.1 Setting up the Power Strip/Surge Protector Turn the power switch on the power strip/surge protector off. Plug the power strip/surge protector into a wall outlet (preferably a dedicated circuit) and then turn the power on.
ImageQuant 300 Capture User Manual 1.3.2 Setting up the Computer, Monitor, Mouse and Keyboard (optional) The computer will need to have the monitor, mouse and keyboard connected to it in the correct ports (see the descriptions below). Also, a 3 prong standard power cable should be plugged into the back of the computer and the power strip.
ImageQuant 300 Capture User Manual 1.3.3 Software Driver Installation Instructions This set of instructions only applies if you did not purchase an integrated system and need to install the firewire card (If the computer contains a pre-installed firewire card or an on-board firewire port, please skip to step #4). 1. Install the firewire card into an available PCI slot on the motherboard of the PC. 2. Boot up the computer. 3.
ImageQuant 300 Capture User Manual 7. Open Device Manager and find the exclamation mark under Imaging Devices. Rightclick on Generic 1394 Desktop and select Update Driver: 8.
ImageQuant 300 Capture User Manual 9. Select Install from a list or specific location (Advanced): 10.
ImageQuant 300 Capture User Manual 11. Select Continue Anyway if a digital signature message appears. 12. After the driver installation is complete, the error message should be resolved in the device manager. 13. Exit out of the device manager.
ImageQuant 300 Capture User Manual 1.3.4 Camera Installation The ImageQuant camera connects via a standard IEEE 1394 firewire connection. If the system was shipped with a computer, the firewire card will be pre-installed. If the system was not shipped with a computer it will be necessary to install the firewire card first before installing the camera and software (see next section for driver installation instructions). 1. Locate the ImageQuant firewire camera cable: 2.
ImageQuant 300 Capture User Manual 1.3.5 Connecting the Printer (optional) The Mitsubishi P91DW (UB) printer connections are color coded for convenience. Plug the USB cable into the back of the printer and into the proper USB connector on the back of the computer. Plug in the standard 3 prong power cable to the back of the printer and to the power strip, then turn the power on.
ImageQuant 300 Capture User Manual 1.3.6 Cabinet Assembly (optional) When you remove the light cabinet from its shipping carton, it is already partially assembled. The camera mounting assembly is packed separately in the same container. The UV transilluminator and cabinet top are both packed in separate boxes. Make sure you have received all the hardware before discarding the shipping carton.
ImageQuant 300 Capture User Manual Installing the UV Transilluminator Take the following steps to install the UV transilluminator (this is a box-shaped UV lighting apparatus that rests at the bottom of the cabinet): 1. At the bottom of the cabinet, there is an extendable metal tray that is held in place by a single screw (this is to prevent the tray from being damaged during shipping). Start by removing this screw and then pulling the tray out so that it is fully extended. 2.
ImageQuant 300 Capture User Manual Unfastening the Fold-Down White Light Tray The cabinet contains a fold-down light tray that is locked for shipping (see illustration below). Take the following steps to unfasten the light tray: 1. The light tray is held in place by a detachable knob that screws into place just above the light tray. Unscrew the knob until it is separated from the cabinet and the tray can move freely. 2. Note the bottom of the tray has a threaded hole.
ImageQuant 300 Capture User Manual Attaching the Hood Take the following steps to attach the cabinet hood and connect the hood fan: 1. Place the hood on top of the cabinet so the holes for screws are centered over the corresponding holes in the top of the cabinet. 2. From within the cabinet, attach the hood fan’s power cord to the temperature board socket on the top- right side of the cabinet. 3. Fasten the hood to the cabinet using M4x16 socket head screws.
ImageQuant 300 Capture User Manual 1.3.7 Connecting the Cabinet (optional) The cabinet connects to the back of the computer via an RS232 cable. Connector on the back of the cabinet Connector on the back of the computer Ports on the back of the cabinet and computer respectively. Power on the cabinet after the Windows operating system has fully loaded. The ImageQuant Imaging System is now ready for use. 1.3.
ImageQuant 300 Capture User Manual 1.4 ImageQuant System Quick Guide Note: This is intended as a quick reference guide for acquisition. For more detailed information on the individual features reference section 1.6 of this manual. 1. Power on the system: a. Turn on the computer, monitor, and optional printer. b. After the computer has booted up completely to the Windows desktop, turn on the power to the cabinet (if applicable). c. Start ImageQuant Capture by double clicking on the desktop shortcut. 2.
ImageQuant 300 Capture User Manual a. Click on the Save Image function in the FILE menu or click on the SAVE or SAVE AS icon in the Tool Bar window. b. Enter a file name and select the directory to which it should be saved (the directory path should be less than 100 characters). c. Specify the file format (TIF, BMP, PCX, MAC, color TGA) d. Click OK to save the file 5. Enhance the display [optional] a.
ImageQuant 300 Capture User Manual 1.4.1 Cabinet Controls (for optional cabinet) Transillumination Controls These two buttons are used to toggle the UV transilluminator and the reflective white light table on and off. Reflective Light Controls These two buttons are used to toggle the reflective UV and white light sources on and off. UV Override Button Normally, if UV light is being used the cabinet automatically shuts off the UV light source when the cabinet is opened.
ImageQuant 300 Capture User Manual 1.5 System Information To display system information, select the About option in the Help menu. This button accesses a pop-up box. ABOUT Pop-Up Dialog This box shows the instrument serial number (where appropriate) and the Software version number. Use the information specific to your instrument and software when calling for technical support, software upgrades, etc. To close the dialog box, click on the OK button.
ImageQuant 300 Capture User Manual 1.6 Acquiring an Image In the TOOL BAR window, click on the Camera Acquire icon: Once the Camera Acquire Icon is pressed, the CAMERA SETUP AND REVIEW window is activated to provide exposure control of the camera, sensitivity/resolution controls, all lighting and filter wheel position controls, contrast display options and cabinet door open/close indicator using a software ‘virtual control’ menu. Also, FrameGrabber setup is controlled and activated from this window.
ImageQuant 300 Capture User Manual 1.6.1 Focusing on an Image Focusing on an image with ImageQuant Capture is easy with the real time display. Simply adjust the parameters on the manual lens, or, if the lens is controlled via the software, click on the appropriate software control button. 1. To open the aperture, turn the lens's aperture ring counter-clockwise for more light. Conversely, if the image is too bright, close the aperture by turning it clockwise until an image is visible on the monitor. 2.
ImageQuant 300 Capture User Manual 1.6.2 Control of the Optional Motorized Zoom Optics ImageQuant Capture controls the motorized optics when present. The software allows you to set the Aperture, Focus and Zoom (if applicable) from the software acquisition window. To change a value for the aperture, focus or zoom control, use the up and down arrows to control the settings.
ImageQuant 300 Capture User Manual 1.6.3 Auto Exposure and Auto Exposure Setup ImageQuant Capture’s acquisition window includes a check box and setup button for Auto Exposure. Auto Expose controls Auto Expose Setup options Auto Exposure Setup (Auto Exposure Compensation Setting): 4 choices available • Normal Exposure for image saturation: Use this choice for normal Colorimetric and Fluorescent imaging. • Under Exposure for chemilumenescence • Over Exposure for faint band detection.
ImageQuant 300 Capture User Manual exposure time in Preview mode, the software will begin acquiring at the last exposure time calculated during the preview—it does not start the calculation over from scratch.
ImageQuant 300 Capture User Manual 1.6.4 Cabinet Controls - Activating the Light Source and Selecting a Filter Use the mouse to click on the desired light source in the ‘virtual’ cabinet control interface or you can use the controls on the cabinet itself. Both mechanical and software controls are linked and communicate display setting selections. Note: There is a slight delay when the button is depressed until the light source is fully activated.
ImageQuant 300 Capture User Manual 1.6.5 Use Preview to Set Exposure Time Once the sample has been positioned and the camera has been focused, close the door to the ImageQuant light cabinet and make sure that the appropriate illumination source is turned on. Also make sure the cabinet door indicator in the Cabinet Control software interface indicates CLOSED. Click on the blue PREVIEW button and select the desired exposure time in the menu options to give the desired image intensity quality.
ImageQuant 300 Capture User Manual 1.6.6 Optimizing the Gray Scale for Saturation and Contrast Displays If an image will be analyzed, it is important that it not be over-saturated (too light) or under-exposed (too dark). Using Show Saturation, the user can see the areas of the image that are assigned to each end of the gray scale spectrum, and can adjust the imaging controls accordingly.
ImageQuant 300 Capture User Manual 1.6.7 Acquire and Transfer the Image Once the appropriate image is displayed on the screen, click on the CAPTURE button to capture and display the image in ImageQuant Capture. The acquired image will be automatically adjusted by a zoom factor based on the resolution setting to display the image optimally. From there, the image can be enhanced using ImageQuant Capture’s enhancement tools and/or transferred to an ImageQuant TL module for analysis.
ImageQuant 300 Capture User Manual 1.6.8 Capturing Images Using the FrameGrabber Mode If kinetic, multiplex, color, or chemiluminescence experiments are desired where you wish to have the system automatically capture several images at preset exposure times, preset time delay between images, preset lighting sources, and preset filter choices, the FrameGrabber tab can be selected in the Tool Box. (FrameGrabber setup is also accessible in camera setup acquisition screen.
ImageQuant 300 Capture User Manual Once FRAMEGRABBER is selected, a display box will appear for independent control of all lighting, filters, and exposure delay for each image frame. The TOTAL FRAMES setup provides you with the ability to determine how many individual frames (images) you want for the sequence. There is a maximum of 50 frames (images) and a minimum of 1 frame that can be captured with each sequence. The FRAME selection is used for setting up the conditions for each frame (image).
ImageQuant 300 Capture User Manual Once all images have been captured, the FrameGrabber tab will become displayed in the Tool Box. The buttons on this tab perform the following tasks: REC PLAY STOP PAUSE REW (rewind) REV (reverse) FWD (forward) DEL (delete) LOAD SAVE Opens the Camera Setup and Preview window to record a sequence. Display a continuous loop of all of the captured images.
ImageQuant 300 Capture User Manual 1.6.11 FrameGrabber: Save/Load FrameGrabber Setup Routines Two buttons “Save Setup” and “Load Setup” allow you to save and load all FrameGrabber setup parameters. Files are saved as *.mvf files. Load Setup and Save Setup buttons 1.6.12 Frame Stacking At the top of the Camera Setup and Preview window is an option for stacking frames.
ImageQuant 300 Capture User Manual 1.6.13 Capturing a Color Image Using the FrameGrabber Function A color image can be generated by acquiring three images each taken with a red, green and blue emission filter. Once saved, these images are then combined in the Overlay pull down menu. Open the image captured with each of the three filters as instructed and a RGB (red, green, blue) true color image will be generated.
ImageQuant 300 Capture User Manual Chapter 2: Getting Started - Basic Imaging Functions 2.1 Contrast Adjustment The Contrast Adjustment window allows for the best visualization possible of a sample utilizing the black, white, and gamma adjustments, as well as, image reverse and auto contrast. The image on the screen is made up of picture elements (pixels) in an array. Each pixel is assigned a brightness (or a gray scale value) level between black and white.
ImageQuant 300 Capture User Manual 2.1.1 Using the Contrast Adjustment Tools for Grayscale Images There are three sliding scales found in the image control area to the right of the image. Below each scale is a box displaying a number that corresponds to the position of the slider. By adjusting these sliding scales, the image display can be optimized.
ImageQuant 300 Capture User Manual Black Level set at 0 Black Level set at 60 2.1.3 White Level Adjustment The number beneath the White Level scale also corresponds to a gray scale value. When the slider is at the very bottom of the scale, this number is 255. As the slider is moved upwards along the scale, the number decreases and the image becomes progressively lighter. This is because all pixels at the specified gray level value and above are shown on the screen as white pixels.
ImageQuant 300 Capture User Manual Gamma set at 1.0 Gamma set at 0.55 2.1.5 The Auto Contrast Selection The Auto Contrast feature will automatically scale the black and white values of an image to more tightly fit the gray scale intensity profiles (histogram). This selection will use different black and white values for different images depending upon their unique histograms.
ImageQuant 300 Capture User Manual 2.1.6 The Reverse Button The Reverse button inverts the gray levels of the displayed image, converting a positive image to negative, or vice versa. For instance, an image with black bands on a white background is converted into an image with white bands on a black background by simply clicking the Reverse button. Clicking the button a second time returns the image to its original form.
ImageQuant 300 Capture User Manual 2.1.7 The Grid Button The Grid button provides an on-screen grid after image acquisition to check for proper sample alignment.
ImageQuant 300 Capture User Manual 2.1.8 Making Linear, Log, or Equal Adjustments Original image of film with default BWG settings Image of film with linear Contrast Adjustments selected. Linear provides minimum and maximum adjustment tools from 0 to 100%. Linear stretches the grayscale range of the displayed image to the maximum system dynamic range of 065,535 grayscales. Image of film with log Contrast Adjustments selected Log provides minimum and maximum adjustment tools from 0 to 100%.
ImageQuant 300 Capture User Manual 2.1.9 Using the Contrast Adjustment Tools for Color Images ImageQuant Capture can also import RGB color images. ImageQuant Capture automatically detects this process and the Contrast Adjustment tools are configured for color image adjustments.
ImageQuant 300 Capture User Manual 2.2 Tool Bar The Tool Bar window provides intuitive buttons for the most common functions in ImageQuant Capture. Tool Bar The Open button functions identically to the File Open function in the upper menu bar. This function is used to open previously saved images. Detailed instructions are available in Chapter 3. The Zoom Out and Zoom In buttons provide easy zooming ability while you are active in image enhancement functions providing increased versatility.
ImageQuant 300 Capture User Manual occurs. Saturation is a feature that is most important during the acquisition stages and is thoroughly detailed in the acquisition features of the system manuals. The Img Drag button is useful for to pan with a zoomed image. To activate this function, click on the button and move the mouse cursor to the image. The cursor will have changed to a small hand. Click the left mouse button and drag to move the image.
ImageQuant 300 Capture User Manual Clear removes any overlays currently displayed on the image. This function can be useful if annotations or other displays obscure parts of the image. Once an image is displayed, it can be printed on the default printer by clicking the Print button in the Tool Bar window display. Most printers can be configured through the Windows operating system to be the default printer. Refer to your Windows operating manual for more information on installing a default printer.
ImageQuant 300 Capture User Manual 2.3 Tool Box The Tool Box window contains an intuitive interface for performing all image enhancement functions. Tool Box It contains controls for enhancing and adjusting the image. This includes software filtering, false colors, zoom factors and other unique features. These tools are detailed in chapter 4 of this manual. 2.
ImageQuant 300 Capture User Manual Chapter 3: Pull-Down Menus Across the top of the screen is a Windows menu bar containing several system operation functions. These include file saving and loading, edit, image, setup, overlay, file utilities, view and help functions. ImageQuant Capture Menu Bar 3.
ImageQuant 300 Capture User Manual 3.1.1 File|Open This function opens an image that has been previously saved as a TIF, GLP, BMP, PCX, TGA, PIC, JPG or Macintosh® TIFF (MAC) file. File Open Dialog Box Using the left mouse button, click on the name of the file to be loaded. That name is then highlighted in the list and appears in the text box below the File Name prompt. Alternate disk drives can be accessed using the “Look In” dialog box.
ImageQuant 300 Capture User Manual 3.1.2 File|Overlay To superimpose images, use the OVERLAY function under the File menu. This function will display separate multiplexed images or a RGB color image as a compiled image with the appropriate color channel images added together. A simple way to acquire multiple images for this function is to use the FrameGrabber function in image acquisition and acquire a series of identical images.
ImageQuant 300 Capture User Manual 3.1.4 File|Save and Save As Save allows original images to be saved in several different formats. Save As allows images that have previously been saved to be saved in a different location or as a different file type without affecting the original image. ImageQuant Capture has the ability to save files in several formats, see the following figure: File Formats: *.tif, *.bmp, *.glp, *.pcx, *.tga, *.pic, *.jpg, *.
ImageQuant 300 Capture User Manual File Types TIFF is the default file format for ImageQuant Capture files. TIFF is an acronym for "tagged image file format" and was developed as a flexible and machine-independent graphic file format. Saving as a TIFF file will allow users to double-click TIFF files from Windows Explorer and automatically launch the application on any machine that has ImageQuant Capture loaded on it. Users may customize this in the preferences section covered in section 3.
ImageQuant 300 Capture User Manual 3.1.5 Printer Setup This function displays a dialog box in which the settings for the parallel printer are specified. When all the pertinent printing preferences have been specified, click on the OK button. If you purchased a printer with ImageQuant, this will be preset from the factory. Printer Setup Dialog Box Printer…. Dialog Box For more information on using the Print menu, see the Windows manual.
ImageQuant 300 Capture User Manual 3.1.6 Print This function sends the image to the default printer specified in Print Setup. 3.1.7 Logoff This function logs the current user out of ImageQuant Capture when security features are in use. 3.1.8 The Exit Function The Exit function closes ImageQuant Capture. To restart ImageQuant Capture from Windows, double-click on the ImageQuant Capture icon on the desktop.
ImageQuant 300 Capture User Manual 3.2 The Edit Menu The Edit menu provides the ability to copy, crop and remove any annotations or filters that have been added to the original image. Edit pull-down menu To activate the Copy and Crop functionality, place a check mark next to EDIT ACTIVATION. This will turn the mouse cursor into a + sign that will allow you to highlight the region of interest for the image. After Edit Activation is highlighted, the desired area of interest is drawn using the mouse.
ImageQuant 300 Capture User Manual Once this is completed, you can select either the COPY or CROP function in the EDIT menu options. COPY will copy the desired area of interest into the Windows Clipboard and allow you to paste into any desktop publishing package (i.e. Word, Excel, Adobe Photoshop, etc.). CROP will display just the region of interest as the active window in the ImageQuant Capture interface. ImageQuant Capture interface after cropped region has been selected 3.2.
ImageQuant 300 Capture User Manual 3.3 The Image Menu The Image menu option provides the ability to perform a variety of image processing functions. Image pull-down menu 3.3.1 Equalize The equalize option performs a duplicate function to the EQUAL option in the Contrast Adjustment Window. This is a useful function for detecting faint bands on a sample. 3.3.2 Arithmetic The Arithmetic function is used to add, subtract, average and divide several images together to generate a compiled image.
ImageQuant 300 Capture User Manual most common application for quotient is for those technical users who run their own flat field corrections. This can be done using the Flat Field Calibrate selection under the Image pull-down menu which will be described in detail later in this section. All three of these arithmetic functions are performed by opening the main image that will be adjusted. Next select the appropriate arithmetic function under the image pull-down menu.
ImageQuant 300 Capture User Manual 3.3.4 Flat Field Calibrate Flat Field Calibrate is a function that is used to ‘flatten’ the image so that the pixel data is even across the entire image area. This is a function that is useful for large gels and other applications that use the entire field of view for an image. Creating flats can be art in itself; there are many documents on the internet that can help users interested in this arena to create the ideal flat for the application.
ImageQuant 300 Capture User Manual 3.3.5 Image Resize The image resize function can be used to resize an image to a specific dimension for use in graphical presentations. You have the option to ‘Preserve aspect ratio’ to avoid image dimensional distortion, or you can deactivate this function and configure the image resolution to the desired Width and Height dimensions. Image Resize dialog box Note: It is recommended that you DO NOT perform quantitative analysis on resized images. 3.3.
ImageQuant 300 Capture User Manual 3.4 The Setup Menu This menu customizes the system settings by allowing users to save default parameter preferences and customize the software settings. Setup pull-down menu 3.4.1 Default Parameters Default parameter files eliminate the need to readjust the system settings each time the program is used, and can be especially useful if more than one person works with the program. The default file used by ImageQuant Capture when it initially loads is called IQcapture.def.
ImageQuant 300 Capture User Manual 3.4.2 Save Defaults Every time a default file is saved, all of the settings for the parameters listed above are saved. These can be loaded later and applied to any image. Save Defaults Dialog Box Once any of the system settings have been changed, a new default file can be created. To save a default file, enter a file name by typing in the text box below the File Name prompt.
ImageQuant 300 Capture User Manual 3.4.4 Security When the system is shared by a number of people or laboratories, a method of keeping track of its use may be helpful. The Security feature allows various levels of security and user log functions. We suggest one user be designated as the supervisor of the system. This individual should refer to Appendix B, which describes the security features in detail.
ImageQuant 300 Capture User Manual 3.4.6 Print Mode ImageQuant Capture provides custom printing options. Setup Print Image Info Dialog Box Printing can be achieved using three different methods: Full Image: Screen Dump: Image Window: Prints the original image. Does not print zoomed images or images overlaid with data screens. Prints the imaging area. Well suited for printing images overlaid with data screens and/or graphs, zoomed images, etc. Prints the highlighted window. 3.4.
ImageQuant 300 Capture User Manual There are four tabs in the Preferences menu (ImageQuant Capture standalone software does not contain the Image Acquire or Cabinet Settings tabs): 1. General – Configure prompts and file saving/opening formats. 2. Image Acquire – Inverts the image seen by the camera and adjusts the ROI values.
ImageQuant 300 Capture User Manual 3. Cabinet Settings – Used for adjusting the port settings and customizing filter positions on the cabinet. 4. Auto Enhancements – Used to customize the Auto Enhance Levels located in the tool box. To make changes to the preferences, select your change by typing in a new value or select/de-select the appropriate box with a check mark and then select apply. Some settings may require that the software be restarted before the change will take effect.
ImageQuant 300 Capture User Manual 3.5 The Overlay Menu The Overlay menu provides a means of saving and retrieving annotation overlays. This is especially useful when a standard gel format is run repeatedly. Lane numbers, molecular weight marker sizes, and other pertinent information can be stored as an Overlay file and retrieved at a later date. This eliminates the need to re-enter the information each time a new image is captured.
ImageQuant 300 Capture User Manual The current directory is the one in which the new overlay file will be saved. necessary, change the directory or drive as described in Section 3.1. If Once a name has been entered and the appropriate directory has been accessed, click the SAVE button to save the overlay file. 3.5.2 Loading an Overlay The Load Overlay function allows Overlay files to be retrieved and applied to the image currently displayed.
ImageQuant 300 Capture User Manual 3.6 The Utilities Menu A number of functions are now handled by Windows programs. To access many of these programs while in ImageQuant Capture, open the Utilities menu and select the program of choice. Utilities pull-down menu 3.6.1 Explorer This option can be used as a shortcut to Windows Explorer, which allows access to files and other information saved on the local machine or the network. 3.6.
ImageQuant 300 Capture User Manual 3.7 The View Menu The View function provides the ability to control the display of the on-screen control tools as well as provide image enhancement abilities.
ImageQuant 300 Capture User Manual Tool Box Window Status Bar These tools are automatically displayed when ImageQuant Capture is launched for additional ease of use and to generate a common ‘look and feel’. However, if you would like to hide any of these tools they can be turned off in the View menu by just deactivating the check mark next to the item that you would like to remove from the screen.
ImageQuant 300 Capture User Manual 3.8 The Help Menu Help pull-down menu 3.8.1 On-Line Note On-line help is available from the ON-LINE NOTE section of the help menu. 3.8.2 About To display system information, select the About option in the Help menu. This button accesses a pop-up box. This box shows the system serial number and software version number. Use this information when contacting technical support, upgrading software, etc.
ImageQuant 300 Capture User Manual Chapter 4: The Tool Box Image enhancement tools are contained within the Tool Box as indicated. This tool set allows the user to zoom the image, rotate-flip the image, show the image histogram, perform automatic image enhancement, annotate on the image, display false colors, apply software filters, and activate the FrameGrabber function. 4.1 The Zoom Tool The Zoom Tools The Zoom tool is found in the Tool Box.
ImageQuant 300 Capture User Manual 4.2 The Rotate / Flip Tool The Rotate / Flip tools are found in the Tool Box. This function rotates the image in a clockwise or counterclockwise direction by 1 degree increments up to a maximum of 90 degrees in either direction. This is a useful tool if the image is not aligned properly during the capturing process.
ImageQuant 300 Capture User Manual 4.3 Histogram The histogram is a graphical display of the proportion of pixels assigned to each of the image’s gray levels. This tool is found in the Tool Box. Histogram display in the Tool Box The image is made up of picture elements (pixels) having brightness levels ranging from black to white. A very bright image will have most of its pixels registering high gray levels and conversely, a very dark image will have most pixels with low gray levels (approaching zero).
ImageQuant 300 Capture User Manual that the gray level range be as large as possible. If an image does not include most of the gray levels, we recommend repeating the image capturing process.
ImageQuant 300 Capture User Manual 4.4 Automatic Enhancement The Enhance tool is found in Tool Box. This function is ideal for new or inexperienced users of the system since it offers 9 levels of automatic image enhancement of the black, white, and gamma levels simultaneously. For an inexperienced user, it can be difficult to adjust each black, white, and gamma buttons to their respective optimal positions.
ImageQuant 300 Capture User Manual 4.5 Annotations The annotation tools, found in the Tool Box, include a number of different options for adding text (including Greek symbols), drawing arrows and otherwise marking an image. Note that these tools are for annotation only. Annotations Tool Box 4.5.1 Object Attributes Use the COLOR, PEN WIDTH, PEN STYLE, LINE ENDS, TEXT STYLE and/or TEXT ORIENT menus to specify object attributes. Attributes can be assigned to the cursor before drawing or typing.
ImageQuant 300 Capture User Manual 4.5.3 Line Thickness The PEN WIDTH menu specifies the thickness of lines when using the freehand, lines, box and circle drawing tools. Click on the appropriate checkbox for the desired width. All annotations subsequently entered will appear at that width. Pen Width Selection Tools 4.5.4 Line Types The PEN STYLE menu specifies the style of lines when using the freehand, lines, box and circle drawing tools. Click on the appropriate checkbox for the desired style.
ImageQuant 300 Capture User Manual 4.5.6 Text Background and Font The TEXT STYLE menu specifies the style of text. Click on the appropriate checkbox to show text with or without a background. An opaque background is useful if annotations will be made on an image that has wide variations in gray scale. By using an opaque background, text will not be “lost” in the background of the image. Text Style Selection Tools This is also the window in which specific font is chosen.
ImageQuant 300 Capture User Manual 4.5.7 Text Angles In the TEXT ORIENT window, select whether text should be oriented vertically, horizontally, or at an angle (in 15° increments). Text Orient Selection Tools Note: only rotate fonts that are True Type (indicated by TT in front of the name); other fonts (such as Courier and Fixedsys) do not re-scale properly, giving unpredictable results. 4.5.
ImageQuant 300 Capture User Manual The button labeled with a diagonal arrow draws arrows and straight lines. After clicking on the button, move the cursor to the position on the image where the line should begin. Press and hold the left mouse button. Using the mouse, move the cursor to the other end point of the line then release the mouse button.
ImageQuant 300 Capture User Manual Annotated image showing: freehand drawings, lines with various characteristics, circles, squares, text with various characteristics 4.5.9 The Editing Tools When the cursor is in “edit” mode, it can be clicked on an object to select it. (Note: the cursor can be toggled between “edit” and “drawing” modes by clicking the right mouse button.) When an object is selected, small square boxes appear at the corners.
ImageQuant 300 Capture User Manual Once an object or group of objects has been selected, clicking on the Cut tool deletes it from the image. The Copy tool makes an exact copy of the selected object. The new object becomes the selected object, and can be repositioned by placing the cursor within the object’s boundary and moving it to the desired location. The Horizontal Alignment tool aligns annotations in a straight horizontal line. This is especially useful for labeling lanes, etc.
ImageQuant 300 Capture User Manual 4.6 False Color These tools consist of eleven pre-defined color palettes that can be applied to an image. To select a palette, simply click on one of the four buttons labeled GRAY PALETTE, SATURATION, NEXT or PREVIOUS. False Color Selection Box When a palette is selected, its range of colors is displayed to the left of the palette buttons and automatically applied to the image. To apply a different palette to the image, click the NEXT or PREVIOUS buttons.
ImageQuant 300 Capture User Manual 4.6.3 Palettes 2 through 11 These are color substitution palettes in which the gray levels are translated into different color ranges. These palettes can be useful to help distinguish features and highlight details on an image. Palette 2 maps the gray scale levels to a red/green/blue palette. Values of 0 are mapped to red; saturated to blue, and values in between to green. Palette 3 maps the gray scale levels to a red/green/blue palette.
ImageQuant 300 Capture User Manual 4.7 Image Filters ImageQuant Capture includes a variety of enhancement filters that can improve the appearance of an image. Some filters sharpen details, others smooth and reduce random noise. Still others help visualize edges and separate closely spaced bands or objects. Depending upon the unique characteristics of an image, the results of each filtering operation vary.
ImageQuant 300 Capture User Manual 4.7.1 General Information on How Filters Work To enhance an image, filters change the value assigned to each pixel. The new value assigned to a pixel is determined based on the values of the other pixels in its local vicinity (or “neighborhood”). The neighborhood is a two-dimensional matrix of pixel values, where each dimension has an odd number of elements. The "pixel of interest" is the one at the center of the neighborhood.
ImageQuant 300 Capture User Manual 4.7.2 Sharpening Filters These filters can increase image sharpness and provide edge enhancement. However image noise may be enhanced as well. These filters accentuate the high-frequency details of an image while leaving the low-frequency content intact. High frequency portions of the image get brighter while low frequency portions become black. Sharpen level 9 (high) has the largest effect on the image. Sharpen level 5 has an intermediate effect.
ImageQuant 300 Capture User Manual In addition, 3-D allows the user to control the ‘direction’ of the 3-D shadowing effect. Once the level is refined, just click on a direction arrow to visualize the shadowing adjustment. The default shadow direction is in the lower right hand corner direction and 9 different directions are available. Original Image 3-D Image Level 5 & lower right shadow 3-D Image Level 5 & upper middle shadow Original Filter Tool Box Lower right shadow Upper middle shadow 4.7.
ImageQuant 300 Capture User Manual which is an area with large changes in intensity, the subtraction yields light-colored pixel values. The larger the difference in intensities, the lighter the resultant pixels. Note: After applying the horizontal edge filter the entire image may appear black, and might require reducing the White level in order to better visualize the results. 4.7.9 Vertical Edge Filter This filter (Vert.
ImageQuant 300 Capture User Manual 4.7.
ImageQuant 300 Capture User Manual 4.8 FrameGrabber If kinetic, multiplex, color, or chemiluminescence experiments are desired where you wish to have the system automatically capture several images at preset exposure times, preset time delay between images, preset lighting sources, and preset filter choices, the FrameGrabber tab can be selected in the Tool Box. (FrameGrabber setup options are also accessible in camera setup acquisition screen.
ImageQuant 300 Capture User Manual Once FRAMEGRABBER is selected, a display box will appear for independent control of all lighting, filters, and exposure delay for each image frame. The TOTAL FRAMES setup provides you with the ability to determine how many individual frames (images) you want for the sequence. There is a maximum of 50 frames (images) and a minimum of 1 frame that can be captured with each sequence. The FRAME selection is used for setting up the conditions for each frame (image).
ImageQuant 300 Capture User Manual Once all images have been captured, the Tool Box will automatically display the FrameGrabber tab. The buttons on this tab perform the following tasks: REC PLAY STOP PAUSE REW (rewind) REV (reverse) FWD (forward) DEL (delete) LOAD SAVE Opens the Camera Setup and Preview window to record a sequence. Display a continuous loop of all of the captured images.
ImageQuant 300 Capture User Manual 4.9 FrameGrabber: Save/Load FrameGrabber Setup Routines Two buttons “Save Setup” and “Load Setup” allow you to save and load all FrameGrabber setup parameters. Files are saved as *.mvf files. Save/Load Setup buttons 4.9.1 Frame Stacking At the top of the FrameGrabber setup window is an option for stacking frames. If this selection was chosen during acquisition of the image Stack Frames will use all previous exposure information to sequentially add images to one another.
ImageQuant 300 Capture User Manual 4.9.2 Capturing a Color Image Using the FrameGrabber Function A color image can be generated by acquiring three images each taken with a red, green and blue emission filter. Once saved, these images are then combined in the Overlay pull-down menu. Open the image captured with each of the three filters as instructed and a RGB (red, green, blue) true color image will be generated.
ImageQuant 300 Capture User Manual Chapter 5: Analyzing Images Once you have acquired an image using ImageQuant Capture, you can transfer it to one of ImageQuant TL’s analysis modules using the toolbar options. The ImageQuant Capture toolbar contains 4 buttons: 1D, Array, Colony and Toolbox: 1D This is a gel or TLC plate analysis module. Array The Array module to analyze microplate images, gridded arrays, and dot and slot blot images.
ImageQuant 300 Capture User Manual Appendix A: Opening ImageQuant Capture Files in Other Software Programs Files generated by ImageQuant have been tested in the software packages below. For successful imports, the command line is given. Programs for the Macintosh® operating system were tested on a PowerMac® 8100/100AV. Results may vary for different software versions and/or hardware configurations. Adobe Photoshop 2.51 LE (Mac) Adobe Photoshop 3.0 (Mac) Canvas 3.5 (Mac) Microsoft Word 6.
ImageQuant 300 Capture User Manual Using ResEdit 1. Save image in IS-1000/500 as a TIFF or MACTIFF 2. Obtain a copy of the freeware ResEdit by downloading from Apple Computers through the Internet. 3. Open ResEdit. An animated startup display will show up and continue until you click on the mouse or any key. 4. A dialog box will appear. Open the TIFF image. Another dialog box will appear asking if you want to add a resource fork, click on ‘OK.’ 5.
ImageQuant 300 Capture User Manual Appendix B: Security Features This feature should only be used by the purchaser or supervisor of the system. Remove this page from the manual and store it in a safe place. When the system is shared by a number of users, security features may be useful for regulating or maintaining a log of instrument use.
ImageQuant 300 Capture User Manual NOTE: You must exit and re-launch ImageQuant Capture in order for any changes to take place. If you decide not to edit the security settings at this time, simply click on the EXIT button to dismiss the dialog box. Setting Various Security Levels A variety of security levels are available. Each is described below, in increasing order of security. Also shown are the proper settings in the dialog box to choose each security level.
ImageQuant 300 Capture User Manual Each time a new name is used, it is added to the master user list. Since this list requires a password (in case the system is later changed to a closed, password protected system), ImageQuant will automatically assign the user’s name (in uppercase) as the password, and the password field is disabled. When finished using ImageQuant, the user can choose Logoff from the File menu, or can log out by exiting the system.
ImageQuant 300 Capture User Manual Option 4: Closed System with User Name Log In and Out A user can log in only by entering an authorized name. A user log is generated. In closed systems, the system supervisor may want to add or remove names from a list of authorized users. To do this, click the Add/Remove Users button. A dialog box will appear with a list of all the current users.
