User`s manual

Chapter 5: Analysis Tools
5-9
• The position corresponds to the band's location along the y-axis, ranging from 0 to
1030.
• The value displayed under the Mol. Wt. heading is the molecular weight value
entered for a known band or the value calculated for an unknown band.
• The R
f
value for each band is also displayed. Unless otherwise specified,
AlphaEaseFC assumes the origin (R
f
value = 0.00) is located at the top of the screen,
and dye front (R
f
value = 1.00) is at the bottom of the screen. Using these points as a
frame of reference, AlphaEaseFC calculates R
f
values for the intermediate bands.
Molecular Weight Marker and Query Data
If the data box obscures part of the image, it can be resized or moved using Windows
®
functions, or it can be hidden using the Hide Data checkbox.
Repositioning and Deleting Markers
Marker band indicators can be repositioned simply by positioning the cursor, clicking,
and dragging the line to the desired location.
To delete a molecular weight marker, point the cursor at the appropriate marker band and
click the left mouse button. This highlights the band in question by putting a red box
around it. (Alternatively, clicking in the data table highlights the marker’s information
and selects the band on the image.)
Click on the Delete Marker function in the Marker menu. The marker is deleted, as is
the band's data in the marker data table. Markers entered after the deleted bands are
renumbered on the image and the data table.