User`s manual

Alpha Innotech User’s Manual, version 4
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menu. To change the background color of the graph, choose Background Color from
the Options pull down menu.
AlphaEaseFC automatically detects the peaks and integrates the area under each peak.
Adjustments that can be made manually are described below.
The Data Window
Once a peak is defined, its integration data and associated information are displayed in a
table located in the lower right quadrant of the screen. The data table is updated any time
a peak is deleted or added, peak boundaries are redefined, or the background value is re-
set.
Example of a Quantitation Data Table
PEAK is the number assigned to each peak on the graph beginning on the left and
moving right.
DIST is the distance (in pixels) that each peak starts from the beginning of the line scan.
WIDTH and HEIGHT refer to the size of each peak.
AREA is the integrated area under each peak and represents band intensity.% is the
percentage each peak contributes to the total density. (The sum of this column will be
100% for each lane).