User`s manual

Alpha Innotech User’s Manual, version 4
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Using the V.LINE
The line also contains hash marks, which represent pixel intensities. Use this information
for a quick comparison of peak heights. Note: The hash marks are most useful when
only four graphs are shown so that enough detail is displayed. To accomplish this, click
on the selection button in the upper left corner of the window and deselect Show All
Lanes.
Alternatively, the intensity value at the point where the V.Line crosses the graph can be
displayed. Hold the <shift> key and click the right mouse button to toggle between
intensity values and hash marks.
3-D View
The 3-D View feature changes the appearance of the graphs to a 3-dimensional display.
This can be useful in understanding how data was obtained, and in visualizing any
anomalies that occur on the gel.
3D Scan
The depth of the display is determined by the Scan Width (i.e. the above scan has a
width of 35 and is displayed in 3-D mode as 35 graphs). This function acts like a toggle:
click in the box next to 3-D View to activate the function; click in the box again to
deactivate it and return the graphs to their original appearance.
Note: If a lane has been scanned with a large width, it may take a long time to
display the graph in 3D mode. If this is the case, select 3D View: Show Hidden
Lines from the utility menu in the graph window. This will result in a graph that is
not as “clean,” but which displays on the screen very quickly.
Smooth Data