User`s manual

Alpha Innotech User’s Manual, version 4
5-42
Vertical Lanes or Horizontal Lanes
If the gel image has vertical lanes the Vertical Lanes option should be checked. If the
image’s lanes run horizontally then the Horizontal Lanes option should be chosen. The
software will not automatically detect the lane orientation so the user should aware of this
parameter.
Sensitivity Adjustment Bar
This feature changes the number of bands the software will find using a sensitivity scale
of 0-9. The lower the number (slider bar left) the less bands the software will find, and
the greater the number (slider bar right) the more bands the software will find. Upon
changing the sensitivity, Find Bands must be selected for the software to “re-find” the
bands.
Area of Interest Drawing
It is recommended that the user draw an area of interest on the image in order for faster
and more accurate detection of lanes and bands. An area of interest is drawn using the
left mouse button to click and drag (draw) a box shaped region around the sample area.
Image artifacts ( well position marks etc) should be left out of the area of interest as the
software may record these as bands. After an area of interest is drawn the Find Lanes
button should be selected. The software will automatically find lanes and bands within
each lane. An area of interest need not be drawn for the automatic detection Find Lanes
to work. The analyzed image and overlaid data table will now appear.
Analyzed Image