User`s manual

Alpha Innotech User’s Manual, version 4
5-46
Baseline
Background subtraction can be preformed using this menu. (See Auto Grid Baseline
Control described previously in this section for detailed instructions). Please note that
background subtraction in AutoLane can have a dramatic effect on how many peaks are
recognized as bands. Try changing the background subtraction to optimize band
recognition.
Options
Background Color allows the user to select a variety of different background colors for
the Active Graph. Axes allows the user to display the axes on the graph (x axis = pixel
distance down the lane, y axis = pixel intensity in gray scale). Show Strip will display a
strip of the lane being currently analyzed. Select Window will allow the user to choose
which of the data table windows to display. Print will allow the user to print any or all of
the profile and data table windows. Clipboard will allow the user to send any or all of the
profile and data table windows to the clipboard for pasting into different programs (eg
Excel™).
Options drop down menu
Editing Peak Boundaries
The boundaries of existing peaks can be readjusted to make the peaks wider or narrower.
To adjust either peak boundary, click on the peak to select it. Next, point the cursor at
the vertical boundary line, hold down the left mouse button and drag the line to the
desired location. When it is in place, release the mouse button.
The data table is automatically updated to reflect any adjustments. Both the peak and the
data in the data table will be blue to indicate that this is a user-defined (vs. automatically
detected) peak.