User`s manual

Alpha Innotech User’s Manual, version 4
5-60
Grab Tool
The grab tool allows the user to navigate the entire dendrogram in a zoomed display by
clicking and dragging to window.
Zoom In/Out Tools
The zoom in and out tools allow zoom control over the dendrogram window.
Fit to Window Tool
Selecting this button will fit the dendrogram to the current window size.
5.4 Spot Density Tools
In the ToolBox, Analysis Tools, a tab labeled SPOT DENSO, opens a set of tools with
which the density of bands, spots or other objects can be measured. A two dimensional
area of interest (or Object) is created and the density is obtained through the
corresponding pixel intensity values designated as IDV or Integrated Density Value.
Spot Densitometry Tools
Creating an Object Area of Interest
Object Boxes:
An area of interest can be created in several ways. The user can manually draw an area
of interest through the three OBJECT buttons. Objects can be enclosed with a box
(rectangle), ellipse, or freehand drawing. Select the one that most closely corresponds to
the shape of the objects on the image.
To draw a rectangle or ellipse, click on the button labeled with a box (or a circle) beneath
the OBJECT header in the toolbox. The cursor automatically changes to a "+" when it is