User`s manual

Alpha Innotech User’s Manual, version 4
5-74
To remove a band from the set of standards, click on it again. The band number changes
from green back to white, indicating that it is no longer a standard. To de-select all the
standard points at once, click on the CLEAR STD PTS button in the toolbox.
To change the value of a standard band, click on it twice. In the dialog box, the estimated
value for that band is displayed. Accept this value by clicking OK, or enter a new value.
Displaying the Curve
If the box displaying the standard curve obscures a part of the image, it can be resized
using Windows
®
operating system functions. It can be hidden from view by clicking the
HIDE CURVE checkbox in the Standard Curve toolbox.
Quantitation Values of Unknown Bands
As the values for the standard bands have been entered, the quantitation values of the
unknown bands are automatically calculated. To see the calculated values of the
unknowns, click on the EXIT button in the standard curve toolbox. This returns to the
Spot Denso tools and data box.
Note: the graph is based on a Smoothed Cubic Spline fit. To use other curve-fitting
methods, output the data table as an ASCII file and open it in any statistical analysis
package. (See Outputting Quantitation Data below.)
Spot Denso Data Box
The third column in the data box contains values for the standards (entered by the user),
and the values for the unknown bands (calculated based on the curve). The heading of
this column reflects the units entered as in Specifying the Quantitation Units above.
An "n" next to the object number indicates that the objects are unknowns (or non-
standards) whose value has been interpolated. An “a” means that the object lies outside