User`s manual

Chapter 5: Analysis Tools
5-79
The Sensitivity indicator determines how close the cursor must be to a marker in order to
delete it. Higher numbers allow the user to click further away from the marker to delete
it; however, if Sensitivity is set too high, it will be impossible to place markers on spots
that are close together.
Hiding Your Count Markers from the Screen
The software will record all counts as you continue clicking on your objects. If the
counting becomes confusing, you may click on the HIDE button to hide off of the x’s and
+’s that were used to count your objects on the screen. If you wish to make a print of
your image hiding the count, make sure that the HIDE button has been pressed to high
your count markers. You can then go to Tool Box, Enhancement Tools, Annotations,
and label the image with the appropriate. Click on the PRINT button to obtain a
hardcopy.
Erasing the Count Markers and Data
Once you have finished counting your objects and have obtained the data you need, you
may clear the count by clicking on the ZERO button. This will erase all of the count
markers from the screen and zero the count data to allow you to begin counting again
with the same image or another image that you have acquired.
Automatic Count
Follow these three steps to perform an automatic object count:
1. Define and Position Area(s) of Interest
There are three drawing tools with which areas of interest can be designated. Click on the
desired AOI (Area of Interest) button in the toolbox to define areas on the image
containing the objects to be counted.
AutoCount Tools
Circles: This is the most appropriate choice for counting colonies on a Petri dish. After
clicking on the button in the toolbox labeled with a circle, move the cursor into the image
area to the edge of the Petri dish. Click the left mouse button and move the cursor to