User`s manual

Alpha Innotech User’s Manual, version 4
5-86
5.6 The Scoring Function
The Scoring function is designed for gene expression applications to check for presence
or absence of specific samples. The scoring function can be used for a quick manual
identification of several different sample types. The software allows for up to three
different scores on a gel, blot, or microtiter plate.
To score an image, click on the SCORING button in Toolbox 4. Define the appropriate
number of rows and columns of your image by using the appropriate controls. You can
also define the amount of spacing between your rows and columns to compensate for any
variations between lanes or wells.
Scoring controls
Once you have selected the number of rows and columns to be displayed, you may adjust
the template to fit your image. Use the left button on the mouse to click, hold, and drag
the outside borders of the template so they frame the lanes or wells to be scored.
Clicking, holding, and dragging on or within any border moves the entire template.
Clicking on the blue corners surrounding the template resizes it.
Scoring image with template positioned