User`s manual

Alpha Innotech User’s Manual, version 4
1-21
Once MOVIE SETUP is selected, a display box will appear for independent control of
all lighting, filters, and exposure delay for each image frame.
The TOTAL FRAMES setup provides you with the
ability to determine how many individual frames
(images) you want for the movie. There is a maximum
of 50 frames (images) and a minimum of 1 frame that
can be captured with each movie.
The FRAME selection is used for setting up the
conditions for each frame (image). For example, if three
(3) images are to be captured, you would choose
FRAME 1 and setup all of the desired lighting and filter
requirements. You can then click on FRAME 2 and
repeat the above. Or, you can click on COPY TO
NEXT to help speed up the setup process. COPY TO
NEXT copies all settings from the previous frame to the
current frame. Usually, for chemiluminescence
imaging, all lighting is off and the filter wheel is
positioned for the chemiluminescence position for all
frames. Thus, the only variable that is changing from
one frame to the next is the exposure time. In this
situation, COPY TO NEXT is a useful tool to save time
in the setup process.
If you are performing kinetic experiments where you
want to have a predetermined delay between captured
images, then you can use EXP DELAY to configure
this function. The default EXP DELAY is set for the shortest possible delay (19
milliseconds), but can be configured up to 50 minutes between each image. Also, if your
exposure delay and/or exposure time and/or lighting options/filter position is consistent
for the entire movie, then once you setup the first frame, you can select the COPY TO
END selection to automatically choose the first frame settings for the entire movie of
frames (images).
Once the movie is setup to the desired configuration, click on the GO button. The movie
will then begin the image acquisition for each frame of the image. When it is complete,
the movie setup box will disappear and the TOOLBOX window will automatically
configure to the MOVIE tools. This will allow you to playback the movie, save or load
the movie, or record a new movie.