User`s manual

Alpha Innotech User’s Manual, version 4
3-10
3.3 The Image Menu
The Image menu option provides the ability to perform a variety of image processing
functions.
Image Pull down menu
Equalize
The equalize option performs a duplicate function to the EQUAL option in the Contrast
Adjustment Window. This is a useful function for detecting faint bands on a sample.
Arithmetic
The Arithmetic function is used to add, subtract, average and divide several images
together to generate a compiled image.
Image Arithmetic dialog box
To average a set of images together open one of the images in the set and then select
‘Average a Set…’ under the Image pull down menu. A prompt will appear allowing
the user to select all of the images that for the set. It is possible to browse the directories
looking on the network drives and removable media if necessary. Once all of the images
have been selected click on the open button to finish the set. The resulting image is an
average of all of the images together. This is a useful function for extending the dynamic
range on a set of similar images by allowing bright spots and faint spots to be seen on the
same image.
The other functions are adding, subtracting and dividing images together. Adding
together images is frequently used for colorimetric markers run together with